(Circular) (Six-base) MAPSORT of: pFN21AB5648.seq Check: 3774 from: 1 to: 7183 pFN21AB5648 7183 bp With 182 enzymes: SgfI * October 20, 2009 10:41 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4993 278 Size: 53 83 186 936 3457 2468 Fragments arranged by size: 3457 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2737 4345 4526 1937 Size: 800 1608 181 4594 Fragments arranged by size: 4594 1608 800 181 Acc65I G'GTAC_C Cuts at: 4504 4504 Size: 7183 AclI AA'CG_TT Cuts at: 1563 4482 5310 5683 1563 Size: 2919 828 373 3063 Fragments arranged by size: 3063 2919 828 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2127 3087 3648 5246 6630 6898 1632 Size: 495 960 561 1598 1384 268 1917 Fragments arranged by size: 1917 1598 1384 960 561 495 268 AflII C'TTAA_G Cuts at: 820 1017 3173 820 Size: 197 2156 4830 Fragments arranged by size: 4830 2156 197 AflIII A'CryG_T Cuts at: 1203 4071 4264 6082 7059 1203 Size: 2868 193 1818 977 1327 Fragments arranged by size: 2868 1818 1327 977 193 AgeI A'CCGG_T Cuts at: 3091 6014 3091 Size: 2923 4260 AhdI GACnn_n'nnGTC Cuts at: 3501 5912 3501 Size: 2411 4772 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 7183 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 7183 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 4090 4122 4090 Size: 32 7151 AlwNI CAG_nnn'CTG Cuts at: 3215 3263 4109 4926 6498 3215 Size: 48 846 817 1572 3900 Fragments arranged by size: 3900 1572 846 817 48 ApaLI G'TGCA_C Cuts at: 5240 6396 5240 Size: 1156 6027 ApoI r'AATT_y Cuts at: 1493 2706 2756 2989 3343 3593 4492 4718 4870 Size: 1213 50 233 354 250 899 226 152 Cuts at: 4870 6979 1493 Size: 2109 1697 Fragments arranged by size: 2109 1697 1213 899 354 250 233 226 152 50 AseI AT'TA_AT Cuts at: 160 1032 5737 160 Size: 872 4705 1606 Fragments arranged by size: 4705 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 7183 AvaI C'yCGr_G Cuts at: 1870 1943 2070 4508 1870 Size: 73 127 2438 4545 Fragments arranged by size: 4545 2438 127 73 BamHI G'GATC_C Cuts at: 4513 4880 4513 Size: 367 6816 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2012 4504 5959 618 Size: 325 217 627 225 2492 1455 1842 Fragments arranged by size: 2492 1842 1455 627 325 225 217 BanII G_rGCy'C Cuts at: 1391 4502 1391 Size: 3111 4072 BbeI G_GCGC'C Cuts at: 2016 2016 Size: 7183 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 3636 3909 928 Size: 971 1737 273 4202 Fragments arranged by size: 4202 1737 971 273 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 5374 5408 1903 Size: 34 3437 34 3678 Fragments arranged by size: 3678 3437 34 34 BciVI GTATCCnnnnn_n' Cuts at: 5075 6285 5075 Size: 1210 5973 BclI T'GATC_A Cuts at: 1548 2857 1548 Size: 1309 5874 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 5794 136 Size: 107 122 71 631 605 4122 1525 Fragments arranged by size: 4122 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 2084 7178 2084 Size: 5094 2089 BlpI GC'TnA_GC Cuts at: 4600 4600 Size: 7183 Bme1580I G_kGCm'C Cuts at: 1790 5244 6400 1790 Size: 3454 1156 2573 Fragments arranged by size: 3454 2573 1156 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 7183 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3546 4914 5872 Size: 416 53 83 399 24 2129 1368 958 Cuts at: 5872 442 Size: 1753 Fragments arranged by size: 2129 1753 1368 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 7183 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 3237 5843 