Restriction map for
pFN21AB9101
(Linear) (Six-base) MAPSORT of: KIBB9101.seq Check: 3102 from: 1 to: 3474
KIBB9101.orf
With 182 enzymes: SgfI *
February 28, 2007 11:28 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 2385 3474
Size: 2385 1089
AclI AA'CG_TT
Cuts at: 0 1870 3474
Size: 1870 1604
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 394 3474
Size: 394 3080
AflII C'TTAA_G
Cuts at: 0 2196 3474
Size: 2196 1278
AhdI GACnn_n'nnGTC
Cuts at: 0 117 2131 3474
Size: 117 2014 1343
Fragments arranged by size:
2014 1343 117
AleI CACnn'nnGTG
Cuts at: 0 2305 3474
Size: 2305 1169
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 455 500 3474
Size: 455 45 2974
Fragments arranged by size:
2974 455 45
AlwNI CAG_nnn'CTG
Cuts at: 0 103 1360 2080 3079 3474
Size: 103 1257 720 999 395
Fragments arranged by size:
1257 999 720 395 103
ApaLI G'TGCA_C
Cuts at: 0 31 1853 2308 3474
Size: 31 1822 455 1166
Fragments arranged by size:
1822 1166 455 31
ApoI r'AATT_y
Cuts at: 0 318 1464 1731 1896 2291 2701 2722 2923
Size: 318 1146 267 165 395 410 21 201
Cuts at: 2923 3474
Size: 551
Fragments arranged by size:
1146 551 410 395 318 267 201 165
21
AvaI C'yCGr_G
Cuts at: 0 839 3474
Size: 839 2635
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 0 92 125 3474
Size: 92 33 3349
Fragments arranged by size:
3349 92 33
BamHI G'GATC_C
Cuts at: 0 1413 3474
Size: 1413 2061
BbsI GAAGACnn'nnnn_
Cuts at: 0 257 2402 3474
Size: 257 2145 1072
Fragments arranged by size:
2145 1072 257
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 820 854 3474
Size: 820 34 2620
Fragments arranged by size:
2620 820 34
BfrBI ATG'CAT
Cuts at: 0 78 3474
Size: 78 3396
BlpI GC'TnA_GC
Cuts at: 0 2748 3474
Size: 2748 726
Bme1580I G_kGCm'C
Cuts at: 0 35 1857 2312 2337 2658 3474
Size: 35 1822 455 25 321 816
Fragments arranged by size:
1822 816 455 321 35 25
BmrI ACTGGGnnnn_n'
Cuts at: 0 1622 3101 3474
Size: 1622 1479 373
Fragments arranged by size:
1622 1479 373
BmtI G_CTAG'C
Cuts at: 0 349 1016 3474
Size: 349 667 2458
Fragments arranged by size:
2458 667 349
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 39 3474
Size: 39 3435
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1631 2122 3474
Size: 1631 491 1352
Fragments arranged by size:
1631 1352 491
Bpu10I CC'TnA_GC
Cuts at: 0 1169 3044 3474
Size: 1169 1875 430
Fragments arranged by size:
1875 1169 430
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 454 2334 3474
Size: 454 1880 1140
Fragments arranged by size:
1880 1140 454
BsaI GGTCTCn'nnnn_
Cuts at: 0 1694 1938 3474
Size: 1694 244 1536
Fragments arranged by size:
1694 1536 244
BsaBI GATnn'nnATC
Cuts at: 0 602 1259 3474
Size: 602 657 2215
Fragments arranged by size:
2215 657 602
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 644 3474
Size: 644 2830
BseYI C'CCAG_C
Cuts at: 0 124 1948 2971 3474
Size: 124 1824 1023 503
Fragments arranged by size:
1824 1023 503 124
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 220 2383 3080 3474
Size: 220 2163 697 394
Fragments arranged by size:
2163 697 394 220
BsiHKAI G_wGCw'C
Cuts at: 0 35 1657 1857 2312 3050 3474
Size: 35 1622 200 455 738 424
Fragments arranged by size:
1622 738 455 424 200 35
BsmI GAATG_Cn'
Cuts at: 0 285 1919 3474
Size: 285 1634 1555
