Restriction map for
pFN21AB9025
(Circular) (Six-base) MAPSORT of: pFN21AB9025.seq Check: 8986 from: 1 to: 6007
pFN21AB9025 6007 bp
With 182 enzymes: SgfI *
January 16, 2009 15:28 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3817 278
Size: 53 83 186 936 2281 2468
Fragments arranged by size:
2468 2281 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2631 3350 1937
Size: 694 719 4594
Fragments arranged by size:
4594 719 694
Acc65I G'GTAC_C
Cuts at: 3328 3328
Size: 6007
AclI AA'CG_TT
Cuts at: 1563 4134 4507 1563
Size: 2571 373 3063
Fragments arranged by size:
3063 2571 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2093 2159 2593 3122 3186 4070 5454 5722
Size: 461 66 434 529 64 884 1384 268
Cuts at: 5722 1632
Size: 1917
Fragments arranged by size:
1917 1384 884 529 461 434 268 66
64
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5810
AflIII A'CryG_T
Cuts at: 1203 4906 5883 1203
Size: 3703 977 1327
Fragments arranged by size:
3703 1327 977
AgeI A'CCGG_T
Cuts at: 4838 4838
Size: 6007
AhdI GACnn_n'nnGTC
Cuts at: 4736 4736
Size: 6007
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6007
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2460 1184
Size: 1276 4731
AlwNI CAG_nnn'CTG
Cuts at: 3750 5322 3750
Size: 1572 4435
ApaLI G'TGCA_C
Cuts at: 4064 5220 4064
Size: 1156 4851
ApoI r'AATT_y
Cuts at: 1493 2111 2359 2420 2470 2595 2736 2834 3316
Size: 618 248 61 50 125 141 98 482
Cuts at: 3316 3542 3694 5803 1493
Size: 226 152 2109 1697
Fragments arranged by size:
2109 1697 618 482 248 226 152 141
125 98 61 50
AseI AT'TA_AT
Cuts at: 160 1032 4561 160
Size: 872 3529 1606
Fragments arranged by size:
3529 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6007
AvaI C'yCGr_G
Cuts at: 1870 1943 3332 1870
Size: 73 1389 4545
Fragments arranged by size:
4545 1389 73
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 3089 3122 3089
Size: 33 5974
BamHI G'GATC_C
Cuts at: 3337 3704 3337
Size: 367 5640
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2013 3328 4783 618
Size: 325 217 627 226 1315 1455 1842
Fragments arranged by size:
1842 1455 1315 627 325 226 217
BanII G_rGCy'C
Cuts at: 1391 3223 3326 1391
Size: 1832 103 4072
Fragments arranged by size:
4072 1832 103
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2410 3308 928
Size: 971 511 898 3627
Fragments arranged by size:
3627 971 898 511
BbvCI CC'TCA_GC
Cuts at: 2009 2412 2009
Size: 403 5604
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 1987 2021 4198 4232 1903
Size: 34 50 34 2177 34 3678
Fragments arranged by size:
3678 2177 50 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3899 5109 3899
Size: 1210 4797
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6007
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4618 136
Size: 107 122 71 631 605 2946 1525
Fragments arranged by size:
2946 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2458 6002 2458
Size: 3544 2463
BlpI GC'TnA_GC
Cuts at: 3424 3424
Size: 6007
Bme1580I G_kGCm'C
Cuts at: 1790 4068 5224 1790
Size: 2278 1156 2573
Fragments arranged by size:
2573 2278 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6007
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3738 4696 442
Size: 416 53 83 399 24 2321 958 1753
Fragments arranged by size:
2321 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6007
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 4667 1239
Size: 3428 2579
Bpu10I CC'TnA_GC
Cuts at: 2009 2412 2009
Size: 403 5604
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4132 4997 5295 4132
Size: 865 298 4844
Fragments arranged by size:
4844 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4670 882
Size: 617 3171 2219
Fragments arranged by size:
3171 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3798 493
Size: 711 2594 2702
Fragments arranged by size:
2702 2594 711
BsaBI GATnn'nnATC
Cuts at: 6001 6001
Size: 6007
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3814 4196 4831 275
Size: 53 83 186 936 2281 382 635 1451
Fragments arranged by size:
2281 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4440 4838 5112 5259 5670 4440
Size: 398 274 147 411 4777
Fragments arranged by size:
4777 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2184 2214 1178
Size: 30 165 30 781 30 4971
Fragments arranged by size:
4971 781 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2208 2331 2972 1186
Size: 1022 123 641 4221
