Restriction map for
pFN21AB5625
(Linear) (Six-base) MAPSORT of: KIBB5625.seq Check: 9839 from: 1 to: 3759
KIBB5625.orf
With 182 enzymes: SgfI *
February 28, 2007 11:27 ..
AccI GT'mk_AC
Cuts at: 0 482 2951 3759
Size: 482 2469 808
Fragments arranged by size:
2469 808 482
AclI AA'CG_TT
Cuts at: 0 3486 3759
Size: 3486 273
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 977 2029 2143 3759
Size: 977 1052 114 1616
Fragments arranged by size:
1616 1052 977 114
AflII C'TTAA_G
Cuts at: 0 1810 3759
Size: 1810 1949
AleI CACnn'nnGTG
Cuts at: 0 2599 3759
Size: 2599 1160
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 3152 3759
Size: 3152 607
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 531 563 3759
Size: 531 32 3196
Fragments arranged by size:
3196 531 32
AlwNI CAG_nnn'CTG
Cuts at: 0 1347 1367 2241 2938 3759
Size: 1347 20 874 697 821
Fragments arranged by size:
1347 874 821 697 20
ApoI r'AATT_y
Cuts at: 0 1165 2291 2917 3759
Size: 1165 1126 626 842
Fragments arranged by size:
1165 1126 842 626
AseI AT'TA_AT
Cuts at: 0 2510 3759
Size: 2510 1249
AvaI C'yCGr_G
Cuts at: 0 1089 1801 3759
Size: 1089 712 1958
Fragments arranged by size:
1958 1089 712
AvrII C'CTAG_G
Cuts at: 0 2784 3759
Size: 2784 975
BanI G'GyrC_C
Cuts at: 0 1520 3759
Size: 1520 2239
BanII G_rGCy'C
Cuts at: 0 925 1504 3198 3759
Size: 925 579 1694 561
Fragments arranged by size:
1694 925 579 561
BbsI GAAGACnn'nnnn_
Cuts at: 0 65 2074 2174 3359 3436 3759
Size: 65 2009 100 1185 77 323
Fragments arranged by size:
2009 1185 323 100 77 65
BbvCI CC'TCA_GC
Cuts at: 0 112 3759
Size: 112 3647
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 503 537 3759
Size: 503 34 3222
Fragments arranged by size:
3222 503 34
BciVI GTATCCnnnnn_n'
Cuts at: 0 1717 3759
Size: 1717 2042
BclI T'GATC_A
Cuts at: 0 384 1062 1836 3597 3759
Size: 384 678 774 1761 162
Fragments arranged by size:
1761 774 678 384 162
BglII A'GATC_T
Cuts at: 0 1747 3759
Size: 1747 2012
Bme1580I G_kGCm'C
Cuts at: 0 3312 3759
Size: 3312 447
BmgBI CAC'GTC
Cuts at: 0 1264 3759
Size: 1264 2495
BmrI ACTGGGnnnn_n'
Cuts at: 0 1415 3759
Size: 1415 2344
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 78 2073 3463 3759
Size: 78 1995 1390 296
Fragments arranged by size:
1995 1390 296 78
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 708 1130 1369 2194 2418 3759
Size: 708 422 239 825 224 1341
Fragments arranged by size:
1341 825 708 422 239 224
Bpu10I CC'TnA_GC
Cuts at: 0 112 252 924 3759
Size: 112 140 672 2835
Fragments arranged by size:
2835 672 140 112
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 438 441 1468 3589 3759
Size: 438 3 1027 2121 170
Fragments arranged by size:
2121 1027 438 170 3
BsaI GGTCTCn'nnnn_
Cuts at: 0 1345 3031 3759
Size: 1345 1686 728
Fragments arranged by size:
1686 1345 728
BsaHI Gr'CG_yC
Cuts at: 0 3216 3759
Size: 3216 543
BsaWI w'CCGG_w
Cuts at: 0 1526 1952 3344 3759
Size: 1526 426 1392 415
Fragments arranged by size:
1526 1392 426 415
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 1465 1495 1560 1590 1632 1662 3759
Size: 1465 30 65 30 42 30 2097
Fragments arranged by size:
2097 1465 65 42 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 1029 1486 1640 3759
Size: 1029 457 154 2119
Fragments arranged by size:
2119 1029 457 154
BseYI C'CCAG_C
Cuts at: 0 1214 2072 3759
Size: 1214 858 1687
Fragments arranged by size:
1687 1214 858
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1447 3759
