Restriction map for
pFN21AB7013
(Circular) (Six-base) MAPSORT of: pFN21AB7013.seq Check: 3180 from: 1 to: 7963
pFN21AB7013 7963 bp
With 182 enzymes: SgfI *
January 16, 2009 15:25 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2321 3710 5104 2321
Size: 1389 1394 5180
Fragments arranged by size:
5180 1394 1389
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 5773 278
Size: 53 83 186 936 4237 2468
Fragments arranged by size:
4237 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2080 2937 5306 1937
Size: 143 857 2369 4594
Fragments arranged by size:
4594 2369 857 143
Acc65I G'GTAC_C
Cuts at: 4055 5284 4055
Size: 1229 6734
AclI AA'CG_TT
Cuts at: 1563 6090 6463 1563
Size: 4527 373 3063
Fragments arranged by size:
4527 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3212 3394 6026 7410 7678 1632
Size: 1580 182 2632 1384 268 1917
Fragments arranged by size:
2632 1917 1580 1384 268 182
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 7766
AflIII A'CryG_T
Cuts at: 1203 2083 3753 5219 6862 7839 1203
Size: 880 1670 1466 1643 977 1327
Fragments arranged by size:
1670 1643 1466 1327 977 880
AgeI A'CCGG_T
Cuts at: 6794 6794
Size: 7963
AhdI GACnn_n'nnGTC
Cuts at: 6692 6692
Size: 7963
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 7963
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 7963
AlwNI CAG_nnn'CTG
Cuts at: 2669 3371 4439 5003 5706 7278 2669
Size: 702 1068 564 703 1572 3354
Fragments arranged by size:
3354 1572 1068 703 702 564
ApaI G_GGCC'C
Cuts at: 4013 4540 4013
Size: 527 7436
ApaLI G'TGCA_C
Cuts at: 4590 6020 7176 4590
Size: 1430 1156 5377
Fragments arranged by size:
5377 1430 1156
ApoI r'AATT_y
Cuts at: 1493 2157 4716 4887 5272 5498 5650 7759 1493
Size: 664 2559 171 385 226 152 2109 1697
Fragments arranged by size:
2559 2109 1697 664 385 226 171 152
AseI AT'TA_AT
Cuts at: 160 1032 6517 160
Size: 872 5485 1606
Fragments arranged by size:
5485 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7963
AvaI C'yCGr_G
Cuts at: 1870 1943 3883 5288 1870
Size: 73 1940 1405 4545
Fragments arranged by size:
4545 1940 1405 73
AvrII C'CTAG_G
Cuts at: 3877 3877
Size: 7963
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 3638 3671 3638
Size: 33 7930
BamHI G'GATC_C
Cuts at: 5293 5660 5293
Size: 367 7596
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2333 3344 3812 3902 4055
Size: 325 217 627 546 1011 468 90 153
Cuts at: 4055 4682 5284 6739 618
Size: 627 602 1455 1842
Fragments arranged by size:
1842 1455 1011 627 627 602 546 468
325 217 153 90
BanII G_rGCy'C
Cuts at: 1391 2395 2823 4013 4540 5282 1391
Size: 1004 428 1190 527 742 4072
Fragments arranged by size:
4072 1190 1004 742 527 428
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 3368 3422 928
Size: 971 1469 54 5469
Fragments arranged by size:
5469 1469 971 54
BbvCI CC'TCA_GC
Cuts at: 3048 4938 3048
Size: 1890 6073
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2727 2761 6154 6188 1903
Size: 34 790 34 3393 34 3678
Fragments arranged by size:
3678 3393 790 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3453 3666 5855 7065 3453
Size: 213 2189 1210 4351
Fragments arranged by size:
4351 2189 1210 213
BclI T'GATC_A
Cuts at: 1548 2314 1548
Size: 766 7197
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 3152 4333 6574
Size: 107 122 71 631 605 1480 1181 2241
Cuts at: 6574 136
Size: 1525
Fragments arranged by size:
2241 1525 1480 1181 631 605 122 107
71
BglII A'GATC_T
Cuts at: 7958 7958
Size: 7963
BlpI GC'TnA_GC
Cuts at: 2579 2817 3372 5380 2579
Size: 238 555 2008 5162
Fragments arranged by size:
5162 2008 555 238
Bme1580I G_kGCm'C
Cuts at: 1790 2338 3349 4013 4540 4594 6024 7180 1790
Size: 548 1011 664 527 54 1430 1156 2573
Fragments arranged by size:
2573 1430 1156 1011 664 548 527 54
BmgBI CAC'GTC
Cuts at: 1318 3938 1318
Size: 2620 5343
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3344 3473 3962
Size: 416 53 83 399 24 1927 129 489
Cuts at: 3962 4889 5694 6652 442
Size: 927 805 958 1753
Fragments arranged by size:
1927 1753 958 927 805 489 416 399
129 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 7963
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2667 2964 3993 4056 5216 6623 1239