Fragments arranged by size:
4221 1022 641 123
BseYI C'CCAG_C
Cuts at: 2291 5210 5706 2291
Size: 2919 496 2592
Fragments arranged by size:
2919 2592 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5434
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3457 4218 5246 1540
Size: 275 186 1456 761 1028 2301
Fragments arranged by size:
2301 1456 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 3120 4068 4153 5224 3120
Size: 948 85 1071 3903
Fragments arranged by size:
3903 1071 948 85
BsmI GAATG_Cn'
Cuts at: 3518 3611 3518
Size: 93 5914
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 3120 3223 3326 4068 4153 5224 1391
Size: 399 1330 103 103 742 85 1071 2174
Fragments arranged by size:
2174 1330 1071 742 399 103 103 85
BspEI T'CCGG_A
Cuts at: 5670 5670
Size: 6007
BspHI T'CATG_A
Cuts at: 2350 3896 5626 2350
Size: 1546 1730 2731
Fragments arranged by size:
2731 1730 1546
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2821 3362 844
Size: 1977 541 3489
Fragments arranged by size:
3489 1977 541
BsrBI CCG'CTC
Cuts at: 1394 1823 3894 1394
Size: 429 2071 3507
Fragments arranged by size:
3507 2071 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4502 4676 65
Size: 1183 3254 174 1396
Fragments arranged by size:
3254 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4651 4838 1700
Size: 110 2841 187 2869
Fragments arranged by size:
2869 2841 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6007
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6007
BssSI C'ACGA_G
Cuts at: 3084 4067 5079 3084
Size: 983 1012 4012
Fragments arranged by size:
4012 1012 983
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2681 794
Size: 1887 4120
BstBI TT'CG_AA
Cuts at: 4811 4811
Size: 6007
BstEII G'GTnAC_C
Cuts at: 1184 2642 2747 3260 1184
Size: 1458 105 513 3931
Fragments arranged by size:
3931 1458 513 105
BstYI r'GATC_y
Cuts at: 1972 2458 3337 3704 4089 4106 5558 5644 5656
Size: 486 879 367 385 17 1452 86 12
Cuts at: 5656 6002 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 879 486 385 367 346 86
17 12
Bsu36I CC'TnA_GG
Cuts at: 2229 2229
Size: 6007
BtgI C'CryG_G
Cuts at: 513 1065 513
Size: 552 5455
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5268
Fragments arranged by size:
5268 683 56
BtsI GCAGTG_nn'
Cuts at: 2205 2250 3149 3523 4317 4337 2205
Size: 45 899 374 794 20 3875
Fragments arranged by size:
3875 899 794 374 45 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6007
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5972
DraI TTT'AAA
Cuts at: 2532 3311 2532
Size: 779 5228
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2142 5014 809
Size: 507 826 2872 1802
Fragments arranged by size:
2872 1802 826 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3172 3454 4343
Size: 54 1425 50 275 18 1342 282 889
Cuts at: 4343 5972 8
Size: 1629 43
Fragments arranged by size:
1629 1425 1342 889 282 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3454 1537
Size: 275 1642 4090
Fragments arranged by size:
4090 1642 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2135 3937 1353
Size: 782 1802 3423
Fragments arranged by size:
3423 1802 782
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4594 4968 5114 387
Size: 1064 138 3005 374 146 1280
Fragments arranged by size:
3005 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2093 2159 2593 3122 3186 4070 4667
Size: 393 461 66 434 529 64 884 597
Cuts at: 4667 5454 5722 1239
Size: 787 268 1524
Fragments arranged by size:
1524 884 787 597 529 461 434 393
268 66 64
EcoNI CCTnn'n_nnAGG
Cuts at: 2802 2802
Size: 6007
EcoRI G'AATT_C
Cuts at: 2111 3316 3694 2111
Size: 1205 378 4424
Fragments arranged by size:
4424 1205 378
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3299 3299
Size: 6007
FspI TGC'GCA
Cuts at: 4513 4513
Size: 6007
HaeII r_GCGC'y
Cuts at: 1678 5154 1678
Size: 3476 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 4648 4680 4722 4754 1373
Size: 32 135 32 3076 32 42 32 2626
Fragments arranged by size:
3076 2626 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3351 3597 669
Size: 988 281 1413 246 3079
Fragments arranged by size:
3079 1413 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6007
HpaI GTT'AAC
Cuts at: 3597 3597
Size: 6007
KpnI G_GTAC'C
Cuts at: 3332 3332
Size: 6007
MfeI C'AATT_G
Cuts at: 2670 3606 2670
Size: 936 5071
MluI A'CGCG_T
Cuts at: 5883 5883
Size: 6007
