Restriction map for
pFN21AB5691
(Circular) (Six-base) MAPSORT of: pFN21AB5691.seq Check: 1260 from: 1 to: 7285
pFN21AB5691 7285 bp
With 182 enzymes: SgfI *
February 27, 2009 14:34 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 5095 278
Size: 53 83 186 936 3559 2468
Fragments arranged by size:
3559 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2575 4628 1937
Size: 638 2053 4594
Fragments arranged by size:
4594 2053 638
Acc65I G'GTAC_C
Cuts at: 4606 4606
Size: 7285
AclI AA'CG_TT
Cuts at: 1563 2376 5412 5785 1563
Size: 813 3036 373 3063
Fragments arranged by size:
3063 3036 813 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2808 3215 3645 3819 3903 3941 4048 5348
Size: 1176 407 430 174 84 38 107 1300
Cuts at: 5348 6732 7000 1632
Size: 1384 268 1917
Fragments arranged by size:
1917 1384 1300 1176 430 407 268 174
107 84 38
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 7088
AflIII A'CryG_T
Cuts at: 1203 2417 6184 7161 1203
Size: 1214 3767 977 1327
Fragments arranged by size:
3767 1327 1214 977
AgeI A'CCGG_T
Cuts at: 4524 6116 4524
Size: 1592 5693
AhdI GACnn_n'nnGTC
Cuts at: 6014 6014
Size: 7285
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 7285
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 7285
AlwNI CAG_nnn'CTG
Cuts at: 2960 4022 5028 6600 2960
Size: 1062 1006 1572 3645
Fragments arranged by size:
3645 1572 1062 1006
ApaLI G'TGCA_C
Cuts at: 5342 6498 5342
Size: 1156 6129
ApoI r'AATT_y
Cuts at: 1493 2111 4594 4820 4972 7081 1493
Size: 618 2483 226 152 2109 1697
Fragments arranged by size:
2483 2109 1697 618 226 152
AseI AT'TA_AT
Cuts at: 160 1032 5839 160
Size: 872 4807 1606
Fragments arranged by size:
4807 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7285
AvaI C'yCGr_G
Cuts at: 1870 1943 2010 2060 4610 1870
Size: 73 67 50 2550 4545
Fragments arranged by size:
4545 2550 73 67 50
BamHI G'GATC_C
Cuts at: 4615 4982 4615
Size: 367 6918
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2280 4606 6061 618
Size: 325 217 627 493 2326 1455 1842
Fragments arranged by size:
2326 1842 1455 627 493 325 217
BanII G_rGCy'C
Cuts at: 1391 3407 3584 4604 1391
Size: 2016 177 1020 4072
Fragments arranged by size:
4072 2016 1020 177
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 6314
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 5476 5510 1903
Size: 34 3539 34 3678
Fragments arranged by size:
3678 3539 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2350 4096 5177 6387 2350
Size: 1746 1081 1210 3248
Fragments arranged by size:
3248 1746 1210 1081
BclI T'GATC_A
Cuts at: 1548 1548
Size: 7285
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 5896 136
Size: 107 122 71 631 605 4224 1525
Fragments arranged by size:
4224 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 3107 4362 7280 3107
Size: 1255 2918 3112
Fragments arranged by size:
3112 2918 1255
BlpI GC'TnA_GC
Cuts at: 3401 4257 4702 3401
Size: 856 445 5984
Fragments arranged by size:
5984 856 445
Bme1580I G_kGCm'C
Cuts at: 1790 2283 2681 5346 6502 1790
Size: 493 398 2665 1156 2573
Fragments arranged by size:
2665 2573 1156 493 398
BmgBI CAC'GTC
Cuts at: 1318 2044 2579 2589 1318
Size: 726 535 10 6014
Fragments arranged by size:
6014 726 535 10
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3674 3921 3980
Size: 416 53 83 399 24 2257 247 59
Cuts at: 3980 5016 5974 442
Size: 1036 958 1753
Fragments arranged by size:
2257 1753 1036 958 416 399 247 83
59 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 7285
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2087 2211 2370 3198 3306 4144 5945 1239
Size: 848 124 159 828 108 838 1801 2579
Fragments arranged by size:
2579 1801 848 838 828 159 124 108
Bpu10I CC'TnA_GC
