(Circular) (Six-base) MAPSORT of: pFN21AB9101.seq Check: 1235 from: 1 to: 8179 pFN21AB9101 8179 bp With 182 enzymes: SgfI * February 27, 2009 14:34 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 4390 4390 Size: 8179 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 5989 278 Size: 53 83 186 936 4453 2468 Fragments arranged by size: 4453 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 5522 1937 Size: 3585 4594 Acc65I G'GTAC_C Cuts at: 5500 5500 Size: 8179 AclI AA'CG_TT Cuts at: 1563 3875 6306 6679 1563 Size: 2312 2431 373 3063 Fragments arranged by size: 3063 2431 2312 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2399 6242 7626 7894 1632 Size: 767 3843 1384 268 1917 Fragments arranged by size: 3843 1917 1384 767 268 AflII C'TTAA_G Cuts at: 820 1017 4201 820 Size: 197 3184 4798 Fragments arranged by size: 4798 3184 197 AflIII A'CryG_T Cuts at: 1203 7078 8055 1203 Size: 5875 977 1327 Fragments arranged by size: 5875 1327 977 AgeI A'CCGG_T Cuts at: 7010 7010 Size: 8179 AhdI GACnn_n'nnGTC Cuts at: 2122 4136 6908 2122 Size: 2014 2772 3393 Fragments arranged by size: 3393 2772 2014 AleI CACnn'nnGTG Cuts at: 1166 4310 1166 Size: 3144 5035 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2460 2505 1184 Size: 1276 45 6858 Fragments arranged by size: 6858 1276 45 AlwNI CAG_nnn'CTG Cuts at: 2108 3365 4085 5084 5922 7494 2108 Size: 1257 720 999 838 1572 2793 Fragments arranged by size: 2793 1572 1257 999 838 720 ApaLI G'TGCA_C Cuts at: 2036 3858 4313 6236 7392 2036 Size: 1822 455 1923 1156 2823 Fragments arranged by size: 2823 1923 1822 1156 455 ApoI r'AATT_y Cuts at: 1493 2323 3469 3736 3901 4296 4706 4727 4928 Size: 830 1146 267 165 395 410 21 201 Cuts at: 4928 5488 5714 5866 7975 1493 Size: 560 226 152 2109 1697 Fragments arranged by size: 2109 1697 1146 830 560 410 395 267 226 201 165 152 21 AseI AT'TA_AT Cuts at: 160 1032 6733 160 Size: 872 5701 1606 Fragments arranged by size: 5701 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 8179 AvaI C'yCGr_G Cuts at: 1870 1943 2844 5504 1870 Size: 73 901 2660 4545 Fragments arranged by size: 4545 2660 901 73 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 2097 2130 2097 Size: 33 8146 BamHI G'GATC_C Cuts at: 3418 5509 5876 3418 Size: 2091 367 5721 Fragments arranged by size: 5721 2091 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 5500 6955 618 Size: 325 217 627 3713 1455 1842 Fragments arranged by size: 3713 1842 1455 627 325 217 BanII G_rGCy'C Cuts at: 1391 5498 1391 Size: 4107 4072 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2262 4407 928 Size: 971 363 2145 4700 Fragments arranged by size: 4700 2145 971 363 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 2825 2859 6370 6404 1903 Size: 34 888 34 3511 34 3678 Fragments arranged by size: 3678 3511 888 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 6071 7281 6071 Size: 1210 6969 BclI T'GATC_A Cuts at: 1548 1548 Size: 8179 BfrBI ATG'CAT Cuts at: 2083 2083 Size: 8179 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 6790 136 Size: 107 122 71 631 605 5118 1525 Fragments arranged by size: 5118 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 8174 8174 Size: 8179 BlpI GC'TnA_GC Cuts at: 4753 5596 4753 Size: 843 7336 Bme1580I G_kGCm'C Cuts at: 1790 2040 3862 4317 4342 4663 6240 7396 1790 Size: 250 1822 455 25 321 1577 1156 2573 Fragments arranged by size: 2573 1822 1577 1156 455 321 250 25 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 8179 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3627 5106 5910 Size: 416 53 83 399 24 2210 1479 804 Cuts at: 5910 6868 442 Size: 958 1753 Fragments arranged by size: 2210 1753 1479 958 804 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 2354 3021 1056 Size: 1298 667 6214 Fragments arranged by size: 6214 1298 667 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2044 2044 Size: 8179 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 3636 4127 6839 1239 Size: 2397 491 2712 2579 Fragments