(Circular) (Six-base) MAPSORT of: pFN21AB5625.seq Check: 7762 from: 1 to: 8464 pFN21AB5625 8464 bp With 182 enzymes: SgfI * January 16, 2009 15:25 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 6274 278 Size: 53 83 186 936 4738 2468 Fragments arranged by size: 4738 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2487 4956 5807 1937 Size: 550 2469 851 4594 Fragments arranged by size: 4594 2469 851 550 Acc65I G'GTAC_C Cuts at: 5785 5785 Size: 8464 AclI AA'CG_TT Cuts at: 1563 5491 6591 6964 1563 Size: 3928 1100 373 3063 Fragments arranged by size: 3928 3063 1100 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2982 4034 4148 6527 7911 8179 1632 Size: 1350 1052 114 2379 1384 268 1917 Fragments arranged by size: 2379 1917 1384 1350 1052 268 114 AflII C'TTAA_G Cuts at: 820 1017 3815 820 Size: 197 2798 5469 Fragments arranged by size: 5469 2798 197 AflIII A'CryG_T Cuts at: 1203 7363 8340 1203 Size: 6160 977 1327 Fragments arranged by size: 6160 1327 977 AgeI A'CCGG_T Cuts at: 7295 7295 Size: 8464 AhdI GACnn_n'nnGTC Cuts at: 7193 7193 Size: 8464 AleI CACnn'nnGTG Cuts at: 1166 4604 1166 Size: 3438 5026 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 5157 1184 Size: 3973 4491 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2536 2568 2536 Size: 32 8432 AlwNI CAG_nnn'CTG Cuts at: 3352 3372 4246 4943 6207 7779 3352 Size: 20 874 697 1264 1572 4037 Fragments arranged by size: 4037 1572 1264 874 697 20 ApaLI G'TGCA_C Cuts at: 6521 7677 6521 Size: 1156 7308 ApoI r'AATT_y Cuts at: 1493 3170 4296 4922 5773 5999 6151 8260 1493 Size: 1677 1126 626 851 226 152 2109 1697 Fragments arranged by size: 2109 1697 1677 1126 851 626 226 152 AseI AT'TA_AT Cuts at: 160 1032 4515 7018 160 Size: 872 3483 2503 1606 Fragments arranged by size: 3483 2503 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 8464 AvaI C'yCGr_G Cuts at: 1870 1943 3094 3806 5789 1870 Size: 73 1151 712 1983 4545 Fragments arranged by size: 4545 1983 1151 712 73 AvrII C'CTAG_G Cuts at: 4789 4789 Size: 8464 BamHI G'GATC_C Cuts at: 5794 6161 5794 Size: 367 8097 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3525 5785 7240 618 Size: 325 217 627 1738 2260 1455 1842 Fragments arranged by size: 2260 1842 1738 1455 627 325 217 BanII G_rGCy'C Cuts at: 1391 2930 3509 5203 5783 1391 Size: 1539 579 1694 580 4072 Fragments arranged by size: 4072 1694 1539 580 579 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2070 4079 4179 5364 5441 928 Size: 971 171 2009 100 1185 77 3951 Fragments arranged by size: 3951 2009 1185 971 171 100 77 BbvCI CC'TCA_GC Cuts at: 2117 2117 Size: 8464 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 2508 2542 6655 6689 1903 Size: 34 571 34 4113 34 3678 Fragments arranged by size: 4113 3678 571 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3722 6356 7566 3722 Size: 2634 1210 4620 Fragments arranged by size: 4620 2634 1210 BclI T'GATC_A Cuts at: 1548 2389 3067 3841 5602 1548 Size: 841 678 774 1761 4410 Fragments arranged by size: 4410 1761 841 774 678 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 7075 136 Size: 107 122 71 631 605 5403 1525 Fragments arranged by size: 5403 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 3752 8459 3752 Size: 4707 3757 BlpI GC'TnA_GC Cuts at: 5881 5881 Size: 8464 Bme1580I G_kGCm'C Cuts at: 1790 5317 6525 7681 1790 Size: 3527 1208 1156 2573 Fragments arranged by size: 3527 2573 1208 1156 BmgBI CAC'GTC Cuts at: 1318 3269 1318 Size: 1951 6513 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3420 6195 7153 Size: 416 53 83 399 24 2003 2775 958 Cuts at: 7153 442 Size: 1753 Fragments arranged by size: 2775 2003 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 8464 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2083 4078 5468 2083 Size: 1995 1390 5079 Fragments arranged by size: 5079 1995 1390 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2713 3135 3374 4199 4423 7124 1239 Size: 1474 422 239 825 224 2701 2579 Fragments arranged by size: 2701 2579 1474 825 422 239 224 Bpu10I CC'TnA_GC Cuts at: 2117 2257 2929 