(Circular) (Six-base) MAPSORT of: pFN21AB7004.seq Check: 9784 from: 1 to: 8725 pFN21AB7004 8725 bp With 182 enzymes: SgfI * January 16, 2009 15:25 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2293 2293 Size: 8725 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 6535 278 Size: 53 83 186 936 4999 2468 Fragments arranged by size: 4999 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 6068 1937 Size: 4131 4594 Acc65I G'GTAC_C Cuts at: 5134 6046 5134 Size: 912 7813 AclI AA'CG_TT Cuts at: 1563 6852 7225 1563 Size: 5289 373 3063 Fragments arranged by size: 5289 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2739 3687 4391 4589 6788 8172 8440 1632 Size: 1107 948 704 198 2199 1384 268 1917 Fragments arranged by size: 2199 1917 1384 1107 948 704 268 198 AfeI AGC'GCT Cuts at: 2864 2864 Size: 8725 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 8528 AflIII A'CryG_T Cuts at: 1203 2316 7624 8601 1203 Size: 1113 5308 977 1327 Fragments arranged by size: 5308 1327 1113 977 AgeI A'CCGG_T Cuts at: 7556 7556 Size: 8725 AhdI GACnn_n'nnGTC Cuts at: 7454 7454 Size: 8725 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 8725 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 4361 5338 1184 Size: 3177 977 4571 Fragments arranged by size: 4571 3177 977 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 4418 4450 4418 Size: 32 8693 AlwNI CAG_nnn'CTG Cuts at: 2354 3688 4484 5491 5804 6468 8040 2354 Size: 1334 796 1007 313 664 1572 3039 Fragments arranged by size: 3039 1572 1334 1007 796 664 313 ApaI G_GGCC'C Cuts at: 2697 3048 3836 3890 4013 4124 4200 4478 2697 Size: 351 788 54 123 111 76 278 6944 Fragments arranged by size: 6944 788 351 278 123 111 76 54 ApaLI G'TGCA_C Cuts at: 6782 7938 6782 Size: 1156 7569 ApoI r'AATT_y Cuts at: 1493 2138 2521 2918 3592 3870 6034 6260 6412 Size: 645 383 397 674 278 2164 226 152 Cuts at: 6412 8521 1493 Size: 2109 1697 Fragments arranged by size: 2164 2109 1697 674 645 397 383 278 226 152 AseI AT'TA_AT Cuts at: 160 1032 7279 160 Size: 872 6247 1606 Fragments arranged by size: 6247 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 8725 AvaI C'yCGr_G Cuts at: 1870 1943 3182 3303 3418 4420 5339 5505 5952 Size: 73 1239 121 115 1002 919 166 447 Cuts at: 5952 6050 1870 Size: 98 4545 Fragments arranged by size: 4545 1239 1002 919 447 166 121 115 98 73 AvrII C'CTAG_G Cuts at: 3028 3028 Size: 8725 BamHI G'GATC_C Cuts at: 3962 4617 6055 6422 3962 Size: 655 1438 367 6265 Fragments arranged by size: 6265 1438 655 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2566 3361 4075 5134 6046 Size: 325 217 627 779 795 714 1059 912 Cuts at: 6046 7501 618 Size: 1455 1842 Fragments arranged by size: 1842 1455 1059 912 795 779 714 627 325 217 BanII G_rGCy'C Cuts at: 1391 2036 2280 2697 2805 3048 3067 3836 3890 Size: 645 244 417 108 243 19 769 54 Cuts at: 3890 4013 4124 4200 4415 4478 5850 5876 6044 Size: 123 111 76 215 63 1372 26 168 Cuts at: 6044 1391 Size: 4072 Fragments arranged by size: 4072 1372 769 645 417 244 243 215 168 123 111 108 76 63 54 26 19 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2232 2300 4733 928 Size: 971 333 68 2433 4920 Fragments arranged by size: 4920 2433 971 333 68 BbvCI CC'TCA_GC Cuts at: 4976 4976 Size: 8725 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 1987 2021 3284 3318 6916 6950 1903 Size: 34 50 34 1263 34 3598 34 3678 Fragments arranged by size: 3678 3598 1263 50 34 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 6617 7827 6617 Size: 1210 7515 BclI T'GATC_A Cuts at: 1548 1548 Size: 8725 BfrBI ATG'CAT Cuts at: 2008 2008 Size: 8725 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 3011 4041 5277 Size: 107 122 71 631 605 1339 1030 1236 Cuts at: 5277 5845 7336 136 Size: 568 1491 1525 Fragments arranged by size: 1525 1491 1339 1236 1030 631 605 568 122 107 71 BglII A'GATC_T Cuts at: 5401 8720 5401 Size: 3319 5406 BlpI GC'TnA_GC Cuts at: 4062 6142 4062 Size: 2080 6645 Bme1580I G_kGCm'C Cuts at: 1790 2697 3048 3364 3836 3890 4013 4124 4200 Size: 907 351 316 472 54 123 111 76 Cuts at: 4200 4478 6786 7942 1790 Size: 278 2308 1156 2573 Fragments arranged by size: 2573 2308 1156 907 472 351 316 278 123 111 76 54 BmgBI CAC'GTC Cuts at: 1318 2597 3058 5971 1318 Size: 1279 461 2913 4072 Fragments arranged by size: 4072 2913 1279 461 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2700 2822 3133 Size: 416 53 83 399 24 1283 122 311 Cuts at: 3133 4833 5214 6456 7414 442 Size: 1700 381 1242 958 1753 Fragments arranged by size: 1753 1700 1283 1242 958 416 399 381 311 122 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 8725 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2052 2052 Size: 8725 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2283 2870 3090 3257 3567 4272 4755 4887 Size: 1044 587 220 167 310 705 483 132 Cuts at: 4887 5157 5352 5445 5862 7385 1239 Size: 270 195 93 417 1523 2579 Fragments arranged by size: 2579 1523 1044 705 587 483 417 310 270 220 195 167 132 93 Bpu10I CC'TnA_GC Cuts at: 4976 4976 Size: 8725 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3063 3923 4415 4529 5124 6850 7715 8013 3063 Size: 860 492 114 595 1726 865 298 3775 Fragments arranged by size: 3775 1726 865 860 595 492 298 114 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 3713 5689 7388 882 Size: 617 2214 1976 1699 2219 Fragments arranged by size: 2219 2214 1976 1699 617 BsaAI yAC'GTr Cuts at: 493 1204 2317 6516 493 Size: 711 1113 4199 2702 Fragments arranged by size: 4199 2702 1113 711 BsaBI GATnn'nnATC Cuts at: 3942 8719 3942 Size: 4777 3948 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 6532 6914 7549 275 Size: 53 83 186 936 4999 382 635 1451 Fragments arranged by size: 4999 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 5432 5644 7158 7556 7830 7977 8388 5432 Size: 212 1514 398 274 147 411 5769 Fragments arranged by size: 5769 1514 411 398 274 212 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 4270 4300 4573 4603 4753 Size: 30 165 30 2867 30 273 30 150 Cuts at: 4753 4783 1178 Size: 30 5120 Fragments arranged by size: 5120 2867 273 165 150 30 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2044 3247 4031 4275 5046 1186 Size: 858 1203 784 244 771 4865 Fragments arranged by size: 4865 1203 858 784 771 244 BseYI C'CCAG_C Cuts at: 2124 2190 2497 3357 3703 3743 3777 4001 4101 Size: 66 307 860 346 40 34 224 100 Cuts at: 4101 4408 4477 4657 4666 4995 5285 5480 7928 Size: 307 69 180 9 329 290 195 2448 Cuts at: 7928 8424 2124 Size: 496 2425 Fragments arranged by size: 2448 2425 860 496 346 329 307 307 290 224 195 180 100 69 66 40 34 9 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 8152 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 6175 6936 7964 1540 Size: 275 186 4174 761 1028 2301 Fragments arranged by size: 4174 2301 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2036 2856 2932 3067 4098 6786 6871 7942 2036 Size: 820 76 135 1031 2688 85 1071 2819 Fragments arranged by size: 2819 2688 1071 1031 820 135 85 76 BsmI GAATG_Cn' Cuts at: 2008 4348 6236 6329 2008 Size: 2340 1888 93 4404 Fragments arranged by size: 4404 2340 1888 93 BsmBI CGTCTCn'nnnn_ Cuts at: 2389 2389 Size: 8725 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2036 2280 2697 2805 2856 2932 3048 Size: 399 246 244 417 108 51 76 116 Cuts at: 3048 3067 3364 3836 3890 4013 4098 4124 4200 Size: 19 297 472 54 123 85 26 76 Cuts at: 4200 4415 4478 5850 5876 6044 6786 6871 7942 Size: 215 63 1372 26 168 742 85 1071 Cuts at: 7942 1391 Size: 2174 Fragments arranged by size: 2174 1372 1071 742 472 417 399 297 246 244 215 168 123 116 108 85 85 76 76 63 54 51 26 26 19 BspEI T'CCGG_A Cuts at: 5644 8388 5644 Size: 2744 5981 BspHI T'CATG_A Cuts at: 5928 6614 8344 5928 Size: 686 1730 6309 Fragments arranged by size: 6309 1730 686 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2293 5185 5301 6080 844 Size: 1449 2892 116 779 3489 Fragments arranged by size: 3489 2892 1449 779 116 BsrBI CCG'CTC Cuts at: 1394 1823 6612 1394 Size: 429 4789 3507 Fragments arranged by size: 4789 3507 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4839 7220 7394 65 Size: 1183 3591 2381 174 1396 Fragments arranged by size: 3591 2381 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4218 7369 7556 1700 Size: 110 2408 3151 187 2869 Fragments arranged by size: 3151 2869 2408 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 8725 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 8725 BssSI C'ACGA_G Cuts at: 6785 7797 6785 Size: 1012 7713 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 8725 BstBI TT'CG_AA Cuts at: 7529 7529 Size: 8725 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 8725 BstXI CCAn_nnnn'nTGG Cuts at: 5543 5999 5543 Size: 456 8269 BstYI r'GATC_y Cuts at: 1972 2761 3962 4617 5401 6055 6422 6807 6824 Size: 789 1201 655 784 654 367 385 17 Cuts at: 6824 8276 8362 8374 8720 1972 Size: 1452 86 12 346 1977 Fragments arranged by size: 1977 1452 1201 789 784 655 654 385 367 346 86 17 12 Bsu36I CC'TnA_GG Cuts at: 4014 4014 Size: 8725 BtgI C'CryG_G Cuts at: 513 1065 2254 3726 4647 4956 5037 5808 513 Size: 552 1189 1472 921 309 81 771 3430 Fragments arranged by size: 3430 1472 1189 921 771 552 309 81 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 4434 487 Size: 683 56 3208 4778 Fragments arranged by size: 4778 3208 683 56 BtsI GCAGTG_nn' Cuts at: 2672 2727 3269 4079 6241 7035 7055 2672 Size: 55 542 810 2162 794 20 4342 Fragments arranged by size: 4342 2162 810 794 542 55 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 8725 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 4938 4973 5019 5054 529 Size: 35 4374 35 46 35 4200 Fragments arranged by size: 4374 4200 46 35 35 35 DraI TTT'AAA Cuts at: 6029 6029 Size: 8725 DraIII CAC_nnn'GTG