(Circular) (Six-base) MAPSORT of: pFN21AB7009.seq Check: 4950 from: 1 to: 7921 pFN21AB7009 7921 bp With 182 enzymes: SgfI * January 16, 2009 15:25 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 3497 3497 Size: 7921 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 5731 278 Size: 53 83 186 936 4195 2468 Fragments arranged by size: 4195 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2846 3954 5264 1937 Size: 909 1108 1310 4594 Fragments arranged by size: 4594 1310 1108 909 Acc65I G'GTAC_C Cuts at: 2795 5185 5242 2795 Size: 2390 57 5474 Fragments arranged by size: 5474 2390 57 AclI AA'CG_TT Cuts at: 1563 6048 6421 1563 Size: 4485 373 3063 Fragments arranged by size: 4485 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3182 4580 4814 5984 7368 7636 1632 Size: 1550 1398 234 1170 1384 268 1917 Fragments arranged by size: 1917 1550 1398 1384 1170 268 234 AfeI AGC'GCT Cuts at: 5099 5099 Size: 7921 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 7724 AflIII A'CryG_T Cuts at: 1203 6820 7797 1203 Size: 5617 977 1327 Fragments arranged by size: 5617 1327 977 AgeI A'CCGG_T Cuts at: 6752 6752 Size: 7921 AhdI GACnn_n'nnGTC Cuts at: 6650 6650 Size: 7921 AleI CACnn'nnGTG Cuts at: 1166 3557 3810 1166 Size: 2391 253 5277 Fragments arranged by size: 5277 2391 253 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2457 2940 3203 3225 3575 1184 Size: 1273 483 263 22 350 5530 Fragments arranged by size: 5530 1273 483 350 263 22 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3001 3033 4373 4405 3001 Size: 32 1340 32 6517 Fragments arranged by size: 6517 1340 32 32 AlwNI CAG_nnn'CTG Cuts at: 2524 2710 2776 3104 3420 3931 4144 4704 4837 Size: 186 66 328 316 511 213 560 133 Cuts at: 4837 4898 5173 5664 7236 2524 Size: 61 275 491 1572 3209 Fragments arranged by size: 3209 1572 560 511 491 328 316 275 213 186 133 66 61 ApaI G_GGCC'C Cuts at: 3387 3829 4120 4888 3387 Size: 442 291 768 6420 Fragments arranged by size: 6420 768 442 291 ApaLI G'TGCA_C Cuts at: 4159 5978 7134 4159 Size: 1819 1156 4946 Fragments arranged by size: 4946 1819 1156 ApoI r'AATT_y Cuts at: 1493 2576 5230 5456 5608 7717 1493 Size: 1083 2654 226 152 2109 1697 Fragments arranged by size: 2654 2109 1697 1083 226 152 AseI AT'TA_AT Cuts at: 160 1032 6475 160 Size: 872 5443 1606 Fragments arranged by size: 5443 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 7921 AvaI C'yCGr_G Cuts at: 1870 1943 2982 3168 3271 3547 4647 4785 4887 Size: 73 1039 186 103 276 1100 138 102 Cuts at: 4887 5246 1870 Size: 359 4545 Fragments arranged by size: 4545 1100 1039 359 276 186 138 103 102 73 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 2275 2308 2275 Size: 33 7888 BamHI G'GATC_C Cuts at: 5137 5251 5618 5137 Size: 114 367 7440 Fragments arranged by size: 7440 367 114 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2795 3509 3559 3644 5185 Size: 325 217 627 1008 714 50 85 1541 Cuts at: 5185 5242 6697 618 Size: 57 1455 1842 Fragments arranged by size: 1842 1541 1455 1008 714 627 325 217 85 57 50 BanII G_rGCy'C Cuts at: 1391 2199 2484 2810 3278 3387 3753 3829 3861 Size: 808 285 326 468 109 366 76 32 Cuts at: 3861 3897 4120 4888 4947 5240 1391 Size: 36 223 768 59 293 4072 Fragments arranged