Cuts at: 3261 3885 3261
Size: 624 6661
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 5410 6275 6573 5410
Size: 865 298 6122
Fragments arranged by size:
6122 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 5948 882
Size: 617 4449 2219
Fragments arranged by size:
4449 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 2418 5076 493
Size: 711 1214 2658 2702
Fragments arranged by size:
2702 2658 1214 711
BsaBI GATnn'nnATC
Cuts at: 7279 7279
Size: 7285
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 5092 5474 6109 275
Size: 53 83 186 936 3559 382 635 1451
Fragments arranged by size:
3559 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4524 5718 6116 6390 6537 6948 4524
Size: 1194 398 274 147 411 4861
Fragments arranged by size:
4861 1194 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2060 2090 2343 2373 3265
Size: 30 165 30 657 30 253 30 892
Cuts at: 3265 3295 3604 3634 1178
Size: 30 309 30 4829
Fragments arranged by size:
4829 892 657 309 253 165 30 30
30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 7285
BseYI C'CCAG_C
Cuts at: 2674 6488 6984 2674
Size: 3814 496 2975
Fragments arranged by size:
3814 2975 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2518 1163
Size: 573 782 5930
Fragments arranged by size:
5930 782 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 4735 5496 6524 1540
Size: 275 186 2734 761 1028 2301
Fragments arranged by size:
2734 2301 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 3584 5346 5431 6502 3584
Size: 1762 85 1071 4367
Fragments arranged by size:
4367 1762 1071 85
BsmI GAATG_Cn'
Cuts at: 2676 4796 4889 2676
Size: 2120 93 5072
Fragments arranged by size:
5072 2120 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2283 2681 3407 3584 4604 5346 5431
Size: 399 493 398 726 177 1020 742 85
Cuts at: 5431 6502 1391
Size: 1071 2174
Fragments arranged by size:
2174 1071 1020 742 726 493 399 398
177 85
BspEI T'CCGG_A
Cuts at: 6948 6948
Size: 7285
BspHI T'CATG_A
Cuts at: 3925 5174 6904 3925
Size: 1249 1730 4306
Fragments arranged by size:
4306 1730 1249
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2693 4640 844
Size: 1849 1947 3489
Fragments arranged by size:
3489 1947 1849
BsrBI CCG'CTC
Cuts at: 1394 1823 5172 1394
Size: 429 3349 3507
Fragments arranged by size:
3507 3349 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 3204 3538 5780 5954 65
Size: 1183 1956 334 2242 174 1396
Fragments arranged by size:
2242 1956 1396 1183 334 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4524 5929 6116 1700
Size: 110 2714 1405 187 2869
Fragments arranged by size:
2869 2714 1405 187 110
BsrGI T'GTAC_A
Cuts at: 96 2318 96
Size: 2222 5063
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 7285
BssSI C'ACGA_G
Cuts at: 2865 5345 6357 2865
Size: 2480 1012 3793
Fragments arranged by size:
3793 2480 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 4267 794
Size: 3473 3812
BstBI TT'CG_AA
Cuts at: 6089 6089
Size: 7285
BstEII G'GTnAC_C
Cuts at: 1184 4368 1184
Size: 3184 4101
BstXI CCAn_nnnn'nTGG
Cuts at: 3733 3733
Size: 7285
BstYI r'GATC_y
Cuts at: 1972 3107 4362 4615 4982 5367 5384 6836 6922
Size: 1135 1255 253 367 385 17 1452 86
Cuts at: 6922 6934 7280 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 1255 1135 385 367 346 253
86 17 12
BtgI C'CryG_G
Cuts at: 513 1065 513
Size: 552 6733
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 4136 487
Size: 683 56 2910 3636
Fragments arranged by size:
3636 2910 683 56
BtsI GCAGTG_nn'
Cuts at: 4251 4801 5595 5615 4251
Size: 550 794 20 5921
Fragments arranged by size:
5921 794 550 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 7285
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 2016 2051 2575 2610 529
