Restriction map for
pFN21AB5642
(Linear) (Six-base) MAPSORT of: KIBB5642.seq Check: 7829 from: 1 to: 1050
KIBB5642.orf
With 182 enzymes: SgfI *
February 28, 2007 11:28 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 997 1050
Size: 997 53
AatII G_ACGT'C
Cuts at: 0 266 1050
Size: 266 784
Acc65I G'GTAC_C
Cuts at: 0 551 1050
Size: 551 499
AlwNI CAG_nnn'CTG
Cuts at: 0 534 1050
Size: 534 516
AvaI C'yCGr_G
Cuts at: 0 90 400 740 1050
Size: 90 310 340 310
Fragments arranged by size:
340 310 310 90
BanI G'GyrC_C
Cuts at: 0 79 247 315 551 574 811 1050
Size: 79 168 68 236 23 237 239
Fragments arranged by size:
239 237 236 168 79 68 23
BbeI G_GCGC'C
Cuts at: 0 83 251 319 578 1050
Size: 83 168 68 259 472
Fragments arranged by size:
472 259 168 83 68
BbsI GAAGACnn'nnnn_
Cuts at: 0 725 841 1050
Size: 725 116 209
Fragments arranged by size:
725 209 116
BglI GCCn_nnn'nGGC
Cuts at: 0 820 1050
Size: 820 230
BmrI ACTGGGnnnn_n'
Cuts at: 0 789 1050
Size: 789 261
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 561 1050
Size: 561 489
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 451 556 649 1037 1050
Size: 451 105 93 388 13
Fragments arranged by size:
451 388 105 93 13
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 343 1050
Size: 343 707
BsaI GGTCTCn'nnnn_
Cuts at: 0 625 1050
Size: 625 425
BsaHI Gr'CG_yC
Cuts at: 0 80 248 263 316 350 487 575 1050
Size: 80 168 15 53 34 137 88 475
Fragments arranged by size:
475 168 137 88 80 53 34 15
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 502 532 1050
Size: 502 30 518
Fragments arranged by size:
518 502 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 340 385 517 520 523 1050
Size: 340 45 132 3 3 527
Fragments arranged by size:
527 340 132 45 3 3
BseYI C'CCAG_C
Cuts at: 0 932 1050
Size: 932 118
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 679 1050
Size: 679 371
BsiEI CG_ry'CG
Cuts at: 0 235 344 412 1050
Size: 235 109 68 638
Fragments arranged by size:
638 235 109 68
BsmBI CGTCTCn'nnnn_
Cuts at: 0 667 1050
Size: 667 383
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 590 997 1050
Size: 590 407 53
Fragments arranged by size:
590 407 53
BsrFI r'CCGG_y
Cuts at: 0 55 67 157 181 411 524 571 1050
Size: 55 12 90 24 230 113 47 479
Fragments arranged by size:
479 230 113 90 55 47 24 12
BssSI C'ACGA_G
Cuts at: 0 670 1050
Size: 670 380
BstAPI GCAn_nnn'nTGC
Cuts at: 0 1012 1050
Size: 1012 38
EaeI y'GGCC_r
Cuts at: 0 232 341 409 1050
Size: 232 109 68 641
Fragments arranged by size:
641 232 109 68
EagI C'GGCC_G
Cuts at: 0 232 341 409 1050
Size: 232 109 68 641
Fragments arranged by size:
641 232 109 68
EarI CTCTTCn'nnn_
Cuts at: 0 362 1050
Size: 362 688
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 188 1050
Size: 188 862
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 451 556 649 1037 1050
Size: 451 105 93 388 13
Fragments arranged by size:
451 388 105 93 13
EcoO109I rG'GnC_Cy
Cuts at: 0 255 966 1050
Size: 255 711 84
Fragments arranged by size:
711 255 84
