(Circular) (Six-base) MAPSORT of: pFN21AB5670.seq Check: 8116 from: 1 to: 7480 pFN21AB5670 7480 bp With 182 enzymes: SgfI * February 27, 2009 14:34 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2444 2444 Size: 7480 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 5290 278 Size: 53 83 186 936 3754 2468 Fragments arranged by size: 3754 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 4537 4823 1937 Size: 2600 286 4594 Fragments arranged by size: 4594 2600 286 Acc65I G'GTAC_C Cuts at: 4801 4801 Size: 7480 AclI AA'CG_TT Cuts at: 1563 2329 5607 5980 1563 Size: 766 3278 373 3063 Fragments arranged by size: 3278 3063 766 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2676 3183 3700 4292 5543 6927 7195 1632 Size: 1044 507 517 592 1251 1384 268 1917 Fragments arranged by size: 1917 1384 1251 1044 592 517 507 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 7283 AflIII A'CryG_T Cuts at: 1203 4266 6379 7356 1203 Size: 3063 2113 977 1327 Fragments arranged by size: 3063 2113 1327 977 AgeI A'CCGG_T Cuts at: 6311 6311 Size: 7480 AhdI GACnn_n'nnGTC Cuts at: 6209 6209 Size: 7480 AleI CACnn'nnGTG Cuts at: 1166 4039 1166 Size: 2873 4607 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 7480 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2195 2227 2195 Size: 32 7448 AlwNI CAG_nnn'CTG Cuts at: 4103 4714 5223 6795 4103 Size: 611 509 1572 4788 Fragments arranged by size: 4788 1572 611 509 ApaI G_GGCC'C Cuts at: 2153 2153 Size: 7480 ApaLI G'TGCA_C Cuts at: 5537 6693 5537 Size: 1156 6324 ApoI r'AATT_y Cuts at: 1493 2342 2418 2677 2855 3413 3498 3512 4367 Size: 849 76 259 178 558 85 14 855 Cuts at: 4367 4789 5015 5167 7276 1493 Size: 422 226 152 2109 1697 Fragments arranged by size: 2109 1697 855 849 558 422 259 226 178 152 85 76 14 AseI AT'TA_AT Cuts at: 160 1032 2400 6034 160 Size: 872 1368 3634 1606 Fragments arranged by size: 3634 1606 1368 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 7480 AvaI C'yCGr_G Cuts at: 1870 1943 4805 1870 Size: 73 2862 4545 Fragments arranged by size: 4545 2862 73 BamHI G'GATC_C Cuts at: 3811 4810 5177 3811 Size: 999 367 6114 Fragments arranged by size: 6114 999 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 4801 6256 618 Size: 325 217 627 3014 1455 1842 Fragments arranged by size: 3014 1842 1455 627 325 217 BanII G_rGCy'C Cuts at: 1391 2153 2196 4799 1391 Size: 762 43 2603 4072 Fragments arranged by size: 4072 2603 762 43 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2063 3846 928 Size: 971 164 1783 4562 Fragments arranged by size: 4562 1783 971 164 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3463 3497 4590 4624 5671 5705 1903 Size: 34 1526 34 1093 34 1047 34 3678 Fragments arranged by size: 3678 1526 1093 1047 34 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 5372 6582 5372 Size: 1210 6270 BclI T'GATC_A Cuts at: 1548 3159 1548 Size: 1611 5869 BfrBI ATG'CAT Cuts at: 3564 4055 3564 Size: 491 6989 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 6091 136 Size: 107 122 71 631 605 4419 1525 Fragments arranged by size: 4419 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 2599 3757 7475 2599 Size: 1158 3718 2604 Fragments arranged by size: 3718 2604 1158 BlpI GC'TnA_GC Cuts at: 3432 4897 3432 Size: 1465 6015 Bme1580I G_kGCm'C Cuts at: 1790 2153 4600 5541 6697 1790 Size: 363 2447 941 1156 2573 Fragments arranged by size: 2573 2447 1156 941 363 BmgBI CAC'GTC Cuts at: 1318 3995 1318 Size: 2677 4803 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2900 2996 4630 Size: 416 53 83 399 24 1483 96 1634 Cuts at: 4630 5211 6169 442 Size: 581 958 1753 Fragments arranged by size: 1753 1634 1483 958 581 416 399 96 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 7480 