Restriction map for
pFN21AB5662
(Linear) (Six-base) MAPSORT of: KIBB5662.seq Check: 9812 from: 1 to: 1116
KIBB5662.orf
With 182 enzymes: SgfI *
February 28, 2007 11:28 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 637 1043 1116
Size: 637 406 73
Fragments arranged by size:
637 406 73
ApaI G_GGCC'C
Cuts at: 0 263 1116
Size: 263 853
ApoI r'AATT_y
Cuts at: 0 884 1116
Size: 884 232
AvaI C'yCGr_G
Cuts at: 0 77 317 988 1030 1116
Size: 77 240 671 42 86
Fragments arranged by size:
671 240 86 77 42
BanI G'GyrC_C
Cuts at: 0 34 935 1116
Size: 34 901 181
Fragments arranged by size:
901 181 34
BanII G_rGCy'C
Cuts at: 0 263 751 968 1116
Size: 263 488 217 148
Fragments arranged by size:
488 263 217 148
BbeI G_GCGC'C
Cuts at: 0 38 1116
Size: 38 1078
BbsI GAAGACnn'nnnn_
Cuts at: 0 465 627 686 1116
Size: 465 162 59 430
Fragments arranged by size:
465 430 162 59
BbvCI CC'TCA_GC
Cuts at: 0 178 1116
Size: 178 938
BglI GCCn_nnn'nGGC
Cuts at: 0 188 1116
Size: 188 928
Bme1580I G_kGCm'C
Cuts at: 0 142 263 873 1116
Size: 142 121 610 243
Fragments arranged by size:
610 243 142 121
BmrI ACTGGGnnnn_n'
Cuts at: 0 779 860 1116
Size: 779 81 256
Fragments arranged by size:
779 256 81
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 144 1116
Size: 144 972
Bpu10I CC'TnA_GC
Cuts at: 0 178 622 911 1116
Size: 178 444 289 205
Fragments arranged by size:
444 289 205 178
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 975 1116
Size: 975 141
BsaAI yAC'GTr
Cuts at: 0 744 1116
Size: 744 372
BsaHI Gr'CG_yC
Cuts at: 0 35 83 107 1116
Size: 35 48 24 1009
Fragments arranged by size:
1009 48 35 24
BsaWI w'CCGG_w
Cuts at: 0 12 781 1116
Size: 12 769 335
Fragments arranged by size:
769 335 12
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 487 517 1116
Size: 487 30 599
Fragments arranged by size:
599 487 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 661 1116
Size: 661 455
BseYI C'CCAG_C
Cuts at: 0 69 262 606 656 735 1116
Size: 69 193 344 50 79 381
Fragments arranged by size:
381 344 193 79 69 50
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 985 1116
Size: 985 131
BsiHKAI G_wGCw'C
Cuts at: 0 160 917 1116
Size: 160 757 199
Fragments arranged by size:
757 199 160
BsmI GAATG_Cn'
Cuts at: 0 905 1116
Size: 905 211
BsmBI CGTCTCn'nnnn_
Cuts at: 0 951 1116
Size: 951 165
Bsp1286I G_dGCh'C
Cuts at: 0 142 160 263 751 873 917 968 1116
Size: 142 18 103 488 122 44 51 148
Fragments arranged by size:
488 148 142 122 103 51 44 18
BspEI T'CCGG_A
Cuts at: 0 781 1116
Size: 781 335
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 290 1116
Size: 290 826
BsrBI CCG'CTC
Cuts at: 0 32 223 1116
Size: 32 191 893
Fragments arranged by size:
893 191 32
BsrFI r'CCGG_y
Cuts at: 0 232 1116
Size: 232 884
BssHII G'CGCG_C
Cuts at: 0 251 1116
Size: 251 865
BstXI CCAn_nnnn'nTGG
Cuts at: 0 606 1116
Size: 606 510
BstYI r'GATC_y
Cuts at: 0 755 992 1116
Size: 755 237 124
Fragments arranged by size:
755 237 124
BtgI C'CryG_G
Cuts at: 0 48 641 1116
Size: 48 593 475
Fragments arranged by size:
593 475 48
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 255 398 1116
Size: 255 143 718
Fragments arranged by size:
718 255 143
BtsI GCAGTG_nn'
Cuts at: 0 582 980 1116
Size: 582 398 136
Fragments arranged by size:
582 398 136
DrdI GACnn_nn'nnGTC
Cuts at: 0 671 1116
Size: 671 445
EaeI y'GGCC_r
Cuts at: 0 89 131 1116
Size: 89 42 985
Fragments arranged by size:
985 89 42
EarI CTCTTCn'nnn_
Cuts at: 0 70 517 530 559 1116
Size: 70 447 13 29 557
Fragments arranged by size:
557 447 70 29 13
