Restriction map for
pFN21AB5649
(Circular) (Six-base) MAPSORT of: pFN21AB5649.seq Check: 1890 from: 1 to: 6922
pFN21AB5649 6922 bp
With 182 enzymes: SgfI *
February 27, 2009 14:34 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4732 278
Size: 53 83 186 936 3196 2468
Fragments arranged by size:
3196 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2742 4265 1937
Size: 805 1523 4594
Fragments arranged by size:
4594 1523 805
Acc65I G'GTAC_C
Cuts at: 2468 4243 2468
Size: 1775 5147
AclI AA'CG_TT
Cuts at: 1563 2034 5049 5422 1563
Size: 471 3015 373 3063
Fragments arranged by size:
3063 3015 471 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 1995 2076 2869 3704 4985 6369 6637 1632
Size: 363 81 793 835 1281 1384 268 1917
Fragments arranged by size:
1917 1384 1281 835 793 363 268 81
AflII C'TTAA_G
Cuts at: 820 1017 2990 820
Size: 197 1973 4752
Fragments arranged by size:
4752 1973 197
AflIII A'CryG_T
Cuts at: 1203 2878 5821 6798 1203
Size: 1675 2943 977 1327
Fragments arranged by size:
2943 1675 1327 977
AgeI A'CCGG_T
Cuts at: 5753 5753
Size: 6922
AhdI GACnn_n'nnGTC
Cuts at: 5651 5651
Size: 6922
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6922
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6922
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 4029 4061 4176 4208 4029
Size: 32 115 32 6743
Fragments arranged by size:
6743 115 32 32
AlwNI CAG_nnn'CTG
Cuts at: 4048 4665 6237 4048
Size: 617 1572 4733
Fragments arranged by size:
4733 1572 617
ApaLI G'TGCA_C
Cuts at: 4979 6135 4979
Size: 1156 5766
ApoI r'AATT_y
Cuts at: 1493 3494 4231 4457 4609 6718 1493
Size: 2001 737 226 152 2109 1697
Fragments arranged by size:
2109 2001 1697 737 226 152
AseI AT'TA_AT
Cuts at: 160 1032 5476 160
Size: 872 4444 1606
Fragments arranged by size:
4444 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6922
AvaI C'yCGr_G
Cuts at: 1870 1943 4247 1870
Size: 73 2304 4545
Fragments arranged by size:
4545 2304 73
BamHI G'GATC_C
Cuts at: 2082 2163 3993 4252 4619 2082
Size: 81 1830 259 367 4385
Fragments arranged by size:
4385 1830 367 259 81
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2371 2468 2780 2995 4243
Size: 325 217 627 584 97 312 215 1248
Cuts at: 4243 5698 618
Size: 1455 1842
Fragments arranged by size:
1842 1455 1248 627 584 325 312 217
215 97
BanII G_rGCy'C
Cuts at: 1391 2829 2858 3487 4241 1391
Size: 1438 29 629 754 4072
Fragments arranged by size:
4072 1438 754 629 29
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2001 2875 928
Size: 971 102 874 4975
Fragments arranged by size:
4975 971 874 102
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 5113 5147 1903
Size: 34 3176 34 3678
Fragments arranged by size:
3678 3176 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2868 3529 4814 6024 2868
Size: 661 1285 1210 3766
Fragments arranged by size:
3766 1285 1210 661
BclI T'GATC_A
Cuts at: 1548 2140 3459 1548
Size: 592 1319 5011
Fragments arranged by size:
5011 1319 592
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 5533 136
Size: 107 122 71 631 605 3861 1525
Fragments arranged by size:
3861 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 6917 6917
Size: 6922
BlpI GC'TnA_GC
Cuts at: 4339 4339
Size: 6922
Bme1580I G_kGCm'C
Cuts at: 1790 2376 4983 6139 1790
Size: 586 2607 1156 2573
Fragments arranged by size:
2607 2573 1156 586
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6922
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2371 3111 4653
Size: 416 53 83 399 24 954 740 1542
