Restriction map for
pFN21AB5642
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# NO CUT ENZYMES ( 143 enzymes) #
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AccI AccIII AclI AcsI AflII
AflIII AgeI AhdI Alw44I AocI
Aor51HI ApaI ApaLI ApoI AscI
AseI AsnI Asp700I AspEI AspHI
AspI AviII AvrII BalI BamHI
BanII BbrPI BbvCI BcgI BciVI
BclI BfrI BglII BlnI BlpI
BmyI Bpu1102I BsaAI BsaBI BsaWI
BseAI BsiHKAI BsiWI BsmI Bsp1286I
Bsp1407I BspDI BspEI BspHI BspLU11I
BsrBI BsrDI BsrGI BssHII Bst1107I
BstBI BstEII BstNI BstPI BstXI
BstYI BstZ17I Bsu36I CelII ClaI
CpoI DdeI DraI DraIII DrdI
DsaI Eam1105I Eco47III Eco81I EcoNI
EcoO65I EcoRI EcoRII EcoRV EcoT22I
FbaI FseI FspI HincII HindII
HindIII HpaI KspI MamI MfeI
MflI MluI MluNI MroI MscI
MslI MunI MvaI NcoI NdeI
NheI NruI NsiI NspV PacI
PflFI PflMI PinAI PmaCI PmeI
PmlI PshAI PshBI Psp1406I PstI
PvuI PvuII RcaI RsrII SacI
SacII SalI SapI SbfI ScaI
SexAI SfcI SfiI SfuI SnaBI
SpeI Sse8387I SspBI SspI SwaI
Tsp509I TspRI Tth111I Van91I XbaI
XcmI XhoII XmnI
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# ONE CUT ENZYMES ( 30 enzymes) #
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AatII 262 GACGTC GACGT^C
Acc65I 551 GGTACC G^GTACC
AlwI 750 GATCC GGATC(4/5)
AlwNI 529 CAGTCGCTG CAG[ACGT][ACGT][ACGT]^CTG
Asp718I 551 GGTACC G^GTACC
BglI 814 GCCTTTGTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BsaI 631 GAGACC GGTCTC(1/5)
BsgI 658 GTGCAG GTGCAG(16/14)
BsmBI 673 GAGACG CGTCTC(1/5)
BssSI 670 CACGAG CACGAG(-5/-1)
BstAPI 1006 GCAGGTGTTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
DpnII 750 GATC ^GATC
EarI 367 GAAGAG CTCTTC(1/4)
KpnI 551 GGTACC GGTAC^C
Ksp632I 367 GAAGAG CTCTTC(1/4)
MboI 750 GATC ^GATC
MseI 920 TTAA T^TAA
NdeII 750 GATC ^GATC
NotI 231 GCGGCCGC GC^GGCCGC
NspI 689 GCATGC [GA]CATG^[CT]
PaeR7I 740 CTCGAG C^TCGAG
Sau3AI 750 GATC ^GATC
SmaI 90 CCCGGG CCC^GGG
SphI 689 GCATGC GCATG^C
StuI 122 AGGCCT AGG^CCT
TfiI 445 GAATC G^A[AT]TC
Tru9I 920 TTAA T^TAA
XhoI 740 CTCGAG C^TCGAG
XmaCI 90 CCCGGG C^CCGGG
XmaI 90 CCCGGG C^CCGGG
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# TWO CUT ENZYMES ( 20 enzymes) #
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AvaII 255 GGTCC G^G[AT]CC
966 GGACC
BbsI 718 GAAGAC GAAGAC(2/6)
834 GAAGAC
BfaI 916 CTAG C^TAG
970 CTAG
BpuAI 718 GAAGAC GAAGAC(2/6)
834 GAAGAC
BsmFI 908 GGGAC GGGAC(10/14)
256 GTCCC
BspMI 599 GCAGGT ACCTGC(4/8)
1006 GCAGGT
BstF5I 651 GGATG GGATG(2/0)
936 GGATG
DraII 254 AGGTCCC [GA]G^G[ACGT]CC[CT]
965 AGGACCT
EcoO109I 254 AGGTCCC [GA]G^G[ACGT]CC[CT]
965 AGGACCT
EcoT14I 578 CCAAGG C^C[AT][AT]GG
684 CCAAGG
FokI 651 GGATG GGATG(9/13)
936 GGATG
HgaI 349 GACGC GACGC(5/10)
486 GACGC
HphI 165 GGTGA GGTGA(8/7)
266 TCACC
MaeI 916 CTAG C^TAG
970 CTAG
MaeII 263 ACGT A^CGT
887 ACGT
PpuMI 254 AGGTCCC [GA]G^G[AT]CC[CT]
965 AGGACCT
SfaNI 857 GCATC GCATC(5/9)
980 GCATC
SgrAI 180 CGCCGGCG C[GA]^CCGG[CT]G
570 CACCGGCG
SmlI 322 CTTGAG C^T[CT][GA]AG
740 CTCGAG
StyI 578 CCAAGG C^C[AT][AT]GG
684 CCAAGG
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# 3 OR MORE CUT ENZYMES ( 65 enzymes) #
#########################################
AccII AciI AcyI AfaI AluI
AvaI BanI BbeI BbvI BcnI
BpmI BsaHI BsaJI BseRI BsiEI
BsiYI BslI BsmAI BsoBI BsrFI
BsrI BssKI BstUI Cac8I CfoI
Cfr10I Cfr13I EaeI EagI EclXI
Eco52I FauI Fnu4HI HaeII HaeIII
HapII HhaI Hin1I HinP1I HinfI
HpaII ItaI KasI MaeIII MboII
MnlI MspA1I MspI MvnI MwoI
NaeI NarI NciI NgoMIV NlaIII
NlaIV PleI RsaI Sau96I ScrFI
SfoI TaqI TseI Tsp45I TthHB8I