2117 Size: 140 672 7652 Fragments arranged by size: 7652 672 140 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2443 2446 3473 5594 6589 7454 7752 2443 Size: 3 1027 2121 995 865 298 3155 Fragments arranged by size: 3155 2121 1027 995 865 298 3 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 3350 5036 7127 882 Size: 617 1851 1686 2091 2219 Fragments arranged by size: 2219 2091 1851 1686 617 BsaAI yAC'GTr Cuts at: 493 1204 6255 493 Size: 711 5051 2702 Fragments arranged by size: 5051 2702 711 BsaBI GATnn'nnATC Cuts at: 8458 8458 Size: 8464 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 5221 6271 6653 7288 Size: 53 83 186 936 3688 1050 382 635 Cuts at: 7288 275 Size: 1451 Fragments arranged by size: 3688 1451 1050 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 3531 3957 5349 6897 7295 7569 7716 8127 3531 Size: 426 1392 1548 398 274 147 411 3868 Fragments arranged by size: 3868 1548 1392 426 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 3470 3500 3565 3595 3637 Size: 30 165 30 2067 30 65 30 42 Cuts at: 3637 3667 1178 Size: 30 5975 Fragments arranged by size: 5975 2067 165 65 42 30 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 3034 3491 3645 1186 Size: 1848 457 154 6005 Fragments arranged by size: 6005 1848 457 154 BseYI C'CCAG_C Cuts at: 3219 4077 7667 8163 3219 Size: 858 3590 496 3520 Fragments arranged by size: 3590 3520 858 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 3452 1163 Size: 573 1716 6175 Fragments arranged by size: 6175 1716 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 5914 6675 7703 1540 Size: 275 186 3913 761 1028 2301 Fragments arranged by size: 3913 2301 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2930 3617 3886 4638 4821 6525 6610 7681 2930 Size: 687 269 752 183 1704 85 1071 3713 Fragments arranged by size: 3713 1704 1071 752 687 269 183 85 BsmI GAATG_Cn' Cuts at: 2077 2640 5620 5975 6068 2077 Size: 563 2980 355 93 4473 Fragments arranged by size: 4473 2980 563 355 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2930 3509 3617 3886 4638 4821 5203 Size: 399 1140 579 108 269 752 183 382 Cuts at: 5203 5317 5783 6525 6610 7681 1391 Size: 114 466 742 85 1071 2174 Fragments arranged by size: 2174 1140 1071 752 742 579 466 399 382 269 183 114 108 85 BspEI T'CCGG_A Cuts at: 5349 8127 5349 Size: 2778 5686 BspHI T'CATG_A Cuts at: 3501 5662 6353 8083 3501 Size: 2161 691 1730 3882 Fragments arranged by size: 3882 2161 1730 691 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 5349 5447 5819 844 Size: 4505 98 372 3489 Fragments arranged by size: 4505 3489 372 98 BsrBI CCG'CTC Cuts at: 1394 1823 6351 1394 Size: 429 4528 3507 Fragments arranged by size: 4528 3507 429 BsrDI GCAATG_nn' Cuts at: 65 1248 3777 6959 7133 65 Size: 1183 2529 3182 174 1396 Fragments arranged by size: 3182 2529 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 3291 7108 7295 1700 Size: 110 1481 3817 187 2869 Fragments arranged by size: 3817 2869 1481 187 110 BsrGI T'GTAC_A Cuts at: 96 2781 96 Size: 2685 5779 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 8464 BssSI C'ACGA_G Cuts at: 3009 6524 7536 3009 Size: 3515 1012 3937 Fragments arranged by size: 3937 3515 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 8464 BstBI TT'CG_AA Cuts at: 7268 7268 Size: 8464 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 8464 BstYI r'GATC_y Cuts at: 1972 2872 3161 3752 5709 5794 6161 6546 6563 Size: 900 289 591 1957 85 367 385 17 Cuts at: 6563 8015 8101 8113 8459 1972 Size: 1452 86 12 346 1977 Fragments arranged by size: 1977 1957 1452 900 591 385 367 346 289 86 85 17 12 Bsu36I CC'TnA_GG Cuts at: 3208 3208 Size: 8464 BtgI C'CryG_G Cuts at: 513 1065 2004 3420 4839 513 Size: 552 939 1416 1419 4138 Fragments arranged by size: 4138 1419 1416 939 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 5676 487 Size: 683 56 4450 3275 Fragments arranged by size: 4450 3275 683 56 BtsI GCAGTG_nn' Cuts at: 3595 4750 5980 6774 6794 3595 Size: 1155 1230 794 20 5265 Fragments arranged by size: 5265 1230 1155 