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 709 1050
Size: 709 341
HaeII r_GCGC'y
Cuts at: 0 83 182 251 319 578 628 1050
Size: 83 99 69 68 259 50 422
Fragments arranged by size:
422 259 99 83 69 68 50
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 23 55 531 563 715 734 747 766
Size: 23 32 476 32 152 19 13 19
Cuts at: 766 780 812 1050
Size: 14 32 238
Fragments arranged by size:
476 238 152 32 32 32 23 19
19 14 13
KasI G'GCGC_C
Cuts at: 0 79 247 315 574 1050
Size: 79 168 68 259 476
Fragments arranged by size:
476 259 168 79 68
KpnI G_GTAC'C
Cuts at: 0 555 1050
Size: 555 495
MspA1I CmG'CkG
Cuts at: 0 74 346 426 1050
Size: 74 272 80 624
Fragments arranged by size:
624 272 80 74
NaeI GCC'GGC
Cuts at: 0 57 69 159 183 413 1050
Size: 57 12 90 24 230 637
Fragments arranged by size:
637 230 90 57 24 12
NarI GG'CG_CC
Cuts at: 0 80 248 316 575 1050
Size: 80 168 68 259 475
Fragments arranged by size:
475 259 168 80 68
NgoMIV G'CCGG_C
Cuts at: 0 55 67 157 181 411 1050
Size: 55 12 90 24 230 639
Fragments arranged by size:
639 230 90 55 24 12
NotI GC'GGCC_GC
Cuts at: 0 232 1050
Size: 232 818
NspI r_CATG'y
Cuts at: 0 693 1050
Size: 693 357
PfoI T'CCnGG_A
Cuts at: 0 217 1050
Size: 217 833
PpuMI rG'GwC_Cy
Cuts at: 0 255 966 1050
Size: 255 711 84
Fragments arranged by size:
711 255 84
SfoI GGC'GCC
Cuts at: 0 81 249 317 576 1050
Size: 81 168 68 259 474
Fragments arranged by size:
474 259 168 81 68
SgrAI Cr'CCGG_yG
Cuts at: 0 181 571 1050
Size: 181 390 479
Fragments arranged by size:
479 390 181
SmaI CCC'GGG
Cuts at: 0 92 1050
Size: 92 958
SmlI C'TyrA_G
Cuts at: 0 322 740 1050
Size: 322 418 310
Fragments arranged by size:
418 322 310
SphI G_CATG'C
Cuts at: 0 693 1050
Size: 693 357
StuI AGG'CCT
Cuts at: 0 124 1050
Size: 124 926
StyI C'CwwG_G
Cuts at: 0 578 684 1050
Size: 578 106 366
Fragments arranged by size:
578 366 106
XhoI C'TCGA_G
Cuts at: 0 740 1050
Size: 740 310
XmaI C'CCGG_G
Cuts at: 0 90 1050
Size: 90 960
ZraI GAC'GTC
Cuts at: 0 264 1050
Size: 264 786
Enzymes that do cut:
AarI AatII Acc65I AlwNI AvaI BanI BbeI BbsI
BglI BmrI BplI BpmI BpuEI BsaI BsaHI BsaXI
BseRI BseYI BsgI BsiEI BsmBI BspMI BsrFI BssSI
BstAPI EaeI EagI EarI EciI Eco57MI EcoO109I FalI
HaeII Hin4I KasI KpnI MspA1I NaeI NarI NgoMIV
NotI NspI PfoI PpuMI SfoI SgrAI SmaI SmlI
SphI StuI StyI XhoI XmaI ZraI
Enzymes that do not cut:
AccI AclI AcuI AfeI AflII AflIII AgeI AhdI
AleI AlfI AloI ApaI ApaLI ApoI AscI AseI
AsiSI AvrII BaeI BamHI BanII BbvCI BcgI BciVI
BclI BfrBI BglII BlpI Bme1580I BmgBI BmtI Bpu10I
BsaAI BsaBI BsaWI BsiHKAI BsiWI BsmI Bsp1286I BspEI
BspHI BsrBI BsrDI BsrGI BssHII BstBI BstEII BstXI
BstYI BstZ17I Bsu36I BtgI BtgZI BtsI ClaI CspCI
DraI DraIII DrdI EcoICRI EcoNI EcoRI EcoRV FseI
FspI FspAI HincII HindIII HpaI MfeI MluI MmeI
MscI MslI NcoI NdeI NheI NruI NsiI PacI
PasI PciI PflMI PmeI PmlI PpiI PshAI PsiI
PspOMI PspXI PsrI PstI PvuI PvuII RsrII SacI
SacII SalI SanDI SapI SbfI ScaI SexAI SfcI
SfiI SgfI SnaBI SpeI SrfI SspI SwaI TaqII
TatI TstI Tth111I XbaI XcmI XmnI