(Circular) (Six-base) MAPSORT of: pFN21AB9017.seq Check: 6893 from: 1 to: 6034 pFN21AB9017 6034 bp With 182 enzymes: SgfI * October 20, 2009 10:41 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3844 278 Size: 53 83 186 936 2308 2468 Fragments arranged by size: 2468 2308 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3079 3377 1937 Size: 1142 298 4594 Fragments arranged by size: 4594 1142 298 Acc65I G'GTAC_C Cuts at: 3355 3355 Size: 6034 AclI AA'CG_TT Cuts at: 1563 4161 4534 1563 Size: 2598 373 3063 Fragments arranged by size: 3063 2598 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2184 2633 4097 5481 5749 1632 Size: 552 449 1464 1384 268 1917 Fragments arranged by size: 1917 1464 1384 552 449 268 AflII C'TTAA_G Cuts at: 820 1017 3235 820 Size: 197 2218 3619 Fragments arranged by size: 3619 2218 197 AflIII A'CryG_T Cuts at: 1203 4933 5910 1203 Size: 3730 977 1327 Fragments arranged by size: 3730 1327 977 AgeI A'CCGG_T Cuts at: 4865 4865 Size: 6034 AhdI GACnn_n'nnGTC Cuts at: 4763 4763 Size: 6034 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6034 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6034 AlwNI CAG_nnn'CTG Cuts at: 2752 3777 5349 2752 Size: 1025 1572 3437 Fragments arranged by size: 3437 1572 1025 ApaLI G'TGCA_C Cuts at: 2311 3087 4091 5247 2311 Size: 776 1004 1156 3098 Fragments arranged by size: 3098 1156 1004 776 ApoI r'AATT_y Cuts at: 1493 2537 2807 2831 3070 3343 3569 3721 5830 Size: 1044 270 24 239 273 226 152 2109 Cuts at: 5830 1493 Size: 1697 Fragments arranged by size: 2109 1697 1044 273 270 239 226 152 24 AseI AT'TA_AT Cuts at: 160 1032 2693 2717 4588 160 Size: 872 1661 24 1871 1606 Fragments arranged by size: 1871 1661 1606 872 24 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6034 AvaI C'yCGr_G Cuts at: 1870 1943 2145 2490 2517 3359 1870 Size: 73 202 345 27 842 4545 Fragments arranged by size: 4545 842 345 202 73 27 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 2714 2747 2714 Size: 33 6001 BamHI G'GATC_C Cuts at: 3364 3731 3364 Size: 367 5667 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3355 4810 618 Size: 325 217 627 1568 1455 1842 Fragments arranged by size: 1842 1568 1455 627 325 217 BanII G_rGCy'C Cuts at: 1391 3153 3353 1391 Size: 1762 200 4072 Fragments arranged by size: 4072 1762 200 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2933 928 Size: 971 1034 4029 Fragments arranged by size: 4029 1034 971 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4225 4259 1903 Size: 34 2288 34 3678 Fragments arranged by size: 3678 2288 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2742 3311 3926 5136 2742 Size: 569 615 1210 3640 Fragments arranged by size: 3640 1210 615 569 BclI T'GATC_A Cuts at: 1548 1548 Size: 6034 BfrBI ATG'CAT Cuts at: 2825 2825 Size: 6034 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 3117 4645 136 Size: 107 122 71 631 605 1445 1528 1525 Fragments arranged by size: 1528 1525 1445 631 605 122 107 71 BglII A'GATC_T Cuts at: 6029 6029 Size: 6034 BlpI GC'TnA_GC Cuts at: 3451 3451 Size: 6034 Bme1580I G_kGCm'C Cuts at: 1790 2315 3091 4095 5251 1790 Size: 525 776 1004 1156 2573 Fragments arranged by size: 2573 1156 1004 776 525 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6034 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3765 4723 442 Size: 416 53 83 399 24 2348 958 1753 Fragments arranged by size: 2348 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6034 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2960 3198 4694 1239 Size: 1721 238 1496 2579 Fragments arranged by size: 2579 1721 1496 238 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2220 2584 2906 4159 5024 5322 2220 Size: 364 322 1253 865 298 2932 Fragments arranged by size: 2932 1253 865 364 322 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2676 4697 882 Size: 617 1177 2021 2219 Fragments arranged by size: 2219 2021 1177 617 BsaAI yAC'GTr Cuts at: 493 1204 3825 493 Size: 711 2621 2702 Fragments arranged by size: 2702 2621 711 BsaBI GATnn'nnATC Cuts at: 2634 6028 2634 Size: 3394 2640 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3841 4223 4858 275 Size: 53 83 186 936 2308 382 635 1451 Fragments arranged by size: 2308 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4467 4865 5139 5286 5697 4467 Size: 398 274 147 411 4804 Fragments arranged by size: 4804 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5809 Fragments arranged by size: 5809 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2056 2342 1186 Size: 870 286 4878 Fragments arranged by size: 4878 870 286 BseYI C'CCAG_C