(Circular) (Six-base) MAPSORT of: pFN21AB5631.seq Check: 3952 from: 1 to: 7813 pFN21AB5631 7813 bp With 182 enzymes: SgfI * February 27, 2009 14:34 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 3553 3723 3553 Size: 170 7643 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 5623 278 Size: 53 83 186 936 4087 2468 Fragments arranged by size: 4087 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2124 2838 3325 5156 1937 Size: 187 714 487 1831 4594 Fragments arranged by size: 4594 1831 714 487 187 Acc65I G'GTAC_C Cuts at: 2293 5134 2293 Size: 2841 4972 AclI AA'CG_TT Cuts at: 1563 5940 6313 1563 Size: 4377 373 3063 Fragments arranged by size: 4377 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3501 4689 5021 5070 5876 7260 7528 1632 Size: 1869 1188 332 49 806 1384 268 1917 Fragments arranged by size: 1917 1869 1384 1188 806 332 268 49 AfeI AGC'GCT Cuts at: 3989 3989 Size: 7813 AflII C'TTAA_G Cuts at: 820 1017 3571 820 Size: 197 2554 5062 Fragments arranged by size: 5062 2554 197 AflIII A'CryG_T Cuts at: 1203 2133 2442 6712 7689 1203 Size: 930 309 4270 977 1327 Fragments arranged by size: 4270 1327 977 930 309 AgeI A'CCGG_T Cuts at: 6644 6644 Size: 7813 AhdI GACnn_n'nnGTC Cuts at: 6542 6542 Size: 7813 AleI CACnn'nnGTG Cuts at: 1166 3491 1166 Size: 2325 5488 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2070 3948 1184 Size: 886 1878 5049 Fragments arranged by size: 5049 1878 886 AlwNI CAG_nnn'CTG Cuts at: 3819 4697 5556 7128 3819 Size: 878 859 1572 4504 Fragments arranged by size: 4504 1572 878 859 ApaLI G'TGCA_C Cuts at: 5870 7026 5870 Size: 1156 6657 ApoI r'AATT_y Cuts at: 1493 2336 2360 2378 2846 2978 3680 4453 4832 Size: 843 24 18 468 132 702 773 379 Cuts at: 4832 5122 5348 5500 7609 1493 Size: 290 226 152 2109 1697 Fragments arranged by size: 2109 1697 843 773 702 468 379 290 226 152 132 24 18 AseI AT'TA_AT Cuts at: 160 1032 2304 6367 160 Size: 872 1272 4063 1606 Fragments arranged by size: 4063 1606 1272 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 7813 AvaI C'yCGr_G Cuts at: 1870 1943 2475 4746 5138 1870 Size: 73 532 2271 392 4545 Fragments arranged by size: 4545 2271 532 392 73 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 2210 2243 2277 2310 2210 Size: 33 34 33 7713 Fragments arranged by size: 7713 34 33 33 BamHI G'GATC_C Cuts at: 4302 4816 5143 5510 4302 Size: 514 327 367 6605 Fragments arranged by size: 6605 514 367 327 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2293 2813 2996 3708 4899 Size: 325 217 627 506 520 183 712 1191 Cuts at: 4899 5134 6589 618 Size: 235 1455 1842 Fragments arranged by size: 1842 1455 1191 712 627 520 506 325 235 217 183 BanII G_rGCy'C Cuts at: 1391 3391 4542 5132 1391 Size: 2000 1151 590 4072 Fragments arranged by size: 4072 2000 1151 590 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2443 3841 928 Size: 971 544 1398 4900 Fragments arranged by size: 4900 1398 971 544 BbvCI CC'TCA_GC Cuts at: 4989 4989 Size: 7813 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4274 4308 6004 6038 1903 Size: 34 2337 34 1696 34 3678 Fragments arranged by size: 3678 2337 1696 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 5705 6915 5705 Size: 1210 6603 BclI T'GATC_A Cuts at: 1548 1548 Size: 7813 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 6424 136 Size: 107 122 71 631 605 4752 1525 Fragments arranged by size: 4752 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 2690 7808 2690 Size: 5118 2695 BlpI GC'TnA_GC Cuts at: 5230 5230 Size: 7813 Bme1580I G_kGCm'C Cuts at: 1790 5097 5874 7030 1790 Size: 3307 777 1156 2573 Fragments arranged by size: 3307 2573 1156 777 BmgBI CAC'GTC Cuts at: 1318 2134 3493 3974 1318 Size: 816 1359 481 5157 Fragments arranged by size: 5157 1359 816 481 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2481 3173 3868 Size: 416 53 83 399 24 1064 692 695 Cuts at: 3868 5544 