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2180 3000 3362 3371 4722 6140 1239 Size: 941 820 362 9 1351 1418 2579 Fragments arranged by size: 2579 1418 1351 941 820 362 9 Bpu10I CC'TnA_GC Cuts at: 2081 2081 Size: 7480 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2162 2624 5605 6470 6768 2162 Size: 462 2981 865 298 2874 Fragments arranged by size: 2981 2874 865 462 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 6143 882 Size: 617 4644 2219 Fragments arranged by size: 4644 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 5271 493 Size: 711 4067 2702 Fragments arranged by size: 4067 2702 711 BsaBI GATnn'nnATC Cuts at: 7474 7474 Size: 7480 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 5287 5669 6304 275 Size: 53 83 186 936 3754 382 635 1451 Fragments arranged by size: 3754 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2052 5913 6311 6585 6732 7143 2052 Size: 3861 398 274 147 411 2389 Fragments arranged by size: 3861 2389 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 7255 Fragments arranged by size: 7255 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2321 1186 Size: 1135 6345 BseYI C'CCAG_C Cuts at: 3815 6683 7179 3815 Size: 2868 496 4116 Fragments arranged by size: 4116 2868 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2138 2478 1163 Size: 573 402 340 6165 Fragments arranged by size: 6165 573 402 340 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 4930 5691 6719 1540 Size: 275 186 2929 761 1028 2301 Fragments arranged by size: 2929 2301 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2196 2797 3919 5541 5626 6697 2196 Size: 601 1122 1622 85 1071 2979 Fragments arranged by size: 2979 1622 1122 1071 601 85 BsmI GAATG_Cn' Cuts at: 2480 2663 3128 4055 4493 4529 4991 5084 2480 Size: 183 465 927 438 36 462 93 4876 Fragments arranged by size: 4876 927 465 462 438 183 93 36 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2153 2196 2797 3919 4600 4799 5541 Size: 399 363 43 601 1122 681 199 742 Cuts at: 5541 5626 6697 1391 Size: 85 1071 2174 Fragments arranged by size: 2174 1122 1071 742 681 601 399 363 199 85 43 BspEI T'CCGG_A Cuts at: 2052 7143 2052 Size: 5091 2389 BspHI T'CATG_A Cuts at: 4360 5369 7099 4360 Size: 1009 1730 4741 Fragments arranged by size: 4741 1730 1009 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2234 2444 4835 844 Size: 1390 210 2391 3489 Fragments arranged by size: 3489 2391 1390 210 BsrBI CCG'CTC Cuts at: 1394 1823 5367 1394 Size: 429 3544 3507 Fragments arranged by size: 3544 3507 429 BsrDI GCAATG_nn' Cuts at: 65 1248 3727 3826 4119 4168 5975 6149 65 Size: 1183 2479 99 293 49 1807 174 1396 Fragments arranged by size: 2479 1807 1396 1183 293 174 99 49 BsrFI r'CCGG_y Cuts at: 1700 1810 6124 6311 1700 Size: 110 4314 187 2869 Fragments arranged by size: 4314 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 2715 4269 96 Size: 2619 1554 3307 Fragments arranged by size: 3307 2619 1554 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 7480 BssSI C'ACGA_G Cuts at: 5540 6552 5540 Size: 1012 6468 BstAPI GCAn_nnn'nTGC Cuts at: 794 4440 794 Size: 3646 3834 BstBI TT'CG_AA Cuts at: 6284 6284 Size: 7480 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 7480 BstXI CCAn_nnnn'nTGG Cuts at: 3221 3221 Size: 7480 BstYI r'GATC_y Cuts at: 1972 2599 2650 2901 3194 3757 3811 4810 5177 Size: 627 51 251 293 563 54 999 367 Cuts at: 5177 5562 5579 7031 7117 7129 7475 1972 Size: 385 17 1452 86 12 346 1977 Fragments arranged by size: 1977 1452 999 627 563 385 367 346 293 251 86 54 51 17 12 BtgI C'CryG_G Cuts at: 513 1065 2215 513 Size: 552 1150 5778 Fragments arranged by size: 5778 1150 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 6741 Fragments arranged by size: 6741 683 56 BtsI GCAGTG_nn' Cuts at: 2825 3206 