(Circular) (Six-base) MAPSORT of: pFN21AB5674.seq Check: 73 from: 1 to: 6010 pFN21AB5674 6010 bp With 182 enzymes: SgfI * January 16, 2009 15:25 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 2151 3820 278 Size: 53 83 186 936 615 1669 2468 Fragments arranged by size: 2468 1669 936 615 186 83 53 AccI GT'mk_AC Cuts at: 1937 3353 1937 Size: 1416 4594 Acc65I G'GTAC_C Cuts at: 3331 3331 Size: 6010 AclI AA'CG_TT Cuts at: 1563 4137 4510 1563 Size: 2574 373 3063 Fragments arranged by size: 3063 2574 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2463 3129 4073 5457 5725 1632 Size: 831 666 944 1384 268 1917 Fragments arranged by size: 1917 1384 944 831 666 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5813 AflIII A'CryG_T Cuts at: 1203 3240 4909 5886 1203 Size: 2037 1669 977 1327 Fragments arranged by size: 2037 1669 1327 977 AgeI A'CCGG_T Cuts at: 4841 4841 Size: 6010 AhdI GACnn_n'nnGTC Cuts at: 4739 4739 Size: 6010 AleI CACnn'nnGTG Cuts at: 1166 2649 1166 Size: 1483 4527 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2667 1184 Size: 1483 4527 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2006 2038 2006 Size: 32 5978 AlwNI CAG_nnn'CTG Cuts at: 3753 5325 3753 Size: 1572 4438 ApaI G_GGCC'C Cuts at: 2264 2634 2264 Size: 370 5640 ApaLI G'TGCA_C Cuts at: 2642 4067 5223 2642 Size: 1425 1156 3429 Fragments arranged by size: 3429 1425 1156 ApoI r'AATT_y Cuts at: 1493 2419 2840 3319 3545 3697 5806 1493 Size: 926 421 479 226 152 2109 1697 Fragments arranged by size: 2109 1697 926 479 421 226 152 AseI AT'TA_AT Cuts at: 160 1032 4564 160 Size: 872 3532 1606 Fragments arranged by size: 3532 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6010 AvaI C'yCGr_G Cuts at: 1870 1943 2508 2912 3188 3335 1870 Size: 73 565 404 276 147 4545 Fragments arranged by size: 4545 565 404 276 147 73 BamHI G'GATC_C Cuts at: 3340 3707 3340 Size: 367 5643 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2834 3146 3331 4786 618 Size: 325 217 627 1047 312 185 1455 1842 Fragments arranged by size: 1842 1455 1047 627 325 312 217 185 BanII G_rGCy'C Cuts at: 1391 2264 2634 2907 3329 1391 Size: 873 370 273 422 4072 Fragments arranged by size: 4072 873 422 370 273 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2418 3131 928 Size: 971 519 713 3807 Fragments arranged by size: 3807 971 713 519 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 2305 2339 3188 3222 4201 4235 1903 Size: 34 368 34 849 34 979 34 3678 Fragments arranged by size: 3678 979 849 368 34 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3902 5112 3902 Size: 1210 4800 BclI T'GATC_A Cuts at: 1548 1548 Size: 6010 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4621 136 Size: 107 122 71 631 605 2949 1525 Fragments arranged by size: 2949 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 2514 6005 2514 Size: 3491 2519 BlpI GC'TnA_GC Cuts at: 3427 3427 Size: 6010 Bme1580I G_kGCm'C Cuts at: 1790 2264 2634 2646 3151 4071 5227 1790 Size: 474 370 12 505 920 1156 2573 Fragments arranged by size: 2573 1156 920 505 474 370 12 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6010 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3741 4699 442 Size: 416 53 83 399 24 2324 958 1753 Fragments arranged by size: 2324 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6010 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2921 2975 4670 1239 Size: 1682 54 1695 2579 Fragments arranged by size: 2579 1695 1682 54 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4135 5000 5298 4135 Size: 865 298 4847 Fragments arranged by size: 4847 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 3008 4673 882 Size: 617 1509 1665 2219 Fragments arranged by size: 2219 1665 1509 617 BsaAI yAC'GTr Cuts at: 493 1204 3801 493 Size: 711 2597 2702 Fragments arranged by size: 2702 2597 711 BsaBI GATnn'nnATC Cuts at: 6004 6004 Size: 6010 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2148 3817 4199 4834 Size: 53 83 186 936 615 1669 382 635 Cuts at: 4834 275 Size: 1451 Fragments arranged by size: 1669 1451 936 635 615 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4443 4841 5115 5262 5673 4443 Size: 398 274 147 411 4780 Fragments arranged by size: 4780 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2285 2315 1178 Size: 30 165 30 882 30 4873 Fragments arranged by size: 4873 882 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 1186 Size: 6010 BseYI C'CCAG_C Cuts at: 2049 3163 5213 5709 2049 Size: 1114 2050 496 2350 Fragments arranged by size: 2350 2050 1114 