Size: 35 1452 35 524 35 5204
Fragments arranged by size:
5204 1452 524 35 35 35
DraI TTT'AAA
Cuts at: 4589 4589
Size: 7285
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 6292 809
Size: 507 4976 1802
Fragments arranged by size:
4976 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 4225 4732 5621
Size: 54 1425 50 275 18 2395 507 889
Cuts at: 5621 7250 8
Size: 1629 43
Fragments arranged by size:
2395 1629 1425 889 507 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 4732 1537
Size: 275 2920 4090
Fragments arranged by size:
4090 2920 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2101 2827 2886 2893 2898 3156 3960 4374
Size: 748 726 59 7 5 258 804 414
Cuts at: 4374 5215 1353
Size: 841 3423
Fragments arranged by size:
3423 841 804 748 726 414 258 59
7 5
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 5872 6246 6392 387
Size: 1064 138 4283 374 146 1280
Fragments arranged by size:
4283 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 3582 3582
Size: 7285
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2087 2211 2370 2808 3198 3215 3306
Size: 393 455 124 159 438 390 17 91
Cuts at: 3306 3645 3819 3903 3941 4048 4144 5348 5945
Size: 339 174 84 38 107 96 1204 597
Cuts at: 5945 6732 7000 1239
Size: 787 268 1524
Fragments arranged by size:
1524 1204 787 597 455 438 393 390
339 268 174 159 124 107 96 91
84 38 17
EcoO109I rG'GnC_Cy
Cuts at: 2055 2071 4019 2055
Size: 16 1948 5321
Fragments arranged by size:
5321 1948 16
EcoRI G'AATT_C
Cuts at: 4594 4972 4594
Size: 378 6907
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2159 2159
Size: 7285
FspI TGC'GCA
Cuts at: 5791 5791
Size: 7285
HaeII r_GCGC'y
Cuts at: 1678 6432 1678
Size: 4754 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2060 2092 2100 2132 3035
Size: 32 135 32 488 32 8 32 903
Cuts at: 3035 3067 3265 3297 3637 3669 3728 3760 4019
Size: 32 198 32 340 32 59 32 259
Cuts at: 4019 4051 5926 5958 6000 6032 1373
Size: 32 1875 32 42 32 2626
Fragments arranged by size:
2626 1875 903 488 340 259 198 135
59 42 32 32 32 32 32 32
32 32 32 32 32 8
HincII GTy'rAC
Cuts at: 669 1657 1938 2576 4629 4875 669
Size: 988 281 638 2053 246 3079
Fragments arranged by size:
3079 2053 988 638 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 7285
HpaI GTT'AAC
Cuts at: 4875 4875
Size: 7285
KpnI G_GTAC'C
Cuts at: 4610 4610
Size: 7285
MfeI C'AATT_G
Cuts at: 4884 4884
Size: 7285
MluI A'CGCG_T
Cuts at: 7161 7161
Size: 7285
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2159 3765 4509 6399 6583 1305
Size: 854 1606 744 1890 184 2007
Fragments arranged by size:
2007 1890 1606 854 744 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 4227 10
Size: 54 1425 343 2395 3068
Fragments arranged by size:
3068 2395 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2779 5243 5602 5761 518
Size: 648 1613 2464 359 159 2042
Fragments arranged by size:
2464 2042 1613 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 3218 4701 5378 6526 6771 1606
Size: 1612 1483 677 1148 245 2120
Fragments arranged by size:
2120 1612 1483 1148 677 245
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 6733
NdeI CA'TA_TG
Cuts at: 387 387
Size: 7285
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 7285
NotI GC'GGCC_GC
Cuts at: 4732 4732
Size: 7285
NspI r_CATG'y
Cuts at: 1132 1229 4643 6188 1132
Size: 97 3414 1545 2229
Fragments arranged by size:
3414 2229 1545 97
PacI TTA_AT'TAA
Cuts at: 2568 2568
Size: 7285
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 7285
PciI A'CATG_T
Cuts at: 6184 6184
Size: 7285
PflMI CCAn_nnn'nTGG
Cuts at: 1108 4188 1108
Size: 3080 4205
PfoI T'CCnGG_A
Cuts at: 4160 4160
Size: 7285
PmeI GTTT'AAAC
Cuts at: 4589 4589
Size: 7285
