(Circular) (Six-base) MAPSORT of: pFN21AB5666.seq Check: 251 from: 1 to: 7606 pFN21AB5666 7606 bp With 182 enzymes: SgfI * October 20, 2009 10:44 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2348 3573 2348 Size: 1225 6381 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 2361 5416 278 Size: 53 83 186 936 825 3055 2468 Fragments arranged by size: 3055 2468 936 825 186 83 53 AccI GT'mk_AC Cuts at: 1937 4392 4949 1937 Size: 2455 557 4594 Fragments arranged by size: 4594 2455 557 Acc65I G'GTAC_C Cuts at: 4533 4927 4533 Size: 394 7212 AclI AA'CG_TT Cuts at: 1563 5733 6106 1563 Size: 4170 373 3063 Fragments arranged by size: 4170 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3823 4186 4190 5669 7053 7321 1632 Size: 2191 363 4 1479 1384 268 1917 Fragments arranged by size: 2191 1917 1479 1384 363 268 4 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 7409 AflIII A'CryG_T Cuts at: 1203 3750 6505 7482 1203 Size: 2547 2755 977 1327 Fragments arranged by size: 2755 2547 1327 977 AgeI A'CCGG_T Cuts at: 3585 6437 3585 Size: 2852 4754 AhdI GACnn_n'nnGTC Cuts at: 3118 6335 3118 Size: 3217 4389 AleI CACnn'nnGTG Cuts at: 1166 3608 1166 Size: 2442 5164 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2089 1184 Size: 905 6701 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2531 2563 3572 3604 2531 Size: 32 1009 32 6533 Fragments arranged by size: 6533 1009 32 32 AlwNI CAG_nnn'CTG Cuts at: 3502 4756 5349 6921 3502 Size: 1254 593 1572 4187 Fragments arranged by size: 4187 1572 1254 593 ApaI G_GGCC'C Cuts at: 2917 2972 4813 2917 Size: 55 1841 5710 Fragments arranged by size: 5710 1841 55 ApaLI G'TGCA_C Cuts at: 2074 2495 5663 6819 2074 Size: 421 3168 1156 2861 Fragments arranged by size: 3168 2861 1156 421 ApoI r'AATT_y Cuts at: 1493 3740 4915 5141 5293 7402 1493 Size: 2247 1175 226 152 2109 1697 Fragments arranged by size: 2247 2109 1697 1175 226 152 AseI AT'TA_AT Cuts at: 160 1032 3990 6160 160 Size: 872 2958 2170 1606 Fragments arranged by size: 2958 2170 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 7606 AvaI C'yCGr_G Cuts at: 1870 1943 4806 4931 1870 Size: 73 2863 125 4545 Fragments arranged by size: 4545 2863 125 73 BamHI G'GATC_C Cuts at: 2049 4521 4936 5303 2049 Size: 2472 415 367 4352 Fragments arranged by size: 4352 2472 415 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3059 3443 4533 4802 4927 Size: 325 217 627 1272 384 1090 269 125 Cuts at: 4927 6382 618 Size: 1455 1842 Fragments arranged by size: 1842 1455 1272 1090 627 384 325 269 217 125 BanII G_rGCy'C Cuts at: 1391 2321 2478 2510 2917 2972 3285 3826 4813 Size: 930 157 32 407 55 313 541 987 Cuts at: 4813 4925 1391 Size: 112 4072 Fragments arranged by size: 4072 987 930 541 407 313 157 112 55 32 BbeI G_GCGC'C Cuts at: 3063 3447 3063 Size: 384 7222 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2790 4838 928 Size: 971 891 2048 3696 Fragments arranged by size: 3696 2048 971 891 BbvCI CC'TCA_GC Cuts at: 2164 2396 2164 Size: 232 7374 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 2065 2099 4250 4284 4387 4421 5797 Size: 34 128 34 2151 34 103 34 1376 Cuts at: 5797 5831 1903 Size: 34 3678 Fragments arranged by size: 3678 2151 1376 128 103 34 34 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3661 5498 6708 3661 Size: 1837 1210 4559 Fragments arranged by size: 4559 1837 1210 BclI T'GATC_A Cuts at: 1548 1548 Size: 7606 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2693 2933 6217 Size: 107 122 71 