Fragments arranged by size:
1634 1555 285
Bsp1286I G_dGCh'C
Cuts at: 0 35 1657 1857 2312 2337 2658 3050 3474
Size: 35 1622 200 455 25 321 392 424
Fragments arranged by size:
1622 455 424 392 321 200 35 25
BspHI T'CATG_A
Cuts at: 0 314 495 686 3474
Size: 314 181 191 2788
Fragments arranged by size:
2788 314 191 181
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 1069 2385 3474
Size: 1069 1316 1089
Fragments arranged by size:
1316 1089 1069
BsrGI T'GTAC_A
Cuts at: 0 2954 3474
Size: 2954 520
BstXI CCAn_nnnn'nTGG
Cuts at: 0 976 1932 3474
Size: 976 956 1542
Fragments arranged by size:
1542 976 956
BstYI r'GATC_y
Cuts at: 0 223 1413 3474
Size: 223 1190 2061
Fragments arranged by size:
2061 1190 223
Bsu36I CC'TnA_GG
Cuts at: 0 1556 2423 3474
Size: 1556 867 1051
Fragments arranged by size:
1556 1051 867
BtsI GCAGTG_nn'
Cuts at: 0 171 1290 3474
Size: 171 1119 2184
Fragments arranged by size:
2184 1119 171
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 719 754 3474
Size: 719 35 2720
Fragments arranged by size:
2720 719 35
DraI TTT'AAA
Cuts at: 0 323 506 854 2403 2610 2727 2942 3474
Size: 323 183 348 1549 207 117 215 532
Fragments arranged by size:
1549 532 348 323 215 207 183 117
DraIII CAC_nnn'GTG
Cuts at: 0 1909 3474
Size: 1909 1565
EaeI y'GGCC_r
Cuts at: 0 902 921 1515 3474
Size: 902 19 594 1959
Fragments arranged by size:
1959 902 594 19
EarI CTCTTCn'nnn_
Cuts at: 0 8 131 289 1931 2164 2687 3311 3474
Size: 8 123 158 1642 233 523 624 163
Fragments arranged by size:
1642 624 523 233 163 158 123 8
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 394 1631 2122 3474
Size: 394 1237 491 1352
Fragments arranged by size:
1352 1237 491 394
EcoNI CCTnn'n_nnAGG
Cuts at: 0 2084 3474
Size: 2084 1390
EcoO109I rG'GnC_Cy
Cuts at: 0 2077 2675 3118 3474
Size: 2077 598 443 356
Fragments arranged by size:
2077 598 443 356
EcoRI G'AATT_C
Cuts at: 0 1464 1731 3474
Size: 1464 267 1743
Fragments arranged by size:
1743 1464 267
EcoRV GAT'ATC
Cuts at: 0 1437 3474
Size: 1437 2037
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 1513 3340 3386 3474
Size: 1513 1827 46 88
Fragments arranged by size:
1827 1513 88 46
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 103 135 426 458 473 505 2117 2149
Size: 103 32 291 32 15 32 1612 32
Cuts at: 2149 2389 2421 3265 3297 3474
Size: 240 32 844 32 177
Fragments arranged by size:
1612 844 291 240 177 103 32 32
32 32 32 32 15
HincII GTy'rAC
Cuts at: 0 111 360 1964 2068 3474
Size: 111 249 1604 104 1406
Fragments arranged by size:
1604 1406 249 111 104
HindIII A'AGCT_T
Cuts at: 0 338 2578 2793 3344 3474
Size: 338 2240 215 551 130
Fragments arranged by size:
2240 551 338 215 130
HpaI GTT'AAC
Cuts at: 0 1964 3474
Size: 1964 1510
MfeI C'AATT_G
Cuts at: 0 1504 3474
Size: 1504 1970
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 645 1331 3474
Size: 645 686 2143
Fragments arranged by size:
2143 686 645
MscI TGG'CCA
Cuts at: 0 904 923 1517 3474
Size: 904 19 594 1957
Fragments arranged by size:
1957 904 594 19
MslI CAynn'nnrTG
Cuts at: 0 974 1523 1571 1930 2305 3474
Size: 974 549 48 359 375 1169
Fragments arranged by size:
1169 974 549 375 359 48
MspA1I CmG'CkG
Cuts at: 0 1959 2814 3474
Size: 1959 855 660
Fragments arranged by size:
1959 855 660
NdeI CA'TA_TG
Cuts at: 0 1743 3383 3474