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 8464 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 5446 5481 529 Size: 35 4882 35 3512 Fragments arranged by size: 4882 3512 35 35 DraI TTT'AAA Cuts at: 2152 4485 5768 2152 Size: 2333 1283 4848 Fragments arranged by size: 4848 2333 1283 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 7471 809 Size: 507 6155 1802 Fragments arranged by size: 6155 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2401 2535 4842 Size: 54 1425 50 275 18 571 134 2307 Cuts at: 4842 5392 5911 6800 8429 8 Size: 550 519 889 1629 43 Fragments arranged by size: 2307 1629 1425 889 571 550 519 275 134 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 5911 1537 Size: 275 4099 4090 Fragments arranged by size: 4099 4090 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2004 2140 2371 4124 6394 1353 Size: 651 136 231 1753 2270 3423 Fragments arranged by size: 3423 2270 1753 651 231 136 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 7051 7425 7571 387 Size: 1064 138 5462 374 146 1280 Fragments arranged by size: 5462 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2928 2928 Size: 8464 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2713 2982 3135 3374 4034 4148 4199 Size: 393 1081 269 153 239 660 114 51 Cuts at: 4199 4423 6527 7124 7911 8179 1239 Size: 224 2104 597 787 268 1524 Fragments arranged by size: 2104 1524 1081 787 660 597 393 269 268 239 224 153 114 51 EcoNI CCTnn'n_nnAGG Cuts at: 3200 3200 Size: 8464 EcoO109I rG'GnC_Cy Cuts at: 2418 2418 Size: 8464 EcoRI G'AATT_C Cuts at: 5773 6151 5773 Size: 378 8086 EcoRV GAT'ATC Cuts at: 2366 2366 Size: 8464 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2625 4246 5377 2625 Size: 1621 1131 5712 Fragments arranged by size: 5712 1621 1131 FspI TGC'GCA Cuts at: 6970 6970 Size: 8464 HaeII r_GCGC'y Cuts at: 1678 2660 7611 1678 Size: 982 4951 2531 Fragments arranged by size: 4951 2531 982 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2678 2710 3271 3303 3334 Size: 32 135 32 1106 32 561 32 31 Cuts at: 3334 3366 3464 3496 3565 3597 3931 3963 4841 Size: 32 98 32 69 32 334 32 878 Cuts at: 4841 4873 5060 5092 5439 5471 5617 5649 7105 Size: 32 187 32 347 32 146 32 1456 Cuts at: 7105 7137 7179 7211 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 1456 1106 878 561 347 334 187 146 135 98 69 42 32 32 32 32 32 32 32 32 32 32 32 32 32 32 31 HincII GTy'rAC Cuts at: 669 1657 1938 2673 4471 5808 6054 669 Size: 988 281 735 1798 1337 246 3079 Fragments arranged by size: 3079 1798 1337 988 735 281 246 HindIII A'AGCT_T Cuts at: 748 3684 4016 748 Size: 2936 332 5196 Fragments arranged by size: 5196 2936 332 HpaI GTT'AAC Cuts at: 2673 4471 6054 2673 Size: 1798 1583 5083 Fragments arranged by size: 5083 1798 1583 KpnI G_GTAC'C Cuts at: 5789 5789 Size: 8464 MfeI C'AATT_G Cuts at: 4087 6063 4087 Size: 1976 6488 MluI A'CGCG_T Cuts at: 8340 8340 Size: 8464 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2351 2492 3849 4128 7578 7762 1305 Size: 1046 141 1357 279 3450 184 2007 Fragments arranged by size: 3450 2007 1357 1046 279 184 141 MscI TGG'CCA Cuts at: 10 64 1489 1832 2537 4844 5394 10 Size: 54 1425 343 705 2307 550 3080 Fragments arranged by size: 3080 2307 1425 705 550 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2481 4604 5459 6422 6781 6940 518 Size: 648 1315 2123 855 963 359 159 2042 Fragments arranged by size: 2123 2042 1315 963 855 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2803 4081 4604 4806 5880 6557 7705 7950 Size: 1197 1278 523 202 1074 677 1148 245 Cuts at: 7950 1606 Size: 2120 Fragments arranged by size: 2120 1278 1197 1148 1074 677 523 245 202 NcoI C'CATG_G Cuts at: 513 1065 2004 4839 513 Size: 552 939 2835 4138 Fragments arranged by size: 4138 2835 939 552 NdeI CA'TA_TG Cuts at: 387 3079 387 Size: 2692 5772 NheI G'CTAG_C Cuts at: 1052 1052 Size: 8464 NotI GC'GGCC_GC Cuts at: 5911 5911 Size: 8464 NspI r_CATG'y Cuts at: 1132 1229 2608 5822 7367 1132 Size: 97 1379 3214 1545 2229 Fragments arranged by size: 3214 2229 1545 1379 