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 251 561 1116
Size: 251 310 555
Fragments arranged by size:
555 310 251
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 144 637 1043 1116
Size: 144 493 406 73
Fragments arranged by size:
493 406 144 73
EcoO109I rG'GnC_Cy
Cuts at: 0 259 612 773 1116
Size: 259 353 161 343
Fragments arranged by size:
353 343 259 161
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 466 651 1116
Size: 466 185 465
Fragments arranged by size:
466 465 185
HaeII r_GCGC'y
Cuts at: 0 38 325 1116
Size: 38 287 791
Fragments arranged by size:
791 287 38
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 298 330 487 519 700 732 1116
Size: 298 32 157 32 181 32 384
Fragments arranged by size:
384 298 181 157 32 32 32
HindIII A'AGCT_T
Cuts at: 0 619 1116
Size: 619 497
KasI G'GCGC_C
Cuts at: 0 34 1116
Size: 34 1082
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 816 1116
Size: 816 300
MslI CAynn'nnrTG
Cuts at: 0 506 1116
Size: 506 610
MspA1I CmG'CkG
Cuts at: 0 50 408 1116
Size: 50 358 708
Fragments arranged by size:
708 358 50
NaeI GCC'GGC
Cuts at: 0 234 1116
Size: 234 882
NarI GG'CG_CC
Cuts at: 0 35 1116
Size: 35 1081
NgoMIV G'CCGG_C
Cuts at: 0 232 1116
Size: 232 884
NspI r_CATG'y
Cuts at: 0 313 501 1116
Size: 313 188 615
Fragments arranged by size:
615 313 188
PfoI T'CCnGG_A
Cuts at: 0 244 1116
Size: 244 872
PpuMI rG'GwC_Cy
Cuts at: 0 612 773 1116
Size: 612 161 343
Fragments arranged by size:
612 343 161
PspOMI G'GGCC_C
Cuts at: 0 259 1116
Size: 259 857
PstI C_TGCA'G
Cuts at: 0 202 1116
Size: 202 914
PvuII CAG'CTG
Cuts at: 0 408 1116
Size: 408 708
RsrII CG'GwC_CG
Cuts at: 0 10 1116
Size: 10 1106
SacII CC_GC'GG
Cuts at: 0 51 1116
Size: 51 1065
SapI GCTCTTCn'nnn_
Cuts at: 0 70 559 1116
Size: 70 489 557
Fragments arranged by size:
557 489 70
SfcI C'TryA_G
Cuts at: 0 198 1116
Size: 198 918
SfoI GGC'GCC
Cuts at: 0 36 1116
Size: 36 1080
SmaI CCC'GGG
Cuts at: 0 319 1032 1116
Size: 319 713 84
Fragments arranged by size:
713 319 84
SmlI C'TyrA_G
Cuts at: 0 954 1116
Size: 954 162
StuI AGG'CCT
Cuts at: 0 396 466 1116
Size: 396 70 650
Fragments arranged by size:
650 396 70
StyI C'CwwG_G
Cuts at: 0 450 461 1116
Size: 450 11 655
Fragments arranged by size:
655 450 11
XbaI T'CTAG_A
Cuts at: 0 675 1116
Size: 675 441
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 99 1116
Size: 99 1017
XmaI C'CCGG_G
Cuts at: 0 317 1030 1116
Size: 317 713 86
Fragments arranged by size:
713 317 86
Enzymes that do cut:
AcuI ApaI ApoI AvaI BanI BanII BbeI BbsI
BbvCI BglI Bme1580I BmrI BpmI Bpu10I BpuEI BsaAI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiHKAI BsmI
BsmBI Bsp1286I BspEI BspMI BsrBI BsrFI BssHII BstXI
BstYI BtgI BtgZI BtsI DrdI EaeI EarI EciI
Eco57MI EcoO109I FalI HaeII Hin4I HindIII KasI MmeI
MslI MspA1I NaeI NarI NgoMIV NspI PfoI PpuMI
PspOMI PstI PvuII RsrII SacII SapI SfcI SfoI
SmaI SmlI StuI StyI XbaI XcmI XmaI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AfeI AflII AflIII
AgeI AhdI AleI AlfI AloI AlwNI ApaLI AscI
AseI AsiSI AvrII BaeI BamHI BcgI BciVI BclI
BfrBI BglII BlpI BmgBI BmtI BplI BsaI BsaBI
BsiEI BsiWI BspHI BsrDI BsrGI BssSI BstAPI BstBI
BstEII BstZ17I Bsu36I ClaI CspCI DraI DraIII EagI
EcoICRI EcoNI EcoRI EcoRV FseI FspI FspAI HincII
HpaI KpnI MfeI MluI MscI NcoI NdeI NheI
NotI NruI NsiI PacI PasI PciI PflMI PmeI
PmlI PpiI PshAI PsiI PspXI PsrI PvuI SacI
SalI SanDI SbfI ScaI SexAI SfiI SgfI SgrAI
SnaBI SpeI SphI SrfI SspI SwaI TaqII TatI
TstI Tth111I XhoI XmnI ZraI