(Circular) (Six-base) MAPSORT of: pFN21AB7003.seq Check: 5910 from: 1 to: 8566 pFN21AB7003 8566 bp With 182 enzymes: SgfI * January 16, 2009 15:25 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 3538 3804 4403 3538 Size: 266 599 7701 Fragments arranged by size: 7701 599 266 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 6376 278 Size: 53 83 186 936 4840 2468 Fragments arranged by size: 4840 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 4883 5909 1937 Size: 2946 1026 4594 Fragments arranged by size: 4594 2946 1026 Acc65I G'GTAC_C Cuts at: 2068 5887 2068 Size: 3819 4747 AclI AA'CG_TT Cuts at: 1563 3807 4929 6693 7066 1563 Size: 2244 1122 1764 373 3063 Fragments arranged by size: 3063 2244 1764 1122 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2425 3749 3927 4196 6629 8013 8281 1632 Size: 793 1324 178 269 2433 1384 268 1917 Fragments arranged by size: 2433 1917 1384 1324 793 269 268 178 AfeI AGC'GCT Cuts at: 3073 3073 Size: 8566 AflII C'TTAA_G Cuts at: 820 1017 5000 5161 820 Size: 197 3983 161 4225 Fragments arranged by size: 4225 3983 197 161 AflIII A'CryG_T Cuts at: 1203 5346 7465 8442 1203 Size: 4143 2119 977 1327 Fragments arranged by size: 4143 2119 1327 977 AgeI A'CCGG_T Cuts at: 7397 7397 Size: 8566 AhdI GACnn_n'nnGTC Cuts at: 7295 7295 Size: 8566 AleI CACnn'nnGTG Cuts at: 1166 2612 3939 5045 5079 1166 Size: 1446 1327 1106 34 4653 Fragments arranged by size: 4653 1446 1327 1106 34 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 8566 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5462 5494 5462 Size: 32 8534 AlwNI CAG_nnn'CTG Cuts at: 2891 3278 3629 4249 6309 7881 2891 Size: 387 351 620 2060 1572 3576 Fragments arranged by size: 3576 2060 1572 620 387 351 ApaLI G'TGCA_C Cuts at: 2097 6623 7779 2097 Size: 4526 1156 2884 Fragments arranged by size: 4526 2884 1156 ApoI r'AATT_y Cuts at: 1493 2227 2558 2787 2896 3121 3856 4461 4480 Size: 734 331 229 109 225 735 605 19 Cuts at: 4480 4592 4812 5809 5875 6101 6253 8362 1493 Size: 112 220 997 66 226 152 2109 1697 Fragments arranged by size: 2109 1697 997 735 734 605 331 229 226 225 220 152 112 109 66 19 AseI AT'TA_AT Cuts at: 160 1032 4497 5298 5538 7120 160 Size: 872 3465 801 240 1582 1606 Fragments arranged by size: 3465 1606 1582 872 801 240 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 8566 AvaI C'yCGr_G Cuts at: 1870 1943 5891 1870 Size: 73 3948 4545 Fragments arranged by size: 4545 3948 73 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 5568 5601 5568 Size: 33 8533 BamHI G'GATC_C Cuts at: 3884 5896 6263 3884 Size: 2012 367 6187 Fragments arranged by size: 6187 2012 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2068 4724 5819 5887 7342 Size: 325 217 627 281 2656 1095 68 1455 Cuts at: 7342 618 Size: 1842 Fragments arranged by size: 2656 1842 1455 1095 627 325 281 217 68 BanII G_rGCy'C Cuts at: 1391 5885 1391 Size: 4494 4072 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2189 3132 3684 3757 4290 4309 4329 Size: 971 290 943 552 73 533 19 20 Cuts at: 4329 4508 928 Size: 179 4986 Fragments arranged by size: 4986 971 943 552 533 290 179 73 20 19 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 6757 6791 1903 Size: 34 4820 34 3678 Fragments arranged by size: 4820 3678 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3666 4624 6458 7668 3666 Size: 958 1834 1210 4564 Fragments arranged by size: 4564 1834 1210 958 BclI T'GATC_A Cuts at: 1548 3178 1548 Size: 1630 6936 BfrBI ATG'CAT Cuts at: 3968 4169 5094 3968 Size: 201 925 7440 Fragments arranged by size: 7440 925 201 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 7177 136 Size: 107 122 71 631 605 5505 1525 Fragments arranged by size: 5505 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 8561 8561 Size: 8566 BlpI GC'TnA_GC Cuts at: 2211 5983 2211 Size: 3772 4794 Bme1580I G_kGCm'C Cuts at: 1790 2101 6627 7783 1790 Size: 311 4526 1156 2573 Fragments arranged by size: 4526 2573 1156 311 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 8566 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 4551 6297 7255 Size: 416 53 