6502 442 Size: 1676 958 1753 Fragments arranged by size: 1753 1676 1064 958 695 692 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 7813 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2411 2411 Size: 7813 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2715 6473 1239 Size: 1476 3758 2579 Fragments arranged by size: 3758 2579 1476 Bpu10I CC'TnA_GC Cuts at: 4968 4989 4968 Size: 21 7792 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2181 2381 5938 6803 7101 2181 Size: 200 3557 865 298 2893 Fragments arranged by size: 3557 2893 865 298 200 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 6476 882 Size: 617 4977 2219 Fragments arranged by size: 4977 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 5604 493 Size: 711 4400 2702 Fragments arranged by size: 4400 2702 711 BsaBI GATnn'nnATC Cuts at: 3134 7807 3134 Size: 4673 3140 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 5620 6002 6637 275 Size: 53 83 186 936 4087 382 635 1451 Fragments arranged by size: 4087 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4896 6246 6644 6918 7065 7476 4896 Size: 1350 398 274 147 411 5233 Fragments arranged by size: 5233 1350 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2106 2136 3737 3767 3887 Size: 30 165 30 703 30 1601 30 120 Cuts at: 3887 3917 4107 4137 1178 Size: 30 190 30 4854 Fragments arranged by size: 4854 1601 703 190 165 120 30 30 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2132 2483 4461 5006 1186 Size: 946 351 1978 545 3993 Fragments arranged by size: 3993 1978 946 545 351 BseYI C'CCAG_C Cuts at: 2141 4648 7016 7512 2141 Size: 2507 2368 496 2442 Fragments arranged by size: 2507 2442 2368 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2025 3701 4462 1163 Size: 573 289 1676 761 4514 Fragments arranged by size: 4514 1676 761 573 289 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 5263 6024 7052 1540 Size: 275 186 3262 761 1028 2301 Fragments arranged by size: 3262 2301 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 3480 4542 5874 5959 7030 3480 Size: 1062 1332 85 1071 4263 Fragments arranged by size: 4263 1332 1071 1062 85 BsmI GAATG_Cn' Cuts at: 3055 3213 5324 5417 3055 Size: 158 2111 93 5451 Fragments arranged by size: 5451 2111 158 93 BsmBI CGTCTCn'nnnn_ Cuts at: 3531 3531 Size: 7813 Bsp1286I G_dGCh'C Cuts at: 1391 1790 3391 3480 4542 5097 5132 5874 5959 Size: 399 1601 89 1062 555 35 742 85 Cuts at: 5959 7030 1391 Size: 1071 2174 Fragments arranged by size: 2174 1601 1071 1062 742 555 399 89 85 35 BspEI T'CCGG_A Cuts at: 7476 7476 Size: 7813 BspHI T'CATG_A Cuts at: 5702 7432 5702 Size: 1730 6083 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3553 3723 5168 844 Size: 2709 170 1445 3489 Fragments arranged by size: 3489 2709 1445 170 BsrBI CCG'CTC Cuts at: 1394 1823 4128 5700 1394 Size: 429 2305 1572 3507 Fragments arranged by size: 3507 2305 1572 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2817 3114 3558 3930 4290 6308 6482 Size: 1183 1569 297 444 372 360 2018 174 Cuts at: 6482 65 Size: 1396 Fragments arranged by size: 2018 1569 1396 1183 444 372 360 297 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4388 4737 6457 6644 1700 Size: 110 2578 349 1720 187 2869 Fragments arranged by size: 2869 2578 1720 349 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 7813 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 7813 BssSI C'ACGA_G Cuts at: 3960 5873 6885 3960 Size: 1913 1012 4888 Fragments arranged by size: 4888 1913 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 3715 794 Size: 2921 4892 BstBI TT'CG_AA Cuts at: 4371 6617 4371 Size: 2246 5567 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 7813 BstXI CCAn_nnnn'nTGG Cuts at: 2993 2993 Size: 7813 BstYI r'GATC_y Cuts at: 1972 2690 3246 4302 4816 4975 5007 5143 5510 Size: 718 556 1056 514 159 32 136 367 Cuts at: 5510 5895 5912 7364 7450 