631 605 1021 240 3284 Cuts at: 6217 136 Size: 1525 Fragments arranged by size: 3284 1525 1021 631 605 240 122 107 71 BglII A'GATC_T Cuts at: 7601 7601 Size: 7606 BlpI GC'TnA_GC Cuts at: 5023 5023 Size: 7606 Bme1580I G_kGCm'C Cuts at: 1790 2078 2409 2499 2917 2972 3765 3982 4018 Size: 288 331 90 418 55 793 217 36 Cuts at: 4018 4813 5667 6823 1790 Size: 795 854 1156 2573 Fragments arranged by size: 2573 1156 854 795 793 418 331 288 217 90 55 36 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 7606 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2916 3082 5337 Size: 416 53 83 399 24 1499 166 2255 Cuts at: 5337 6295 442 Size: 958 1753 Fragments arranged by size: 2255 1753 1499 958 416 399 166 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 7606 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4336 4336 Size: 7606 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2305 3112 3451 3940 4243 4778 4835 4847 Size: 1066 807 339 489 303 535 57 12 Cuts at: 4847 4865 6266 1239 Size: 18 1401 2579 Fragments arranged by size: 2579 1401 1066 807 535 489 339 303 57 18 12 Bpu10I CC'TnA_GC Cuts at: 2164 2396 4100 4109 4538 4798 2164 Size: 232 1704 9 429 260 4972 Fragments arranged by size: 4972 1704 429 260 232 9 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2486 5731 6596 6894 2486 Size: 3245 865 298 3198 Fragments arranged by size: 3245 3198 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2266 3942 4324 6269 882 Size: 617 767 1676 382 1945 2219 Fragments arranged by size: 2219 1945 1676 767 617 382 BsaAI yAC'GTr Cuts at: 493 1204 2835 2872 3253 3751 5397 493 Size: 711 1631 37 381 498 1646 2702 Fragments arranged by size: 2702 1646 1631 711 498 381 37 BsaBI GATnn'nnATC Cuts at: 2906 7600 2906 Size: 4694 2912 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2358 3060 3423 3444 Size: 53 83 186 936 825 702 363 21 Cuts at: 3444 3506 3996 4578 5413 5795 6430 275 Size: 62 490 582 835 382 635 1451 Fragments arranged by size: 1451 936 835 825 702 635 582 490 382 363 186 83 62 53 21 BsaWI w'CCGG_w Cuts at: 2904 3585 4566 6039 6437 6711 6858 7269 2904 Size: 681 981 1473 398 274 147 411 3241 Fragments arranged by size: 3241 1473 981 681 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 3413 3443 4412 4442 1178 Size: 30 165 30 2010 30 969 30 4342 Fragments arranged by size: 4342 2010 969 165 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 3115 3346 4325 4436 4439 1186 Size: 1929 231 979 111 3 4353 Fragments arranged by size: 4353 1929 979 231 111 3 BseYI C'CCAG_C Cuts at: 2439 2765 6809 7305 2439 Size: 326 4044 496 2740 Fragments arranged by size: 4044 2740 496 326 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 7033 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 5056 5817 6845 1540 Size: 275 186 3055 761 1028 2301 Fragments arranged by size: 3055 2301 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2078 2499 3699 4881 5667 5752 6823 2078 Size: 421 1200 1182 786 85 1071 2861 Fragments arranged by size: 2861 1200 1182 1071 786 421 85 BsmI GAATG_Cn' Cuts at: 5117 5210 5117 Size: 93 7513 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2078 2321 2409 2478 2499 2510 2917 Size: 399 288 243 88 69 21 11 407 Cuts at: 2917 2972 3285 3699 3765 3826 3982 4018 4813 Size: 55 313 414 66 61 156 36 795 Cuts at: 4813 4881 4925 5667 5752 6823 1391 Size: 68 44 742 85 1071 2174 Fragments arranged by size: 2174 1071 795 742 414 407 399 313 288 243 156 88 85 69 68 66 61 55 44 36 21 11 