by size: 4072 808 768 468 366 326 293 285 223 109 76 59 36 32 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2547 928 Size: 971 648 6302 Fragments arranged by size: 6302 971 648 BbvCI CC'TCA_GC Cuts at: 2271 2824 2271 Size: 553 7368 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 1987 2021 2836 2870 4499 4533 4638 Size: 34 50 34 815 34 1629 34 105 Cuts at: 4638 4672 6112 6146 1903 Size: 34 1440 34 3678 Fragments arranged by size: 3678 1629 1440 815 105 50 34 34 34 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 5213 5813 7023 5213 Size: 600 1210 6111 Fragments arranged by size: 6111 1210 600 BclI T'GATC_A Cuts at: 1548 1548 Size: 7921 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 6532 136 Size: 107 122 71 631 605 4860 1525 Fragments arranged by size: 4860 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 3525 7916 3525 Size: 4391 3530 BlpI GC'TnA_GC Cuts at: 2483 3612 4280 5338 2483 Size: 1129 668 1058 5066 Fragments arranged by size: 5066 1129 1058 668 Bme1580I G_kGCm'C Cuts at: 1790 3387 3406 3554 3829 4120 4163 4238 4405 Size: 1597 19 148 275 291 43 75 167 Cuts at: 4405 4888 5982 7138 1790 Size: 483 1094 1156 2573 Fragments arranged by size: 2573 1597 1156 1094 483 291 275 167 148 75 43 19 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 7921 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2805 2836 3408 Size: 416 53 83 399 24 1388 31 572 Cuts at: 3408 4399 4453 4672 5652 6610 442 Size: 991 54 219 980 958 1753 Fragments arranged by size: 1753 1388 991 980 958 572 416 399 219 83 54 53 31 24 BmtI G_CTAG'C Cuts at: 1056 2138 1056 Size: 1082 6839 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3547 4811 3547 Size: 1264 6657 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2117 2261 2546 3051 3455 3620 3737 3773 Size: 878 144 285 505 404 165 117 36 Cuts at: 3773 4463 4860 4920 4959 5102 5151 6581 1239 Size: 690 397 60 39 143 49 1430 2579 Fragments arranged by size: 2579 1430 878 690 505 404 397 285 165 144 143 117 60 49 39 36 Bpu10I CC'TnA_GC Cuts at: 2271 2804 2824 3891 4946 2271 Size: 533 20 1067 1055 5246 Fragments arranged by size: 5246 1067 1055 533 20 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 6046 6911 7209 6046 Size: 865 298 6758 Fragments arranged by size: 6758 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 3789 6584 882 Size: 617 2290 2795 2219 Fragments arranged by size: 2795 2290 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 5110 5712 493 Size: 711 3906 602 2702 Fragments arranged by size: 3906 2702 711 602 BsaBI GATnn'nnATC Cuts at: 7915 7915 Size: 7921 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2160 3394 5728 6110 Size: 53 83 186 936 627 1234 2334 382 Cuts at: 6110 6745 275 Size: 635 1451 Fragments arranged by size: 2334 1451 1234 936 635 627 382 186 83 53 BsaWI w'CCGG_w Cuts at: 3303 4131 6354 6752 7026 7173 7584 3303 Size: 828 2223 398 274 147 411 3640 Fragments arranged by size: 3640 2223 828 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2519 2549 3740 3770 3801 Size: 30 165 30 1116 30 1191 30 31 Cuts at: 3801 3831 4858 4888 1178 Size: 30 1027 30 4211 Fragments arranged by size: 4211 1191 1116 1027 165 31 30 30 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2036 