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2673 2885 1163 Size: 573 937 212 4288 Fragments arranged by size: 4288 937 573 212 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3267 3460 4221 5249 1540 Size: 275 186 1266 193 761 1028 2301 Fragments arranged by size: 2301 1266 1028 761 275 193 186 BsiHKAI G_wGCw'C Cuts at: 2628 2646 4071 4156 5227 2628 Size: 18 1425 85 1071 3411 Fragments arranged by size: 3411 1425 1071 85 18 BsmI GAATG_Cn' Cuts at: 3521 3614 3521 Size: 93 5917 BsmBI CGTCTCn'nnnn_ Cuts at: 2139 2139 Size: 6010 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2264 2628 2634 2646 2907 3151 3329 Size: 399 474 364 6 12 261 244 178 Cuts at: 3329 4071 4156 5227 1391 Size: 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 474 399 364 261 244 178 85 12 6 BspEI T'CCGG_A Cuts at: 5673 5673 Size: 6010 BspHI T'CATG_A Cuts at: 3899 5629 3899 Size: 1730 4280 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2655 2702 3365 844 Size: 1811 47 663 3489 Fragments arranged by size: 3489 1811 663 47 BsrBI CCG'CTC Cuts at: 1394 1823 3897 1394 Size: 429 2074 3507 Fragments arranged by size: 3507 2074 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4505 4679 65 Size: 1183 3257 174 1396 Fragments arranged by size: 3257 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 3266 4654 4841 1700 Size: 110 1456 1388 187 2869 Fragments arranged by size: 2869 1456 1388 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6010 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6010 BssSI C'ACGA_G Cuts at: 2891 4070 5082 2891 Size: 1179 1012 3819 Fragments arranged by size: 3819 1179 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6010 BstBI TT'CG_AA Cuts at: 4814 4814 Size: 6010 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6010 BstXI CCAn_nnnn'nTGG Cuts at: 2011 2011 Size: 6010 BstYI r'GATC_y Cuts at: 1972 2514 3340 3707 4092 4109 5561 5647 5659 Size: 542 826 367 385 17 1452 86 12 Cuts at: 5659 6005 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 826 542 385 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 513 Size: 552 939 4519 Fragments arranged by size: 4519 939 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5271 Fragments arranged by size: 5271 683 56 BtsI GCAGTG_nn' Cuts at: 2644 2656 3182 3526 4320 4340 2644 Size: 12 526 344 794 20 4314 Fragments arranged by size: 4314 794 526 344 20 12 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6010 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5975 DraI TTT'AAA Cuts at: 3314 3314 Size: 6010 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 2552 5017 809 Size: 507 1236 2465 1802 Fragments arranged by size: 2465 1802 1236 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2055 2106 2378 Size: 54 1425 50 275 18 225 51 272 Cuts at: 2378 2936 3264 3457 4346 5975 8 Size: 558 328 193 889 1629 43 Fragments arranged by size: 1629 1425 889 558 328 275 272 225 193 54 51 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3264 3457 1537 Size: 275 1452 193 4090 Fragments arranged by size: 4090 1452 275 193 EarI CTCTTCn'nnn_ Cuts at: 1353 2863 3006 3940 1353 Size: 1510 143 934 3423 Fragments arranged by size: 3423 1510 934 143 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4597 4971 5117 387 Size: 1064 138 3008 374 146 1280 Fragments arranged by size: 3008 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2463 2921 2975 3129 4073 4670 5457 Size: 393 831 458 54 154 944 597 787 Cuts at: 5457 5725 1239 Size: 268 1524 Fragments arranged by size: 1524 944 831 787 597 458 393 268 154 54 EcoO109I rG'GnC_Cy Cuts at: 2260 2261 2896 2260 Size: 1 635 5374 Fragments arranged by size: 5374 635 1 EcoRI G'AATT_C Cuts at: 3319 3697 3319 Size: 378 5632 EcoRV GAT'ATC Cuts at: 2071 2449 2740 2071 Size: 378 291 5341 Fragments arranged by size: 5341 378 291 FspI TGC'GCA Cuts at: 2368 4516 2368 Size: 2148 3862 FspAI rTGC'GCAy Cuts at: 2368 2368 Size: 6010 HaeII r_GCGC'y Cuts at: 1678 3273 5157 1678 Size: 1595 1884 2531 Fragments arranged by size: 2531 1884 1595 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2438 2470 2594 2626 4651 Size: 32 135 32 866 32 124 32 2025 Cuts at: 4651 4683 4725 4757 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 2025 866 135 124 42 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3238 3354 3600 669 Size: 988 281 1300 116 246 3079 Fragments arranged by size: 3079 1300 988 281 246 116 HindIII A'AGCT_T Cuts at: 748 2111 748 Size: 1363 4647 HpaI GTT'AAC Cuts at: 3600 3600 Size: 6010 KpnI G_GTAC'C Cuts at: 3335 