Size: 1743 1640 91
Fragments arranged by size:
1743 1640 91
NheI G'CTAG_C
Cuts at: 0 345 1012 3474
Size: 345 667 2462
Fragments arranged by size:
2462 667 345
NsiI A_TGCA'T
Cuts at: 0 80 3474
Size: 80 3394
NspI r_CATG'y
Cuts at: 0 234 3213 3474
Size: 234 2979 261
Fragments arranged by size:
2979 261 234
PflMI CCAn_nnn'nTGG
Cuts at: 0 1180 1797 3079 3128 3474
Size: 1180 617 1282 49 346
Fragments arranged by size:
1282 1180 617 346 49
PpuMI rG'GwC_Cy
Cuts at: 0 2077 2675 3118 3474
Size: 2077 598 443 356
Fragments arranged by size:
2077 598 443 356
PshAI GACnn'nnGTC
Cuts at: 0 2510 3474
Size: 2510 964
PsiI TTA'TAA
Cuts at: 0 1085 3474
Size: 1085 2389
PstI C_TGCA'G
Cuts at: 0 434 1753 3336 3474
Size: 434 1319 1583 138
Fragments arranged by size:
1583 1319 434 138
PvuII CAG'CTG
Cuts at: 0 1959 2814 3474
Size: 1959 855 660
Fragments arranged by size:
1959 855 660
SapI GCTCTTCn'nnn_
Cuts at: 0 8 1931 2687 3474
Size: 8 1923 756 787
Fragments arranged by size:
1923 787 756 8
ScaI AGT'ACT
Cuts at: 0 1755 2211 2666 3474
Size: 1755 456 455 808
Fragments arranged by size:
1755 808 456 455
SfcI C'TryA_G
Cuts at: 0 430 1028 1749 3332 3474
Size: 430 598 721 1583 142
Fragments arranged by size:
1583 721 598 430 142
SmlI C'TyrA_G
Cuts at: 0 469 839 2196 2313 3474
Size: 469 370 1357 117 1161
Fragments arranged by size:
1357 1161 469 370 117
SphI G_CATG'C
Cuts at: 0 234 3474
Size: 234 3240
SspI AAT'ATT
Cuts at: 0 1249 3474
Size: 1249 2225
SwaI ATTT'AAAT
Cuts at: 0 2727 3474
Size: 2727 747
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 3083 3474
Size: 3083 391
TatI w'GTAC_w
Cuts at: 0 1225 1563 1630 1753 2209 2664 2954 3474
Size: 1225 338 67 123 456 455 290 520
Fragments arranged by size:
1225 520 456 455 338 290 123 67
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 511 543 1398 1430 3474
Size: 511 32 855 32 2044
Fragments arranged by size:
2044 855 511 32 32
Tth111I GACn'n_nGTC
Cuts at: 0 1187 3474
Size: 1187 2287
XbaI T'CTAG_A
Cuts at: 0 2538 3067 3474
Size: 2538 529 407
Fragments arranged by size:
2538 529 407
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 931 2979 3474
Size: 931 2048 495
Fragments arranged by size:
2048 931 495
XhoI C'TCGA_G
Cuts at: 0 839 3474
Size: 839 2635
Enzymes that do cut:
AarI AclI AcuI AflII AhdI AleI AlfI AlwNI
ApaLI ApoI AvaI BaeI BamHI BbsI BcgI BfrBI
BlpI Bme1580I BmrI BmtI BplI BpmI Bpu10I BpuEI
BsaI BsaBI BseRI BseYI BsgI BsiHKAI BsmI Bsp1286I
BspHI BspMI BsrGI BstXI BstYI Bsu36I BtsI CspCI
DraI DraIII EaeI EarI Eco57MI EcoNI EcoO109I EcoRI
EcoRV FalI Hin4I HincII HindIII HpaI MfeI MmeI
MscI MslI MspA1I NdeI NheI NsiI NspI PflMI
PpuMI PshAI PsiI PstI PvuII SapI ScaI SfcI
SmlI SphI SspI SwaI TaqII TatI TstI Tth111I
XbaI XcmI XhoI
Enzymes that do not cut:
AatII AccI Acc65I AfeI AflIII AgeI AloI ApaI
AscI AseI AsiSI AvrII BanI BanII BbeI BbvCI
BciVI BclI BglI BglII BmgBI BsaAI BsaHI BsaWI
BsaXI BsiEI BsiWI BsmBI BspEI BsrBI BsrDI BsrFI
BssHII BssSI BstAPI BstBI BstEII BstZ17I BtgI BtgZI
ClaI DrdI EagI EciI EcoICRI FseI FspI FspAI
HaeII KasI KpnI MluI NaeI NarI NcoI NgoMIV
NotI NruI PacI PasI PciI PfoI PmeI PmlI
PpiI PspOMI PspXI PsrI PvuI RsrII SacI SacII
SalI SanDI SbfI SexAI SfiI SfoI SgfI SgrAI
SmaI SnaBI SpeI SrfI StuI StyI XmaI XmnI
ZraI