Restriction map for
pFN21AB5691
(Linear) (Six-base) MAPSORT of: KIBB5691.seq Check: 3481 from: 1 to: 2580
KIBB5691.orf
With 182 enzymes: SgfI *
September 18, 2009 17:00 ..
AccI GT'mk_AC
Cuts at: 0 570 2580
Size: 570 2010
AclI AA'CG_TT
Cuts at: 0 371 2580
Size: 371 2209
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 803 1210 1640 1814 1898 1936 2043 2580
Size: 803 407 430 174 84 38 107 537
Fragments arranged by size:
803 537 430 407 174 107 84 38
AflIII A'CryG_T
Cuts at: 0 412 2580
Size: 412 2168
AgeI A'CCGG_T
Cuts at: 0 2519 2580
Size: 2519 61
AlwNI CAG_nnn'CTG
Cuts at: 0 955 2017 2580
Size: 955 1062 563
Fragments arranged by size:
1062 955 563
ApoI r'AATT_y
Cuts at: 0 106 2580
Size: 106 2474
AvaI C'yCGr_G
Cuts at: 0 5 55 2580
Size: 5 50 2525
Fragments arranged by size:
2525 50 5
BanI G'GyrC_C
Cuts at: 0 275 2580
Size: 275 2305
BanII G_rGCy'C
Cuts at: 0 1402 1579 2580
Size: 1402 177 1001
Fragments arranged by size:
1402 1001 177
BciVI GTATCCnnnnn_n'
Cuts at: 0 345 2091 2580
Size: 345 1746 489
Fragments arranged by size:
1746 489 345
BglII A'GATC_T
Cuts at: 0 1102 2357 2580
Size: 1102 1255 223
Fragments arranged by size:
1255 1102 223
BlpI GC'TnA_GC
Cuts at: 0 1396 2252 2580
Size: 1396 856 328
Fragments arranged by size:
1396 856 328
Bme1580I G_kGCm'C
Cuts at: 0 278 676 2580
Size: 278 398 1904
Fragments arranged by size:
1904 398 278
BmgBI CAC'GTC
Cuts at: 0 39 574 584 2580
Size: 39 535 10 1996
Fragments arranged by size:
1996 535 39 10
BmrI ACTGGGnnnn_n'
Cuts at: 0 1669 1916 1975 2580
Size: 1669 247 59 605
Fragments arranged by size:
1669 605 247 59
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 82 206 365 1193 1301 2139 2580
Size: 82 124 159 828 108 838 441
Fragments arranged by size:
838 828 441 159 124 108 82
Bpu10I CC'TnA_GC
Cuts at: 0 1256 1880 2580
Size: 1256 624 700
Fragments arranged by size:
1256 700 624
BsaAI yAC'GTr
Cuts at: 0 413 2580
Size: 413 2167
BsaWI w'CCGG_w
Cuts at: 0 2519 2580
Size: 2519 61
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 55 85 338 368 1260 1290 1599 1629
Size: 55 30 253 30 892 30 309 30
Cuts at: 1629 2580
Size: 951
Fragments arranged by size:
951 892 309 253 55 30 30 30
30
BseYI C'CCAG_C
Cuts at: 0 669 2580
Size: 669 1911
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 513 2580
Size: 513 2067
BsiHKAI G_wGCw'C
Cuts at: 0 1579 2580
Size: 1579 1001
BsmI GAATG_Cn'
Cuts at: 0 671 2580
Size: 671 1909
Bsp1286I G_dGCh'C
Cuts at: 0 278 676 1402 1579 2580
Size: 278 398 726 177 1001
Fragments arranged by size:
1001 726 398 278 177
BspHI T'CATG_A
Cuts at: 0 1920 2580
Size: 1920 660
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 688 2580
Size: 688 1892
BsrDI GCAATG_nn'
Cuts at: 0 1199 1533 2580
Size: 1199 334 1047
Fragments arranged by size:
1199 1047 334
BsrFI r'CCGG_y
Cuts at: 0 2519 2580
Size: 2519 61
BsrGI T'GTAC_A
Cuts at: 0 313 2580
Size: 313 2267
BssSI C'ACGA_G
Cuts at: 0 860 2580
Size: 860 1720
BstAPI GCAn_nnn'nTGC
Cuts at: 0 2262 2580
Size: 2262 318
BstEII G'GTnAC_C
Cuts at: 0 2363 2580
Size: 2363 217
BstXI CCAn_nnnn'nTGG
Cuts at: 0 1728 2580
Size: 1728 852
BstYI r'GATC_y
Cuts at: 0 1102 2357 2580
Size: 1102 1255 223
Fragments arranged by size:
1255 1102 223
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 2131 2580
Size: 2131 449
BtsI GCAGTG_nn'
Cuts at: 0 2246 2580
Size: 2246 334
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 11 46 570 605 2580
Size: 11 35 524 35 1975
Fragments arranged by size:
1975 524 35 35 11
EaeI y'GGCC_r
Cuts at: 0 2220 2580
Size: 2220 360
EarI CTCTTCn'nnn_
Cuts at: 0 96 822 881 888 893 1151 1955 2369
Size: 96 726 59 7 5 258 804 414