3335 Size: 6010 MfeI C'AATT_G Cuts at: 3609 3609 Size: 6010 MluI A'CGCG_T Cuts at: 5886 5886 Size: 6010 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2098 2150 5124 5308 1305 Size: 793 52 2974 184 2007 Fragments arranged by size: 2974 2007 793 184 52 MscI TGG'CCA Cuts at: 10 64 1489 1832 2108 2380 10 Size: 54 1425 343 276 272 3640 Fragments arranged by size: 3640 1425 343 276 272 54 MslI CAynn'nnrTG Cuts at: 518 1166 2009 2649 3245 3968 4327 4486 518 Size: 648 843 640 596 723 359 159 2042 Fragments arranged by size: 2042 843 723 648 640 596 359 159 MspA1I CmG'CkG Cuts at: 1606 2803 3426 4103 5251 5496 1606 Size: 1197 623 677 1148 245 2120 Fragments arranged by size: 2120 1197 1148 677 623 245 NaeI GCC'GGC Cuts at: 3268 3268 Size: 6010 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 4519 Fragments arranged by size: 4519 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 6010 NgoMIV G'CCGG_C Cuts at: 3266 3266 Size: 6010 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6010 NotI GC'GGCC_GC Cuts at: 3457 3457 Size: 6010 NspI r_CATG'y Cuts at: 1132 1229 2280 3244 3368 4913 1132 Size: 97 1051 964 124 1545 2229 Fragments arranged by size: 2229 1545 1051 964 124 97 PasI CC'CwG_GG Cuts at: 1348 2257 1348 Size: 909 5101 PciI A'CATG_T Cuts at: 3240 4909 3240 Size: 1669 4341 PflMI CCAn_nnn'nTGG Cuts at: 1108 2966 1108 Size: 1858 4152 PfoI T'CCnGG_A Cuts at: 2042 2952 2042 Size: 910 5100 PmeI GTTT'AAAC Cuts at: 3314 3314 Size: 6010 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4079 4111 5592 5624 4079 Size: 32 1481 32 4465 Fragments arranged by size: 4465 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2896 2896 Size: 6010 PshAI GACnn'nnGTC Cuts at: 1613 2151 2166 1613 Size: 538 15 5457 Fragments arranged by size: 5457 538 15 PsiI TTA'TAA Cuts at: 3580 3580 Size: 6010 PspOMI G'GGCC_C Cuts at: 2260 2630 2260 Size: 370 5640 PstI C_TGCA'G Cuts at: 830 2651 3362 830 Size: 1821 711 3478 Fragments arranged by size: 3478 1821 711 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6010 PvuII CAG'CTG Cuts at: 2803 2803 Size: 6010 SalI G'TCGA_C Cuts at: 1936 3352 1936 Size: 1416 4594 SbfI CC_TGCA'GG Cuts at: 3362 3362 Size: 6010 ScaI AGT'ACT Cuts at: 4258 5970 4258 Size: 1712 4298 SfcI C'TryA_G Cuts at: 826 1045 2647 3283 3358 4493 5174 5365 826 Size: 219 1602 636 75 1135 681 191 1471 Fragments arranged by size: 1602 1471 1135 681 636 219 191 75 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6010 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6010 SmaI CCC'GGG Cuts at: 1872 3337 1872 Size: 1465 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2508 4114 5015 5277 820 Size: 197 926 565 1606 901 262 1553 Fragments arranged by size: 1606 1553 926 901 565 262 197 SnaBI TAC'GTA Cuts at: 493 3801 493 Size: 3308 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6010 SphI G_CATG'C Cuts at: 1132 3368 1132 Size: 2236 3774 SspI AAT'ATT Cuts at: 5 52 3934 5 Size: 47 3882 2081 Fragments arranged by size: 3882 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6010 StyI C'CwwG_G Cuts at: 513 1065 2004 3449 513 Size: 552 939 1445 3074 Fragments arranged by size: 3074 1445 939 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2956 2992 4037 4054 4207 4392 4837 Size: 1255 1376 36 1045 17 153 185 445 Cuts at: 4837 325 Size: 1498 Fragments arranged by size: 1498 1376 1255 1045 445 185 153 36 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 4256 5968 96 Size: 274 80 33 51 1443 2279 1712 138 Fragments arranged by size: 2279 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2285 2317 5970 6002 2285 Size: 32 3653 32 2293 Fragments arranged by size: 3653 2293 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6010 XbaI T'CTAG_A Cuts at: 3346 3346 Size: 6010 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2845 2950 2997 2845 Size: 105 47 5858 Fragments arranged by size: 5858 105 47 XhoI C'TCGA_G Cuts at: 1943 2508 1943 Size: 565 5445 XmaI C'CCGG_G Cuts at: 1870 3335 1870 Size: 1465 4545 XmnI GAAnn'nnTTC Cuts at: 3227 4139 3227 Size: 912 5098 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 2149 3818 276 Size: 53 83 186 936 615 1669 2468 Fragments arranged by size: 2468 1669 936 615 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI EcoRV FspI FspAI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AscI AvrII BaeI BbeI BbvCI BfrBI BplI Bpu10I BsiWI BstZ17I Bsu36I DraIII EcoICRI EcoNI FalI FseI KasI NarI NruI NsiI PacI PmlI PspXI PsrI RsrII SacI SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI