(Circular) (Six-base) MAPSORT of: pFN21AB5655.seq Check: 2516 from: 1 to: 8389 pFN21AB5655 8389 bp With 182 enzymes: SgfI * January 16, 2009 15:25 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2284 2284 Size: 8389 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 6199 278 Size: 53 83 186 936 4663 2468 Fragments arranged by size: 4663 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3644 5732 1937 Size: 1707 2088 4594 Fragments arranged by size: 4594 2088 1707 Acc65I G'GTAC_C Cuts at: 5710 5710 Size: 8389 AclI AA'CG_TT Cuts at: 1563 6516 6889 1563 Size: 4953 373 3063 Fragments arranged by size: 4953 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2115 2247 2414 3891 4865 6452 7836 8104 Size: 483 132 167 1477 974 1587 1384 268 Cuts at: 8104 1632 Size: 1917 Fragments arranged by size: 1917 1587 1477 1384 974 483 268 167 132 AflII C'TTAA_G Cuts at: 820 1017 3824 5045 820 Size: 197 2807 1221 4164 Fragments arranged by size: 4164 2807 1221 197 AflIII A'CryG_T Cuts at: 1203 3422 3639 3786 3847 7288 8265 1203 Size: 2219 217 147 61 3441 977 1327 Fragments arranged by size: 3441 2219 1327 977 217 147 61 AgeI A'CCGG_T Cuts at: 2726 7220 2726 Size: 4494 3895 AhdI GACnn_n'nnGTC Cuts at: 4600 7118 4600 Size: 2518 5871 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 8389 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 8389 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3056 3088 3056 Size: 32 8357 AlwNI CAG_nnn'CTG Cuts at: 3608 6132 7704 3608 Size: 2524 1572 4293 Fragments arranged by size: 4293 2524 1572 ApaLI G'TGCA_C Cuts at: 2271 6446 7602 2271 Size: 4175 1156 3058 Fragments arranged by size: 4175 3058 1156 ApoI r'AATT_y Cuts at: 1493 2423 2605 3113 3192 3815 3841 4069 4573 Size: 930 182 508 79 623 26 228 504 Cuts at: 4573 4702 4898 5411 5418 5698 5924 6076 8185 Size: 129 196 513 7 280 226 152 2109 Cuts at: 8185 1493 Size: 1697 Fragments arranged by size: 2109 1697 930 623 513 508 504 280 228 226 196 182 152 129 79 26 7 AseI AT'TA_AT Cuts at: 160 1032 3153 6943 160 Size: 872 2121 3790 1606 Fragments arranged by size: 3790 2121 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 8389 AvaI C'yCGr_G Cuts at: 1870 1943 2178 5714 1870 Size: 73 235 3536 4545 Fragments arranged by size: 4545 3536 235 73 BamHI G'GATC_C Cuts at: 2732 5719 6086 2732 Size: 2987 367 5035 Fragments arranged by size: 5035 2987 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 5710 7165 618 Size: 325 217 627 3923 1455 1842 Fragments arranged by size: 3923 1842 1455 627 325 217 BanII G_rGCy'C Cuts at: 1391 2539 2857 5708 1391 Size: 1148 318 2851 4072 Fragments arranged by size: 4072 2851 1148 318 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2120 2228 2235 3789 928 Size: 971 221 108 7 1554 5528 Fragments arranged by size: 5528 1554 971 221 108 7 BbvCI CC'TCA_GC Cuts at: 2280 2555 2280 Size: 275 8114 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3332 3366 6580 6614 1903 Size: 34 1395 34 3214 34 3678 Fragments arranged by size: 3678 3214 1395 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2819 6281 7491 2819 Size: 3462 1210 3717 Fragments arranged by size: 3717 3462 1210 BclI T'GATC_A Cuts at: 1548 2980 1548 Size: 1432 6957 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 7000 136 Size: 107 122 71 631 605 5328 1525 Fragments arranged by size: 5328 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 2747 8384 2747 Size: 5637 2752 BlpI GC'TnA_GC Cuts at: 2925 5806 2925 Size: 2881 5508 Bme1580I G_kGCm'C Cuts at: 1790 2275 5168 6450 7606 1790 Size: 485 2893 1282 1156 2573 Fragments arranged by size: 2893 2573 1282 1156 485 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 8389 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 5577 6120 7078 Size: 416 53 83 399 24 4160 543 958 Cuts at: 7078 442 Size: 1753 Fragments arranged by size: 4160 1753 958 543 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 2370 1056 Size: 1314 7075 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3995 