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3878 3910 5354 5386 6867 6899 3878
Size: 32 1444 32 1481 32 4264
Fragments arranged by size:
4264 1481 1444 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 2055 2071 4019 2055
Size: 16 1948 5321
Fragments arranged by size:
5321 1948 16
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 7285
PsiI TTA'TAA
Cuts at: 4143 4421 4855 4143
Size: 278 434 6573
Fragments arranged by size:
6573 434 278
PstI C_TGCA'G
Cuts at: 830 2768 3673 4308 4637 830
Size: 1938 905 635 329 3478
Fragments arranged by size:
3478 1938 905 635 329
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 7285
PvuII CAG'CTG
Cuts at: 3218 3218
Size: 7285
SacI G_AGCT'C
Cuts at: 3584 3584
Size: 7285
SalI G'TCGA_C
Cuts at: 1936 2574 4627 1936
Size: 638 2053 4594
Fragments arranged by size:
4594 2053 638
SapI GCTCTTCn'nnn_
Cuts at: 3960 3960
Size: 7285
SbfI CC_TGCA'GG
Cuts at: 4637 4637
Size: 7285
ScaI AGT'ACT
Cuts at: 5533 7245 5533
Size: 1712 5573
SexAI A'CCwGG_T
Cuts at: 4021 4021
Size: 7285
SfcI C'TryA_G
Cuts at: 826 1045 2688 2764 3445 3669 4304 4633 5768
Size: 219 1643 76 681 224 635 329 1135
Cuts at: 5768 6449 6640 826
Size: 681 191 1471
Fragments arranged by size:
1643 1471 1135 681 681 635 329 224
219 191 76
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7285
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 7285
SmaI CCC'GGG
Cuts at: 1872 4612 1872
Size: 2740 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 5389 6290 6552 820
Size: 197 926 3446 901 262 1553
Fragments arranged by size:
3446 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 5076 493
Size: 4583 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 7285
SphI G_CATG'C
Cuts at: 1132 4643 1132
Size: 3511 3774
SspI AAT'ATT
Cuts at: 5 52 2364 5209 5
Size: 47 2312 2845 2081
Fragments arranged by size:
2845 2312 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 7285
StyI C'CwwG_G
Cuts at: 513 1065 3819 4724 513
Size: 552 2754 905 3074
Fragments arranged by size:
3074 2754 905 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 5312 5329 5482 5667 6112 325
Size: 1255 3732 17 153 185 445 1498
Fragments arranged by size:
3732 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2318 5531 7243
Size: 274 80 33 51 1443 341 3213 1712
Cuts at: 7243 96
Size: 138
Fragments arranged by size:
3213 1712 1443 341 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 7245 7277 7245
Size: 32 7253
Tth111I GACn'n_nGTC
Cuts at: 1316 3986 1316
Size: 2670 4615
XbaI T'CTAG_A
Cuts at: 3521 4621 3521
Size: 1100 6185
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2034 2034
Size: 7285
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 7285
XmaI C'CCGG_G
Cuts at: 1870 4610 1870
Size: 2740 4545
XmnI GAAnn'nnTTC
Cuts at: 3225 5414 3225
Size: 2189 5096
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 5093 276
Size: 53 83 186 936 3559 2468
Fragments arranged by size:
3559 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbsI BcgI BciVI BclI
BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI
Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI
BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI
ClaI CspCI DraI DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I
HincII HindIII HpaI KpnI MfeI MluI MmeI MscI
MslI MspA1I NcoI NdeI NheI NotI NspI PacI
PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI
PsiI PstI PvuI PvuII SacI SalI SapI SbfI
ScaI SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI
SpeI SphI SspI StuI StyI TaqII TatI TstI
Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbeI
BbvCI BfrBI BplI BsiWI BsmBI BstZ17I Bsu36I DraIII
EcoNI EcoRV FseI FspAI KasI NaeI NarI NgoMIV
NruI NsiI PmlI PspOMI PspXI PsrI RsrII SacII
SanDI SfiI SfoI SrfI SwaI