Size: 1428 297 1029 63 1160 1407 2579
Fragments arranged by size:
2579 1428 1407 1160 1029 297 63
Bpu10I CC'TnA_GC
Cuts at: 2562 3048 4938 2562
Size: 486 1890 5587
Fragments arranged by size:
5587 1890 486
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3324 5037 5114 6088 6953 7251 3324
Size: 1713 77 974 865 298 4036
Fragments arranged by size:
4036 1713 974 865 298 77
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 3455 4275 4304 4811 6626 882
Size: 617 1956 820 29 507 1815 2219
Fragments arranged by size:
2219 1956 1815 820 617 507 29
BsaAI yAC'GTr
Cuts at: 493 1204 2084 5754 493
Size: 711 880 3670 2702
Fragments arranged by size:
3670 2702 880 711
BsaBI GATnn'nnATC
Cuts at: 7957 7957
Size: 7963
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 4254 5770 6152 6787
Size: 53 83 186 936 2721 1516 382 635
Cuts at: 6787 275
Size: 1451
Fragments arranged by size:
2721 1516 1451 936 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 6396 6794 7068 7215 7626 6396
Size: 398 274 147 411 6733
Fragments arranged by size:
6733 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 7738
Fragments arranged by size:
7738 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 7963
BseYI C'CCAG_C
Cuts at: 2473 2717 3134 3313 4533 7166 7662 2473
Size: 244 417 179 1220 2633 496 2774
Fragments arranged by size:
2774 2633 1220 496 417 244 179
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 4566 4833 1163
Size: 573 2830 267 4293
Fragments arranged by size:
4293 2830 573 267
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 5413 6174 7202 1540
Size: 275 186 3412 761 1028 2301
Fragments arranged by size:
3412 2301 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 4594 6024 6109 7180 4594
Size: 1430 85 1071 5377
Fragments arranged by size:
5377 1430 1071 85
BsmI GAATG_Cn'
Cuts at: 3695 3750 4914 5474 5567 3695
Size: 55 1164 560 93 6091
Fragments arranged by size:
6091 1164 560 93 55
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2338 2395 2823 3349 4013 4540 4594
Size: 399 548 57 428 526 664 527 54
Cuts at: 4594 5282 6024 6109 7180 1391
Size: 688 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 688 664 548 527 526
428 399 85 57 54
BspEI T'CCGG_A
Cuts at: 7626 7626
Size: 7963
BspHI T'CATG_A
Cuts at: 2422 5852 7582 2422
Size: 3430 1730 2803
Fragments arranged by size:
3430 2803 1730
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2321 3138 3437 3710 3807 5104 5318 844
Size: 1477 817 299 273 97 1297 214 3489
Fragments arranged by size:
3489 1477 1297 817 299 273 214 97
BsrBI CCG'CTC
Cuts at: 1394 1823 5850 1394
Size: 429 4027 3507
Fragments arranged by size:
4027 3507 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2538 2595 3474 3714 3952 4275 4647
Size: 1183 1290 57 879 240 238 323 372
Cuts at: 4647 4833 6458 6632 65
Size: 186 1625 174 1396
Fragments arranged by size:
1625 1396 1290 1183 879 372 323 240
238 186 174 57
BsrFI r'CCGG_y
Cuts at: 1700 1810 3809 6607 6794 1700
Size: 110 1999 2798 187 2869
Fragments arranged by size:
2869 2798 1999 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 7963
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 7963
BssSI C'ACGA_G
Cuts at: 2041 6023 7035 2041
Size: 3982 1012 2969
Fragments arranged by size:
3982 2969 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 7963
BstBI TT'CG_AA
Cuts at: 6767 6767
Size: 7963
BstEII G'GTnAC_C
Cuts at: 1184 3508 1184
Size: 2324 5639
BstXI CCAn_nnnn'nTGG
Cuts at: 3142 4225 3142
Size: 1083 6880
BstYI r'GATC_y
Cuts at: 1972 5059 5293 5660 6045 6062 7514 7600 7612
Size: 3087 234 367 385 17 1452 86 12
Cuts at: 7612 7958 1972
Size: 346 1977
Fragments arranged by size:
3087 1977 1452 385 367 346 234 86
17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2586 4710 513
Size: 552 939 582 2124 3766
Fragments arranged by size:
3766 2124 939 582 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 4055 487
Size: 683 56 2829 4395
Fragments arranged by size:
4395 2829 683 56
BtsI GCAGTG_nn'
Cuts at: 5163 5479 6273 6293 5163
Size: 316 794 20 6833
Fragments arranged by size:
6833 794 316 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 7963
