(Linear) (Six-base) MAPSORT of: KIBB9025.seq  Check: 205  from: 1  to: 1302



KIBB9025.orf

 With 182 enzymes: SgfI * 

                        February 28, 2007 11:28  ..

AccI GT'mk_AC

Cuts at:      0     626    1302 
   Size:        626     676
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      88     154     588    1117    1181    1302 
   Size:         88      66     434     529      64     121

  Fragments arranged by size: 

                 529     434     121      88      66      64
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     455    1302 
   Size:        455     847
 
ApoI r'AATT_y

Cuts at:      0     106     354     415     465     590     731     829    1302 
   Size:        106     248      61      50     125     141      98     473

  Fragments arranged by size: 

                 473     248     141     125     106      98      61      50
            
 
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'

Cuts at:      0    1084    1117    1302 
   Size:       1084      33     185

  Fragments arranged by size: 

                1084     185      33
 
BanI G'GyrC_C

Cuts at:      0       8    1302 
   Size:          8    1294
 
BanII G_rGCy'C

Cuts at:      0    1218    1302 
   Size:       1218      84
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     405    1302 
   Size:        405     897
 
BbvCI CC'TCA_GC

Cuts at:      0       4     407    1302 
   Size:          4     403     895

  Fragments arranged by size: 

                 895     403       4
 
BglII A'GATC_T

Cuts at:      0     453    1302 
   Size:        453     849
 
Bpu10I CC'TnA_GC

Cuts at:      0       4     407    1302 
   Size:          4     403     895

  Fragments arranged by size: 

                 895     403       4
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     179     209    1302 
   Size:        179      30    1093

  Fragments arranged by size: 

                1093     179      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     203     326     967    1302 
   Size:        203     123     641     335

  Fragments arranged by size: 

                 641     335     203     123
 
BseYI C'CCAG_C

Cuts at:      0     286    1302 
   Size:        286    1016
 
BsiHKAI G_wGCw'C

Cuts at:      0    1115    1302 
   Size:       1115     187
 
Bsp1286I G_dGCh'C

Cuts at:      0    1115    1218    1302 
   Size:       1115     103      84

  Fragments arranged by size: 

                1115     103      84
 
BspHI T'CATG_A

Cuts at:      0     345    1302 
   Size:        345     957
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     816    1302 
   Size:        816     486
 
BssSI C'ACGA_G

Cuts at:      0    1079    1302 
   Size:       1079     223
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0     676    1302 
   Size:        676     626
 
BstEII G'GTnAC_C

Cuts at:      0     637     742    1255    1302 
   Size:        637     105     513      47

  Fragments arranged by size: 

                 637     513     105      47
 
BstYI r'GATC_y

Cuts at:      0     453    1302 
   Size:        453     849
 
Bsu36I CC'TnA_GG

Cuts at:      0     224    1302 
   Size:        224    1078
 
BtsI GCAGTG_nn'

Cuts at:      0     200     245    1144    1302 
   Size:        200      45     899     158

  Fragments arranged by size: 

                 899     200     158      45
 
DraI TTT'AAA

Cuts at:      0     527    1302 
   Size:        527     775
 
DrdI GACnn_nn'nnGTC

Cuts at:      0     137    1302 
   Size:        137    1165
 
EaeI y'GGCC_r

Cuts at:      0    1167    1302 
   Size:       1167     135
 
EarI CTCTTCn'nnn_

Cuts at:      0     130    1302 
   Size:        130    1172
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      88     154     588    1117    1181    1302 
   Size:         88      66     434     529      64     121

  Fragments arranged by size: 

                 529     434     121      88      66      64
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0     797    1302 
   Size:        797     505
 
EcoRI G'AATT_C

Cuts at:      0     106    1302 
   Size:        106    1196
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0    1294    1302 
   Size:       1294       8
 
MfeI C'AATT_G

Cuts at:      0     665    1302 
   Size:        665     637
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     531    1302 
   Size:        531     771
 
MscI TGG'CCA

Cuts at:      0    1169    1302 
   Size:       1169     133
 
NruI TCG'CGA

Cuts at:      0     946    1302 
   Size:        946     356
 
NspI r_CATG'y

Cuts at:      0     406     559    1302 
   Size:        406     153     743

  Fragments arranged by size: 

                 743     406     153
 
PacI TTA_AT'TAA

Cuts at:      0    1001    1302 
   Size:       1001     301
 
SexAI A'CCwGG_T

Cuts at:      0     633    1302 
   Size:        633     669
 
StyI C'CwwG_G

Cuts at:      0     963    1104    1302 
   Size:        963     141     198

  Fragments arranged by size: 

                 963     198     141
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     705    1302 
   Size:        705     597
 
TatI w'GTAC_w

Cuts at:      0     903    1302 
   Size:        903     399
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     179     211    1236    1268    1302 
   Size:        179      32    1025      32      34

  Fragments arranged by size: 

                1025     179      34      32      32
 
Tth111I GACn'n_nGTC

Cuts at:      0     838    1302 
   Size:        838     464
 

 Enzymes that do cut:

     AccI     AcuI     AlfI     ApoI     BaeI     BanI    BanII     BbsI
    BbvCI    BglII   Bpu10I    BsaXI    BseRI    BseYI  BsiHKAI Bsp1286I
    BspHI    BspMI    BssSI   BstAPI   BstEII    BstYI   Bsu36I     BtsI
     DraI     DrdI     EaeI     EarI  Eco57MI    EcoNI    EcoRI     FalI
     MfeI     MmeI     MscI     NruI     NspI     PacI    SexAI     StyI
    TaqII     TatI     TstI  Tth111I

 Enzymes that do not cut: 

     AarI    AatII   Acc65I     AclI     AfeI    AflII   AflIII     AgeI
     AhdI     AleI     AloI    AlwNI     ApaI    ApaLI     AscI     AseI
    AsiSI     AvaI    AvrII    BamHI     BbeI     BcgI    BciVI     BclI
    BfrBI     BglI     BlpI Bme1580I    BmgBI     BmrI     BmtI     BplI
     BpmI    BpuEI     BsaI    BsaAI    BsaBI    BsaHI    BsaWI     BsgI
    BsiEI    BsiWI     BsmI    BsmBI    BspEI    BsrBI    BsrDI    BsrFI
    BsrGI   BssHII    BstBI    BstXI  BstZ17I     BtgI    BtgZI     ClaI
    CspCI   DraIII     EagI     EciI  EcoICRI EcoO109I    EcoRV     FseI
     FspI    FspAI    HaeII    Hin4I   HincII  HindIII     HpaI     KasI
     KpnI     MluI     MslI   MspA1I     NaeI     NarI     NcoI     NdeI
   NgoMIV     NheI     NotI     NsiI     PasI     PciI    PflMI     PfoI
     PmeI     PmlI     PpiI    PpuMI    PshAI     PsiI   PspOMI    PspXI
     PsrI     PstI     PvuI    PvuII    RsrII     SacI    SacII     SalI
    SanDI     SapI     SbfI     ScaI     SfcI     SfiI     SfoI     SgfI
    SgrAI     SmaI     SmlI    SnaBI     SpeI     SphI     SrfI     SspI
     StuI     SwaI     XbaI     XcmI     XhoI     XmaI     XmnI     ZraI