Restriction map for
pFN21AB9025
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# NO CUT ENZYMES ( 159 enzymes) #
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AatII Acc65I AccIII AclI AcyI
AflII AflIII AgeI AhdI AluI
Alw44I AlwNI Aor51HI ApaI ApaLI
AscI AseI AsnI Asp700I Asp718I
AspEI AvaI AviII AvrII BamHI
BbeI BbrPI BcgI BciVI BclI
BcnI BfrI BglI BlnI BlpI
BpmI Bpu1102I BsaAI BsaBI BsaHI
BsaI BsaWI BseAI BsgI BsiEI
BsiWI BsmBI BsmFI BsmI BsoBI
Bsp1407I BspDI BspEI BspLU11I BsrBI
BsrDI BsrFI BsrGI BssHII Bst1107I
BstBI BstXI BstZ17I Cac8I CelII
CfoI Cfr10I ClaI CpoI DraII
DraIII DsaI EagI Eam1105I EclXI
Eco47III Eco52I EcoO109I EcoRV EcoT22I
FauI FbaI FseI FspI HaeII
HapII HgaI HhaI Hin1I HinP1I
HincII HindII HindIII HpaI HpaII
KasI KpnI KspI MaeII MamI
MluI MroI MslI MspA1I MspI
NaeI NarI NciI NcoI NdeI
NgoMIV NheI NotI NsiI NspV
PaeR7I PflMI PinAI PmaCI PmeI
PmlI PpuMI PshAI PshBI Psp1406I
PstI PvuI PvuII RsrII SacI
SacII SalI SapI SbfI ScaI
SfcI SfiI SfoI SfuI SgrAI
SmaI SmlI SnaBI SpeI SphI
Sse8387I SspBI SspI StuI SwaI
TaqI TthHB8I Van91I XbaI XcmI
XhoI XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 51 enzymes) #
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AccI 625 GTAGAC GT^[AC][GT]AC
AccII 945 CGCG CG^CG
AciI 442 GCGG CCGC(-3/-1)
AlwI 646 GATCC GGATC(4/5)
AocI 223 CCTCAGG CC^T[ACGT]AGG
AspHI 1111 GAGCAC G[AT]GC[AT]^C
AspI 835 GACAAAGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvaII 1142 GGTCC G^G[AT]CC
BalI 1167 TGGCCA TGG^CCA
BanI 8 GGTGCC G^G[CT][GA]CC
BanII 1214 GGGCTC G[GA]GC[CT]^C
BbvI 1134 GCAGC GCAGC(8/12)
BfaI 1058 CTAG C^TAG
BglII 453 AGATCT A^GATCT
BsiHKAI 1111 GAGCAC G[AT]GC[AT]^C
BspHI 345 TCATGA T^CATGA
BspMI 807 ACCTGC ACCTGC(4/8)
BssSI 1079 CTCGTG CACGAG(-5/-1)
BstAPI 670 GCAGAAAATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstF5I 296 GGATG GGATG(2/0)
BstUI 945 CGCG CG^CG
BstYI 453 AGATCT [GA]^GATC[CT]
Bsu36I 223 CCTCAGG CC^T[ACGT]AGG
Cfr13I 1142 GGTCC G^G[ACGT]CC
DraI 525 TTTAAA TTT^AAA
DrdI 131 GACTGAAGAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EaeI 1167 TGGCCA [CT]^GGCC[GA]
EarI 135 GAAGAG CTCTTC(1/4)
Eco81I 223 CCTCAGG CC^T[ACGT]AGG
EcoNI 793 CCTTATATAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoRI 106 GAATTC G^AATTC
Fnu4HI 1134 GCAGC GC^[ACGT]GC
FokI 296 GGATG GGATG(9/13)
ItaI 1134 GCAGC GC^[ACGT]GC
Ksp632I 135 GAAGAG CTCTTC(1/4)
MaeI 1058 CTAG C^TAG
MfeI 665 CAATTG C^AATTG
MflI 453 AGATCT [GA]^GATC[CT]
MluNI 1167 TGGCCA TGG^CCA
MscI 1167 TGGCCA TGG^CCA
MunI 665 CAATTG C^AATTG
MvnI 945 CGCG CG^CG
NruI 944 TCGCGA TCG^CGA
PacI 997 TTAATTAA TTAAT^TAA
PflFI 835 GACAAAGTC GAC[ACGT]^[ACGT][ACGT]GTC
RcaI 345 TCATGA T^CATGA
Sau96I 1142 GGTCC G^G[ACGT]CC
SexAI 633 ACCTGGT A^CC[AT]GGT
TseI 1134 GCAGC G^C[AT]GC
Tth111I 835 GACAAAGTC GAC[ACGT]^[ACGT][ACGT]GTC
XhoII 453 AGATCT [GA]^GATC[CT]
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# TWO CUT ENZYMES ( 20 enzymes) #
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AfaI 904 GTAC GT^AC
1101 GTAC
BbsI 398 GAAGAC GAAGAC(2/6)
1296 GAAGAC
BbvCI 3 GCTGAGG CCTCAGC(-5/-2)
406 GCTGAGG
BmyI 1111 GAGCAC G[AGT]GC[ACT]^C
1214 GGGCTC
BpuAI 398 GAAGAC GAAGAC(2/6)
1296 GAAGAC
BsaJI 963 CCTTGG C^C[ACGT][ACGT]GG
1104 CCAAGG
BsiYI 793 CCTTATATAGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
1159 CCTGTAAGTGG
BslI 793 CCTTATATAGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
1159 CCTGTAAGTGG
Bsp1286I 1111 GAGCAC G[AGT]GC[ACT]^C
1214 GGGCTC
BssKI 338 CCAGG ^CC[ACGT]GG
634 CCTGG
BstNI 338 CCAGG CC^[AT]GG
634 CCTGG
EcoRII 338 CCAGG ^CC[AT]GG
634 CCTGG
EcoT14I 963 CCTTGG C^C[AT][AT]GG
1104 CCAAGG
MvaI 338 CCAGG CC^[AT]GG
634 CCTGG
NspI 402 ACATGC [GA]CATG^[CT]
555 ACATGC
PleI 879 GACTC GAGTC(4/5)
138 GAGTC
RsaI 904 GTAC GT^AC
1101 GTAC
ScrFI 338 CCAGG CC^[ACGT]GG
634 CCTGG
SfaNI 439 GATGC GCATC(5/9)
1069 GATGC
StyI 963 CCTTGG C^C[AT][AT]GG
1104 CCAAGG
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# 3 OR MORE CUT ENZYMES ( 28 enzymes) #
#########################################
AcsI ApoI BseRI BsmAI BsrI
BstEII BstPI DdeI DpnII EcoO65I
HaeIII HinfI HphI MaeIII MboI
MboII MnlI MseI MwoI NdeII
NlaIII NlaIV Sau3AI TfiI Tru9I
Tsp45I Tsp509I TspRI