arranged by size: 2712 2579 2397 491 Bpu10I CC'TnA_GC Cuts at: 3174 5049 3174 Size: 1875 6304 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2459 4339 6304 7169 7467 2459 Size: 1880 1965 865 298 3171 Fragments arranged by size: 3171 1965 1880 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 3699 3943 6842 882 Size: 617 2200 244 2899 2219 Fragments arranged by size: 2899 2219 2200 617 244 BsaAI yAC'GTr Cuts at: 493 1204 5970 493 Size: 711 4766 2702 Fragments arranged by size: 4766 2702 711 BsaBI GATnn'nnATC Cuts at: 2607 3264 8173 2607 Size: 657 4909 2613 Fragments arranged by size: 4909 2613 657 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 5986 6368 7003 275 Size: 53 83 186 936 4453 382 635 1451 Fragments arranged by size: 4453 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 6612 7010 7284 7431 7842 6612 Size: 398 274 147 411 6949 Fragments arranged by size: 6949 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 7954 Fragments arranged by size: 7954 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2649 1186 Size: 1463 6716 BseYI C'CCAG_C Cuts at: 2129 3953 4976 7382 7878 2129 Size: 1824 1023 2406 496 2430 Fragments arranged by size: 2430 2406 1824 1023 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2225 4388 5085 1163 Size: 573 489 2163 697 4257 Fragments arranged by size: 4257 2163 697 573 489 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 5629 6390 7418 1540 Size: 275 186 3628 761 1028 2301 Fragments arranged by size: 3628 2301 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2040 3662 3862 4317 5055 6240 6325 7396 2040 Size: 1622 200 455 738 1185 85 1071 2823 Fragments arranged by size: 2823 1622 1185 1071 738 455 200 85 BsmI GAATG_Cn' Cuts at: 2290 3924 5690 5783 2290 Size: 1634 1766 93 4686 Fragments arranged by size: 4686 1766 1634 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2040 3662 3862 4317 4342 4663 5055 Size: 399 250 1622 200 455 25 321 392 Cuts at: 5055 5498 6240 6325 7396 1391 Size: 443 742 85 1071 2174 Fragments arranged by size: 2174 1622 1071 742 455 443 399 392 321 250 200 85 25 BspEI T'CCGG_A Cuts at: 7842 7842 Size: 8179 BspHI T'CATG_A Cuts at: 2319 2500 2691 6068 7798 2319 Size: 181 191 3377 1730 2700 Fragments arranged by size: 3377 2700 1730 191 181 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3074 4390 5534 844 Size: 2230 1316 1144 3489 Fragments arranged by size: 3489 2230 1316 1144 BsrBI CCG'CTC Cuts at: 1394 1823 6066 1394 Size: 429 4243 3507 Fragments arranged by size: 4243 3507 429 BsrDI GCAATG_nn' Cuts at: 65 1248 6674 6848 65 Size: 1183 5426 174 1396 Fragments arranged by size: 5426 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 6823 7010 1700 Size: 110 5013 187 2869 Fragments arranged by size: 5013 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 4959 96 Size: 4863 3316 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 8179 BssSI C'ACGA_G Cuts at: 6239 7251 6239 Size: 1012 7167 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 8179 BstBI TT'CG_AA Cuts at: 6983 6983 Size: 8179 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 8179 BstXI CCAn_nnnn'nTGG Cuts at: 2981 3937 2981 Size: 956 7223 BstYI r'GATC_y Cuts at: 1972 2228 3418 5509 5876 6261 6278 7730 7816 Size: 256 1190 2091 367 385 17 1452 86 Cuts at: 7816 7828 8174 1972 Size: 12 346 1977 Fragments arranged by size: 2091 1977 1452 1190 385 367 346 256 86 17 12 Bsu36I CC'TnA_GG Cuts at: 3561 4428 3561 Size: 867 7312 BtgI C'CryG_G Cuts at: 513 1065 513 Size: 552 7627 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 7440 Fragments arranged by size: 7440 683 56 BtsI GCAGTG_nn' Cuts at: 2176 3295 5695 6489 6509 2176 Size: 1119 2400 794 20 3846 Fragments arranged by size: 3846 2400 1119 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 8179 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 2724 2759 529 Size: 35 2160 35 5949 Fragments arranged by size: 5949 2160 35 35 DraI TTT'AAA Cuts at: 2328 2511 2859 4408 4615 4732 