4996 5790 5810 2825 Size: 381 1790 794 20 4495 Fragments arranged by size: 4495 1790 794 381 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 7480 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 4048 4081 4083 4116 529 Size: 35 3484 33 2 33 3893 Fragments arranged by size: 3893 3484 35 33 33 2 DraI TTT'AAA Cuts at: 2325 2347 2728 3418 3933 4784 2325 Size: 22 381 690 515 851 5021 Fragments arranged by size: 5021 851 690 515 381 22 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 6487 809 Size: 507 5171 1802 Fragments arranged by size: 5171 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 4927 5816 7445 Size: 54 1425 50 275 18 3097 889 1629 Cuts at: 7445 8 Size: 43 Fragments arranged by size: 3097 1629 1425 889 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 4927 1537 Size: 275 3115 4090 Fragments arranged by size: 4090 3115 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2106 3588 3858 4192 5410 1353 Size: 753 1482 270 334 1218 3423 Fragments arranged by size: 3423 1482 1218 753 334 270 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 6067 6441 6587 387 Size: 1064 138 4478 374 146 1280 Fragments arranged by size: 4478 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2194 2194 Size: 7480 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2180 2676 3000 3183 3362 3371 3700 Size: 393 548 496 324 183 179 9 329 Cuts at: 3700 4292 4722 5543 6140 6927 7195 1239 Size: 592 430 821 597 787 268 1524 Fragments arranged by size: 1524 821 787 597 592 548 496 430 393 329 324 268 183 179 9 EcoNI CCTnn'n_nnAGG Cuts at: 2101 3071 2101 Size: 970 6510 EcoO109I rG'GnC_Cy Cuts at: 2149 2149 Size: 7480 EcoRI G'AATT_C Cuts at: 2677 4789 5167 2677 Size: 2112 378 4990 Fragments arranged by size: 4990 2112 378 EcoRV GAT'ATC Cuts at: 2371 2371 Size: 7480 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 4543 4543 Size: 7480 FspI TGC'GCA Cuts at: 5986 5986 Size: 7480 HaeII r_GCGC'y Cuts at: 1678 6627 1678 Size: 4949 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 6121 6153 6195 6227 1373 Size: 32 135 32 4549 32 42 32 2626 Fragments arranged by size: 4549 2626 135 42 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 4824 5070 669 Size: 988 281 2886 246 3079 Fragments arranged by size: 3079 2886 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 7480 HpaI GTT'AAC Cuts at: 5070 5070 Size: 7480 KpnI G_GTAC'C Cuts at: 4805 4805 Size: 7480 MfeI C'AATT_G Cuts at: 5079 5079 Size: 7480 MluI A'CGCG_T Cuts at: 7356 7356 Size: 7480 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2169 2283 6594 6778 1305 Size: 864 114 4311 184 2007 Fragments arranged by size: 4311 2007 864 184 114 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 5658 Fragments arranged by size: 5658 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 4019 4039 4172 5438 5797 5956 518 Size: 648 2853 20 133 1266 359 159 2042 Fragments arranged by size: 2853 2042 1266 648 359 159 133 20 MspA1I CmG'CkG Cuts at: 1606 2990 3436 4466 4896 5573 6721 6966 1606 Size: 1384 446 1030 430 677 1148 245 2120 Fragments arranged by size: 2120 1384 1148 1030 677 446 430 245 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 6928 NdeI CA'TA_TG Cuts at: 387 387 Size: 7480 NheI G'CTAG_C Cuts at: 1052 1052 Size: 7480 NotI GC'GGCC_GC Cuts at: 4927 4927 Size: 7480 NsiI A_TGCA'T Cuts at: 3566 4057 3566 Size: 491 6989 NspI r_CATG'y Cuts at: 1132 1229 3564 3677 4270 4458 4838 6383 1132 Size: 97 2335 113 593 188 380 1545 2229 Fragments arranged by size: 2335 2229 1545 593 380 188 113 97 PacI TTA_AT'TAA Cuts at: 2400 2400 Size: 7480 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 7480 PciI A'CATG_T Cuts at: 4266 6379 4266 Size: 2113 5367 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 7480 PfoI