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2536 5121 5305 1305
Size: 1231 2585 184 2007
Fragments arranged by size:
2585 2007 1231 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 3174 10
Size: 54 1425 343 1342 2843
Fragments arranged by size:
2843 1425 1342 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3965 4324 4483 518
Size: 648 2799 359 159 2042
Fragments arranged by size:
2799 2042 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 3423 4100 5248 5493 1606
Size: 1817 677 1148 245 2120
Fragments arranged by size:
2120 1817 1148 677 245
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 5455
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6007
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6007
NotI GC'GGCC_GC
Cuts at: 3454 3454
Size: 6007
NruI TCG'CGA
Cuts at: 2951 2951
Size: 6007
NspI r_CATG'y
Cuts at: 1132 1229 2411 2564 3365 4910 1132
Size: 97 1182 153 801 1545 2229
Fragments arranged by size:
2229 1545 1182 801 153 97
PacI TTA_AT'TAA
Cuts at: 3006 3006
Size: 6007
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6007
PciI A'CATG_T
Cuts at: 4906 4906
Size: 6007
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 6007
PmeI GTTT'AAAC
Cuts at: 3311 3311
Size: 6007
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4076 4108 5589 5621 4076
Size: 32 1481 32 4462
Fragments arranged by size:
4462 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6007
PsiI TTA'TAA
Cuts at: 3577 3577
Size: 6007
PstI C_TGCA'G
Cuts at: 830 3359 830
Size: 2529 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6007
SalI G'TCGA_C
Cuts at: 1936 3349 1936
Size: 1413 4594
SbfI CC_TGCA'GG
Cuts at: 3359 3359
Size: 6007
ScaI AGT'ACT
Cuts at: 4255 5967 4255
Size: 1712 4295
SexAI A'CCwGG_T
Cuts at: 2638 2638
Size: 6007
SfcI C'TryA_G
Cuts at: 826 1045 3355 4490 5171 5362 826
Size: 219 2310 1135 681 191 1471
Fragments arranged by size:
2310 1471 1135 681 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6007
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6007
SmaI CCC'GGG
Cuts at: 1872 3334 1872
Size: 1462 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 4111 5012 5274 820
Size: 197 926 2168 901 262 1553
Fragments arranged by size:
2168 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3798 493
Size: 3305 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6007
SphI G_CATG'C
Cuts at: 1132 3365 1132
Size: 2233 3774
SspI AAT'ATT
Cuts at: 5 52 3931 5
Size: 47 3879 2081
Fragments arranged by size:
3879 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6007
StyI C'CwwG_G
Cuts at: 513 1065 2968 3109 3446 513
Size: 552 1903 141 337 3074
Fragments arranged by size:
3074 1903 552 337 141
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2710 4034 4051 4204 4389 4834 325
Size: 1255 1130 1324 17 153 185 445 1498
Fragments arranged by size:
1498 1324 1255 1130 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2908 4253 5965
Size: 274 80 33 51 1443 931 1345 1712
Cuts at: 5965 96
Size: 138
Fragments arranged by size:
1712 1443 1345 931 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2184 2216 3241 3273 5967 5999 2184
Size: 32 1025 32 2694 32 2192
Fragments arranged by size:
2694 2192 1025 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 2843 1316
Size: 1527 4480
XbaI T'CTAG_A
Cuts at: 3343 3343
Size: 6007
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6007
XmaI C'CCGG_G
Cuts at: 1870 3332 1870
Size: 1462 4545
XmnI GAAnn'nnTTC
Cuts at: 4136 4136
Size: 6007
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3815 276
Size: 53 83 186 936 2281 2468
Fragments arranged by size:
2468 2281 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BaeI BamHI BanI BanII BbsI BbvCI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstYI Bsu36I BtgI
BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI
EarI EciI Eco57MI EcoNI EcoRI FalI FspI HaeII
Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI
MscI MslI MspA1I NcoI NdeI NheI NotI NruI
NspI PacI PasI PciI PflMI PmeI PpiI PshAI
PsiI PstI PvuI SalI SbfI ScaI SexAI SfcI
SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI
StuI StyI TaqII TatI TstI Tth111I XbaI XhoI
XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BbeI BfrBI
BplI BsiWI BsmBI BstXI BstZ17I DraIII EcoICRI EcoO109I
EcoRV FseI FspAI KasI NaeI NarI NgoMIV NsiI
PfoI PmlI PpuMI PspOMI PspXI PsrI PvuII RsrII
SacI SacII SanDI SapI SfiI SfoI SrfI SwaI
XcmI