BspEI T'CCGG_A Cuts at: 2904 4566 7269 2904 Size: 1662 2703 3241 Fragments arranged by size: 3241 2703 1662 BspHI T'CATG_A Cuts at: 3166 3621 5495 7225 3166 Size: 455 1874 1730 3547 Fragments arranged by size: 3547 1874 1730 455 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2348 3573 4168 4386 4961 844 Size: 1504 1225 595 218 575 3489 Fragments arranged by size: 3489 1504 1225 595 575 218 BsrBI CCG'CTC Cuts at: 1394 1823 3145 4137 5493 1394 Size: 429 1322 992 1356 3507 Fragments arranged by size: 3507 1356 1322 992 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2614 2928 3859 6101 6275 65 Size: 1183 1366 314 931 2242 174 1396 Fragments arranged by size: 2242 1396 1366 1183 931 314 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2683 3056 3397 3585 6250 6437 1700 Size: 110 873 373 341 188 2665 187 2869 Fragments arranged by size: 2869 2665 873 373 341 188 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 7606 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 7606 BssSI C'ACGA_G Cuts at: 5666 6678 5666 Size: 1012 6594 BstAPI GCAn_nnn'nTGC Cuts at: 794 3344 4284 794 Size: 2550 940 4116 Fragments arranged by size: 4116 2550 940 BstBI TT'CG_AA Cuts at: 6410 6410 Size: 7606 BstEII G'GTnAC_C Cuts at: 1184 3082 3199 1184 Size: 1898 117 5591 Fragments arranged by size: 5591 1898 117 BstXI CCAn_nnnn'nTGG Cuts at: 2035 2197 2035 Size: 162 7444 BstYI r'GATC_y Cuts at: 1972 2049 2907 4230 4521 4936 5303 5688 5705 Size: 77 858 1323 291 415 367 385 17 Cuts at: 5705 7157 7243 7255 7601 1972 Size: 1452 86 12 346 1977 Fragments arranged by size: 1977 1452 1323 858 415 385 367 346 291 86 77 17 12 Bsu36I CC'TnA_GG Cuts at: 3455 3455 Size: 7606 BtgI C'CryG_G Cuts at: 513 1065 2004 2139 2941 3063 513 Size: 552 939 135 802 122 5056 Fragments arranged by size: 5056 939 802 552 135 122 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2068 487 Size: 683 56 842 6025 Fragments arranged by size: 6025 842 683 56 BtsI GCAGTG_nn' Cuts at: 3345 4343 4722 5122 5916 5936 3345 Size: 998 379 400 794 20 5015 Fragments arranged by size: 5015 998 794 400 379 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 7606 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 7571 DraI TTT'AAA Cuts at: 4910 4910 Size: 7606 DraIII CAC_nnn'GTG Cuts at: 2413 2413 Size: 7606 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 2740 6613 809 Size: 507 1424 3873 1802 Fragments arranged by size: 3873 1802 1424 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2007 2187 2625 Size: 54 1425 50 275 18 177 180 438 Cuts at: 2625 2685 3957 4008 5053 5942 7571 8 Size: 60 1272 51 1045 889 1629 43 Fragments arranged by size: 1629 1425 1272 1045 889 438 275 180 177 60 54 51 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 5053 1537 Size: 275 3241 4090 Fragments arranged by size: 4090 3241 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3842 3906 4061 4062 4196 5536 1353 Size: 2489 64 155 1 134 1340 3423 Fragments arranged by size: 3423 2489 1340 155 134 64 1 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2149 6193 6567 6713 387 Size: 1064 138 560 4044 374 146 1280 Fragments arranged by size: 4044 1280 1064 560 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2305 3112 3451 3823 3940 4186 4190 Size: 393 673 807 339 372 117 246 4 Cuts at: 4190 4243 4778 4835 4847 4865 5669 6266 7053 Size: 53 535 57 12 18 804 597 787 Cuts at: 7053 7321 1239 Size: 268 1524 Fragments arranged by size: 1524 807 804 787 673 597 535 