3995 Size: 8389 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2193 2406 2511 2538 3585 5250 7049 1239 Size: 954 213 105 27 1047 1665 1799 2579 Fragments arranged by size: 2579 1799 1665 1047 954 213 105 27 Bpu10I CC'TnA_GC Cuts at: 2280 2555 2280 Size: 275 8114 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3478 3983 4914 6514 7379 7677 3478 Size: 505 931 1600 865 298 4190 Fragments arranged by size: 4190 1600 931 865 505 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 7052 882 Size: 617 5553 2219 Fragments arranged by size: 5553 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 5169 5328 6180 493 Size: 711 3965 159 852 2702 Fragments arranged by size: 3965 2702 852 711 159 BsaBI GATnn'nnATC Cuts at: 8383 8383 Size: 8389 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 6196 6578 7213 275 Size: 53 83 186 936 4663 382 635 1451 Fragments arranged by size: 4663 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2726 6822 7220 7494 7641 8052 2726 Size: 4096 398 274 147 411 3063 Fragments arranged by size: 4096 3063 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2239 2269 2803 2833 3056 Size: 30 165 30 836 30 534 30 223 Cuts at: 3056 3086 3746 3776 1178 Size: 30 660 30 5791 Fragments arranged by size: 5791 836 660 534 223 165 30 30 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2931 3064 5138 1186 Size: 1745 133 2074 4437 Fragments arranged by size: 4437 2074 1745 133 BseYI C'CCAG_C Cuts at: 3321 7592 8088 3321 Size: 4271 496 3622 Fragments arranged by size: 4271 3622 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 5215 1163 Size: 573 3479 4337 Fragments arranged by size: 4337 3479 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 4970 5839 6600 7628 1540 Size: 275 186 2969 869 761 1028 2301 Fragments arranged by size: 2969 2301 1028 869 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2275 3478 5473 6450 6535 7606 2275 Size: 1203 1995 977 85 1071 3058 Fragments arranged by size: 3058 1995 1203 1071 977 85 BsmI GAATG_Cn' Cuts at: 2077 3501 3601 4834 5900 5993 2077 Size: 1424 100 1233 1066 93 4473 Fragments arranged by size: 4473 1424 1233 1066 100 93 BsmBI CGTCTCn'nnnn_ Cuts at: 3745 3745 Size: 8389 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2275 2539 2857 3478 5168 5473 5708 Size: 399 485 264 318 621 1690 305 235 Cuts at: 5708 6450 6535 7606 1391 Size: 742 85 1071 2174 Fragments arranged by size: 2174 1690 1071 742 621 485 399 318 305 264 235 85 BspEI T'CCGG_A Cuts at: 8052 8052 Size: 8389 BspHI T'CATG_A Cuts at: 6278 8008 6278 Size: 1730 6659 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2284 4415 5744 844 Size: 1440 2131 1329 3489 Fragments arranged by size: 3489 2131 1440 1329 BsrBI CCG'CTC Cuts at: 1394 1823 6276 1394 Size: 429 4453 3507 Fragments arranged by size: 4453 3507 429 BsrDI GCAATG_nn' Cuts at: 65 1248 3006 5021 6884 7058 65 Size: 1183 1758 2015 1863 174 1396 Fragments arranged by size: 2015 1863 1758 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2726 7033 7220 1700 Size: 110 916 4307 187 2869 Fragments arranged by size: 4307 2869 916 187 110 BsrGI T'GTAC_A Cuts at: 96 2644 96 Size: 2548 5841 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 8389 BssSI C'ACGA_G Cuts at: 6449 7461 6449 Size: 1012 7377 BstAPI GCAn_nnn'nTGC Cuts at: 794 2167 5315 794 Size: 1373 3148 3868 Fragments arranged by size: 3868 3148 1373 BstBI TT'CG_AA Cuts at: 2610 7193 2610 Size: 4583 3806 BstEII G'GTnAC_C Cuts at: 1184 2951 1184 Size: 1767 6622 BstYI r'GATC_y Cuts at: 1972 2732 2747 3945 4284 5282 5719 6086 6471 Size: 760 15 1198 339 998 437 367 385 Cuts at: 6471 6488 7940 8026 8038 8384 1972 Size: 17 1452 86 12 346 1977 Fragments arranged by size: 1977 1452 1198 998 760 437 385 367 346 339 86 17 15 12 Bsu36I CC'TnA_GG Cuts at: 2094 5121 2094 Size: 3027 5362 BtgI C'CryG_G Cuts at: 513 1065 2540 5301 513 Size: 552 1475 2761 3601 Fragments arranged by size: 3601 2761 1475 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 7650 