(Circular) (Six-base) MAPSORT of: pFN21AB5662.seq Check: 7520 from: 1 to: 5821 pFN21AB5662 5821 bp With 182 enzymes: SgfI * February 27, 2009 14:34 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3631 278 Size: 53 83 186 936 2095 2468 Fragments arranged by size: 2468 2095 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3164 1937 Size: 1227 4594 Acc65I G'GTAC_C Cuts at: 3142 3142 Size: 5821 AclI AA'CG_TT Cuts at: 1563 3948 4321 1563 Size: 2385 373 3063 Fragments arranged by size: 3063 2385 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2642 3048 3884 5268 5536 1632 Size: 1010 406 836 1384 268 1917 Fragments arranged by size: 1917 1384 1010 836 406 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5624 AflIII A'CryG_T Cuts at: 1203 4720 5697 1203 Size: 3517 977 1327 Fragments arranged by size: 3517 1327 977 AgeI A'CCGG_T Cuts at: 4652 4652 Size: 5821 AhdI GACnn_n'nnGTC Cuts at: 4550 4550 Size: 5821 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5821 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5821 AlwNI CAG_nnn'CTG Cuts at: 3564 5136 3564 Size: 1572 4249 ApaI G_GGCC'C Cuts at: 2268 2268 Size: 5821 ApaLI G'TGCA_C Cuts at: 3878 5034 3878 Size: 1156 4665 ApoI r'AATT_y Cuts at: 1493 2889 3130 3356 3508 5617 1493 Size: 1396 241 226 152 2109 1697 Fragments arranged by size: 2109 1697 1396 241 226 152 AseI AT'TA_AT Cuts at: 160 1032 4375 160 Size: 872 3343 1606 Fragments arranged by size: 3343 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5821 AvaI C'yCGr_G Cuts at: 1870 1943 2082 2322 2993 3035 3146 1870 Size: 73 139 240 671 42 111 4545 Fragments arranged by size: 4545 671 240 139 111 73 42 BamHI G'GATC_C Cuts at: 3151 3518 3151 Size: 367 5454 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2039 2940 3142 4597 618 Size: 325 217 627 252 901 202 1455 1842 Fragments arranged by size: 1842 1455 901 627 325 252 217 202 BanII G_rGCy'C Cuts at: 1391 2268 2756 2973 3140 1391 Size: 877 488 217 167 4072 Fragments arranged by size: 4072 877 488 217 167 BbeI G_GCGC'C Cuts at: 2043 2043 Size: 5821 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2470 2632 2691 928 Size: 971 571 162 59 4058 Fragments arranged by size: 4058 971 571 162 59 BbvCI CC'TCA_GC Cuts at: 2183 2183 Size: 5821 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 1987 2021 4012 4046 1903 Size: 34 50 34 1991 34 3678 Fragments arranged by size: 3678 1991 50 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3713 4923 3713 Size: 1210 4611 BclI T'GATC_A Cuts at: 1548 1548 Size: 5821 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2193 4432 136 Size: 107 122 71 631 605 521 2239 1525 Fragments arranged by size: 2239 1525 631 605 521 122 107 71 BglII A'GATC_T Cuts at: 5816 5816 Size: 5821 BlpI GC'TnA_GC Cuts at: 3238 3238 Size: 5821 Bme1580I G_kGCm'C Cuts at: 1790 2147 2268 2878 3882 5038 1790 Size: 357 121 610 1004 1156 2573 Fragments arranged by size: 2573 1156 1004 610 357 121 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5821 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2784 2865 3552 Size: 416 53 83 399 24 1367 81 687 Cuts at: 3552 4510 442 Size: 958 1753 Fragments arranged by size: 1753 1367 958 687 416 399 83 81 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5821 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2149 4481 1239 Size: 910 2332 2579 Fragments arranged by size: 2579 2332 910 Bpu10I CC'TnA_GC Cuts at: 2183 2627 2916 2183 Size: 444 289 5088 Fragments arranged by size: 5088 444 289 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2980 3946 4811 5109 2980 Size: 966 865 298 3692 Fragments arranged by size: 3692 966 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4484 882 Size: 617 2985 2219 Fragments arranged by size: 2985 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 2749 3612 493 Size: 711 1545 863 2702 Fragments arranged by size: 2702 1545 863 711 BsaBI GATnn'nnATC Cuts at: 5815 5815 Size: 5821 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2040 2088 2112 3628 Size: 53 83 186 936 507 48 24 1516 Cuts at: 3628 4010 4645 275 Size: 382 635 1451 Fragments arranged by size: 1516 1451 936 635 507 382 186 83 53 48 24 BsaWI w'CCGG_w Cuts at: 2017 2786 4254 4652 4926 5073 5484 2017 Size: 769 1468 398 274 147 411 2354 Fragments arranged by size: 2354 1468 769 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2492 2522 1178 Size: 30 165 30 1089 30 4477 Fragments arranged by size: 4477 1089 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2666 1186 Size: 1480 4341 BseYI C'CCAG_C Cuts at: 2074 2267 2611 2661 2740 5024 5520 2074 Size: 193 344 50 79 2284 496 2375 