393 372 339 268 246 117 57 53 18 12 4 EcoNI CCTnn'n_nnAGG Cuts at: 2399 2516 3479 3569 3689 4709 2399 Size: 117 963 90 120 1020 5296 Fragments arranged by size: 5296 1020 963 120 117 90 EcoO109I rG'GnC_Cy Cuts at: 2299 2728 2762 2969 3499 3681 3862 4024 4810 Size: 429 34 207 530 182 181 162 786 Cuts at: 4810 2299 Size: 5095 Fragments arranged by size: 5095 786 530 429 207 182 181 162 34 EcoRI G'AATT_C Cuts at: 3740 4915 5293 3740 Size: 1175 378 6053 Fragments arranged by size: 6053 1175 378 FspI TGC'GCA Cuts at: 6112 6112 Size: 7606 HaeII r_GCGC'y Cuts at: 1678 3063 3447 4043 6753 1678 Size: 1385 384 596 2710 2531 Fragments arranged by size: 2710 2531 1385 596 384 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2265 2297 3104 3136 3413 Size: 32 135 32 693 32 807 32 277 Cuts at: 3413 3445 3919 3951 4568 4600 6247 6279 6321 Size: 32 474 32 617 32 1647 32 42 Cuts at: 6321 6353 1373 Size: 32 2626 Fragments arranged by size: 2626 1647 807 693 617 474 277 135 42 32 32 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 4950 5196 669 Size: 988 281 3012 246 3079 Fragments arranged by size: 3079 3012 988 281 246 HindIII A'AGCT_T Cuts at: 748 4661 748 Size: 3913 3693 HpaI GTT'AAC Cuts at: 5196 5196 Size: 7606 KasI G'GCGC_C Cuts at: 3059 3443 3059 Size: 384 7222 KpnI G_GTAC'C Cuts at: 4537 4931 4537 Size: 394 7212 MfeI C'AATT_G Cuts at: 5205 5205 Size: 7606 MluI A'CGCG_T Cuts at: 7482 7482 Size: 7606 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2769 3619 4314 4725 6720 6904 1305 Size: 1464 850 695 411 1995 184 2007 Fragments arranged by size: 2007 1995 1464 850 695 411 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2009 2627 4010 10 Size: 54 1425 343 177 618 1383 3606 Fragments arranged by size: 3606 1425 1383 618 343 177 54 MslI CAynn'nnrTG Cuts at: 518 1166 3608 3662 5564 5923 6082 518 Size: 648 2442 54 1902 359 159 2042 Fragments arranged by size: 2442 2042 1902 648 359 159 54 MspA1I CmG'CkG Cuts at: 1606 3367 3630 3940 4756 5022 5699 6847 7092 Size: 1761 263 310 816 266 677 1148 245 Cuts at: 7092 1606 Size: 2120 Fragments arranged by size: 2120 1761 1148 816 677 310 266 263 245 NaeI GCC'GGC Cuts at: 2685 3058 3399 2685 Size: 373 341 6892 Fragments arranged by size: 6892 373 341 NarI GG'CG_CC Cuts at: 3060 3444 3060 Size: 384 7222 NcoI C'CATG_G Cuts at: 513 1065 2004 3063 513 Size: 552 939 1059 5056 Fragments arranged by size: 5056 1059 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 7606 NgoMIV G'CCGG_C Cuts at: 2683 3056 3397 2683 Size: 373 341 6892 Fragments arranged by size: 6892 373 341 NheI G'CTAG_C Cuts at: 1052 1052 Size: 7606 NotI GC'GGCC_GC Cuts at: 5053 5053 Size: 7606 NspI r_CATG'y Cuts at: 1132 1229 2752 4084 4964 6509 1132 Size: 97 1523 1332 880 1545 2229 Fragments arranged by size: 2229 1545 1523 1332 880 97 PasI CC'CwG_GG Cuts at: 1348 2035 4086 4411 1348 Size: 687 2051 325 4543 Fragments arranged by size: 4543 2051 687 325 PciI A'CATG_T Cuts at: 6505 6505 Size: 7606 PflMI CCAn_nnn'nTGG Cuts at: 1108 3210 4221 1108 Size: 2102 1011 4493 Fragments arranged by size: 4493 2102 1011 PmeI GTTT'AAAC Cuts at: 4910 4910 Size: 7606 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 5675 5707 7188 7220 5675 Size: 32 1481 32 6061 Fragments arranged by size: 6061 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2728 2762 3681 3862 4024 2728 Size: 34 919 181 162 6310 Fragments arranged by size: 6310 919 181 162 34 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 7606 