Cuts at: 2222 5237 5733 2222 Size: 3015 496 2523 Fragments arranged by size: 3015 2523 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 5461 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3484 4245 5273 1540 Size: 275 186 1483 761 1028 2301 Fragments arranged by size: 2301 1483 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2315 3091 3153 3259 4095 4180 5251 2315 Size: 776 62 106 836 85 1071 3098 Fragments arranged by size: 3098 1071 836 776 106 85 62 BsmI GAATG_Cn' Cuts at: 3545 3638 3545 Size: 93 5941 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2315 3091 3153 3259 3353 4095 4180 Size: 399 525 776 62 106 94 742 85 Cuts at: 4180 5251 1391 Size: 1071 2174 Fragments arranged by size: 2174 1071 776 742 525 399 106 94 85 62 BspEI T'CCGG_A Cuts at: 5697 5697 Size: 6034 BspHI T'CATG_A Cuts at: 3923 5653 3923 Size: 1730 4304 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3389 844 Size: 2545 3489 BsrBI CCG'CTC Cuts at: 1394 1823 3921 1394 Size: 429 2098 3507 Fragments arranged by size: 3507 2098 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2035 4529 4703 65 Size: 1183 787 2494 174 1396 Fragments arranged by size: 2494 1396 1183 787 174 BsrFI r'CCGG_y Cuts at: 1700 1810 3018 4678 4865 1700 Size: 110 1208 1660 187 2869 Fragments arranged by size: 2869 1660 1208 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6034 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6034 BssSI C'ACGA_G Cuts at: 4094 5106 4094 Size: 1012 5022 BstAPI GCAn_nnn'nTGC Cuts at: 794 2420 794 Size: 1626 4408 BstBI TT'CG_AA Cuts at: 3068 4838 3068 Size: 1770 4264 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6034 BstXI CCAn_nnnn'nTGG Cuts at: 3061 3061 Size: 6034 BstYI r'GATC_y Cuts at: 1972 3364 3731 4116 4133 5585 5671 5683 6029 Size: 1392 367 385 17 1452 86 12 346 Cuts at: 6029 1972 Size: 1977 Fragments arranged by size: 1977 1452 1392 385 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 2686 513 Size: 552 939 682 3861 Fragments arranged by size: 3861 939 682 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5295 Fragments arranged by size: 5295 683 56 BtsI GCAGTG_nn' Cuts at: 2141 3014 3550 4344 4364 2141 Size: 873 536 794 20 3811 Fragments arranged by size: 3811 873 794 536 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6034 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 2766 2801 529 Size: 35 2202 35 3762 Fragments arranged by size: 3762 2202 35 35 DraI TTT'AAA Cuts at: 3338 3338 Size: 6034 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5041 809 Size: 507 3725 1802 Fragments arranged by size: 3725 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2499 3481 4370 Size: 54 1425 50 275 18 669 982 889 Cuts at: 4370 5999 8 Size: 1629 43 Fragments arranged by size: 1629 1425 982 889 669 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3481 1537 Size: 275 1669 4090 Fragments arranged by size: 4090 1669 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2934 3964 1353 Size: 1581 1030 3423 Fragments arranged by size: 3423 1581 1030 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 3005 4621 4995 5141 387 Size: 1064 138 1416 1616 374 146 1280 Fragments arranged by size: 1616 1416 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 3151 3151 Size: 6034 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2184 2633 2960 3198 4097 4694 5481 Size: 393 552 449 327 238 899 597 787 Cuts at: 5481 5749 1239 Size: 268 1524 Fragments arranged by size: 1524 899 787 597 552 449 393 327 268 238 EcoNI CCTnn'n_nnAGG Cuts at: 2055 2055 Size: 6034 EcoO109I rG'GnC_Cy Cuts at: 2749 2749 Size: 6034 EcoRI G'AATT_C Cuts at: 3070 3343 3721 3070 Size: 273 378 5383 Fragments arranged by size: 5383 378 273 EcoRV GAT'ATC Cuts at: 2632 2632 Size: 6034 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2963 2963 Size: 6034 FspI TGC'GCA Cuts at: 4540 4540 Size: 6034 HaeII r_GCGC'y Cuts at: 1678 2947 5181 1678 Size: 1269 2234 2531 Fragments arranged by size: 2531 2234 1269 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 3246 3278 4675 4707 4749 Size: 32 135 32 1674 32 1397 32 42 Cuts at: 4749 4781 1373 Size: 32 2626 Fragments arranged by size: 2626 1674 1397 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3378 3624 669 Size: 988 281 1440 246 3079 Fragments arranged by size: 3079 1440 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6034 HpaI GTT'AAC Cuts at: 3624 3624 Size: 6034 KpnI G_GTAC'C Cuts at: 3359 3359 Size: 6034 MfeI C'AATT_G Cuts at: 3633 3633 Size: 6034 MluI A'CGCG_T Cuts at: 5910 5910 Size: 6034 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 