2063 2083 2396 2435 3536 3728 3791 Size: 850 27 20 313 39 1101 192 63 Cuts at: 3791 4517 4665 1186 Size: 726 148 4442 Fragments arranged by size: 4442 1101 850 726 313 192 148 63 39 27 20 BseYI C'CCAG_C Cuts at: 3141 3149 3282 3640 3965 3969 4113 4880 5053 Size: 8 133 358 325 4 144 767 173 Cuts at: 5053 5118 7124 7620 3141 Size: 65 2006 496 3442 Fragments arranged by size: 3442 2006 767 496 358 325 173 144 133 65 8 4 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2360 2669 3764 4754 5090 1163 Size: 573 624 309 1095 990 336 3994 Fragments arranged by size: 3994 1095 990 624 573 336 309 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2111 2254 5371 6132 7160 1540 Size: 275 186 110 143 3117 761 1028 2301 Fragments arranged by size: 3117 2301 1028 761 275 186 143 110 BsiHKAI G_wGCw'C Cuts at: 2436 2484 2758 2905 2961 3753 4163 4412 5982 Size: 48 274 147 56 792 410 249 1570 Cuts at: 5982 6067 7138 2436 Size: 85 1071 3219 Fragments arranged by size: 3219 1570 1071 792 410 274 249 147 85 56 48 BsiWI C'GTAC_G Cuts at: 4635 5110 4635 Size: 475 7446 BsmI GAATG_Cn' Cuts at: 5432 5525 5432 Size: 93 7828 BsmBI CGTCTCn'nnnn_ Cuts at: 5078 5078 Size: 7921 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2199 2436 2484 2758 2810 2905 2961 Size: 399 409 237 48 274 52 95 56 Cuts at: 2961 3278 3387 3406 3554 3753 3829 3861 3897 Size: 317 109 19 148 199 76 32 36 Cuts at: 3897 4120 4163 4238 4405 4412 4888 4947 5240 Size: 223 43 75 167 7 476 59 293 Cuts at: 5240 5982 6067 7138 1391 Size: 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 476 409 399 317 293 274 237 223 199 167 148 109 95 85 76 75 59 56 52 48 43 36 32 19 7 BspEI T'CCGG_A Cuts at: 7584 7584 Size: 7921 BspHI T'CATG_A Cuts at: 5810 7540 5810 Size: 1730 6191 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2157 2827 3497 4812 5112 5276 844 Size: 1313 670 670 1315 300 164 3489 Fragments arranged by size: 3489 1315 1313 670 670 300 164 BsrBI CCG'CTC Cuts at: 1394 1823 2026 5808 1394 Size: 429 203 3782 3507 Fragments arranged by size: 3782 3507 429 203 BsrDI GCAATG_nn' Cuts at: 65 1248 4593 6416 6590 65 Size: 1183 3345 1823 174 1396 Fragments arranged by size: 3345 1823 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4988 6565 6752 1700 Size: 110 3178 1577 187 2869 Fragments arranged by size: 3178 2869 1577 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 7921 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 7921 BssSI C'ACGA_G Cuts at: 5981 6993 5981 Size: 1012 6909 BstAPI GCAn_nnn'nTGC Cuts at: 794 3332 3574 4614 4704 794 Size: 2538 242 1040 90 4011 Fragments arranged by size: 4011 2538 1040 242 90 BstBI TT'CG_AA Cuts at: 6725 6725 Size: 7921 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 7921 BstXI CCAn_nnnn'nTGG Cuts at: 3029 3157 3358 3029 Size: 128 201 7592 Fragments arranged by size: 7592 201 128 BstYI r'GATC_y Cuts at: 1972 3427 3525 5137 5251 5618 6003 6020 7472 Size: 1455 98 1612 114 367 385 17 1452 Cuts at: 7472 7558 7570 7916 1972 Size: 86 12 346 1977 Fragments arranged by size: 1977 1612 1455 1452 385 367 346 114 98 86 17 12 Bsu36I CC'TnA_GG Cuts at: 2066 3774 2066 Size: 1708 6213 BtgI C'CryG_G Cuts at: 513 1065 2223 2737 2834 513 Size: 552 1158 