Restriction map for
pFN21AB5691
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# NO CUT ENZYMES ( 121 enzymes) #
##################################
AatII Acc65I AccII AccIII AcyI
AflII AhdI Alw44I AocI Aor51HI
ApaI ApaLI AscI AseI AsnI
Asp718I AspEI AviII AvrII BamHI
BbeI BbrPI BbsI BbvCI BcgI
BclI BfrI BglI BlnI BpuAI
BsaBI BsaHI BsaI BseAI BseRI
BsiEI BsiWI BsmBI BspDI BspEI
BspLU11I BsrBI BssHII Bst1107I BstBI
BstUI BstZ17I Bsu36I CfoI ClaI
CpoI DraI DraIII DrdI DsaI
EagI Eam1105I EclXI Eco47III Eco52I
Eco81I EcoNI EcoRI EcoRV EcoT22I
FauI FbaI FseI FspI HaeII
HhaI Hin1I HinP1I HindIII HpaI
KasI KpnI KspI MamI MfeI
MluI MroI MunI MvnI NaeI
NarI NcoI NdeI NgoMIV NheI
NotI NruI NsiI NspI NspV
PaeR7I PmaCI PmeI PmlI PshAI
PshBI PvuI RsrII SacII SbfI
ScaI SfiI SfoI SfuI SgrAI
SmaI SmlI SnaBI SpeI SphI
Sse8387I StuI SwaI XhoI XmaCI
XmaI
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# ONE CUT ENZYMES ( 60 enzymes) #
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AccI 569 GTCGAC GT^[AC][GT]AC
AclI 370 AACGTT AA^CGTT
AcsI 106 GAATTT [GA]^AATT[CT]
AflIII 412 ACGTGT A^C[GA][CT]GT
AgeI 2519 ACCGGT A^CCGGT
ApoI 106 GAATTT [GA]^AATT[CT]
Asp700I 1216 GAACAATTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspHI 1575 GAGCTC G[AT]GC[AT]^C
AspI 1978 GACCCAGTC GAC[ACGT]^[ACGT][ACGT]GTC
BalI 2220 TGGCCA TGG^CCA
BanI 275 GGCACC G^G[CT][GA]CC
BcnI 74 CCCGG CC^[GC]GG
BsaAI 411 TACGTG [CT]AC^GT[GA]
BsaWI 2519 ACCGGT [AT]^CCGG[AT]
BsgI 528 CTGCAC GTGCAG(16/14)
BsiHKAI 1575 GAGCTC G[AT]GC[AT]^C
BsmI 665 GAATGC GAATGC(1/-1)
Bsp1407I 313 TGTACA T^GTACA
BspHI 1920 TCATGA T^CATGA
BspMI 697 GCAGGT ACCTGC(4/8)
BsrFI 2519 ACCGGT [GA]^CCGG[CT]
BsrGI 313 TGTACA T^GTACA
BssSI 860 CACGAG CACGAG(-5/-1)
BstAPI 2256 GCATTTGATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstEII 2363 GGTCACC G^GT[ACGT]ACC
BstPI 2363 GGTCACC G^GT[ACGT]ACC
BstXI 1721 CCAAGCCATTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Cac8I 2297 GCCTGC GC[ACGT]^[ACGT]GC
Cfr10I 2519 ACCGGT [GA]^CCGG[CT]
EaeI 2220 TGGCCA [CT]^GGCC[GA]
EcoO65I 2363 GGTCACC G^GT[ACGT]ACC
EcoT14I 1814 CCAAGG C^C[AT][AT]GG
HgaI 2027 GACGC GACGC(5/10)
HincII 569 GTCGAC GT[CT]^[GA]AC
HindII 569 GTCGAC GT[CT]^[GA]AC
MluNI 2220 TGGCCA TGG^CCA
MscI 2220 TGGCCA TGG^CCA
MslI 770 CATCTCTGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MspA1I 1211 CAGCTG C[AC]G^C[GT]G
MwoI 2256 GCATTTGATGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
NciI 74 CCCGG CC^[GC]GG
PacI 559 TTAATTAA TTAAT^TAA
PflFI 1978 GACCCAGTC GAC[ACGT]^[ACGT][ACGT]GTC
PflMI 2177 CCAATTTCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PinAI 2519 ACCGGT A^CCGGT
Psp1406I 370 AACGTT AA^CGTT
PvuII 1211 CAGCTG CAG^CTG
RcaI 1920 TCATGA T^CATGA
SacI 1575 GAGCTC GAGCT^C
SalI 569 GTCGAC G^TCGAC
SapI 1948 GCTCTTC GCTCTTC(1/4)
SexAI 2016 ACCTGGT A^CC[AT]GGT
SspBI 313 TGTACA T^GTACA
SspI 357 AATATT AAT^ATT
StyI 1814 CCAAGG C^C[AT][AT]GG
Tth111I 1978 GACCCAGTC GAC[ACGT]^[ACGT][ACGT]GTC
Van91I 2177 CCAATTTCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XbaI 1516 TCTAGA T^CTAGA
XcmI 22 CCACACCTGTTGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmnI 1216 GAACAATTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 14 enzymes) #
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AlwI 1989 GATCC GGATC(4/5)
2238 GGATC
AlwNI 950 CAGATACTG CAG[ACGT][ACGT][ACGT]^CTG
2012 CAGGACCTG
AvaI 5 CTCGGG C^[CT]CG[GA]G
55 CTCGGG
BanII 1398 GAGCCC G[GA]GC[CT]^C
1575 GAGCTC
BciVI 2097 GGATAC GTATCC(6/5)
334 GTATCC
BglII 1102 AGATCT A^GATCT
2357 AGATCT
BlpI 1395 GCTGAGC GC^T[ACGT]AGC
2251 GCTGAGC
Bpu1102I 1395 GCTGAGC GC^T[ACGT]AGC
2251 GCTGAGC
BsoBI 5 CTCGGG C^[CT]CG[GA]G
55 CTCGGG
BsrDI 1534 CATTGC GCAATG(2/0)
1192 GCAATG
BstYI 1102 AGATCT [GA]^GATC[CT]
2357 AGATCT
CelII 1395 GCTGAGC GC^T[ACGT]AGC
2251 GCTGAGC
MflI 1102 AGATCT [GA]^GATC[CT]
2357 AGATCT
XhoII 1102 AGATCT [GA]^GATC[CT]
2357 AGATCT
#########################################
# 3 OR MORE CUT ENZYMES ( 63 enzymes) #
#########################################
AciI AfaI AluI AvaII BbvI
BfaI BmyI BpmI BsaJI BsiYI
BslI BsmAI BsmFI Bsp1286I BsrI
BssKI BstF5I BstNI Cfr13I DdeI
DpnII DraII EarI EcoO109I EcoRII
Fnu4HI FokI HaeIII HapII HinfI
HpaII HphI ItaI Ksp632I MaeI
MaeII MaeIII MboI MboII MnlI
MseI MspI MvaI NdeII NlaIII
NlaIV PleI PpuMI PstI RsaI
Sau3AI Sau96I ScrFI SfaNI SfcI
TaqI TfiI Tru9I TseI Tsp45I
Tsp509I TspRI TthHB8I