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 2111 2146 529
Size: 35 1547 35 6346
Fragments arranged by size:
6346 1547 35 35
DraI TTT'AAA
Cuts at: 2439 5267 2439
Size: 2828 5135
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4499 6970 809
Size: 507 3183 2471 1802
Fragments arranged by size:
3183 2471 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2589 3144 5190
Size: 54 1425 50 275 18 759 555 2046
Cuts at: 5190 5410 6299 7928 8
Size: 220 889 1629 43
Fragments arranged by size:
2046 1629 1425 889 759 555 275 220
54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 5410 1537
Size: 275 3598 4090
Fragments arranged by size:
4090 3598 275
EarI CTCTTCn'nnn_
Cuts at: 1353 5123 5893 1353
Size: 3770 770 3423
Fragments arranged by size:
3770 3423 770
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 6550 6924 7070 387
Size: 1064 138 4961 374 146 1280
Fragments arranged by size:
4961 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2667 2964 3212 3394 3993 4056 5216
Size: 393 1035 297 248 182 599 63 1160
Cuts at: 5216 6026 6623 7410 7678 1239
Size: 810 597 787 268 1524
Fragments arranged by size:
1524 1160 1035 810 787 599 597 393
297 268 248 182 63
EcoO109I rG'GnC_Cy
Cuts at: 2067 3676 4537 2067
Size: 1609 861 5493
Fragments arranged by size:
5493 1609 861
EcoRI G'AATT_C
Cuts at: 2157 4887 5272 5650 2157
Size: 2730 385 378 4470
Fragments arranged by size:
4470 2730 385 378
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2450 2450
Size: 7963
FspI TGC'GCA
Cuts at: 6469 6469
Size: 7963
HaeII r_GCGC'y
Cuts at: 1678 2144 7110 1678
Size: 466 4966 2531
Fragments arranged by size:
4966 2531 466
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 3553 3585 5051 5083 6604
Size: 32 135 32 1981 32 1466 32 1521
Cuts at: 6604 6636 6678 6710 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1981 1521 1466 135 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3426 3712 5307 5553 669
Size: 988 281 1488 286 1595 246 3079
Fragments arranged by size:
3079 1595 1488 988 286 281 246
HindIII A'AGCT_T
Cuts at: 748 2295 4402 748
Size: 1547 2107 4309
Fragments arranged by size:
4309 2107 1547
HpaI GTT'AAC
Cuts at: 5553 5553
Size: 7963
KpnI G_GTAC'C
Cuts at: 4059 5288 4059
Size: 1229 6734
MfeI C'AATT_G
Cuts at: 2556 2757 4071 4755 5562 2556
Size: 201 1314 684 807 4957
Fragments arranged by size:
4957 1314 807 684 201
MluI A'CGCG_T
Cuts at: 7839 7839
Size: 7963
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2161 2432 3195 4947 7077 7261 1305
Size: 856 271 763 1752 2130 184 2007
Fragments arranged by size:
2130 2007 1752 856 763 271 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2591 3146 10
Size: 54 1425 343 759 555 4827
Fragments arranged by size:
4827 1425 759 555 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 4460 4634 5192 5921 6280 6439 518
Size: 648 3294 174 558 729 359 159 2042
Fragments arranged by size:
3294 2042 729 648 558 359 174 159
MspA1I CmG'CkG
Cuts at: 1606 2666 4145 4475 4697 5379 6056 7204 7449
Size: 1060 1479 330 222 682 677 1148 245
Cuts at: 7449 1606
Size: 2120
Fragments arranged by size:
2120 1479 1148 1060 682 677 330 245
222
NaeI GCC'GGC
Cuts at: 3811 3811
Size: 7963
NcoI C'CATG_G
Cuts at: 513 1065 2004 2586 4710 513
Size: 552 939 582 2124 3766
Fragments arranged by size:
3766 2124 939 582 552
NdeI CA'TA_TG
Cuts at: 387 4954 387
Size: 4567 3396
NgoMIV G'CCGG_C
Cuts at: 3809 3809
Size: 7963
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 7963
NotI GC'GGCC_GC
Cuts at: 5410 5410
Size: 7963
NspI r_CATG'y
Cuts at: 1132 1229 3757 4498 5086 5223 5321 6866 1132
Size: 97 2528 741 588 137 98 1545 2229
Fragments arranged by size:
2528 2229 1545 741 588 137 98 97
PasI CC'CwG_GG
Cuts at: 1348 3925 1348
Size: 2577 5386
PciI A'CATG_T
Cuts at: 3753 5219 6862 3753
Size: 1466 1643 4854
Fragments arranged by size:
4854 1643 1466
PflMI CCAn_nnn'nTGG
Cuts at: 1108 4802 1108
Size: 3694 4269
PmeI GTTT'AAAC
Cuts at: 5267 5267
Size: 7963
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4511 4543 6032 6064 7545 7577 4511
Size: 32 1489 32 1481 32 4897
Fragments arranged by size:
4897 1489 1481 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 2067 2067