Cuts at: 4653 5611 442
Size: 958 1753
Fragments arranged by size:
1753 1542 958 954 740 416 399 83
53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6922
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2712 5582 1239
Size: 1473 2870 2579
Fragments arranged by size:
2870 2579 1473
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 5047 5912 6210 5047
Size: 865 298 5759
Fragments arranged by size:
5759 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2126 5585 882
Size: 617 627 3459 2219
Fragments arranged by size:
3459 2219 627 617
BsaAI yAC'GTr
Cuts at: 493 1204 4713 493
Size: 711 3509 2702
Fragments arranged by size:
3509 2702 711
BsaBI GATnn'nnATC
Cuts at: 6916 6916
Size: 6922
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 4729 5111 5746 275
Size: 53 83 186 936 3196 382 635 1451
Fragments arranged by size:
3196 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 5355 5753 6027 6174 6585 5355
Size: 398 274 147 411 5692
Fragments arranged by size:
5692 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 3511 3541 4176 4206 1178
Size: 30 165 30 2108 30 635 30 3894
Fragments arranged by size:
3894 2108 635 165 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 6922
BseYI C'CCAG_C
Cuts at: 6125 6621 6125
Size: 496 6426
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2263 3336 3555 1163
Size: 573 527 1073 219 4530
Fragments arranged by size:
4530 1073 573 527 219
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 4372 5133 6161 1540
Size: 275 186 2371 761 1028 2301
Fragments arranged by size:
2371 2301 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2578 2609 4983 5068 6139 2578
Size: 31 2374 85 1071 3361
Fragments arranged by size:
3361 2374 1071 85 31
BsmI GAATG_Cn'
Cuts at: 3788 4433 4526 3788
Size: 645 93 6184
Fragments arranged by size:
6184 645 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2376 2578 2609 2829 2858 3487 4241
Size: 399 586 202 31 220 29 629 754
Cuts at: 4241 4983 5068 6139 1391
Size: 742 85 1071 2174
Fragments arranged by size:
2174 1071 754 742 629 586 399 220
202 85 31 29
BspEI T'CCGG_A
Cuts at: 6585 6585
Size: 6922
BspHI T'CATG_A
Cuts at: 4811 6541 4811
Size: 1730 5192
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 4277 844
Size: 3433 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 4809 1394
Size: 429 2986 3507
Fragments arranged by size:
3507 2986 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2382 5417 5591 65
Size: 1183 1134 3035 174 1396
Fragments arranged by size:
3035 1396 1183 1134 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 5566 5753 1700
Size: 110 3756 187 2869
Fragments arranged by size:
3756 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 2427 96
Size: 2331 4591
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6922
BssSI C'ACGA_G
Cuts at: 4982 5994 4982
Size: 1012 5910
BstAPI GCAn_nnn'nTGC
Cuts at: 794 3323 794
Size: 2529 4393
BstBI TT'CG_AA
Cuts at: 5726 5726
Size: 6922
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6922
BstYI r'GATC_y
Cuts at: 1972 2082 2163 3502 3993 4252 4619 5004 5021
Size: 110 81 1339 491 259 367 385 17
Cuts at: 5021 6473 6559 6571 6917 1972
Size: 1452 86 12 346 1977
Fragments arranged by size:
1977 1452 1339 491 385 367 346 259
110 86 81 17 12
Bsu36I CC'TnA_GG
Cuts at: 2584 2584
Size: 6922
BtgI C'CryG_G
Cuts at: 513 1065 513
Size: 552 6370
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2723 4163 487
Size: 683 56 1497 1440 3246
Fragments arranged by size:
3246 1497 1440 683 56
BtsI GCAGTG_nn'