T'CCnGG_A Cuts at: 2040 2196 2040 Size: 156 7324 PmeI GTTT'AAAC Cuts at: 4784 4784 Size: 7480 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 5549 5581 7062 7094 5549 Size: 32 1481 32 5935 Fragments arranged by size: 5935 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 7480 PsiI TTA'TAA Cuts at: 3366 4664 5050 3366 Size: 1298 386 5796 Fragments arranged by size: 5796 1298 386 PspOMI G'GGCC_C Cuts at: 2149 2149 Size: 7480 PstI C_TGCA'G Cuts at: 830 3789 4436 4613 4832 830 Size: 2959 647 177 219 3478 Fragments arranged by size: 3478 2959 647 219 177 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 7480 PvuII CAG'CTG Cuts at: 2990 3436 4466 2990 Size: 446 1030 6004 Fragments arranged by size: 6004 1030 446 SacI G_AGCT'C Cuts at: 2196 2196 Size: 7480 SalI G'TCGA_C Cuts at: 1936 4822 1936 Size: 2886 4594 SapI GCTCTTCn'nnn_ Cuts at: 4192 4192 Size: 7480 SbfI CC_TGCA'GG Cuts at: 4832 4832 Size: 7480 ScaI AGT'ACT Cuts at: 5728 7440 5728 Size: 1712 5768 SfcI C'TryA_G Cuts at: 826 1045 2423 2799 3785 4432 4609 4828 5963 Size: 219 1378 376 986 647 177 219 1135 Cuts at: 5963 6644 6835 826 Size: 681 191 1471 Fragments arranged by size: 1471 1378 1135 986 681 647 376 219 219 191 177 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 7480 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 7480 SmaI CCC'GGG Cuts at: 1872 4807 1872 Size: 2935 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2177 2603 5584 6485 6747 820 Size: 197 926 234 426 2981 901 262 1553 Fragments arranged by size: 2981 1553 926 901 426 262 234 197 SnaBI TAC'GTA Cuts at: 493 5271 493 Size: 4778 2702 SpeI A'CTAG_T Cuts at: 152 3134 4229 152 Size: 2982 1095 3403 Fragments arranged by size: 3403 2982 1095 SphI G_CATG'C Cuts at: 1132 3564 3677 4838 1132 Size: 2432 113 1161 3774 Fragments arranged by size: 3774 2432 1161 113 SspI AAT'ATT Cuts at: 5 52 4081 5404 5 Size: 47 4029 1323 2081 Fragments arranged by size: 4029 2081 1323 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 7480 StyI C'CwwG_G Cuts at: 513 1065 3273 4449 4919 513 Size: 552 2208 1176 470 3074 Fragments arranged by size: 3074 2208 1176 552 470 SwaI ATTT'AAAT Cuts at: 3418 3418 Size: 7480 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 5507 5524 5677 5862 6307 325 Size: 1255 3927 17 153 185 445 1498 Fragments arranged by size: 3927 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2715 4269 4638 Size: 274 80 33 51 1443 738 1554 369 Cuts at: 4638 4716 5726 7438 96 Size: 78 1010 1712 138 Fragments arranged by size: 1712 1554 1443 1010 738 369 274 138 80 78 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 4617 4649 7440 7472 4617 Size: 32 2791 32 4625 Fragments arranged by size: 4625 2791 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 7480 XbaI T'CTAG_A Cuts at: 3175 4816 3175 Size: 1641 5839 XcmI CCAnnnn_n'nnnnTGG Cuts at: 3919 3919 Size: 7480 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 7480 XmaI C'CCGG_G Cuts at: 1870 4805 1870 Size: 2935 4545 XmnI GAAnn'nnTTC Cuts at: 2418 2478 2546 3380 3710 4341 5609 2418 Size: 60 68 834 330 631 1268 4289 Fragments arranged by size: 4289 1268 834 631 330 68 60 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 5288 276 Size: 53 83 186 936 3754 2468 Fragments arranged by size: 3754 2468 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI EcoRV FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NsiI NspI PacI PasI PciI PflMI PfoI PmeI PpiI PshAI PsiI PspOMI PstI PvuI PvuII SacI SalI SapI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI SwaI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AscI AvrII BaeI BbeI BbvCI BplI BsiWI BsmBI BstZ17I Bsu36I DraIII FseI FspAI KasI NaeI NarI NgoMIV NruI PmlI PpuMI PspXI PsrI RsrII SacII SanDI SexAI SfiI SfoI SrfI