Cuts at: 2936 2936 Size: 8725 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 3284 7732 809 Size: 507 1968 4448 1802 Fragments arranged by size: 4448 1968 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2257 2334 2505 Size: 54 1425 50 275 18 427 77 171 Cuts at: 2505 3557 3604 3628 3723 4878 5278 5773 6172 Size: 1052 47 24 95 1155 400 495 399 Cuts at: 6172 7061 8690 8 Size: 889 1629 43 Fragments arranged by size: 1629 1425 1155 1052 889 495 427 400 399 275 171 95 77 54 50 47 43 24 18 EagI C'GGCC_G Cuts at: 1537 1812 6172 1537 Size: 275 4360 4090 Fragments arranged by size: 4360 4090 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2758 3219 3224 3663 4591 6655 1353 Size: 1405 461 5 439 928 2064 3423 Fragments arranged by size: 3423 2064 1405 928 461 439 5 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2766 4324 7312 7686 7832 387 Size: 1064 138 1177 1558 2988 374 146 1280 Fragments arranged by size: 2988 1558 1280 1177 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2034 3065 2034 Size: 1031 7694 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2283 2739 2870 3090 3257 3567 3687 Size: 393 651 456 131 220 167 310 120 Cuts at: 3687 4272 4391 4589 4755 4887 5157 5352 5445 Size: 585 119 198 166 132 270 195 93 Cuts at: 5445 5862 6788 7385 8172 8440 1239 Size: 417 926 597 787 268 1524 Fragments arranged by size: 1524 926 787 651 597 585 456 417 393 310 270 268 220 198 195 167 166 132 131 120 119 93 EcoNI CCTnn'n_nnAGG Cuts at: 2646 2837 3032 3351 3630 2646 Size: 191 195 319 279 7741 Fragments arranged by size: 7741 319 279 195 191 EcoO109I rG'GnC_Cy Cuts at: 3038 3045 3155 3320 3832 3833 3887 4010 4121 Size: 7 110 165 512 1 54 123 111 Cuts at: 4121 5042 5065 5075 5115 5270 5336 5345 5540 Size: 921 23 10 40 155 66 9 195 Cuts at: 5540 5729 5837 5912 5949 5996 3038 Size: 189 108 75 37 47 5767 Fragments arranged by size: 5767 921 512 195 189 165 155 123 111 110 108 75 66 54 47 40 37 23 10 9 7 1 EcoRI G'AATT_C Cuts at: 2521 2918 3870 6034 6412 2521 Size: 397 952 2164 378 4834 Fragments arranged by size: 4834 2164 952 397 378 EcoRV GAT'ATC Cuts at: 2454 2887 2454 Size: 433 8292 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 5291 5291 Size: 8725 FspI TGC'GCA Cuts at: 7231 7231 Size: 8725 HaeII r_GCGC'y Cuts at: 1678 2438 2866 3784 7872 1678 Size: 760 428 918 4088 2531 Fragments arranged by size: 4088 2531 918 760 428 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2074 2106 2819 2851 3274 Size: 32 135 32 502 32 713 32 423 Cuts at: 3274 3306 3967 3999 4573 4605 4880 4912 5011 Size: 32 661 32 574 32 275 32 99 Cuts at: 5011 5043 5122 5154 5716 5748 5779 5811 7366 Size: 32 79 32 562 32 31 32 1555 Cuts at: 7366 7398 7440 7472 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 1555 713 661 574 562 502 423 275 135 99 79 42 32 32 32 32 32 32 32 32 32 32 32 32 32 32 31 HincII GTy'rAC Cuts at: 669 1657 1938 3955 6069 6315 669 Size: 988 281 2017 2114 246 3079 Fragments arranged by size: 3079 2114 2017 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 8725 HpaI GTT'AAC Cuts at: 6315 6315 Size: 8725 KpnI G_GTAC'C Cuts at: 5138 6050 5138 Size: 912 7813 MfeI C'AATT_G Cuts at: 4191 6324 4191 Size: 2133 6592 MluI A'CGCG_T Cuts at: 8601 8601 Size: 8725 