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 8464 PciI A'CATG_T Cuts at: 7363 7363 Size: 8464 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 8464 PfoI T'CCnGG_A Cuts at: 5232 5232 Size: 8464 PmeI GTTT'AAAC Cuts at: 5768 5768 Size: 8464 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 6533 6565 8046 8078 6533 Size: 32 1481 32 6919 Fragments arranged by size: 6919 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 4089 1613 Size: 2476 5988 PsiI TTA'TAA Cuts at: 4881 4929 6034 4881 Size: 48 1105 7311 Fragments arranged by size: 7311 1105 48 PspXI vC'TCGA_Gb Cuts at: 3806 3806 Size: 8464 PstI C_TGCA'G Cuts at: 830 3465 5816 830 Size: 2635 2351 3478 Fragments arranged by size: 3478 2635 2351 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 8464 PvuII CAG'CTG Cuts at: 2803 4081 4604 4806 2803 Size: 1278 523 202 6461 Fragments arranged by size: 6461 1278 523 202 SacI G_AGCT'C Cuts at: 2930 2930 Size: 8464 SalI G'TCGA_C Cuts at: 1936 5806 1936 Size: 3870 4594 SapI GCTCTTCn'nnn_ Cuts at: 2004 2371 2004 Size: 367 8097 SbfI CC_TGCA'GG Cuts at: 5816 5816 Size: 8464 ScaI AGT'ACT Cuts at: 2726 6712 8424 2726 Size: 3986 1712 2766 Fragments arranged by size: 3986 2766 1712 SfcI C'TryA_G Cuts at: 826 1045 2758 3461 5812 6947 7628 7819 826 Size: 219 1713 703 2351 1135 681 191 1471 Fragments arranged by size: 2351 1713 1471 1135 703 681 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 8464 SgrAI Cr'CCGG_yG Cuts at: 1700 3291 1700 Size: 1591 6873 SmaI CCC'GGG Cuts at: 1872 5791 1872 Size: 3919 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2422 2461 3452 3806 3815 5609 Size: 197 926 479 39 991 354 9 1794 Cuts at: 5609 6568 7469 7731 820 Size: 959 901 262 1553 Fragments arranged by size: 1794 1553 991 959 926 901 479 354 262 197 39 9 SnaBI TAC'GTA Cuts at: 493 6255 493 Size: 5762 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 8464 SphI G_CATG'C Cuts at: 1132 2608 5822 1132 Size: 1476 3214 3774 Fragments arranged by size: 3774 3214 1476 SspI AAT'ATT Cuts at: 5 52 2311 5098 5401 6388 5 Size: 47 2259 2787 303 987 2081 Fragments arranged by size: 2787 2259 2081 987 303 47 StuI AGG'CCT Cuts at: 1517 3206 4680 5003 1517 Size: 1689 1474 323 4978 Fragments arranged by size: 4978 1689 1474 323 StyI C'CwwG_G Cuts at: 513 1065 2004 4002 4789 4839 5903 513 Size: 552 939 1998 787 50 1064 3074 Fragments arranged by size: 3074 1998 1064 939 787 552 50 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 5453 6491 6508 6661 6846 7291 325 Size: 1255 3873 1038 17 153 185 445 1498 Fragments arranged by size: 3873 1498 1255 1038 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2724 2781 6710 Size: 274 80 33 51 1443 747 57 3929 Cuts at: 6710 8422 96 Size: 1712 138 Fragments arranged by size: 3929 1712 1443 747 274 138 80 57 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3637 3669 4546 4578 8424 8456 3637 Size: 32 877 32 3846 32 3645 Fragments arranged by size: 3846 3645 877 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 2483 3348 1316 Size: 1167 865 6432 Fragments arranged by size: 6432 1167 865 XbaI T'CTAG_A Cuts at: 4210 4283 5800 4210 Size: 73 1517 6874 Fragments arranged by size: 6874 1517 73 XcmI CCAnnnn_n'nnnnTGG Cuts at: 3010 4606 3010 Size: 1596 6868 XhoI C'TCGA_G Cuts at: 1943 3806 1943 Size: 1863 6601 XmaI C'CCGG_G Cuts at: 1870 5789 1870 Size: 3919 4545 XmnI GAAnn'nnTTC Cuts at: 2218 4967 6593 2218 Size: 2749 1626 4089 Fragments arranged by size: 4089 2749 1626 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 6272 276 Size: 53 83 186 936 4738 2468 Fragments arranged by size: 4738 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaLI ApoI AseI AsiSI AvaI AvrII BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI EcoRV FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PshAI PsiI PspXI PstI PvuI PvuII SacI SalI SapI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI ApaI AscI BaeI BbeI BfrBI BsiWI BsmBI BstXI BstZ17I DraIII FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PmlI PpuMI PspOMI PsrI RsrII SacII SanDI SexAI SfiI SfoI SrfI SwaI