514 97 5600 Fragments arranged by size: 5600 1158 552 514 97 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 4183 487 Size: 683 56 2957 4225 Fragments arranged by size: 4225 2957 683 56 BtsI GCAGTG_nn' Cuts at: 2681 2686 2961 3575 3995 5437 6231 6251 2681 Size: 5 275 614 420 1442 794 20 4351 Fragments arranged by size: 4351 1442 794 614 420 275 20 5 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 7921 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 7886 DraI TTT'AAA Cuts at: 5225 5225 Size: 7921 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4853 6928 809 Size: 507 3537 2075 1802 Fragments arranged by size: 3537 2075 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2108 2251 4246 Size: 54 1425 50 275 18 278 143 1995 Cuts at: 4246 4774 5088 5368 6257 7886 8 Size: 528 314 280 889 1629 43 Fragments arranged by size: 1995 1629 1425 889 528 314 280 278 275 143 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2108 2251 5368 1537 Size: 275 296 143 3117 4090 Fragments arranged by size: 4090 3117 296 275 143 EarI CTCTTCn'nnn_ Cuts at: 1353 2013 4802 5070 5175 5851 1353 Size: 660 2789 268 105 676 3423 Fragments arranged by size: 3423 2789 676 660 268 105 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2756 3854 4172 5230 6508 6882 Size: 1064 138 1167 1098 318 1058 1278 374 Cuts at: 6882 7028 387 Size: 146 1280 Fragments arranged by size: 1280 1278 1167 1098 1064 1058 374 318 146 138 EcoICRI GAG'CTC Cuts at: 2482 3751 2482 Size: 1269 6652 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2117 2261 2546 3051 3182 3455 3620 Size: 393 485 144 285 505 131 273 165 Cuts at: 3620 3737 3773 4463 4580 4814 4860 4920 4959 Size: 117 36 690 117 234 46 60 39 Cuts at: 4959 5102 5151 5984 6581 7368 7636 1239 Size: 143 49 833 597 787 268 1524 Fragments arranged by size: 1524 833 787 690 597 505 485 393 285 273 268 234 165 144 143 131 117 117 60 49 46 39 36 EcoNI CCTnn'n_nnAGG Cuts at: 3200 4997 3200 Size: 1797 6124 EcoO109I rG'GnC_Cy Cuts at: 2707 2785 3017 3368 3384 3631 3826 4117 4141 Size: 78 232 351 16 247 195 291 24 Cuts at: 4141 4381 4579 4884 2707 Size: 240 198 305 5744 Fragments arranged by size: 5744 351 305 291 247 240 232 198 195 78 24 16 EcoRI G'AATT_C Cuts at: 5230 5608 5230 Size: 378 7543 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 5036 5036 Size: 7921 FspI TGC'GCA Cuts at: 6427 6427 Size: 7921 HaeII r_GCGC'y Cuts at: 1678 2064 3445 3847 3874 4495 5101 7068 1678 Size: 386 1381 402 27 621 606 1967 2531 Fragments arranged by size: 2531 1967 1381 621 606 402 386 27 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 3740 3767 3772 3799 5187 Size: 32 135 32 2168 27 5 27 1388 Cuts at: 5187 5219 6562 6594 6636 6668 1373 Size: 32 1343 32 42 32 2626 Fragments arranged by size: 2626 2168 1388 1343 135 42 32 32 32 32 32 27 27 5 HincII GTy'rAC Cuts at: 669 1657 1938 2847 5265 5511 669 Size: 988 281 909 2418 246 3079 Fragments arranged by size: 3079 2418 988 909 281 246 HindIII A'AGCT_T Cuts at: 748 4956 748 Size: 4208 3713 HpaI GTT'AAC Cuts at: 5511 5511 Size: 7921 KpnI G_GTAC'C Cuts at: 2799 5189 5246 2799 Size: 2390 57 5474 Fragments arranged by size: 5474 2390 57 MfeI C'AATT_G Cuts at: 5520 5520 Size: 7921 MluI A'CGCG_T Cuts