83 399 24 3134 1746 958 Cuts at: 7255 442 Size: 1753 Fragments arranged by size: 3134 1753 1746 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 4395 1056 Size: 3339 5227 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4450 4450 Size: 8566 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2142 3465 4146 7226 1239 Size: 903 1323 681 3080 2579 Fragments arranged by size: 3080 2579 1323 903 681 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2379 2664 2677 3478 4018 6691 7556 7854 2379 Size: 285 13 801 540 2673 865 298 3091 Fragments arranged by size: 3091 2673 865 801 540 298 285 13 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 3994 7229 882 Size: 617 2495 3235 2219 Fragments arranged by size: 3235 2495 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 6357 493 Size: 711 5153 2702 Fragments arranged by size: 5153 2702 711 BsaBI GATnn'nnATC Cuts at: 4170 8560 4170 Size: 4390 4176 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 6373 6755 7390 275 Size: 53 83 186 936 4840 382 635 1451 Fragments arranged by size: 4840 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 6999 7397 7671 7818 8229 6999 Size: 398 274 147 411 7336 Fragments arranged by size: 7336 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 8341 Fragments arranged by size: 8341 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 1186 Size: 8566 BseYI C'CCAG_C Cuts at: 3405 7769 8265 3405 Size: 4364 496 3706 Fragments arranged by size: 4364 3706 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 7993 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 5258 6016 6777 7805 1540 Size: 275 186 3257 758 761 1028 2301 Fragments arranged by size: 3257 2301 1028 761 758 275 186 BsiHKAI G_wGCw'C Cuts at: 2101 4385 6627 6712 7783 2101 Size: 2284 2242 85 1071 2884 Fragments arranged by size: 2884 2284 2242 1071 85 BsmI GAATG_Cn' Cuts at: 2333 2954 3922 3968 4411 4916 6077 6170 2333 Size: 621 968 46 443 505 1161 93 4729 Fragments arranged by size: 4729 1161 968 621 505 443 93 46 BsmBI CGTCTCn'nnnn_ Cuts at: 5846 5846 Size: 8566 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2101 4385 5885 6627 6712 7783 1391 Size: 399 311 2284 1500 742 85 1071 2174 Fragments arranged by size: 2284 2174 1500 1071 742 399 311 85 BspEI T'CCGG_A Cuts at: 8229 8229 Size: 8566 BspHI T'CATG_A Cuts at: 3598 4906 6455 8185 3598 Size: 1308 1549 1730 3979 Fragments arranged by size: 3979 1730 1549 1308 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3538 3804 4403 5921 844 Size: 2694 266 599 1518 3489 Fragments arranged by size: 3489 2694 1518 599 266 BsrBI CCG'CTC Cuts at: 1394 1823 5157 6453 1394 Size: 429 3334 1296 3507 Fragments arranged by size: 3507 3334 1296 429 BsrDI GCAATG_nn' Cuts at: 65 1248 3395 4371 4575 7061 7235 65 Size: 1183 2147 976 204 2486 174 1396 Fragments arranged by size: 2486 2147 1396 1183 976 204 174 BsrFI r'CCGG_y Cuts at: 1700 1810 7210 7397 1700 Size: 110 5400 187 2869 Fragments arranged by size: 5400 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 3640 96 Size: 3544 5022 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 8566 BssSI C'ACGA_G Cuts at: 6626 7638 6626 Size: 1012 7554 BstAPI GCAn_nnn'nTGC Cuts at: 794 4110 4774 4783 794 Size: 3316 664 9 4577 Fragments arranged by size: 4577 3316 664 9 BstBI TT'CG_AA Cuts at: 5695 7370 5695 Size: 1675 6891 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 8566 BstXI CCAn_nnnn'nTGG Cuts at: 5086 5176 5086 Size: 90 8476 BstYI r'GATC_y Cuts at: 1972 3261 3884 5896 6263 6648 6665 8117 8203 Size: 1289 623 2012 367 385 17 1452 86 Cuts at: 8203 8215 8561 1972 Size: 12 346 1977 Fragments arranged by size: 2012 1977 1452 1289 623 385 367 346 86 17 12 Bsu36I CC'TnA_GG Cuts at: 2823 4005 5474 2823 Size: 1182 1469 5915 Fragments arranged by size: 5915 1469 1182 BtgI C'CryG_G Cuts at: 513 1065 2004 2715 513 Size: 552 939 711 6364 Fragments arranged by size: 6364 939 711 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 4035 487 Size: 683 56 2809 5018 Fragments arranged by size: 5018 2809 683 56 BtsI GCAGTG_nn' Cuts at: 3508 3782 5115 5404 5691 