7462 7808 1972 Size: 385 17 1452 86 12 346 1977 Fragments arranged by size: 1977 1452 1056 718 556 514 385 367 346 159 136 86 32 17 12 BtgI C'CryG_G Cuts at: 513 1065 2024 3946 5011 5097 513 Size: 552 959 1922 1065 86 3229 Fragments arranged by size: 3229 1922 1065 959 552 86 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 4299 487 Size: 683 56 3073 4001 Fragments arranged by size: 4001 3073 683 56 BtsI GCAGTG_nn' Cuts at: 2042 3505 5329 6123 6143 2042 Size: 1463 1824 794 20 3712 Fragments arranged by size: 3712 1824 1463 794 20 ClaI AT'CG_AT Cuts at: 1554 3019 1554 Size: 1465 6348 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 7778 DraI TTT'AAA Cuts at: 2341 2928 5117 2341 Size: 587 2189 5037 Fragments arranged by size: 5037 2189 587 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 6820 809 Size: 507 5504 1802 Fragments arranged by size: 5504 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 4735 5260 6149 Size: 54 1425 50 275 18 2905 525 889 Cuts at: 6149 7778 8 Size: 1629 43 Fragments arranged by size: 2905 1629 1425 889 525 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 5260 1537 Size: 275 3448 4090 Fragments arranged by size: 4090 3448 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2731 3108 3120 3614 4161 5743 1353 Size: 1378 377 12 494 547 1582 3423 Fragments arranged by size: 3423 1582 1378 547 494 377 12 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4407 6400 6774 6920 387 Size: 1064 138 2818 1993 374 146 1280 Fragments arranged by size: 2818 1993 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 4540 4540 Size: 7813 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2715 3501 4689 5021 5070 5876 6473 Size: 393 1083 786 1188 332 49 806 597 Cuts at: 6473 7260 7528 1239 Size: 787 268 1524 Fragments arranged by size: 1524 1188 1083 806 787 786 597 393 332 268 49 EcoNI CCTnn'n_nnAGG Cuts at: 3457 3457 Size: 7813 EcoRI G'AATT_C Cuts at: 2846 2978 5122 5500 2846 Size: 132 2144 378 5159 Fragments arranged by size: 5159 2144 378 132 EcoRV GAT'ATC Cuts at: 4085 4085 Size: 7813 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 4499 4499 Size: 7813 FspI TGC'GCA Cuts at: 6319 6319 Size: 7813 HaeII r_GCGC'y Cuts at: 1678 3991 6960 1678 Size: 2313 2969 2531 Fragments arranged by size: 2969 2531 2313 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 3231 3263 3350 3382 3737 Size: 32 135 32 1659 32 87 32 355 Cuts at: 3737 3769 3887 3919 3920 3952 6454 6486 6528 Size: 32 118 32 1 32 2502 32 42 Cuts at: 6528 6560 1373 Size: 32 2626 Fragments arranged by size: 2626 2502 1659 355 135 118 87 42 32 32 32 32 32 32 32 32 32 1 HincII GTy'rAC Cuts at: 669 1657 1938 5157 5403 669 Size: 988 281 3219 246 3079 Fragments arranged by size: 3219 3079 988 281 246 HindIII A'AGCT_T Cuts at: 748 3401 748 Size: 2653 5160 HpaI GTT'AAC Cuts at: 5403 5403 Size: 7813 KpnI G_GTAC'C Cuts at: 2297 5138 2297 Size: 2841 4972 MfeI C'AATT_G Cuts at: 5412 5412 Size: 7813 MluI A'CGCG_T Cuts at: 7689 7689 Size: 7813 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 3179 3186 6927 7111 1305 Size: 1874 7 3741 184 2007 Fragments arranged by size: 3741 2007 1874 184 7 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 5991 Fragments arranged by size: 5991 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 3341 3491 5771 6130 6289 518 Size: 648 2175 150 2280 359 159 2042 Fragments arranged by size: 2280 2175 2042 648 359 159 150 MspA1I CmG'CkG Cuts at: 1606 2635 2811 4936 5229 5906 7054 7299 1606 Size: 1029 176 2125 293 677 1148 245 2120 Fragments arranged by size: 2125 2120 1148 1029 677 293 245 176 NcoI C'CATG_G Cuts at: 513 1065 5097 513 Size: 552 4032 3229 Fragments arranged by size: 4032 3229 552 NdeI CA'TA_TG Cuts at: 387 3031 387 Size: 2644 5169 NheI G'CTAG_C Cuts at: 1052 1052 Size: 7813 NotI GC'GGCC_GC Cuts at: 5260 5260 Size: 7813 NspI r_CATG'y Cuts at: 1132 1229 