1239 Size: 1998 2606 2579 Fragments arranged by size: 2606 2579 1998 Bpu10I CC'TnA_GC Cuts at: 3537 3537 Size: 7183 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2946 3299 5308 6173 6471 2946 Size: 353 2009 865 298 3658 Fragments arranged by size: 3658 2009 865 353 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 5846 882 Size: 617 4347 2219 Fragments arranged by size: 4347 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 2602 4974 493 Size: 711 1398 2372 2702 Fragments arranged by size: 2702 2372 1398 711 BsaBI GATnn'nnATC Cuts at: 4279 7177 4279 Size: 2898 4285 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2013 4990 5372 6007 Size: 53 83 186 936 480 2977 382 635 Cuts at: 6007 275 Size: 1451 Fragments arranged by size: 2977 1451 936 635 480 382 186 83 53 BsaWI w'CCGG_w Cuts at: 3091 5616 6014 6288 6435 6846 3091 Size: 2525 398 274 147 411 3428 Fragments arranged by size: 3428 2525 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 6958 Fragments arranged by size: 6958 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 1186 Size: 7183 BseYI C'CCAG_C Cuts at: 3208 4351 6386 6882 3208 Size: 1143 2035 496 3509 Fragments arranged by size: 3509 2035 1143 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2569 3393 1163 Size: 573 833 824 4953 Fragments arranged by size: 4953 833 824 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 4633 5394 6422 1540 Size: 275 186 2632 761 1028 2301 Fragments arranged by size: 2632 2301 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 5244 5329 6400 5244 Size: 85 1071 6027 Fragments arranged by size: 6027 1071 85 BsmI GAATG_Cn' Cuts at: 4153 4694 4787 4153 Size: 541 93 6549 Fragments arranged by size: 6549 541 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 4502 5244 5329 6400 1391 Size: 399 2712 742 85 1071 2174 Fragments arranged by size: 2712 2174 1071 742 399 85 BspEI T'CCGG_A Cuts at: 6846 6846 Size: 7183 BspHI T'CATG_A Cuts at: 2878 5072 6802 2878 Size: 2194 1730 3259 Fragments arranged by size: 3259 2194 1730 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2666 4538 844 Size: 1822 1872 3489 Fragments arranged by size: 3489 1872 1822 BsrBI CCG'CTC Cuts at: 1394 1823 2823 5070 1394 Size: 429 1000 2247 3507 Fragments arranged by size: 3507 2247 1000 429 BsrDI GCAATG_nn' Cuts at: 65 1248 3357 5678 5852 65 Size: 1183 2109 2321 174 1396 Fragments arranged by size: 2321 2109 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2015 3091 5827 6014 1700 Size: 110 205 1076 2736 187 2869 Fragments arranged by size: 2869 2736 1076 205 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 7183 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 7183 BssSI C'ACGA_G Cuts at: 5243 6255 5243 Size: 1012 6171 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 7183 BstBI TT'CG_AA Cuts at: 3391 5987 3391 Size: 2596 4587 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 7183 BstYI r'GATC_y Cuts at: 1972 2084 4513 4880 5265 5282 6734 6820 6832 Size: 112 2429 367 385 17 1452 86 12 Cuts at: 6832 7178 1972 Size: 346 1977 Fragments arranged by size: 2429 1977 1452 385 367 346 112 86 17 12 Bsu36I CC'TnA_GG Cuts at: 4110 4110 Size: 7183 BtgI C'CryG_G Cuts at: 513 1065 2004 513 Size: 552 939 5692 Fragments arranged by size: 5692 939 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 6444 Fragments arranged by size: 6444 683 56 BtsI GCAGTG_nn' Cuts at: 2581 3855 4699 5493 5513 2581 Size: 1274 