4947 5483 2328 Size: 183 348 1549 207 117 215 536 5024 Fragments arranged by size: 5024 1549 536 348 215 207 183 117 DraIII CAC_nnn'GTG Cuts at: 3914 3914 Size: 8179 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 7186 809 Size: 507 5870 1802 Fragments arranged by size: 5870 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2907 2926 3520 Size: 54 1425 50 275 18 1077 19 594 Cuts at: 3520 5626 6515 8144 8 Size: 2106 889 1629 43 Fragments arranged by size: 2106 1629 1425 1077 889 594 275 54 50 43 19 18 EagI C'GGCC_G Cuts at: 1537 1812 5626 1537 Size: 275 3814 4090 Fragments arranged by size: 4090 3814 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2013 2136 2294 3936 4169 4692 5316 6109 Size: 660 123 158 1642 233 523 624 793 Cuts at: 6109 1353 Size: 3423 Fragments arranged by size: 3423 1642 793 660 624 523 233 158 123 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 6766 7140 7286 387 Size: 1064 138 5177 374 146 1280 Fragments arranged by size: 5177 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2399 3636 4127 6242 6839 7626 7894 Size: 393 767 1237 491 2115 597 787 268 Cuts at: 7894 1239 Size: 1524 Fragments arranged by size: 2115 1524 1237 787 767 597 491 393 268 EcoNI CCTnn'n_nnAGG Cuts at: 4089 4089 Size: 8179 EcoO109I rG'GnC_Cy Cuts at: 4082 4680 5123 4082 Size: 598 443 7138 Fragments arranged by size: 7138 598 443 EcoRI G'AATT_C Cuts at: 3469 3736 5488 5866 3469 Size: 267 1752 378 5782 Fragments arranged by size: 5782 1752 378 267 EcoRV GAT'ATC Cuts at: 3442 3442 Size: 8179 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 3518 5345 5391 3518 Size: 1827 46 6306 Fragments arranged by size: 6306 1827 46 FspI TGC'GCA Cuts at: 6685 6685 Size: 8179 HaeII r_GCGC'y Cuts at: 1678 7326 1678 Size: 5648 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2108 2140 2431 2463 2478 Size: 32 135 32 536 32 291 32 15 Cuts at: 2478 2510 4122 4154 4394 4426 5270 5302 6820 Size: 32 1612 32 240 32 844 32 1518 Cuts at: 6820 6852 6894 6926 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 1612 1518 844 536 291 240 135 42 32 32 32 32 32 32 32 32 32 32 15 HincII GTy'rAC Cuts at: 669 1657 1938 2116 2365 3969 4073 5523 5769 Size: 988 281 178 249 1604 104 1450 246 Cuts at: 5769 669 Size: 3079 Fragments arranged by size: 3079 1604 1450 988 281 249 246 178 104 HindIII A'AGCT_T Cuts at: 748 2343 4583 4798 5349 748 Size: 1595 2240 215 551 3578 Fragments arranged by size: 3578 2240 1595 551 215 HpaI GTT'AAC Cuts at: 3969 5769 3969 Size: 1800 6379 KpnI G_GTAC'C Cuts at: 5504 5504 Size: 8179 MfeI C'AATT_G Cuts at: 3509 5778 3509 Size: 2269 5910 MluI A'CGCG_T Cuts at: 8055 8055 Size: 8179 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2650 3336 7293 7477 1305 Size: 1345 686 3957 184 2007 Fragments arranged by size: 3957 2007 1345 686 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2909 2928 3522 10 Size: 54 1425 343 1077 19 594 4667 Fragments arranged by size: 4667 1425 1077 594 343 54 19 MslI CAynn'nnrTG Cuts at: 518 1166 2979 3528 3576 3935 4310 6137 6496 Size: 648 1813 549 48 359 375 1827 359 Cuts at: 6496 6655 518 Size: 159 2042 Fragments arranged by size: 2042 1827 1813 648 549 375 359 359 159 48 MspA1I CmG'CkG Cuts at: 1606 3964 4819 5595 6272 7420 7665 1606 Size: 2358 855 776 677 1148 245 2120 Fragments arranged by size: 2358 2120 1148 855 776 677 245 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 7627 NdeI CA'TA_TG Cuts at: 387 3748 5388 387 Size: 3361 1640 3178 Fragments arranged by size: 3361 3178 1640 NheI G'CTAG_C Cuts at: 1052 2350 3017 1052 Size: 1298 667 6214 Fragments arranged by size: 6214 1298 667 NotI GC'GGCC_GC Cuts at: 5626 5626 Size: 8179 NsiI A_TGCA'T Cuts at: 2085 2085 Size: 8179 NspI r_CATG'y Cuts at: 1132 1229 2239 5218 5537 7082 1132 Size: 97 1010 2979 319 1545 2229 Fragments arranged by size: 2979 2229 1545 1010 319 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 8179 PciI A'CATG_T Cuts at: 7078 7078 Size: 8179 PflMI CCAn_nnn'nTGG Cuts at: 1108 3185 