6082 6876 6896 3508 Size: 274 1333 289 287 391 794 20 5178 Fragments arranged by size: 5178 1333 794 391 289 287 274 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 8566 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 2151 2186 4950 4985 529 Size: 35 1587 35 2764 35 4110 Fragments arranged by size: 4110 2764 1587 35 35 35 DraI TTT'AAA Cuts at: 2454 2602 5220 5870 2454 Size: 148 2618 650 5150 Fragments arranged by size: 5150 2618 650 148 DraIII CAC_nnn'GTG Cuts at: 4110 4110 Size: 8566 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 7573 809 Size: 507 6257 1802 Fragments arranged by size: 6257 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 4672 6013 6902 Size: 54 1425 50 275 18 2842 1341 889 Cuts at: 6902 8531 8 Size: 1629 43 Fragments arranged by size: 2842 1629 1425 1341 889 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 6013 1537 Size: 275 4201 4090 Fragments arranged by size: 4201 4090 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2361 3645 4560 4627 5369 5412 6496 1353 Size: 1008 1284 915 67 742 43 1084 3423 Fragments arranged by size: 3423 1284 1084 1008 915 742 67 43 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 7153 7527 7673 387 Size: 1064 138 5564 374 146 1280 Fragments arranged by size: 5564 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2142 2425 3465 3749 3927 4146 4196 Size: 393 510 283 1040 284 178 219 50 Cuts at: 4196 6629 7226 8013 8281 1239 Size: 2433 597 787 268 1524 Fragments arranged by size: 2433 1524 1040 787 597 510 393 284 283 268 219 178 50 EcoRI G'AATT_C Cuts at: 5809 5875 6253 5809 Size: 66 378 8122 Fragments arranged by size: 8122 378 66 EcoRV GAT'ATC Cuts at: 2708 2708 Size: 8566 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 4086 4086 Size: 8566 FspI TGC'GCA Cuts at: 7072 7072 Size: 8566 HaeII r_GCGC'y Cuts at: 1678 3075 7713 1678 Size: 1397 4638 2531 Fragments arranged by size: 4638 2531 1397 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2115 2147 2281 2313 2511 Size: 32 135 32 543 32 134 32 198 Cuts at: 2511 2543 2650 2682 3170 3202 3760 3792 3887 Size: 32 107 32 488 32 558 32 95 Cuts at: 3887 3919 4865 4897 5370 5402 7207 7239 7281 Size: 32 946 32 473 32 1805 32 42 Cuts at: 7281 7313 1373 Size: 32 2626 Fragments arranged by size: 2626 1805 946 558 543 488 473 198 135 134 107 95 42 32 32 32 32 32 32 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 5637 5910 6156 669 Size: 988 281 3699 273 246 3079 Fragments arranged by size: 3699 3079 988 281 273 246 HindIII A'AGCT_T Cuts at: 748 2165 748 Size: 1417 7149 HpaI GTT'AAC Cuts at: 6156 6156 Size: 8566 KpnI G_GTAC'C Cuts at: 2072 5891 2072 Size: 3819 4747 MfeI C'AATT_G Cuts at: 6165 6165 Size: 8566 MluI A'CGCG_T Cuts at: 8442 8442 Size: 8566 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2215 2660 2727 3276 3289 3862 4215 4641 Size: 910 445 67 549 13 573 353 426 Cuts at: 4641 7680 7864 1305 Size: 3039 184 2007 Fragments arranged by size: 3039 2007 910 573 549 445 426 353 184 67 13 MscI TGG'CCA Cuts at: 10 64 1489 1832 4674 10 Size: 54 1425 343 2842 3902 Fragments arranged by size: 3902 2842 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2092 2612 3939 4376 4580 5045 5079 Size: 648 926 520 1327 437 204 465 34 Cuts at: 5079 6524 6883 7042 518 Size: 1445 359 159 2042 Fragments arranged by size: 2042 1445 1327 926 648 520 465 437 359 204 159 34 MspA1I CmG'CkG Cuts at: 1606 4678 5233 5982 6659 7807 8052 1606 Size: 3072 555 749 677 1148 245 2120 Fragments arranged by size: 3072 2120 1148 749 677 555 245 NcoI C'CATG_G Cuts at: 513 1065 2004 2715 513 Size: 552 939 711 6364 Fragments arranged by size: 6364 939 711 552 NdeI CA'TA_TG Cuts at: 387 4077 387 Size: 3690 4876 NheI G'CTAG_C Cuts at: 1052 4391 1052 Size: 3339 5227 NotI GC'GGCC_GC Cuts at: 6013 6013 Size: 8566 NsiI A_TGCA'T Cuts at: 3970 4171 5096 3970 Size: 201 925 7440 Fragments arranged by size: 7440 925 201 NspI r_CATG'y Cuts at: 1132 1229 4117 4699 4772 5094 5134 5350 5924 Size: 97 2888 582 73 322 40 216 574 Cuts at: 5924 7469 1132 Size: 1545 2229 Fragments arranged by size: 2888 2229 1545 