Size: 7963
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 7963
PsiI TTA'TAA
Cuts at: 2607 3855 5533 2607
Size: 1248 1678 5037
Fragments arranged by size:
5037 1678 1248
PspOMI G'GGCC_C
Cuts at: 4009 4536 4009
Size: 527 7436
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 2098 2130 2098
Size: 32 7931
PstI C_TGCA'G
Cuts at: 830 2734 2788 3622 5158 5315 830
Size: 1904 54 834 1536 157 3478
Fragments arranged by size:
3478 1904 1536 834 157 54
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 7963
PvuII CAG'CTG
Cuts at: 2666 4145 4475 4697 2666
Size: 1479 330 222 5932
Fragments arranged by size:
5932 1479 330 222
SalI G'TCGA_C
Cuts at: 1936 5305 1936
Size: 3369 4594
SbfI CC_TGCA'GG
Cuts at: 5315 5315
Size: 7963
ScaI AGT'ACT
Cuts at: 6211 7923 6211
Size: 1712 6251
SexAI A'CCwGG_T
Cuts at: 2538 2538
Size: 7963
SfcI C'TryA_G
Cuts at: 826 1045 2320 2520 2730 2784 3282 3618 3844
Size: 219 1275 200 210 54 498 336 226
Cuts at: 3844 5154 5311 6446 7127 7318 826
Size: 1310 157 1135 681 191 1471
Fragments arranged by size:
1471 1310 1275 1135 681 498 336 226
219 210 200 191 157 54
SfiI GGCCn_nnn'nGGCC
Cuts at: 3152 3152
Size: 7963
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7963
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 7963
SmaI CCC'GGG
Cuts at: 1872 5290 1872
Size: 3418 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3339 5052 5093 6067 6968 7230
Size: 197 926 1396 1713 41 974 901 262
Cuts at: 7230 820
Size: 1553
Fragments arranged by size:
1713 1553 1396 974 926 901 262 197
41
SnaBI TAC'GTA
Cuts at: 493 5754 493
Size: 5261 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 7963
SphI G_CATG'C
Cuts at: 1132 5086 5321 1132
Size: 3954 235 3774
Fragments arranged by size:
3954 3774 235
SspI AAT'ATT
Cuts at: 5 52 3334 5887 5
Size: 47 3282 2553 2081
Fragments arranged by size:
3282 2553 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 7963
StyI C'CwwG_G
Cuts at: 513 1065 2004 2344 2586 2823 3877 3906 4710
Size: 552 939 340 242 237 1054 29 804
Cuts at: 4710 5402 513
Size: 692 3074
Fragments arranged by size:
3074 1054 939 804 692 552 340 242
237 29
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2741 3938 4040 4448 4577 4908 5129
Size: 1255 1161 1197 102 408 129 331 221
Cuts at: 5129 5990 6007 6160 6345 6790 325
Size: 861 17 153 185 445 1498
Fragments arranged by size:
1498 1255 1197 1161 861 445 408 331
221 185 153 129 102 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4147 4167 6209
Size: 274 80 33 51 1443 2170 20 2042
Cuts at: 6209 7921 96
Size: 1712 138
Fragments arranged by size:
2170 2042 1712 1443 274 138 80 51
33 20
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3270 3302 7923 7955 3270
Size: 32 4621 32 3278
Fragments arranged by size:
4621 3278 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 3421 1316
Size: 2105 5858
XbaI T'CTAG_A
Cuts at: 5299 5299
Size: 7963
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 3520 4840 3520
Size: 1320 6643
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 7963
XmaI C'CCGG_G
Cuts at: 1870 5288 1870
Size: 3418 4545
XmnI GAAnn'nnTTC
Cuts at: 6092 6092
Size: 7963
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 5771 276
Size: 53 83 186 936 4237 2468
Fragments arranged by size:
4237 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI AvrII BaeI BamHI BanI BanII
BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI
Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII
BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI
DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI
FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI
MfeI MluI MmeI MscI MslI MspA1I NaeI NcoI
NdeI NgoMIV NheI NotI NspI PasI PciI PflMI
PmeI PpiI PpuMI PshAI PsiI PspOMI PsrI PstI
PvuI PvuII SalI SbfI ScaI SexAI SfcI SfiI
SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI
StuI StyI TaqII TatI TstI Tth111I XbaI XcmI
XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AloI AscI BbeI BfrBI BplI BsiWI BsmBI
BstZ17I Bsu36I DraIII EcoICRI EcoNI EcoRV FseI FspAI
KasI NarI NruI NsiI PacI PfoI PmlI PspXI
RsrII SacI SacII SanDI SapI SfoI SrfI SwaI