844 794 20 4251 Fragments arranged by size: 4251 1274 844 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 7183 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 2920 2955 529 Size: 35 2356 35 4757 Fragments arranged by size: 4757 2356 35 35 DraI TTT'AAA Cuts at: 4487 4487 Size: 7183 DraIII CAC_nnn'GTG Cuts at: 2398 2398 Size: 7183 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 6190 809 Size: 507 4874 1802 Fragments arranged by size: 4874 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 4630 5519 7148 Size: 54 1425 50 275 18 2800 889 1629 Cuts at: 7148 8 Size: 43 Fragments arranged by size: 2800 1629 1425 889 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 4630 1537 Size: 275 2818 4090 Fragments arranged by size: 4090 2818 275 EarI CTCTTCn'nnn_ Cuts at: 1353 5113 1353 Size: 3760 3423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 5770 6144 6290 387 Size: 1064 138 4181 374 146 1280 Fragments arranged by size: 4181 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2127 3087 3237 3648 5246 5843 6630 Size: 393 495 960 150 411 1598 597 787 Cuts at: 6630 6898 1239 Size: 268 1524 Fragments arranged by size: 1598 1524 960 787 597 495 411 393 268 150 EcoNI CCTnn'n_nnAGG Cuts at: 4422 4442 4422 Size: 20 7163 EcoRI G'AATT_C Cuts at: 2989 3593 4492 4870 2989 Size: 604 899 378 5302 Fragments arranged by size: 5302 899 604 378 FspI TGC'GCA Cuts at: 5689 5689 Size: 7183 HaeII r_GCGC'y Cuts at: 1678 2016 6330 1678 Size: 338 4314 2531 Fragments arranged by size: 4314 2531 338 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2146 2159 2178 2191 2857 Size: 32 135 32 574 13 19 13 666 Cuts at: 2857 2889 3210 3242 3475 3487 3507 3519 3676 Size: 32 321 32 233 12 20 12 157 Cuts at: 3676 3708 3728 3760 3910 3942 5824 5856 5898 Size: 32 20 32 150 32 1882 32 42 Cuts at: 5898 5930 1373 Size: 32 2626 Fragments arranged by size: 2626 1882 666 574 321 233 157 150 135 42 32 32 32 32 32 32 32 32 32 20 20 19 13 13 12 12 HincII GTy'rAC Cuts at: 669 1657 1938 2738 4480 4527 4773 669 Size: 988 281 800 1742 47 246 3079 Fragments arranged by size: 3079 1742 988 800 281 246 47 HindIII A'AGCT_T Cuts at: 748 2500 748 Size: 1752 5431 HpaI GTT'AAC Cuts at: 4480 4773 4480 Size: 293 6890 KasI G'GCGC_C Cuts at: 2012 2012 Size: 7183 KpnI G_GTAC'C Cuts at: 4508 4508 Size: 7183 MfeI C'AATT_G Cuts at: 4782 4782 Size: 7183 MluI A'CGCG_T Cuts at: 7059 7059 Size: 7183 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 3582 3977 6297 6481 1305 Size: 2277 395 2320 184 2007 Fragments arranged by size: 2320 2277 2007 395 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 5361 Fragments arranged by size: 5361 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2418 2457 2604 5141 5500 5659 518 Size: 648 1252 39 147 2537 359 159 2042 Fragments arranged by size: 2537 2042 1252 648 359 159 147 39 MspA1I CmG'CkG Cuts at: 1606 3215 3371 4387 4599 5276 6424 6669 1606 Size: 1609 156 1016 212 677 1148 245 2120 Fragments arranged by size: 2120 1609 1148 1016 677 245 212 156 NaeI GCC'GGC Cuts at: 2017 2017 Size: 7183 NarI GG'CG_CC Cuts at: 2013 2013 Size: 7183 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 5692 Fragments arranged by size: 5692 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 7183 NgoMIV G'CCGG_C Cuts at: 2015 2015 Size: 7183 NheI G'CTAG_C Cuts at: 1052 1052 Size: 7183 NotI GC'GGCC_GC Cuts at: 4630 4630 Size: 7183 NspI r_CATG'y Cuts at: 1132 1229 4344 4541 