582 574 322 216 97 73 40 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 8566 PciI A'CATG_T Cuts at: 5346 7465 5346 Size: 2119 6447 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 8566 PmeI GTTT'AAAC Cuts at: 5870 5870 Size: 8566 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 6635 6667 8148 8180 6635 Size: 32 1481 32 7021 Fragments arranged by size: 7021 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 8566 PsiI TTA'TAA Cuts at: 2697 6136 2697 Size: 3439 5127 PsrI GAACnnnnnnTACnnnnnnn_nnnnn' Cuts at: 3753 3785 4635 4667 3753 Size: 32 850 32 7652 Fragments arranged by size: 7652 850 32 32 PstI C_TGCA'G Cuts at: 830 5686 5918 830 Size: 4856 232 3478 Fragments arranged by size: 4856 3478 232 PvuI CG_AT'CG Cuts at: 2001 5258 2001 Size: 3257 5309 PvuII CAG'CTG Cuts at: 4678 5233 4678 Size: 555 8011 SalI G'TCGA_C Cuts at: 1936 5908 1936 Size: 3972 4594 SbfI CC_TGCA'GG Cuts at: 5918 5918 Size: 8566 ScaI AGT'ACT Cuts at: 6814 8526 6814 Size: 1712 6854 SexAI A'CCwGG_T Cuts at: 2045 2045 Size: 8566 SfcI C'TryA_G Cuts at: 826 1045 2152 2462 2815 3990 5202 5682 5914 Size: 219 1107 310 353 1175 1212 480 232 Cuts at: 5914 7049 7730 7921 826 Size: 1135 681 191 1471 Fragments arranged by size: 1471 1212 1175 1135 1107 681 480 353 310 232 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 8566 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 8566 SmaI CCC'GGG Cuts at: 1872 5893 1872 Size: 4021 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2394 2656 2679 3457 3997 5000 Size: 197 926 451 262 23 778 540 1003 Cuts at: 5000 5161 6670 7571 7833 820 Size: 161 1509 901 262 1553 Fragments arranged by size: 1553 1509 1003 926 901 778 540 451 262 262 197 161 23 SnaBI TAC'GTA Cuts at: 493 6357 493 Size: 5864 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 8566 SphI G_CATG'C Cuts at: 1132 4117 5094 5924 1132 Size: 2985 977 830 3774 Fragments arranged by size: 3774 2985 977 830 SspI AAT'ATT Cuts at: 5 52 2674 4042 5216 5456 5596 6490 5 Size: 47 2622 1368 1174 240 140 894 2081 Fragments arranged by size: 2622 2081 1368 1174 894 240 140 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 8566 StyI C'CwwG_G Cuts at: 513 1065 2004 2490 2715 2760 4863 5017 6005 Size: 552 939 486 225 45 2103 154 988 Cuts at: 6005 513 Size: 3074 Fragments arranged by size: 3074 2103 988 939 552 486 225 154 45 SwaI ATTT'AAAT Cuts at: 5220 5220 Size: 8566 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3951 4138 6593 6610 6763 6948 7393 Size: 1255 2371 187 2455 17 153 185 445 Cuts at: 7393 325 Size: 1498 Fragments arranged by size: 2455 2371 1498 1255 445 187 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3640 3957 6812 Size: 274 80 33 51 1443 1663 317 2855 Cuts at: 6812 8524 96 Size: 1712 138 Fragments arranged by size: 2855 1712 1663 1443 317 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 4501 4533 5707 5739 8526 8558 4501 Size: 32 1174 32 2787 32 4509 Fragments arranged by size: 4509 2787 1174 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 4132 1316 Size: 2816 5750 XbaI T'CTAG_A Cuts at: 5902 5902 Size: 8566 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2542 2542 Size: 8566 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 8566 XmaI C'CCGG_G Cuts at: 1870 5891 1870 Size: 4021 4545 XmnI GAAnn'nnTTC Cuts at: 3912 6695 3912 Size: 2783 5783 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 6374 276 Size: 53 83 186 936 4840 2468 Fragments arranged by size: 4840 2468 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaLI ApoI AseI AsiSI AvaI BaeI BamHI BanI BanII BbsI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI Eco57MI EcoRI EcoRV FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NsiI NspI PasI PciI PflMI PmeI PpiI PshAI PsiI PsrI PstI PvuI PvuII SalI SbfI ScaI SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI SwaI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: ApaI AscI AvrII BbeI BbvCI Bpu10I BsiWI BstZ17I EcoICRI EcoNI EcoO109I FseI FspAI KasI NaeI NarI NgoMIV NruI PacI PfoI PmlI PpuMI PspOMI PspXI RsrII SacI SacII SanDI SapI SfiI SfoI SrfI