Fragments arranged by size: 2375 2284 496 344 193 79 50 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2990 1163 Size: 573 1254 3994 Fragments arranged by size: 3994 1254 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3271 4032 5060 1540 Size: 275 186 1270 761 1028 2301 Fragments arranged by size: 2301 1270 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2165 2922 3882 3967 5038 2165 Size: 757 960 85 1071 2948 Fragments arranged by size: 2948 1071 960 757 85 BsmI GAATG_Cn' Cuts at: 2910 3332 3425 2910 Size: 422 93 5306 Fragments arranged by size: 5306 422 93 BsmBI CGTCTCn'nnnn_ Cuts at: 2956 2956 Size: 5821 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2147 2165 2268 2756 2878 2922 2973 Size: 399 357 18 103 488 122 44 51 Cuts at: 2973 3140 3882 3967 5038 1391 Size: 167 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 488 399 357 167 122 103 85 51 44 18 BspEI T'CCGG_A Cuts at: 2786 5484 2786 Size: 2698 3123 BspHI T'CATG_A Cuts at: 3710 5440 3710 Size: 1730 4091 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2295 3176 844 Size: 1451 881 3489 Fragments arranged by size: 3489 1451 881 BsrBI CCG'CTC Cuts at: 1394 1823 2037 2228 3708 1394 Size: 429 214 191 1480 3507 Fragments arranged by size: 3507 1480 429 214 191 BsrDI GCAATG_nn' Cuts at: 65 1248 4316 4490 65 Size: 1183 3068 174 1396 Fragments arranged by size: 3068 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2237 4465 4652 1700 Size: 110 427 2228 187 2869 Fragments arranged by size: 2869 2228 427 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5821 BssHII G'CGCG_C Cuts at: 1925 2256 1925 Size: 331 5490 BssSI C'ACGA_G Cuts at: 3881 4893 3881 Size: 1012 4809 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5821 BstBI TT'CG_AA Cuts at: 4625 4625 Size: 5821 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5821 BstXI CCAn_nnnn'nTGG Cuts at: 2611 2611 Size: 5821 BstYI r'GATC_y Cuts at: 1972 2760 2997 3151 3518 3903 3920 5372 5458 Size: 788 237 154 367 385 17 1452 86 Cuts at: 5458 5470 5816 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1452 788 385 367 346 237 154 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2053 2646 513 Size: 552 988 593 3688 Fragments arranged by size: 3688 988 593 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2260 2403 487 Size: 683 56 1034 143 3905 Fragments arranged by size: 3905 1034 683 143 56 BtsI GCAGTG_nn' Cuts at: 2587 2985 3337 4131 4151 2587 Size: 398 352 794 20 4257 Fragments arranged by size: 4257 794 398 352 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5821 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5786 DraI TTT'AAA Cuts at: 3125 3125 Size: 5821 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 2676 4828 809 Size: 507 1360 2152 1802 Fragments arranged by size: 2152 1802 1360 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2094 2136 3268 Size: 54 1425 50 275 18 264 42 1132 Cuts at: 3268 4157 5786 8 Size: 889 1629 43 Fragments arranged by size: 1629 1425 1132 889 275 264 54 50 43 42 18 EagI C'GGCC_G Cuts at: 1537 1812 3268 1537 Size: 275 1456 4090 Fragments arranged by size: 4090 1456 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2075 2522 2535 2564 3751 1353 Size: 722 447 13 29 1187 3423 Fragments arranged by size: 3423 1187 722 447 29 13 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2256 2566 4408 4782 4928 387 Size: 1064 138 667 310 1842 374 146 1280 Fragments arranged by size: 1842 1280 1064 667 374 310 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2149 2642 3048 3884 4481 5268 5536 Size: 393 517 493 406 836 597 787 268 Cuts at: 5536 1239 Size: 1524 Fragments arranged by size: 1524 836 787 597 517 493 406 393 268 EcoO109I rG'GnC_Cy Cuts at: 2264 2617 2778 2264 Size: 353 161 5307 Fragments arranged by size: 5307 353 161 EcoRI G'AATT_C Cuts at: 3130 3508 3130 Size: 378 5443 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2471 2656 2471 Size: 185 5636 FspI TGC'GCA Cuts at: 4327 4327 Size: 5821 HaeII r_GCGC'y Cuts at: 1678 2043 2330 4968 1678 Size: 365 287 2638 2531 Fragments arranged by size: 2638 2531 365 287 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2303 2335 2492 2524 2705 Size: 32 135 32 731 32 157 32 181 Cuts at: 2705 2737 4462 4494 4536 4568 1373 Size: 32 1725 32 42 32 2626 Fragments arranged by size: 2626 1725 731 181 157 135 42 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3165 3411 669 Size: 988 281 1227 246 3079 Fragments arranged by size: 3079 1227 988 281 246 HindIII A'AGCT_T Cuts at: 748 2624 748 Size: 1876 3945 HpaI GTT'AAC Cuts at: 3411 3411 Size: 5821 KasI G'GCGC_C Cuts at: 2039 