Restriction map for
pFN21AB5625
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# NO CUT ENZYMES ( 87 enzymes) #
##################################
AatII Acc65I AflIII AgeI AhdI
Alw44I Aor51HI ApaI ApaLI AscI
Asp718I AspEI AviII BamHI BbeI
BbrPI BglI BlpI Bpu1102I BsaAI
BsaBI BsiEI BsiWI BsmBI BspDI
BspLU11I BsrBI BssHII Bst1107I BstAPI
BstBI BstEII BstPI BstXI BstZ17I
CelII ClaI CpoI DraIII DrdI
EagI Eam1105I EclXI Eco47III Eco52I
EcoO65I EcoRI EcoT22I FseI FspI
KasI KpnI KspI MamI MluI
NaeI NarI NgoMIV NheI NotI
NruI NsiI NspV PacI PflMI
PinAI PmaCI PmeI PmlI PpuMI
PvuI RsrII SacII SalI SbfI
SexAI SfiI SfoI SfuI SmaI
SnaBI SpeI Sse8387I SwaI Van91I
XmaCI XmaI
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# ONE CUT ENZYMES ( 57 enzymes) #
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AccII 3149 CGCG CG^CG
AccIII 3344 TCCGGA T^CCGGA
AclI 3485 AACGTT AA^CGTT
AcyI 3215 GACGCC G[GA]^CG[CT]C
AflII 1810 CTTAAG C^TTAAG
AocI 1202 CCTCAGG CC^T[ACGT]AGG
AseI 2509 ATTAAT AT^TAAT
AsnI 2509 ATTAAT AT^TAAT
AvrII 2784 CCTAGG C^CTAGG
BanI 1520 GGTGCC G^G[CT][GA]CC
BbvCI 111 CCTCAGC CCTCAGC(-5/-2)
BcgI 514 GCATCTGGCTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BciVI 1706 GTATCC GTATCC(6/5)
BfrI 1810 CTTAAG C^TTAAG
BglII 1747 AGATCT A^GATCT
BlnI 2784 CCTAGG C^CTAGG
BsaHI 3215 GACGCC G[GA]^CG[CT]C
BseAI 3344 TCCGGA T^CCGGA
BsgI 1426 GTGCAG GTGCAG(16/14)
Bsp1407I 776 TGTACA T^GTACA
BspEI 3344 TCCGGA T^CCGGA
BsrDI 1765 GCAATG GCAATG(2/0)
BsrFI 1286 ACCGGC [GA]^CCGG[CT]
BsrGI 776 TGTACA T^GTACA
BssSI 1004 CACGAG CACGAG(-5/-1)
BstUI 3149 CGCG CG^CG
Bsu36I 1202 CCTCAGG CC^T[ACGT]AGG
CfoI 652 GCGC GCG^C
Cfr10I 1286 ACCGGC [GA]^CCGG[CT]
DraII 412 AGGGCCT [GA]G^G[ACGT]CC[CT]
Eco81I 1202 CCTCAGG CC^T[ACGT]AGG
EcoNI 1191 CCTCAGACAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO109I 412 AGGGCCT [GA]G^G[ACGT]CC[CT]
EcoRV 359 GATATC GAT^ATC
FauI 567 CCCGC CCCGC(4/6)
HaeII 651 GGCGCT [GA]GCGC^[CT]
HhaI 652 GCGC GCG^C
Hin1I 3215 GACGCC G[GA]^CG[CT]C
HinP1I 652 GCGC G^CGC
MfeI 2082 CAATTG C^AATTG
MroI 3344 TCCGGA T^CCGGA
MunI 2082 CAATTG C^AATTG
MvnI 3149 CGCG CG^CG
NcoI 2834 CCATGG C^CATGG
NdeI 1073 CATATG CA^TATG
NspI 599 GCATGC [GA]CATG^[CT]
PaeR7I 1801 CTCGAG C^TCGAG