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 3990 5443 5737 7839 8023 1305 Size: 2685 1453 294 2102 184 2007 Fragments arranged by size: 2685 2102 2007 1453 294 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2259 2336 3559 3606 3725 Size: 54 1425 343 427 77 1223 47 119 Cuts at: 3725 4880 10 Size: 1155 3855 Fragments arranged by size: 3855 1425 1223 1155 427 343 119 77 54 47 MslI CAynn'nnrTG Cuts at: 518 1166 3090 3752 4576 5786 5888 6683 7042 Size: 648 1924 662 824 1210 102 795 359 Cuts at: 7042 7201 518 Size: 159 2042 Fragments arranged by size: 2042 1924 1210 824 795 662 648 359 159 102 MspA1I CmG'CkG Cuts at: 1606 2128 2848 3621 3688 3703 4456 4481 5801 Size: 522 720 773 67 15 753 25 1320 Cuts at: 5801 6141 6818 7966 8211 1606 Size: 340 677 1148 245 2120 Fragments arranged by size: 2120 1320 1148 773 753 720 677 522 340 245 67 25 15 NaeI GCC'GGC Cuts at: 4220 4220 Size: 8725 NcoI C'CATG_G Cuts at: 513 1065 2254 3726 4647 513 Size: 552 1189 1472 921 4591 Fragments arranged by size: 4591 1472 1189 921 552 NdeI CA'TA_TG Cuts at: 387 3174 4230 387 Size: 2787 1056 4882 Fragments arranged by size: 4882 2787 1056 NgoMIV G'CCGG_C Cuts at: 4218 4218 Size: 8725 NheI G'CTAG_C Cuts at: 1052 1052 Size: 8725 NotI GC'GGCC_GC Cuts at: 6172 6172 Size: 8725 NsiI A_TGCA'T Cuts at: 2010 2010 Size: 8725 NspI r_CATG'y Cuts at: 1132 1229 2899 3403 3448 4054 4468 5380 5566 Size: 97 1670 504 45 606 414 912 186 Cuts at: 5566 6083 7628 1132 Size: 517 1545 2229 Fragments arranged by size: 2229 1670 1545 912 606 517 504 414 186 97 45 PasI CC'CwG_GG Cuts at: 1348 2737 3716 5161 5536 5861 1348 Size: 1389 979 1445 375 325 4212 Fragments arranged by size: 4212 1445 1389 979 375 325 PciI A'CATG_T Cuts at: 7624 7624 Size: 8725 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 8725 PfoI T'CCnGG_A Cuts at: 3273 5542 3273 Size: 2269 6456 PmeI GTTT'AAAC Cuts at: 6029 6029 Size: 8725 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4784 4816 5499 5531 6794 6826 8307 8339 4784 Size: 32 683 32 1263 32 1481 32 5170 Fragments arranged by size: 5170 1481 1263 683 32 32 32 32 PpuMI rG'GwC_Cy Cuts at: 3038 3155 3320 5042 5065 5115 5345 5540 5729 Size: 117 165 1722 23 50 230 195 189 Cuts at: 5729 5912 5996 3038 Size: 183 84 5767 Fragments arranged by size: 5767 1722 230 195 189 183 165 117 84 50 23 PshAI GACnn'nnGTC Cuts at: 1613 2729 1613 Size: 1116 7609 PsiI TTA'TAA Cuts at: 6295 6295 Size: 8725 PspOMI G'GGCC_C Cuts at: 2693 3044 3832 3886 4009 4120 4196 4474 2693 Size: 351 788 54 123 111 76 278 6944 Fragments arranged by size: 6944 788 351 278 123 111 76 54 PspXI vC'TCGA_Gb Cuts at: 5505 5505 Size: 8725 PstI C_TGCA'G Cuts at: 830 2350 2547 3210 5409 6077 830 Size: 1520 197 663 2199 668 3478 Fragments arranged by size: 3478 2199 1520 668 663 197 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 8725 PvuII CAG'CTG Cuts at: 2128 2848 3688 3703 4456 4481 5801 2128 Size: 720 840 15 753 25 1320 5052 Fragments arranged by size: 5052 1320 840 753 720 25 15 SacI G_AGCT'C Cuts at: 2036 3067 2036 Size: 1031 7694 SalI G'TCGA_C Cuts at: 1936 6067 1936 Size: 4131 4594 SanDI GG'GwC_CC Cuts at: 5042 5065 5115 5912 5042 Size: 23 50 797 7855 Fragments arranged by size: 7855 797 50 23 SapI GCTCTTCn'nnn_ Cuts at: 2758 2758 Size: 