2039 Size: 5821 KpnI G_GTAC'C Cuts at: 3146 3146 Size: 5821 MfeI C'AATT_G Cuts at: 3420 3420 Size: 5821 MluI A'CGCG_T Cuts at: 5697 5697 Size: 5821 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2821 4935 5119 1305 Size: 1516 2114 184 2007 Fragments arranged by size: 2114 2007 1516 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 3999 Fragments arranged by size: 3999 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2511 3779 4138 4297 518 Size: 648 1345 1268 359 159 2042 Fragments arranged by size: 2042 1345 1268 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2055 2413 3237 3914 5062 5307 1606 Size: 449 358 824 677 1148 245 2120 Fragments arranged by size: 2120 1148 824 677 449 358 245 NaeI GCC'GGC Cuts at: 2239 2239 Size: 5821 NarI GG'CG_CC Cuts at: 2040 2040 Size: 5821 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 5269 NdeI CA'TA_TG Cuts at: 387 387 Size: 5821 NgoMIV G'CCGG_C Cuts at: 2237 2237 Size: 5821 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5821 NotI GC'GGCC_GC Cuts at: 3268 3268 Size: 5821 NspI r_CATG'y Cuts at: 1132 1229 2318 2506 3179 4724 1132 Size: 97 1089 188 673 1545 2229 Fragments arranged by size: 2229 1545 1089 673 188 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5821 PciI A'CATG_T Cuts at: 4720 4720 Size: 5821 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5821 PfoI T'CCnGG_A Cuts at: 2249 2249 Size: 5821 PmeI GTTT'AAAC Cuts at: 3125 3125 Size: 5821 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3890 3922 5403 5435 3890 Size: 32 1481 32 4276 Fragments arranged by size: 4276 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2617 2778 2617 Size: 161 5660 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5821 PsiI TTA'TAA Cuts at: 3391 3391 Size: 5821 PspOMI G'GGCC_C Cuts at: 2264 2264 Size: 5821 PstI C_TGCA'G Cuts at: 830 2207 3173 830 Size: 1377 966 3478 Fragments arranged by size: 3478 1377 966 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5821 PvuII CAG'CTG Cuts at: 2413 2413 Size: 5821 RsrII CG'GwC_CG Cuts at: 2015 2015 Size: 5821 SacII CC_GC'GG Cuts at: 2056 2056 Size: 5821 SalI G'TCGA_C Cuts at: 1936 3163 1936 Size: 1227 4594 SapI GCTCTTCn'nnn_ Cuts at: 2075 2564 2075 Size: 489 5332 SbfI CC_TGCA'GG Cuts at: 3173 3173 Size: 5821 ScaI AGT'ACT Cuts at: 4069 5781 4069 Size: 1712 4109 SfcI C'TryA_G Cuts at: 826 1045 2203 3169 4304 4985 5176 826 Size: 219 1158 966 1135 681 191 1471 Fragments arranged by size: 1471 1158 1135 966 681 219 191 SfoI GGC'GCC Cuts at: 2041 2041 Size: 5821 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5821 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5821 SmaI CCC'GGG Cuts at: 1872 2324 3037 3148 1872 Size: 452 713 111 4545 Fragments arranged by size: 4545 713 452 111 SmlI C'TyrA_G Cuts at: 820 1017 1943 2959 3925 4826 5088 820 Size: 197 926 1016 966 901 262 1553 Fragments arranged by size: 1553 1016 966 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3612 493 Size: 3119 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5821 SphI G_CATG'C Cuts at: 1132 3179 1132 Size: 2047 3774 SspI AAT'ATT Cuts at: 5 52 3745 5 Size: 47 3693 2081 Fragments arranged by size: 3693 2081 47 StuI AGG'CCT Cuts at: 1517 2401 2471 1517 Size: 884 70 4867 Fragments arranged by size: 4867 884 70 StyI C'CwwG_G Cuts at: 513 1065 2455 2466 3260 513 Size: 552 1390 11 794 3074 Fragments arranged by size: 3074 1390 794 552 11 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2003 3848 3865 4018 4203 4648 325 Size: 1255 423 1845 17 153 185 445 1498 Fragments arranged by size: 1845 1498 1255 445 423 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 4067 5779 96 Size: 274 80 33 51 1443 2090 1712 138 Fragments arranged by size: 2090 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5781 5813 5781 Size: 32 5789 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5821 XbaI T'CTAG_A Cuts at: 2680 3157 2680 Size: 477 5344 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2104 2104 Size: 5821 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5821 XmaI C'CCGG_G Cuts at: 1870 2322 3035 3146 1870 Size: 452 713 111 4545 Fragments arranged by size: 4545 713 452 111 XmnI GAAnn'nnTTC Cuts at: 3950 3950 Size: 5821 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3629 276 Size: 53 83 186 936 2095 2468 Fragments arranged by size: 2468 2095 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII RsrII SacII SalI SapI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI AscI AvrII BaeI BfrBI BplI BsiWI BstZ17I Bsu36I DraIII EcoICRI EcoNI EcoRV FseI FspAI NruI NsiI PacI PmlI PspXI PsrI SacI SanDI SexAI SfiI SrfI SwaI