at: 7797 7797 Size: 7921 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2517 4499 7035 7219 1305 Size: 1212 1982 2536 184 2007 Fragments arranged by size: 2536 2007 1982 1212 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 6099 Fragments arranged by size: 6099 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 3557 3810 4226 5879 6238 6397 518 Size: 648 2391 253 416 1653 359 159 2042 Fragments arranged by size: 2391 2042 1653 648 416 359 253 159 MspA1I CmG'CkG Cuts at: 1606 2155 2178 2275 2533 3101 3969 4441 4701 Size: 549 23 97 258 568 868 472 260 Cuts at: 4701 4880 4979 5337 6014 7162 7407 1606 Size: 179 99 358 677 1148 245 2120 Fragments arranged by size: 2120 1148 868 677 568 549 472 358 260 258 245 179 99 97 23 NaeI GCC'GGC Cuts at: 4990 4990 Size: 7921 NcoI C'CATG_G Cuts at: 513 1065 2737 513 Size: 552 1672 5697 Fragments arranged by size: 5697 1672 552 NdeI CA'TA_TG Cuts at: 387 3119 387 Size: 2732 5189 NgoMIV G'CCGG_C Cuts at: 4988 4988 Size: 7921 NheI G'CTAG_C Cuts at: 1052 2134 1052 Size: 1082 6839 NotI GC'GGCC_GC Cuts at: 5368 5368 Size: 7921 NruI TCG'CGA Cuts at: 4314 4314 Size: 7921 NspI r_CATG'y Cuts at: 1132 1229 3335 4663 5279 6824 1132 Size: 97 2106 1328 616 1545 2229 Fragments arranged by size: 2229 2106 1545 1328 616 97 PasI CC'CwG_GG Cuts at: 1348 2863 3635 4576 1348 Size: 1515 772 941 4693 Fragments arranged by size: 4693 1515 941 772 PciI A'CATG_T Cuts at: 6820 6820 Size: 7921 PflMI CCAn_nnn'nTGG Cuts at: 1108 5047 5125 1108 Size: 3939 78 3904 Fragments arranged by size: 3939 3904 78 PfoI T'CCnGG_A Cuts at: 2238 3489 3597 2238 Size: 1251 108 6562 Fragments arranged by size: 6562 1251 108 PmeI GTTT'AAAC Cuts at: 5225 5225 Size: 7921 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 5990 6022 7503 7535 5990 Size: 32 1481 32 6376 Fragments arranged by size: 6376 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2785 3017 3368 3631 4381 2785 Size: 232 351 263 750 6325 Fragments arranged by size: 6325 750 351 263 232 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 7921 PsiI TTA'TAA Cuts at: 5491 5491 Size: 7921 PspOMI G'GGCC_C Cuts at: 3383 3825 4116 4884 3383 Size: 442 291 768 6420 Fragments arranged by size: 6420 768 442 291 PspXI vC'TCGA_Gb Cuts at: 3271 3271 Size: 7921 PstI C_TGCA'G Cuts at: 830 2154 2568 2793 3543 3570 4005 4700 4754 Size: 1324 414 225 750 27 435 695 54 Cuts at: 4754 5273 830 Size: 519 3478 Fragments arranged by size: 3478 1324 750 695 519 435 414 225 54 27 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 7921 PvuII CAG'CTG Cuts at: 2155 2533 3101 3969 4441 4880 4979 2155 Size: 378 568 868 472 439 99 5097 Fragments arranged by size: 5097 868 568 472 439 378 99 SacI G_AGCT'C Cuts at: 2484 3753 2484 Size: 1269 6652 SalI G'TCGA_C Cuts at: 1936 2845 5263 1936 Size: 909 2418 4594 Fragments arranged by size: 4594 2418 909 SanDI GG'GwC_CC Cuts at: 3017 3368 4381 3017 Size: 351 1013 6557 Fragments arranged by size: 6557 1013 351 SapI GCTCTTCn'nnn_ Cuts at: 5070 5070 Size: 7921 SbfI CC_TGCA'GG Cuts at: 2793 5273 2793 Size: 2480 5441 ScaI AGT'ACT Cuts at: 2463 3545 6169 7881 2463 Size: 1082 2624 1712 2503 Fragments arranged by size: 2624 2503 1712 1082 SexAI A'CCwGG_T Cuts at: 2436 2436 Size: 7921 