5148 5332 1305 Size: 3843 184 2007 Fragments arranged by size: 3843 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 4212 Fragments arranged by size: 4212 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2184 3059 3992 4351 4510 518 Size: 648 1018 875 933 359 159 2042 Fragments arranged by size: 2042 1018 933 875 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2226 3450 4127 5275 5520 1606 Size: 620 1224 677 1148 245 2120 Fragments arranged by size: 2120 1224 1148 677 620 245 NaeI GCC'GGC Cuts at: 3020 3020 Size: 6034 NcoI C'CATG_G Cuts at: 513 1065 2004 2686 513 Size: 552 939 682 3861 Fragments arranged by size: 3861 939 682 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 6034 NgoMIV G'CCGG_C Cuts at: 3018 3018 Size: 6034 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6034 NotI GC'GGCC_GC Cuts at: 3481 3481 Size: 6034 NsiI A_TGCA'T Cuts at: 2827 2827 Size: 6034 NspI r_CATG'y Cuts at: 1132 1229 2512 2609 3392 4937 1132 Size: 97 1283 97 783 1545 2229 Fragments arranged by size: 2229 1545 1283 783 97 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6034 PciI A'CATG_T Cuts at: 4933 4933 Size: 6034 PflMI CCAn_nnn'nTGG Cuts at: 1108 2827 1108 Size: 1719 4315 PfoI T'CCnGG_A Cuts at: 2745 2985 2745 Size: 240 5794 PmeI GTTT'AAAC Cuts at: 3338 3338 Size: 6034 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4103 4135 5616 5648 4103 Size: 32 1481 32 4489 Fragments arranged by size: 4489 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2749 2749 Size: 6034 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6034 PsiI TTA'TAA Cuts at: 2678 3604 2678 Size: 926 5108 PspXI vC'TCGA_Gb Cuts at: 2145 2517 2145 Size: 372 5662 PstI C_TGCA'G Cuts at: 830 3386 830 Size: 2556 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6034 PvuII CAG'CTG Cuts at: 2226 2226 Size: 6034 SacI G_AGCT'C Cuts at: 3153 3153 Size: 6034 SalI G'TCGA_C Cuts at: 1936 3376 1936 Size: 1440 4594 SapI GCTCTTCn'nnn_ Cuts at: 2934 2934 Size: 6034 SbfI CC_TGCA'GG Cuts at: 3386 3386 Size: 6034 ScaI AGT'ACT Cuts at: 4282 5994 4282 Size: 1712 4322 SfcI C'TryA_G Cuts at: 826 1045 2448 3382 4517 5198 5389 826 Size: 219 1403 934 1135 681 191 1471 Fragments arranged by size: 1471 1403 1135 934 681 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6034 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6034 SmaI CCC'GGG Cuts at: 1872 3361 1872 Size: 1489 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2145 2235 2517 2563 2921 3235 Size: 197 926 202 90 282 46 358 314 Cuts at: 3235 4138 5039 5301 820 Size: 903 901 262 1553 Fragments arranged by size: 1553 926 903 901 358 314 282 262 202 197 90 46 SnaBI TAC'GTA Cuts at: 493 3825 493 Size: 3332 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6034 SphI G_CATG'C Cuts at: 1132 3392 1132 Size: 2260 3774 SspI AAT'ATT Cuts at: 5 52 3958 5 Size: 47 3906 2081 Fragments arranged by size: 3906 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6034 StyI C'CwwG_G Cuts at: 513 1065 2004 2322 2686 2736 3473 513 Size: 552 939 318 364 50 737 3074 Fragments arranged by size: 3074 939 737 552 364 318 50 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 4061 4078 4231 4416 4861 325 Size: 1255 2481 17 153 185 445 1498 Fragments arranged by size: 2481 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2157 4280 5992 Size: 274 80 33 51 1443 180 2123 1712 Cuts at: 5992 96 Size: 138 Fragments arranged by size: 2123 1712 1443 274 180 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2419 2451 5994 6026 2419 Size: 32 3543 32 2427 Fragments arranged by size: 3543 2427 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6034 XbaI T'CTAG_A Cuts at: 3370 3370 Size: 6034 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2011 2011 Size: 6034 XhoI C'TCGA_G Cuts at: 1943 2145 2517 1943 Size: 202 372 5460 Fragments arranged by size: 5460 372 202 XmaI C'CCGG_G Cuts at: 1870 3359 1870 Size: 1489 4545 XmnI GAAnn'nnTTC Cuts at: 2354 4163 2354 Size: 1809 4225 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3842 276 Size: 53 83 186 936 2308 2468 Fragments arranged by size: 2468 2308 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BaeI BamHI BanI BanII BbsI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI EcoRV FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NsiI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspXI PstI PvuI PvuII SacI SalI SapI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BbeI BbvCI BplI Bpu10I BsiWI BsmBI BstZ17I Bsu36I DraIII FseI FspAI KasI NarI NruI PacI PmlI PspOMI PsrI RsrII SacII SanDI SexAI SfiI SfoI SrfI SwaI