PsiI TTA'TAA Cuts at: 5176 5176 Size: 7606 PspOMI G'GGCC_C Cuts at: 2913 2968 4809 2913 Size: 55 1841 5710 Fragments arranged by size: 5710 1841 55 PsrI GAACnnnnnnTACnnnnnnn_nnnnn' Cuts at: 3527 3559 3527 Size: 32 7574 PstI C_TGCA'G Cuts at: 830 2650 3570 3642 3945 4508 4752 4958 830 Size: 1820 920 72 303 563 244 206 3478 Fragments arranged by size: 3478 1820 920 563 303 244 206 72 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 7606 PvuII CAG'CTG Cuts at: 3940 4756 3940 Size: 816 6790 SalI G'TCGA_C Cuts at: 1936 4948 1936 Size: 3012 4594 SanDI GG'GwC_CC Cuts at: 3681 3681 Size: 7606 SbfI CC_TGCA'GG Cuts at: 3570 4958 3570 Size: 1388 6218 ScaI AGT'ACT Cuts at: 5854 7566 5854 Size: 1712 5894 SexAI A'CCwGG_T Cuts at: 2487 3195 2487 Size: 708 6898 SfcI C'TryA_G Cuts at: 826 1045 2646 3433 3566 3638 3941 4504 4748 Size: 219 1601 787 133 72 303 563 244 Cuts at: 4748 4954 6089 6770 6961 826 Size: 206 1135 681 191 1471 Fragments arranged by size: 1601 1471 1135 787 681 563 303 244 219 206 191 133 72 SfiI GGCCn_nnn'nGGCC Cuts at: 2693 2693 Size: 7606 SfoI GGC'GCC Cuts at: 3061 3445 3061 Size: 384 7222 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 7606 SgrAI Cr'CCGG_yG Cuts at: 1700 3056 1700 Size: 1356 6250 SmaI CCC'GGG Cuts at: 1872 4808 4933 1872 Size: 2936 125 4545 Fragments arranged by size: 4545 2936 125 SmlI C'TyrA_G Cuts at: 820 1017 1943 2501 5710 6611 6873 820 Size: 197 926 558 3209 901 262 1553 Fragments arranged by size: 3209 1553 926 901 558 262 197 SnaBI TAC'GTA Cuts at: 493 5397 493 Size: 4904 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 7606 SphI G_CATG'C Cuts at: 1132 4964 1132 Size: 3832 3774 SspI AAT'ATT Cuts at: 5 52 5530 5 Size: 47 5478 2081 Fragments arranged by size: 5478 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 7606 StyI C'CwwG_G Cuts at: 513 1065 2004 2010 2196 2724 3063 5045 513 Size: 552 939 6 186 528 339 1982 3074 Fragments arranged by size: 3074 1982 939 552 528 339 186 6 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3254 4843 5633 5650 5803 5988 6433 Size: 1255 1674 1589 790 17 153 185 445 Cuts at: 6433 325 Size: 1498 Fragments arranged by size: 1674 1589 1498 1255 790 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2100 2595 4077 Size: 274 80 33 51 1443 123 495 1482 Cuts at: 4077 4254 5852 7564 96 Size: 177 1598 1712 138 Fragments arranged by size: 1712 1598 1482 1443 495 274 177 138 123 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2761 2793 3353 3385 4412 4444 7566 7598 2761 Size: 32 560 32 1027 32 3122 32 2769 Fragments arranged by size: 3122 2769 1027 560 32 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 4640 1316 Size: 3324 4282 XbaI T'CTAG_A Cuts at: 4942 4942 Size: 7606 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2485 2839 2485 Size: 354 7252 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 7606 XmaI C'CCGG_G Cuts at: 1870 4806 4931 1870 Size: 2936 125 4545 Fragments arranged by size: 4545 2936 125 XmnI GAAnn'nnTTC Cuts at: 2663 5735 2663 Size: 3072 4534 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 2359 5414 276 Size: 53 83 186 936 825 3055 2468 Fragments arranged by size: 3055 2468 936 825 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI PspOMI PsrI PstI PvuI PvuII SalI SanDI SbfI ScaI SexAI SfcI SfiI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AscI AvrII BaeI BfrBI BsiWI BsmBI BstZ17I EcoICRI EcoRV FalI FseI FspAI NruI NsiI PacI PfoI PmlI PspXI RsrII SacI SacII SapI SrfI SwaI