Fragments arranged by size: 7650 683 56 BtsI GCAGTG_nn' Cuts at: 2249 4660 5905 6699 6719 2249 Size: 2411 1245 794 20 3919 Fragments arranged by size: 3919 2411 1245 794 20 ClaI AT'CG_AT Cuts at: 1554 4966 1554 Size: 3412 4977 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 8354 DraI TTT'AAA Cuts at: 3190 3814 5693 3190 Size: 624 1879 5886 Fragments arranged by size: 5886 1879 624 DraIII CAC_nnn'GTG Cuts at: 3394 3394 Size: 8389 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 2684 7396 809 Size: 507 1368 4712 1802 Fragments arranged by size: 4712 1802 1368 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 5836 6725 8354 Size: 54 1425 50 275 18 4006 889 1629 Cuts at: 8354 8 Size: 43 Fragments arranged by size: 4006 1629 1425 889 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 5836 1537 Size: 275 4024 4090 Fragments arranged by size: 4090 4024 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3018 3129 3236 3831 4689 6319 1353 Size: 1665 111 107 595 858 1630 3423 Fragments arranged by size: 3423 1665 1630 858 595 111 107 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2207 6976 7350 7496 387 Size: 1064 138 618 4769 374 146 1280 Fragments arranged by size: 4769 1280 1064 618 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2115 2193 2247 2406 2414 2511 2538 Size: 393 483 78 54 159 8 97 27 Cuts at: 2538 3585 3891 4865 5250 6452 7049 7836 8104 Size: 1047 306 974 385 1202 597 787 268 Cuts at: 8104 1239 Size: 1524 Fragments arranged by size: 1524 1202 1047 974 787 597 483 393 385 306 268 159 97 78 54 27 8 EcoO109I rG'GnC_Cy Cuts at: 2711 3259 2711 Size: 548 7841 EcoRI G'AATT_C Cuts at: 5418 5698 6076 5418 Size: 280 378 7731 Fragments arranged by size: 7731 378 280 EcoRV GAT'ATC Cuts at: 2760 2760 Size: 8389 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2252 3084 2252 Size: 832 7557 FspI TGC'GCA Cuts at: 6895 6895 Size: 8389 HaeII r_GCGC'y Cuts at: 1678 7536 1678 Size: 5858 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2010 2042 2090 2122 2749 Size: 32 135 32 438 32 48 32 627 Cuts at: 2749 2781 2803 2835 3626 3658 3715 3746 3747 Size: 32 22 32 791 32 57 31 1 Cuts at: 3747 3776 3778 3808 4027 4059 4586 4618 7030 Size: 29 2 30 219 32 527 32 2412 Cuts at: 7030 7062 7104 7136 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 2412 791 627 527 438 219 135 57 48 42 32 32 32 32 32 32 32 32 32 32 32 31 30 29 22 2 1 HincII GTy'rAC Cuts at: 669 1657 1938 2846 5733 5979 669 Size: 988 281 908 2887 246 3079 Fragments arranged by size: 3079 2887 988 908 281 246 HindIII A'AGCT_T Cuts at: 748 5240 5510 748 Size: 4492 270 3627 Fragments arranged by size: 4492 3627 270 HpaI GTT'AAC Cuts at: 5979 5979 Size: 8389 KpnI G_GTAC'C Cuts at: 5714 5714 Size: 8389 MfeI C'AATT_G Cuts at: 5988 5988 Size: 8389 MluI A'CGCG_T Cuts at: 8265 8265 Size: 8389 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2461 2488 4482 4701 7503 7687 1305 Size: 1156 27 1994 219 2802 184 2007 Fragments arranged by size: 2802 2007 1994 1156 219 184 27 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 6567 Fragments arranged by size: 6567 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 5328 6347 6706 6865 518 Size: 648 4162 1019 359 159 2042 Fragments arranged by size: 4162 2042 1019 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2906 3605 5805 6482 7630 7875 1606 Size: 1300 699 2200 677 1148 245 2120 Fragments arranged by size: 2200 2120 1300 1148 699 677 245 NcoI C'CATG_G Cuts at: 513 1065 5301 513 Size: 552 4236 3601 Fragments arranged by size: 4236 3601 552 NdeI CA'TA_TG Cuts at: 387 3997 387 Size: 3610 4779 NheI G'CTAG_C Cuts at: 1052 2366 1052 Size: 1314 7075 NotI GC'GGCC_GC Cuts at: 5836 5836 Size: 8389 NspI r_CATG'y Cuts at: 1132 1229 3211 3426 3643 3790 3851 5747 7292 Size: 97 1982 215 217 147 61 1896 1545 Cuts at: 7292 1132 Size: 2229 Fragments arranged by size: 2229 1982 1896 1545 217 215 147 97 61 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 8389 PciI A'CATG_T Cuts at: 3422 