Restriction map for
pFN21AB7005
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# NO CUT ENZYMES ( 64 enzymes) #
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Acc65I AclI AflII Aor51HI ApaI
AscI AseI AsnI Asp718I BalI
BamHI BbeI BcgI BfrI BglI
BsiWI BsmBI BspHI BspLU11I BstBI
CpoI DrdI Eco47III EcoRI EcoRV
HaeII HpaI KasI KpnI KspI
MfeI MluI MluNI MscI MunI
NarI NdeI NheI NotI NruI
NspV PacI PaeR7I PmeI PshBI
Psp1406I PvuI RcaI RsrII SacI
SacII SbfI ScaI SfiI SfoI
SfuI SmaI SnaBI SphI Sse8387I
SwaI XhoI XmaCI XmaI
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# ONE CUT ENZYMES ( 53 enzymes) #
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AccIII 2561 TCCGGA T^CCGGA
AflIII 2336 ACGTGT A^C[GA][CT]GT
AgeI 2944 ACCGGT A^CCGGT
AhdI 3559 GACAGCCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Alw44I 942 GTGCAC G^TGCAC
ApaLI 942 GTGCAC G^TGCAC
Asp700I 537 GAATGCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspEI 3559 GACAGCCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AviII 917 TGCGCA TGC^GCA
AvrII 591 CCTAGG C^CTAGG
BciVI 1996 GTATCC GTATCC(6/5)
BclI 3254 TGATCA T^GATCA
BlnI 591 CCTAGG C^CTAGG
BsaBI 2926 GATAGTTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaI 1978 GAGACC GGTCTC(1/5)
BseAI 2561 TCCGGA T^CCGGA
BsiEI 3689 CGGCCG CG[GA][CT]^CG
Bsp1407I 1835 TGTACA T^GTACA
BspDI 882 ATCGAT AT^CGAT
BspEI 2561 TCCGGA T^CCGGA
BsrBI 3451 CCGCTC CCGCTC(-3/-3)
BsrGI 1835 TGTACA T^GTACA
BssHII 987 GCGCGC G^CGCGC
BstAPI 3678 GCAGGCACTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstEII 2751 GGTTACC G^GT[ACGT]ACC
BstPI 2751 GGTTACC G^GT[ACGT]ACC
ClaI 882 ATCGAT AT^CGAT
DraI 3148 TTTAAA TTT^AAA
EagI 3689 CGGCCG C^GGCCG
Eam1105I 3559 GACAGCCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EclXI 3689 CGGCCG C^GGCCG
Eco52I 3689 CGGCCG C^GGCCG
EcoO65I 2751 GGTTACC G^GT[ACGT]ACC
EcoT22I 1744 ATGCAT ATGCA^T
FbaI 3254 TGATCA T^GATCA
FseI 3690 GGCCGGCC GGCCGG^CC
FspI 917 TGCGCA TGC^GCA
MamI 2926 GATAGTTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MroI 2561 TCCGGA T^CCGGA
NcoI 41 CCATGG C^CATGG
NsiI 1744 ATGCAT ATGCA^T
PinAI 2944 ACCGGT A^CCGGT
PshAI 1211 GACTTGTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
SalI 3349 GTCGAC G^TCGAC
SexAI 1050 ACCTGGT A^CC[AT]GGT
SgrAI 26 CGCCGGTG C[GA]^CCGG[CT]G
SpeI 2967 ACTAGT A^CTAGT
SspBI 1835 TGTACA T^GTACA
SspI 662 AATATT AAT^ATT
StuI 3657 AGGCCT AGG^CCT
XbaI 2133 TCTAGA T^CTAGA
XcmI 823 CCAGTGAAGAGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmnI 537 GAATGCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 22 enzymes) #
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AatII 1947 GACGTC GACGT^C
3481 GACGTC
AocI 2144 CCTCAGG CC^T[ACGT]AGG
2858 CCTCAGG
AspI 2578 GACAAGGTC GAC[ACGT]^[ACGT][ACGT]GTC
3751 GACCACGTC
BanI 793 GGTGCC G^G[CT][GA]CC
2167 GGTGCC
BbvCI 1531 GCTGAGG CCTCAGC(-5/-2)
2178 GCTGAGG
BlpI 1282 GCTGAGC GC^T[ACGT]AGC
2466 GCTTAGC
Bpu1102I 1282 GCTGAGC GC^T[ACGT]AGC
2466 GCTTAGC
Bsu36I 2144 CCTCAGG CC^T[ACGT]AGG
2858 CCTCAGG
CelII 1282 GCTGAGC GC^T[ACGT]AGC
2466 GCTTAGC
DraII 148 AGGTCCT [GA]G^G[ACGT]CC[CT]
1227 AGGACCT
Eco81I 2144 CCTCAGG CC^T[ACGT]AGG
2858 CCTCAGG
EcoO109I 148 AGGTCCT [GA]G^G[ACGT]CC[CT]
1227 AGGACCT
FauI 2014 CCCGC CCCGC(4/6)
876 GCGGG
HindIII 1355 AAGCTT A^AGCTT
3331 AAGCTT
NaeI 3687 GCCGGC GCC^GGC
3691 GCCGGC
NgoMIV 3687 GCCGGC G^CCGGC
3691 GCCGGC
NspI 2099 ACATGC [GA]CATG^[CT]
2687 ACATGC
PflFI 2578 GACAAGGTC GAC[ACGT]^[ACGT][ACGT]GTC
3751 GACCACGTC
PflMI 1149 CCACACTGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
3753 CCACGTCCTGG
PpuMI 148 AGGTCCT [GA]G^G[AT]CC[CT]
1227 AGGACCT
Tth111I 2578 GACAAGGTC GAC[ACGT]^[ACGT][ACGT]GTC
3751 GACCACGTC
Van91I 1149 CCACACTGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
3753 CCACGTCCTGG
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# 3 OR MORE CUT ENZYMES ( 119 enzymes) #
#########################################
AccI AccII AciI AcsI AcyI
AfaI AluI AlwI AlwNI ApoI
AspHI AvaI AvaII BanII BbrPI
BbsI BbvI BcnI BfaI BglII
BmyI BpmI BpuAI BsaAI BsaHI
BsaJI BsaWI BseRI BsgI BsiHKAI
BsiYI BslI BsmAI BsmFI BsmI
BsoBI Bsp1286I BspMI BsrDI BsrFI
BsrI BssKI BssSI Bst1107I BstF5I
BstNI BstUI BstXI BstYI BstZ17I
Cac8I CfoI Cfr10I Cfr13I DdeI
DpnII DraIII DsaI EaeI EarI
EcoNI EcoRII EcoT14I Fnu4HI FokI
HaeIII HapII HgaI HhaI Hin1I
HinP1I HincII HindII HinfI HpaII
HphI ItaI Ksp632I MaeI MaeII
MaeIII MboI MboII MflI MnlI
MseI MslI MspA1I MspI MvaI
MvnI MwoI NciI NdeII NlaIII
NlaIV PleI PmaCI PmlI PstI
PvuII RsaI SapI Sau3AI Sau96I
ScrFI SfaNI SfcI SmlI StyI
TaqI TfiI Tru9I TseI Tsp45I
Tsp509I TspRI TthHB8I XhoII