6086 1132 Size: 97 3115 197 1545 2229 Fragments arranged by size: 3115 2229 1545 197 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 7183 PciI A'CATG_T Cuts at: 6082 6082 Size: 7183 PflMI CCAn_nnn'nTGG Cuts at: 1108 3215 1108 Size: 2107 5076 PmeI GTTT'AAAC Cuts at: 4487 4487 Size: 7183 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 5252 5284 6765 6797 5252 Size: 32 1481 32 5638 Fragments arranged by size: 5638 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 7183 PsiI TTA'TAA Cuts at: 4753 4753 Size: 7183 PstI C_TGCA'G Cuts at: 830 4446 4535 830 Size: 3616 89 3478 Fragments arranged by size: 3616 3478 89 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 7183 PvuII CAG'CTG Cuts at: 4387 4387 Size: 7183 SalI G'TCGA_C Cuts at: 1936 2736 4525 1936 Size: 800 1789 4594 Fragments arranged by size: 4594 1789 800 SbfI CC_TGCA'GG Cuts at: 4535 4535 Size: 7183 ScaI AGT'ACT Cuts at: 5431 7143 5431 Size: 1712 5471 SfcI C'TryA_G Cuts at: 826 1045 4101 4442 4531 5666 6347 6538 826 Size: 219 3056 341 89 1135 681 191 1471 Fragments arranged by size: 3056 1471 1135 681 341 219 191 89 SfoI GGC'GCC Cuts at: 2014 2014 Size: 7183 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 7183 SgrAI Cr'CCGG_yG Cuts at: 1700 2015 1700 Size: 315 6868 SmaI CCC'GGG Cuts at: 1872 4510 1872 Size: 2638 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2070 2961 3173 3278 5287 6188 Size: 197 926 127 891 212 105 2009 901 Cuts at: 6188 6450 820 Size: 262 1553 Fragments arranged by size: 2009 1553 926 901 891 262 212 197 127 105 SnaBI TAC'GTA Cuts at: 493 4974 493 Size: 4481 2702 SpeI A'CTAG_T Cuts at: 152 3959 152 Size: 3807 3376 SphI G_CATG'C Cuts at: 1132 4541 1132 Size: 3409 3774 SspI AAT'ATT Cuts at: 5 52 2701 2837 3560 3915 5107 5 Size: 47 2649 136 723 355 1192 2081 Fragments arranged by size: 2649 2081 1192 723 355 136 47 StuI AGG'CCT Cuts at: 1517 2174 1517 Size: 657 6526 StyI C'CwwG_G Cuts at: 513 1065 2004 2289 4622 513 Size: 552 939 285 2333 3074 Fragments arranged by size: 3074 2333 939 552 285 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2706 3334 5210 5227 5380 5565 6010 Size: 1255 1126 628 1876 17 153 185 445 Cuts at: 6010 325 Size: 1498 Fragments arranged by size: 1876 1498 1255 1126 628 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2916 5429 7141 Size: 274 80 33 51 1443 939 2513 1712 Cuts at: 7141 96 Size: 138 Fragments arranged by size: 2513 1712 1443 939 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2382 2414 7143 7175 2382 Size: 32 4729 32 2390 Fragments arranged by size: 4729 2390 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 7183 XbaI T'CTAG_A Cuts at: 4519 4519 Size: 7183 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2630 2630 Size: 7183 XhoI C'TCGA_G Cuts at: 1943 2070 1943 Size: 127 7056 XmaI C'CCGG_G Cuts at: 1870 4508 1870 Size: 2638 4545 XmnI GAAnn'nnTTC Cuts at: 2682 5312 2682 Size: 2630 4553 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4991 276 Size: 53 83 186 936 3457 2468 Fragments arranged by size: 3457 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoRI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PmeI PpiI PshAI PsiI PstI PvuI PvuII SalI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI ApaI AscI AvrII BaeI BbvCI BfrBI BplI BsiWI BsmBI BstXI BstZ17I EcoICRI EcoO109I EcoRV FalI FseI FspAI NruI NsiI PacI PfoI PmlI PpuMI PspOMI PspXI PsrI RsrII SacI SacII SanDI SapI SexAI SfiI SrfI SwaI