3639 3786 3847 7288 3422 Size: 217 147 61 3441 4523 Fragments arranged by size: 4523 3441 217 147 61 PflMI CCAn_nnn'nTGG Cuts at: 1108 5405 1108 Size: 4297 4092 PfoI T'CCnGG_A Cuts at: 2383 2821 2383 Size: 438 7951 PmeI GTTT'AAAC Cuts at: 5693 5693 Size: 8389 PmlI CAC'GTG Cuts at: 5169 5169 Size: 8389 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3056 3088 6458 6490 7971 8003 3056 Size: 32 3370 32 1481 32 3442 Fragments arranged by size: 3442 3370 1481 32 32 32 PpuMI rG'GwC_Cy Cuts at: 2711 3259 2711 Size: 548 7841 PshAI GACnn'nnGTC Cuts at: 1613 4370 1613 Size: 2757 5632 PsiI TTA'TAA Cuts at: 4208 5959 4208 Size: 1751 6638 PsrI GAACnnnnnnTACnnnnnnn_nnnnn' Cuts at: 4466 4498 5176 5208 4466 Size: 32 678 32 7647 Fragments arranged by size: 7647 678 32 32 PstI C_TGCA'G Cuts at: 830 3021 5207 5741 830 Size: 2191 2186 534 3478 Fragments arranged by size: 3478 2191 2186 534 PvuI CG_AT'CG Cuts at: 2001 4970 2001 Size: 2969 5420 PvuII CAG'CTG Cuts at: 2906 3605 2906 Size: 699 7690 SalI G'TCGA_C Cuts at: 1936 5731 1936 Size: 3795 4594 SapI GCTCTTCn'nnn_ Cuts at: 3236 4689 3236 Size: 1453 6936 SbfI CC_TGCA'GG Cuts at: 5741 5741 Size: 8389 ScaI AGT'ACT Cuts at: 4264 6637 8349 4264 Size: 2373 1712 4304 Fragments arranged by size: 4304 2373 1712 SexAI A'CCwGG_T Cuts at: 2651 2682 5344 2651 Size: 31 2662 5696 Fragments arranged by size: 5696 2662 31 SfcI C'TryA_G Cuts at: 826 1045 2773 3017 3219 5137 5203 5737 6872 Size: 219 1728 244 202 1918 66 534 1135 Cuts at: 6872 7553 7744 826 Size: 681 191 1471 Fragments arranged by size: 1918 1728 1471 1135 681 534 244 219 202 191 66 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 8389 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 8389 SmaI CCC'GGG Cuts at: 1872 5716 1872 Size: 3844 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 3493 3824 3962 4929 5045 6493 Size: 197 926 1550 331 138 967 116 1448 Cuts at: 6493 7394 7656 820 Size: 901 262 1553 Fragments arranged by size: 1553 1550 1448 967 926 901 331 262 197 138 116 SnaBI TAC'GTA Cuts at: 493 5328 6180 493 Size: 4835 852 2702 Fragments arranged by size: 4835 2702 852 SpeI A'CTAG_T Cuts at: 152 4049 152 Size: 3897 4492 SphI G_CATG'C Cuts at: 1132 5747 1132 Size: 4615 3774 SspI AAT'ATT Cuts at: 5 52 3037 5533 6313 5 Size: 47 2985 2496 780 2081 Fragments arranged by size: 2985 2496 2081 780 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 8389 StyI C'CwwG_G Cuts at: 513 1065 5301 5828 513 Size: 552 4236 527 3074 Fragments arranged by size: 4236 3074 552 527 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 5314 6416 6433 6586 6771 7216 325 Size: 1255 3734 1102 17 153 185 445 1498 Fragments arranged by size: 3734 1498 1255 1102 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2644 3157 3550 Size: 274 80 33 51 1443 667 513 393 Cuts at: 3550 3636 4120 4262 5448 6635 8347 96 Size: 86 484 142 1186 1187 1712 138 Fragments arranged by size: 1712 1443 1187 1186 667 513 484 393 274 142 138 86 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2798 2830 5475 5507 8349 8381 2798 Size: 32 2645 32 2842 32 2806 Fragments arranged by size: 2842 2806 2645 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 8389 XbaI T'CTAG_A Cuts at: 3478 5725 3478 Size: 2247 6142 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2671 2671 Size: 8389 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 8389 XmaI C'CCGG_G Cuts at: 1870 5714 1870 Size: 3844 4545 XmnI GAAnn'nnTTC Cuts at: 6518 6518 Size: 8389 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 6197 276 Size: 53 83 186 936 4663 2468 Fragments arranged by size: 4663 2468 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI EcoRV FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PsrI PstI PvuI PvuII SalI SapI SbfI ScaI SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI ApaI AscI AvrII BaeI BbeI BfrBI BsiWI BstXI BstZ17I EcoICRI EcoNI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PspOMI PspXI RsrII SacI SacII SanDI SfiI SfoI SrfI SwaI