PshAI 2080 GACAATTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PshBI 2509 ATTAAT AT^TAAT
Psp1406I 3485 AACGTT AA^CGTT
PstI 1456 CTGCAG CTGCA^G
SacI 921 GAGCTC GAGCT^C
ScaI 719 AGTACT AGT^ACT
SgrAI 1285 CACCGGCG C[GA]^CCGG[CT]G
SphI 599 GCATGC GCATG^C
SspBI 776 TGTACA T^GTACA
XhoI 1801 CTCGAG C^TCGAG
###################################
# TWO CUT ENZYMES ( 26 enzymes) #
###################################
AccI 481 GTCTAC GT^[AC][GT]AC
2950 GTCTAC
AfaI 720 GTAC GT^AC
777 GTAC
Asp700I 209 GAAGCCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
2958 GAATAGTTTC
AspI 475 GACAATGTC GAC[ACGT]^[ACGT][ACGT]GTC
1340 GACAGCGTC
AvaI 1089 CCCGAG C^[CT]CG[GA]G
1801 CTCGAG
AvaII 1409 GGACC G^G[AT]CC
2991 GGACC
BsaI 1351 GAGACC GGTCTC(1/5)
3037 GAGACC
BsoBI 1089 CCCGAG C^[CT]CG[GA]G
1801 CTCGAG
BspHI 1496 TCATGA T^CATGA
3657 TCATGA
BspMI 3335 ACCTGC ACCTGC(4/8)
3433 ACCTGC
DraI 145 TTTAAA TTT^AAA
2478 TTTAAA
DsaI 1415 CCGTGG C^C[GA][CT]GG
2834 CCATGG
HincII 666 GTTAAC GT[CT]^[GA]AC
2464 GTTAAC
HindII 666 GTTAAC GT[CT]^[GA]AC
2464 GTTAAC
HindIII 1679 AAGCTT A^AGCTT
2011 AAGCTT
HpaI 666 GTTAAC GTT^AAC
2464 GTTAAC
PflFI 475 GACAATGTC GAC[ACGT]^[ACGT][ACGT]GTC
1340 GACAGCGTC
RcaI 1496 TCATGA T^CATGA
3657 TCATGA
RsaI 720 GTAC GT^AC
777 GTAC
SapI 4 GAAGAGC GCTCTTC(1/4)
371 GAAGAGC
SfcI 753 CTACAG C^T[GA][CT]AG
1456 CTGCAG
Tsp45I 740 GTCAC ^GT[GC]AC
1634 GTGAC
Tth111I 475 GACAATGTC GAC[ACGT]^[ACGT][ACGT]GTC
1340 GACAGCGTC
XbaI 2205 TCTAGA T^CTAGA
2278 TCTAGA
XcmI 998 CCAGTCCACGAGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
2594 CCACAGCTGTGCTGG
XmnI 209 GAAGCCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
2958 GAATAGTTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 88 enzymes) #
#########################################
AciI AcsI AluI AlwI AlwNI
ApoI AspHI BalI BanII BbsI
BbvI BclI BcnI BfaI BmyI
BpmI BpuAI BsaJI BsaWI BseRI
BsiHKAI BsiYI BslI BsmAI BsmFI
BsmI Bsp1286I BsrI BssKI BstF5I
BstNI BstYI Cac8I Cfr13I DdeI
DpnII EaeI EarI EcoRII EcoT14I
FbaI Fnu4HI FokI HaeIII HapII
HgaI HinfI HpaII HphI ItaI
Ksp632I MaeI MaeII MaeIII MboI
MboII MflI MluNI MnlI MscI
MseI MslI MspA1I MspI MvaI
MwoI NciI NdeII NlaIII NlaIV
PleI PvuII Sau3AI Sau96I ScrFI
SfaNI SmlI SspI StuI StyI
TaqI TfiI Tru9I TseI Tsp509I
TspRI TthHB8I XhoII