Cuts at: 2369 2580
Size: 211
Fragments arranged by size:
804 726 414 258 211 96 59 7
5
EcoICRI GAG'CTC
Cuts at: 0 1577 2580
Size: 1577 1003
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 82 206 365 803 1193 1210 1301 1640
Size: 82 124 159 438 390 17 91 339
Cuts at: 1640 1814 1898 1936 2043 2139 2580
Size: 174 84 38 107 96 441
Fragments arranged by size:
441 438 390 339 174 159 124 107
96 91 84 82 38 17
EcoO109I rG'GnC_Cy
Cuts at: 0 50 66 2014 2580
Size: 50 16 1948 566
Fragments arranged by size:
1948 566 50 16
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 154 2580
Size: 154 2426
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 55 87 95 127 1030 1062 1260 1292
Size: 55 32 8 32 903 32 198 32
Cuts at: 1292 1632 1664 1723 1755 2014 2046 2580
Size: 340 32 59 32 259 32 534
Fragments arranged by size:
903 534 340 259 198 59 55 32
32 32 32 32 32 32 8
HincII GTy'rAC
Cuts at: 0 571 2580
Size: 571 2009
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 154 1760 2504 2580
Size: 154 1606 744 76
Fragments arranged by size:
1606 744 154 76
MscI TGG'CCA
Cuts at: 0 2222 2580
Size: 2222 358
MslI CAynn'nnrTG
Cuts at: 0 774 2580
Size: 774 1806
MspA1I CmG'CkG
Cuts at: 0 1213 2580
Size: 1213 1367
PacI TTA_AT'TAA
Cuts at: 0 563 2580
Size: 563 2017
PflMI CCAn_nnn'nTGG
Cuts at: 0 2183 2580
Size: 2183 397
PfoI T'CCnGG_A
Cuts at: 0 2155 2580
Size: 2155 425
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1873 1905 2580
Size: 1873 32 675
Fragments arranged by size:
1873 675 32
PpuMI rG'GwC_Cy
Cuts at: 0 50 66 2014 2580
Size: 50 16 1948 566
Fragments arranged by size:
1948 566 50 16
PsiI TTA'TAA
Cuts at: 0 2138 2416 2580
Size: 2138 278 164
Fragments arranged by size:
2138 278 164
PstI C_TGCA'G
Cuts at: 0 763 1668 2303 2580
Size: 763 905 635 277
Fragments arranged by size:
905 763 635 277
PvuII CAG'CTG
Cuts at: 0 1213 2580
Size: 1213 1367
SacI G_AGCT'C
Cuts at: 0 1579 2580
Size: 1579 1001
SalI G'TCGA_C
Cuts at: 0 569 2580
Size: 569 2011
SapI GCTCTTCn'nnn_
Cuts at: 0 1955 2580
Size: 1955 625
SexAI A'CCwGG_T
Cuts at: 0 2016 2580
Size: 2016 564
SfcI C'TryA_G
Cuts at: 0 683 759 1440 1664 2299 2580
Size: 683 76 681 224 635 281
Fragments arranged by size:
683 681 635 281 224 76
SspI AAT'ATT
Cuts at: 0 359 2580
Size: 359 2221
StyI C'CwwG_G
Cuts at: 0 1814 2580
Size: 1814 766
TatI w'GTAC_w
Cuts at: 0 313 2580
Size: 313 2267
Tth111I GACn'n_nGTC
Cuts at: 0 1981 2580
Size: 1981 599
XbaI T'CTAG_A
Cuts at: 0 1516 2580
Size: 1516 1064
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 29 2580
Size: 29 2551
XmnI GAAnn'nnTTC
Cuts at: 0 1220 2580
Size: 1220 1360
Enzymes that do cut:
AccI AclI AcuI AflIII AgeI AlwNI ApoI AvaI
BanI BanII BciVI BglII BlpI Bme1580I BmgBI BmrI
BpmI Bpu10I BsaAI BsaWI BsaXI BseYI BsgI BsiHKAI
BsmI Bsp1286I BspHI BspMI BsrDI BsrFI BsrGI BssSI
BstAPI BstEII BstXI BstYI BtgZI BtsI CspCI EaeI
EarI EcoICRI Eco57MI EcoO109I FalI Hin4I HincII MmeI
MscI MslI MspA1I PacI PflMI PfoI PpiI PpuMI
PsiI PstI PvuII SacI SalI SapI SexAI SfcI
SspI StyI TatI Tth111I XbaI XcmI XmnI
Enzymes that do not cut:
AarI AatII Acc65I AfeI AflII AhdI AleI AlfI
AloI ApaI ApaLI AscI AseI AsiSI AvrII BaeI
BamHI BbeI BbsI BbvCI BcgI BclI BfrBI BglI
BmtI BplI BpuEI BsaI BsaBI BsaHI BseRI BsiEI
BsiWI BsmBI BspEI BsrBI BssHII BstBI BstZ17I Bsu36I
BtgI ClaI DraI DraIII DrdI EagI EciI EcoNI
EcoRI EcoRV FseI FspI FspAI HaeII HindIII HpaI
KasI KpnI MfeI MluI NaeI NarI NcoI NdeI
NgoMIV NheI NotI NruI NsiI NspI PasI PciI
PmeI PmlI PshAI PspOMI PspXI PsrI PvuI RsrII
SacII SanDI SbfI ScaI SfiI SfoI SgfI SgrAI
SmaI SmlI SnaBI SpeI SphI SrfI StuI SwaI
TaqII TstI XhoI XmaI ZraI