2951 4535 4648 5171 6716 1132 Size: 97 1722 1584 113 523 1545 2229 Fragments arranged by size: 2229 1722 1584 1545 523 113 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 7813 PciI A'CATG_T Cuts at: 6712 6712 Size: 7813 PflMI CCAn_nnn'nTGG Cuts at: 1108 2653 3424 3819 1108 Size: 1545 771 395 5102 Fragments arranged by size: 5102 1545 771 395 PmeI GTTT'AAAC Cuts at: 5117 5117 Size: 7813 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 5882 5914 7395 7427 5882 Size: 32 1481 32 6268 Fragments arranged by size: 6268 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 7813 PsiI TTA'TAA Cuts at: 2190 4949 5383 2190 Size: 2759 434 4620 Fragments arranged by size: 4620 2759 434 PstI C_TGCA'G Cuts at: 830 2049 3943 5165 830 Size: 1219 1894 1222 3478 Fragments arranged by size: 3478 1894 1222 1219 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 7813 PvuII CAG'CTG Cuts at: 2635 4936 2635 Size: 2301 5512 SacI G_AGCT'C Cuts at: 4542 4542 Size: 7813 SalI G'TCGA_C Cuts at: 1936 5155 1936 Size: 3219 4594 SapI GCTCTTCn'nnn_ Cuts at: 2731 2731 Size: 7813 SbfI CC_TGCA'GG Cuts at: 5165 5165 Size: 7813 ScaI AGT'ACT Cuts at: 6061 7773 6061 Size: 1712 6101 SfcI C'TryA_G Cuts at: 826 1045 2045 2746 2941 2970 3939 4406 4429 Size: 219 1000 701 195 29 969 467 23 Cuts at: 4429 4760 5161 6296 6977 7168 826 Size: 331 401 1135 681 191 1471 Fragments arranged by size: 1471 1135 1000 969 701 681 467 401 331 219 195 191 29 23 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 7813 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 7813 SmaI CCC'GGG Cuts at: 1872 5140 1872 Size: 3268 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2196 2396 2475 3571 5917 6818 Size: 197 926 253 200 79 1096 2346 901 Cuts at: 6818 7080 820 Size: 262 1553 Fragments arranged by size: 2346 1553 1096 926 901 262 253 200 197 79 SnaBI TAC'GTA Cuts at: 493 5604 493 Size: 5111 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 7813 SphI G_CATG'C Cuts at: 1132 4648 5171 1132 Size: 3516 523 3774 Fragments arranged by size: 3774 3516 523 SspI AAT'ATT Cuts at: 5 52 5737 5 Size: 47 5685 2081 Fragments arranged by size: 5685 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 7813 StyI C'CwwG_G Cuts at: 513 1065 2533 4462 4780 5097 5252 513 Size: 552 1468 1929 318 317 155 3074 Fragments arranged by size: 3074 1929 1468 552 318 317 155 SwaI ATTT'AAAT Cuts at: 2341 2341 Size: 7813 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 4014 5840 5857 6010 6195 6640 325 Size: 1255 2434 1826 17 153 185 445 1498 Fragments arranged by size: 2434 1826 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2400 4918 6059 Size: 274 80 33 51 1443 423 2518 1141 Cuts at: 6059 7771 96 Size: 1712 138 Fragments arranged by size: 2518 1712 1443 1141 423 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5007 5039 7773 7805 5007 Size: 32 2734 32 5015 Fragments arranged by size: 5015 2734 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 7813 XbaI T'CTAG_A Cuts at: 5149 5149 Size: 7813 XcmI CCAnnnn_n'nnnnTGG Cuts at: 5095 5095 Size: 7813 XhoI C'TCGA_G Cuts at: 1943 2475 1943 Size: 532 7281 XmaI C'CCGG_G Cuts at: 1870 5138 1870 Size: 3268 4545 XmnI GAAnn'nnTTC Cuts at: 5942 5942 Size: 7813 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 5621 276 Size: 53 83 186 936 4087 2468 Fragments arranged by size: 4087 2468 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BaeI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoRI EcoRV FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PpiI PshAI PsiI PstI PvuI PvuII SacI SalI SapI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI SwaI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AloI ApaI AscI AvrII BbeI BfrBI BsiWI BstZ17I Bsu36I DraIII EcoO109I FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PfoI PmlI PpuMI PspOMI PspXI PsrI RsrII SacII SanDI SexAI SfiI SfoI SrfI