8725 SbfI CC_TGCA'GG Cuts at: 6077 6077 Size: 8725 ScaI AGT'ACT Cuts at: 6973 8685 6973 Size: 1712 7013 SexAI A'CCwGG_T Cuts at: 3535 3535 Size: 8725 SfcI C'TryA_G Cuts at: 826 1045 2097 2226 2346 2543 2646 3206 4701 Size: 219 1052 129 120 197 103 560 1495 Cuts at: 4701 5405 6073 7208 7889 8080 826 Size: 704 668 1135 681 191 1471 Fragments arranged by size: 1495 1471 1135 1052 704 681 668 560 219 197 191 129 120 103 SfiI GGCCn_nnn'nGGCC Cuts at: 3011 5277 3011 Size: 2266 6459 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 8725 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 8725 SmaI CCC'GGG Cuts at: 1872 5341 5954 6052 1872 Size: 3469 613 98 4545 Fragments arranged by size: 4545 3469 613 98 SmlI C'TyrA_G Cuts at: 820 1017 1943 3078 3902 4430 4544 5139 5505 Size: 197 926 1135 824 528 114 595 366 Cuts at: 5505 6829 7730 7992 820 Size: 1324 901 262 1553 Fragments arranged by size: 1553 1324 1135 926 901 824 595 528 366 262 197 114 SnaBI TAC'GTA Cuts at: 493 6516 493 Size: 6023 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 8725 SphI G_CATG'C Cuts at: 1132 2899 4468 5380 6083 1132 Size: 1767 1569 912 703 3774 Fragments arranged by size: 3774 1767 1569 912 703 SspI AAT'ATT Cuts at: 5 52 5498 6649 5 Size: 47 5446 1151 2081 Fragments arranged by size: 5446 2081 1151 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 8725 StyI C'CwwG_G Cuts at: 513 1065 2254 3028 3655 3726 4317 4647 5275 Size: 552 1189 774 627 71 591 330 628 Cuts at: 5275 5992 6164 513 Size: 717 172 3074 Fragments arranged by size: 3074 1189 774 717 628 627 591 552 330 172 71 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2572 2590 5870 5969 6752 6769 6922 Size: 1255 992 18 3280 99 783 17 153 Cuts at: 6922 7107 7552 325 Size: 185 445 1498 Fragments arranged by size: 3280 1498 1255 992 783 445 185 153 99 18 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 4751 5176 6971 Size: 274 80 33 51 1443 2774 425 1795 Cuts at: 6971 8683 96 Size: 1712 138 Fragments arranged by size: 2774 1795 1712 1443 425 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2162 2194 4270 4302 8685 8717 2162 Size: 32 2076 32 4383 32 2170 Fragments arranged by size: 4383 2170 2076 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 3537 1316 Size: 2221 6504 XbaI T'CTAG_A Cuts at: 3530 6061 3530 Size: 2531 6194 XcmI CCAnnnn_n'nnnnTGG Cuts at: 3791 4109 3791 Size: 318 8407 XhoI C'TCGA_G Cuts at: 1943 5505 1943 Size: 3562 5163 XmaI C'CCGG_G Cuts at: 1870 5339 5952 6050 1870 Size: 3469 613 98 4545 Fragments arranged by size: 4545 3469 613 98 XmnI GAAnn'nnTTC Cuts at: 2918 3117 6854 2918 Size: 199 3737 4789 Fragments arranged by size: 4789 3737 199 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 6533 276 Size: 53 83 186 936 4999 2468 Fragments arranged by size: 4999 2468 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI AvrII BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI EcoRV FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NsiI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI PspXI PstI PvuI PvuII SacI SalI SanDI SapI SbfI ScaI SexAI SfcI SfiI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AscI BaeI BbeI BsiWI BstZ17I FseI FspAI KasI NarI NruI PacI PmlI PsrI RsrII SacII SfoI SrfI SwaI