Cuts at: 2300 3572 3659 3970 4438 5232 5252 2300
Size: 1272 87 311 468 794 20 3970
Fragments arranged by size:
3970 1272 794 468 311 87 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6922
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6887
DraI TTT'AAA
Cuts at: 3790 4226 3790
Size: 436 6486
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5929 809
Size: 507 4613 1802
Fragments arranged by size:
4613 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2404 4369 5258
Size: 54 1425 50 275 18 574 1965 889
Cuts at: 5258 6887 8
Size: 1629 43
Fragments arranged by size:
1965 1629 1425 889 574 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 4369 1537
Size: 275 2557 4090
Fragments arranged by size:
4090 2557 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3672 4133 4852 1353
Size: 2319 461 719 3423
Fragments arranged by size:
3423 2319 719 461
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 5509 5883 6029 387
Size: 1064 138 3920 374 146 1280
Fragments arranged by size:
3920 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 1995 2076 2712 2869 3704 4985 5582
Size: 393 363 81 636 157 835 1281 597
Cuts at: 5582 6369 6637 1239
Size: 787 268 1524
Fragments arranged by size:
1524 1281 835 787 636 597 393 363
268 157 81
EcoNI CCTnn'n_nnAGG
Cuts at: 2583 2583
Size: 6922
EcoRI G'AATT_C
Cuts at: 4231 4609 4231
Size: 378 6544
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 4139 4139
Size: 6922
FspI TGC'GCA
Cuts at: 5428 5428
Size: 6922
HaeII r_GCGC'y
Cuts at: 1678 6069 1678
Size: 4391 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2125 2157 2327 2359 3893
Size: 32 135 32 553 32 170 32 1534
Cuts at: 3893 3925 5563 5595 5637 5669 1373
Size: 32 1638 32 42 32 2626
Fragments arranged by size:
2626 1638 1534 553 170 135 42 32
32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2533 3817 4266 4512 669
Size: 988 281 595 1284 449 246 3079
Fragments arranged by size:
3079 1284 988 595 449 281 246
HindIII A'AGCT_T
Cuts at: 748 2987 748
Size: 2239 4683
HpaI GTT'AAC
Cuts at: 4512 4512
Size: 6922
KpnI G_GTAC'C
Cuts at: 2472 4247 2472
Size: 1775 5147
MfeI C'AATT_G
Cuts at: 3465 4521 3465
Size: 1056 5866
MluI A'CGCG_T
Cuts at: 6798 6798
Size: 6922
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 6036 6220 1305
Size: 4731 184 2007
Fragments arranged by size:
4731 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2406 10
Size: 54 1425 343 574 4526
Fragments arranged by size:
4526 1425 574 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2486 4880 5239 5398 518
Size: 648 1320 2394 359 159 2042
Fragments arranged by size:
2394 2042 1320 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2054 4338 5015 6163 6408 1606
Size: 448 2284 677 1148 245 2120
Fragments arranged by size:
2284 2120 1148 677 448 245
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 6370
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6922
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6922
NotI GC'GGCC_GC
Cuts at: 4369 4369
Size: 6922
NspI r_CATG'y
Cuts at: 1132 1229 2882 4280 5825 1132
Size: 97 1653 1398 1545 2229
Fragments arranged by size:
2229 1653 1545 1398 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6922
PciI A'CATG_T
Cuts at: 2878 5821 2878
Size: 2943 3979
PflMI CCAn_nnn'nTGG
Cuts at: 1108 3516 1108
Size: 2408 4514
PfoI T'CCnGG_A
Cuts at: 3897 3897
Size: 6922
PmeI GTTT'AAAC
Cuts at: 4226 4226
Size: 6922
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4176 4208 4991 5023 6504 6536 4176