3802 5084 5133 1108 Size: 2077 617 1282 49 4154 Fragments arranged by size: 4154 2077 1282 617 49 PmeI GTTT'AAAC Cuts at: 5483 5483 Size: 8179 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 6248 6280 7761 7793 6248 Size: 32 1481 32 6634 Fragments arranged by size: 6634 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 4082 4680 5123 4082 Size: 598 443 7138 Fragments arranged by size: 7138 598 443 PshAI GACnn'nnGTC Cuts at: 1613 4515 1613 Size: 2902 5277 PsiI TTA'TAA Cuts at: 3090 5749 3090 Size: 2659 5520 PstI C_TGCA'G Cuts at: 830 2439 3758 5341 5531 830 Size: 1609 1319 1583 190 3478 Fragments arranged by size: 3478 1609 1583 1319 190 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 8179 PvuII CAG'CTG Cuts at: 3964 4819 3964 Size: 855 7324 SalI G'TCGA_C Cuts at: 1936 5521 1936 Size: 3585 4594 SapI GCTCTTCn'nnn_ Cuts at: 2013 3936 4692 2013 Size: 1923 756 5500 Fragments arranged by size: 5500 1923 756 SbfI CC_TGCA'GG Cuts at: 5531 5531 Size: 8179 ScaI AGT'ACT Cuts at: 3760 4216 4671 6427 8139 3760 Size: 456 455 1756 1712 3800 Fragments arranged by size: 3800 1756 1712 456 455 SfcI C'TryA_G Cuts at: 826 1045 2435 3033 3754 5337 5527 6662 7343 Size: 219 1390 598 721 1583 190 1135 681 Cuts at: 7343 7534 826 Size: 191 1471 Fragments arranged by size: 1583 1471 1390 1135 721 681 598 219 191 190 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 8179 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 8179 SmaI CCC'GGG Cuts at: 1872 5506 1872 Size: 3634 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2474 2844 4201 4318 6283 7184 Size: 197 926 531 370 1357 117 1965 901 Cuts at: 7184 7446 820 Size: 262 1553 Fragments arranged by size: 1965 1553 1357 926 901 531 370 262 197 117 SnaBI TAC'GTA Cuts at: 493 5970 493 Size: 5477 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 8179 SphI G_CATG'C Cuts at: 1132 2239 5537 1132 Size: 1107 3298 3774 Fragments arranged by size: 3774 3298 1107 SspI AAT'ATT Cuts at: 5 52 3254 6103 5 Size: 47 3202 2849 2081 Fragments arranged by size: 3202 2849 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 8179 StyI C'CwwG_G Cuts at: 513 1065 5618 513 Size: 552 4553 3074 Fragments arranged by size: 4553 3074 552 SwaI ATTT'AAAT Cuts at: 4732 4732 Size: 8179 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 5088 6206 6223 6376 6561 7006 325 Size: 1255 3508 1118 17 153 185 445 1498 Fragments arranged by size: 3508 1498 1255 1118 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3230 3568 3635 Size: 274 80 33 51 1443 1253 338 67 Cuts at: 3635 3758 4214 4669 4959 6425 8137 96 Size: 123 456 455 290 1466 1712 138 Fragments arranged by size: 1712 1466 1443 1253 456 455 338 290 274 138 123 80 67 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2516 2548 3403 3435 8139 8171 2516 Size: 32 855 32 4704 32 2524 Fragments arranged by size: 4704 2524 855 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 3192 1316 Size: 1876 6303 XbaI T'CTAG_A Cuts at: 4543 5072 5515 4543 Size: 529 443 7207 Fragments arranged by size: 7207 529 443 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2936 4984 2936 Size: 2048 6131 XhoI C'TCGA_G Cuts at: 1943 2844 1943 Size: 901 7278 XmaI C'CCGG_G Cuts at: 1870 5504 1870 Size: 3634 4545 XmnI GAAnn'nnTTC Cuts at: 6308 6308 Size: 8179 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 5987 276 Size: 53 83 186 936 4453 2468 Fragments arranged by size: 4453 2468 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BaeI BamHI BanI BanII BbsI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI EcoRV FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NsiI NspI PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI PstI PvuI PvuII SalI SapI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI SwaI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI ApaI AscI AvrII BbeI BbvCI BsiWI BsmBI BstZ17I EcoICRI FseI FspAI KasI NaeI NarI NgoMIV NruI PacI PfoI PmlI PspOMI PspXI PsrI RsrII SacI SacII SanDI SexAI SfiI SfoI SrfI