Restriction map for
pFN21AB5662
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# NO CUT ENZYMES ( 119 enzymes) #
##################################
AatII Acc65I AccI AclI AflII
AflIII AgeI AhdI Alw44I AlwNI
AocI Aor51HI ApaLI AscI AseI
AsnI Asp700I Asp718I AspEI AspI
AviII AvrII BalI BamHI BbrPI
BcgI BciVI BclI BfrI BglII
BlnI BlpI Bpu1102I BsaBI BsaI
BsiEI BsiWI Bsp1407I BspDI BspHI
BspLU11I BsrDI BsrGI BssSI Bst1107I
BstAPI BstBI BstEII BstPI BstZ17I
Bsu36I CelII ClaI DraI DraIII
EagI Eam1105I EclXI Eco47III Eco52I
Eco81I EcoNI EcoO65I EcoRI EcoRV
EcoT22I FbaI FseI FspI HincII
HindII HpaI KpnI MamI MfeI
MluI MluNI MscI MunI NcoI
NdeI NheI NotI NruI NsiI
NspV PacI PaeR7I PflFI PflMI
PinAI PmaCI PmeI PmlI PshAI
PshBI Psp1406I PvuI RcaI SacI
SalI SbfI ScaI SexAI SfiI
SfuI SgrAI SnaBI SpeI SphI
Sse8387I SspBI SspI SwaI TfiI
Tth111I Van91I XhoI XmnI
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# ONE CUT ENZYMES ( 47 enzymes) #
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AccIII 781 TCCGGA T^CCGGA
AcsI 884 AAATTC [GA]^AATT[CT]
ApaI 259 GGGCCC GGGCC^C
ApoI 884 AAATTC [GA]^AATT[CT]
BbeI 34 GGCGCC GGCGC^C
BbvCI 177 CCTCAGC CCTCAGC(-5/-2)
BglI 182 GCCCCCCCGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BpmI 159 CTCCAG CTGGAG(16/14)
BsaAI 742 TACGTG [CT]AC^GT[GA]
BseAI 781 TCCGGA T^CCGGA
BseRI 646 GAGGAG GAGGAG(10/8)
BsgI 1000 CTGCAC GTGCAG(16/14)
BsmBI 957 GAGACG CGTCTC(1/5)
BsmI 905 GCATTC GAATGC(1/-1)
BspEI 781 TCCGGA T^CCGGA
BspMI 281 ACCTGC ACCTGC(4/8)
BsrFI 232 GCCGGC [GA]^CCGG[CT]
BssHII 251 GCGCGC G^CGCGC
BstXI 599 CCATCGTGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Cfr10I 232 GCCGGC [GA]^CCGG[CT]
CpoI 9 CGGTCCG CG^G[AT]CCG
DrdI 665 GACTAGAAAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
HindIII 619 AAGCTT A^AGCTT
HphI 813 GGTGA GGTGA(8/7)
KasI 34 GGCGCC G^GCGCC
KspI 48 CCGCGG CCGC^GG
MaeIII 1094 GTGAC ^GT[ACGT]AC
MroI 781 TCCGGA T^CCGGA
MseI 371 TTAA T^TAA
MslI 502 CACTCCTATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
NaeI 232 GCCGGC GCC^GGC
NarI 34 GGCGCC GG^CGCC
NgoMIV 232 GCCGGC G^CCGGC
PstI 198 CTGCAG CTGCA^G
PvuII 406 CAGCTG CAG^CTG
RsrII 9 CGGTCCG CG^G[AT]CCG
SacII 48 CCGCGG CCGC^GG
SfcI 198 CTGCAG C^T[GA][CT]AG
SfoI 34 GGCGCC GGC^GCC
SmlI 954 CTTGAG C^T[CT][GA]AG
TaqI 1040 TCGA T^CGA
Tru9I 371 TTAA T^TAA
Tsp45I 1094 GTGAC ^GT[GC]AC
Tsp509I 885 AATT ^AATT
TthHB8I 1040 TCGA T^CGA
XbaI 675 TCTAGA T^CTAGA
XcmI 92 CCAAACGCCTGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
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# TWO CUT ENZYMES ( 26 enzymes) #
###################################
AfaI 1035 GTAC GT^AC
1080 GTAC
AspHI 156 GTGCTC G[AT]GC[AT]^C
913 GAGCAC
BanI 34 GGCGCC G^G[CT][GA]CC
935 GGTGCC
BsaWI 12 TCCGGT [AT]^CCGG[AT]
781 TCCGGA
BsiHKAI 156 GTGCTC G[AT]GC[AT]^C
913 GAGCAC
BsrBI 221 CCGCTC CCGCTC(-3/-3)
30 GAGCGG
BstF5I 330 CATCC GGATG(2/0)
402 CATCC
BstYI 755 AGATCC [GA]^GATC[CT]
992 AGATCC
DsaI 48 CCGCGG C^C[GA][CT]GG
641 CCACGG
EaeI 89 CGGCCA [CT]^GGCC[GA]
131 CGGCCA
EcoT14I 450 CCTTGG C^C[AT][AT]GG
461 CCAAGG
FokI 330 CATCC GGATG(9/13)
402 CATCC
HaeII 34 GGCGCC [GA]GCGC^[CT]
321 GGCGCT
MaeII 743 ACGT A^CGT
797 ACGT
MflI 755 AGATCC [GA]^GATC[CT]
992 AGATCC
MspA1I 48 CCGCGG C[AC]G^C[GT]G
406 CAGCTG
NspI 309 GCATGT [GA]CATG^[CT]
497 ACATGC
PpuMI 611 GGGACCT [GA]G^G[AT]CC[CT]
772 GGGACCT
RsaI 1035 GTAC GT^AC
1080 GTAC
SapI 63 GCTCTTC GCTCTTC(1/4)
552 GCTCTTC
SmaI 317 CCCGGG CCC^GGG
1030 CCCGGG
StuI 394 AGGCCT AGG^CCT
464 AGGCCT
StyI 450 CCTTGG C^C[AT][AT]GG
461 CCAAGG
XhoII 755 AGATCC [GA]^GATC[CT]
992 AGATCC
XmaCI 317 CCCGGG C^CCGGG
1030 CCCGGG
XmaI 317 CCCGGG C^CCGGG
1030 CCCGGG
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# 3 OR MORE CUT ENZYMES ( 66 enzymes) #
#########################################
AccII AciI AcyI AluI AlwI
AvaI AvaII BanII BbsI BbvI
BcnI BfaI BmyI BpuAI BsaHI
BsaJI BsiYI BslI BsmAI BsmFI
BsoBI Bsp1286I BsrI BssKI BstNI
BstUI Cac8I CfoI Cfr13I DdeI
DpnII DraII EarI EcoO109I EcoRII
FauI Fnu4HI HaeIII HapII HgaI
HhaI Hin1I HinP1I HinfI HpaII
ItaI Ksp632I MaeI MboI MboII
MnlI MspI MvaI MvnI MwoI
NciI NdeII NlaIII NlaIV PleI
Sau3AI Sau96I ScrFI SfaNI TseI
TspRI