SfcI C'TryA_G Cuts at: 826 1045 2150 2445 2564 2789 3539 3566 3839 Size: 219 1105 295 119 225 750 27 273 Cuts at: 3839 4001 4696 4750 4841 5269 6404 7085 7276 Size: 162 695 54 91 428 1135 681 191 Cuts at: 7276 826 Size: 1471 Fragments arranged by size: 1471 1135 1105 750 695 681 428 295 273 225 219 191 162 119 91 54 27 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 7921 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 7921 SmaI CCC'GGG Cuts at: 1872 4889 5248 1872 Size: 3017 359 4545 Fragments arranged by size: 4545 3017 359 SmlI C'TyrA_G Cuts at: 820 1017 1943 3271 4785 6025 6926 7188 820 Size: 197 926 1328 1514 1240 901 262 1553 Fragments arranged by size: 1553 1514 1328 1240 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 5712 493 Size: 5219 2702 SpeI A'CTAG_T Cuts at: 152 3308 152 Size: 3156 4765 SphI G_CATG'C Cuts at: 1132 4663 5279 1132 Size: 3531 616 3774 Fragments arranged by size: 3774 3531 616 SrfI GCCC'GGGC Cuts at: 4889 4889 Size: 7921 SspI AAT'ATT Cuts at: 5 52 5845 5 Size: 47 5793 2081 Fragments arranged by size: 5793 2081 47 StuI AGG'CCT Cuts at: 1517 3036 3468 1517 Size: 1519 432 5970 Fragments arranged by size: 5970 1519 432 StyI C'CwwG_G Cuts at: 513 1065 2737 2948 3022 5190 5360 513 Size: 552 1672 211 74 2168 170 3074 Fragments arranged by size: 3074 2168 1672 552 211 170 74 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2760 3274 3632 4027 4376 5948 5965 Size: 1255 1180 514 358 395 349 1572 17 Cuts at: 5965 6118 6303 6748 325 Size: 153 185 445 1498 Fragments arranged by size: 1572 1498 1255 1180 514 445 395 358 349 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2461 3543 6167 Size: 274 80 33 51 1443 484 1082 2624 Cuts at: 6167 7879 96 Size: 1712 138 Fragments arranged by size: 2624 1712 1443 1082 484 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2157 2189 2613 2632 2645 2664 2678 2710 3801 Size: 32 424 19 13 19 14 32 1091 Cuts at: 3801 3833 4153 4185 7881 7913 2157 Size: 32 320 32 3696 32 2165 Fragments arranged by size: 3696 2165 1091 424 320 32 32 32 32 32 19 19 14 13 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 7921 XbaI T'CTAG_A Cuts at: 5257 5257 Size: 7921 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2735 2777 3157 4412 5126 2735 Size: 42 380 1255 714 5530 Fragments arranged by size: 5530 1255 714 380 42 XhoI C'TCGA_G Cuts at: 1943 3271 4785 1943 Size: 1328 1514 5079 Fragments arranged by size: 5079 1514 1328 XmaI C'CCGG_G Cuts at: 1870 4887 5246 1870 Size: 3017 359 4545 Fragments arranged by size: 4545 3017 359 XmnI GAAnn'nnTTC Cuts at: 6050 6050 Size: 7921 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 5729 276 Size: 53 83 186 936 4195 2468 Fragments arranged by size: 4195 2468 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BaeI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsiWI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NruI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI PspXI PstI PvuI PvuII SacI SalI SanDI SapI SbfI ScaI SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SrfI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AscI AvrII BbeI BfrBI BstZ17I DraIII EcoRV FseI FspAI KasI NarI NsiI PacI PmlI PsrI RsrII SacII SfiI SfoI SwaI