Size: 32 783 32 1481 32 4562
Fragments arranged by size:
4562 1481 783 32 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6922
PsiI TTA'TAA
Cuts at: 3072 4492 3072
Size: 1420 5502
PstI C_TGCA'G
Cuts at: 830 2229 3965 4274 830
Size: 1399 1736 309 3478
Fragments arranged by size:
3478 1736 1399 309
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6922
PvuII CAG'CTG
Cuts at: 2054 2054
Size: 6922
SalI G'TCGA_C
Cuts at: 1936 4264 1936
Size: 2328 4594
SbfI CC_TGCA'GG
Cuts at: 4274 4274
Size: 6922
ScaI AGT'ACT
Cuts at: 2113 2505 3583 5170 6882 2113
Size: 392 1078 1587 1712 2153
Fragments arranged by size:
2153 1712 1587 1078 392
SfcI C'TryA_G
Cuts at: 826 1045 2225 2543 3153 3948 3961 4132 4270
Size: 219 1180 318 610 795 13 171 138
Cuts at: 4270 5405 6086 6277 826
Size: 1135 681 191 1471
Fragments arranged by size:
1471 1180 1135 795 681 610 318 219
191 171 138 13
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6922
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6922
SmaI CCC'GGG
Cuts at: 1872 4249 1872
Size: 2377 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2990 5026 5927 6189 820
Size: 197 926 1047 2036 901 262 1553
Fragments arranged by size:
2036 1553 1047 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 4713 493
Size: 4220 2702
SpeI A'CTAG_T
Cuts at: 152 3311 152
Size: 3159 3763
SphI G_CATG'C
Cuts at: 1132 4280 1132
Size: 3148 3774
SspI AAT'ATT
Cuts at: 5 52 4100 4846 5
Size: 47 4048 746 2081
Fragments arranged by size:
4048 2081 746 47
StuI AGG'CCT
Cuts at: 1517 3594 1517
Size: 2077 4845
StyI C'CwwG_G
Cuts at: 513 1065 3451 3933 4361 513
Size: 552 2386 482 428 3074
Fragments arranged by size:
3074 2386 552 482 428
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2056 2550 3130 4949 4966 5119 5304
Size: 1255 476 494 580 1819 17 153 185
Cuts at: 5304 5749 325
Size: 445 1498
Fragments arranged by size:
1819 1498 1255 580 494 476 445 185
153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2111 2427 2503
Size: 274 80 33 51 1443 134 316 76
Cuts at: 2503 3581 5168 6880 96
Size: 1078 1587 1712 138
Fragments arranged by size:
1712 1587 1443 1078 316 274 138 134
80 76 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2833 2865 2966 2998 6882 6914 2833
Size: 32 101 32 3884 32 2841
Fragments arranged by size:
3884 2841 101 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6922
XbaI T'CTAG_A
Cuts at: 4258 4258
Size: 6922
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6922
XmaI C'CCGG_G
Cuts at: 1870 4247 1870
Size: 2377 4545
XmnI GAAnn'nnTTC
Cuts at: 2593 4122 5051 2593
Size: 1529 929 4464
Fragments arranged by size:
4464 1529 929
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4730 276
Size: 53 83 186 936 3196 2468
Fragments arranged by size:
3196 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AloI AlwNI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI
BstAPI BstBI BstEII BstYI Bsu36I BtgI BtgZI BtsI
ClaI CspCI DraI DrdI EaeI EagI EarI EciI
Eco57MI EcoNI EcoRI FalI FspI HaeII Hin4I HincII
HindIII HpaI KpnI MfeI MluI MmeI MscI MslI
MspA1I NcoI NdeI NheI NotI NspI PasI PciI
PflMI PfoI PmeI PpiI PshAI PsiI PstI PvuI
PvuII SalI SbfI ScaI SfcI SgfI SgrAI SmaI
SmlI SnaBI SpeI SphI SspI StuI StyI TaqII
TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI ApaI AscI AvrII BaeI BbeI BbvCI
BfrBI BplI Bpu10I BsiWI BsmBI BstXI BstZ17I DraIII
EcoICRI EcoO109I EcoRV FseI FspAI KasI NaeI NarI
NgoMIV NruI NsiI PacI PmlI PpuMI PspOMI PspXI
PsrI RsrII SacI SacII SanDI SapI SexAI SfiI
SfoI SrfI SwaI XcmI