Size: 1447 2312
BsiHKAI G_wGCw'C
Cuts at: 0 925 1612 1881 2633 2816 3759
Size: 925 687 269 752 183 943
Fragments arranged by size:
943 925 752 687 269 183
BsmI GAATG_Cn'
Cuts at: 0 72 635 3615 3759
Size: 72 563 2980 144
Fragments arranged by size:
2980 563 144 72
Bsp1286I G_dGCh'C
Cuts at: 0 925 1504 1612 1881 2633 2816 3198 3312
Size: 925 579 108 269 752 183 382 114
Cuts at: 3312 3759
Size: 447
Fragments arranged by size:
925 752 579 447 382 269 183 114
108
BspEI T'CCGG_A
Cuts at: 0 3344 3759
Size: 3344 415
BspHI T'CATG_A
Cuts at: 0 1496 3657 3759
Size: 1496 2161 102
Fragments arranged by size:
2161 1496 102
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 3344 3442 3759
Size: 3344 98 317
Fragments arranged by size:
3344 317 98
BsrDI GCAATG_nn'
Cuts at: 0 1772 3759
Size: 1772 1987
BsrFI r'CCGG_y
Cuts at: 0 1286 3759
Size: 1286 2473
BsrGI T'GTAC_A
Cuts at: 0 776 3759
Size: 776 2983
BssSI C'ACGA_G
Cuts at: 0 1004 3759
Size: 1004 2755
BstYI r'GATC_y
Cuts at: 0 867 1156 1747 3704 3759
Size: 867 289 591 1957 55
Fragments arranged by size:
1957 867 591 289 55
Bsu36I CC'TnA_GG
Cuts at: 0 1203 3759
Size: 1203 2556
BtgI C'CryG_G
Cuts at: 0 1415 2834 3759
Size: 1415 1419 925
Fragments arranged by size:
1419 1415 925
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 3671 3759
Size: 3671 88
BtsI GCAGTG_nn'
Cuts at: 0 1590 2745 3759
Size: 1590 1155 1014
Fragments arranged by size:
1590 1155 1014
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 3441 3476 3759
Size: 3441 35 283
Fragments arranged by size:
3441 283 35
DraI TTT'AAA
Cuts at: 0 147 2480 3759
Size: 147 2333 1279
Fragments arranged by size:
2333 1279 147
EaeI y'GGCC_r
Cuts at: 0 396 530 2837 3387 3759
Size: 396 134 2307 550 372
Fragments arranged by size:
2307 550 396 372 134
EarI CTCTTCn'nnn_
Cuts at: 0 135 366 2119 3759
Size: 135 231 1753 1640
Fragments arranged by size:
1753 1640 231 135
EcoICRI GAG'CTC
Cuts at: 0 923 3759
Size: 923 2836
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 708 977 1130 1369 2029 2143 2194 2418
Size: 708 269 153 239 660 114 51 224
Cuts at: 2418 3759
Size: 1341
Fragments arranged by size:
1341 708 660 269 239 224 153 114
51
EcoNI CCTnn'n_nnAGG
Cuts at: 0 1195 3759
Size: 1195 2564
EcoO109I rG'GnC_Cy
Cuts at: 0 413 3759
Size: 413 3346
EcoRV GAT'ATC
Cuts at: 0 361 3759
Size: 361 3398
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 620 2241 3372 3759
Size: 620 1621 1131 387
Fragments arranged by size:
1621 1131 620 387
HaeII r_GCGC'y
Cuts at: 0 655 3759
Size: 655 3104
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 673 705 1266 1298 1329 1361 1459 1491
Size: 673 32 561 32 31 32 98 32
Cuts at: 1491 1560 1592 1926 1958 2836 2868 3055 3087
Size: 69 32 334 32 878 32 187 32
Cuts at: 3087 3434 3466 3612 3644 3759
Size: 347 32 146 32 115
Fragments arranged by size:
878 673 561 347 334 187 146 115
98 69 32 32 32 32 32 32
32 32 32 32 31
HincII GTy'rAC
Cuts at: 0 668 2466 3759
Size: 668 1798 1293
Fragments arranged by size:
1798 1293 668
HindIII A'AGCT_T
Cuts at: 0 1679 2011 3759
Size: 1679 332 1748
Fragments arranged by size:
1748 1679 332
HpaI GTT'AAC
Cuts at: 0 668 2466 3759
Size: 668 1798 1293
Fragments arranged by size:
1798 1293 668
MfeI C'AATT_G
Cuts at: 0 2082 3759
Size: 2082 1677
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 346 487 1844 2123 3759
Size: 346 141 1357 279 1636
Fragments arranged by size:
1636 1357 346 279 141
MscI TGG'CCA
Cuts at: 0 532 2839 3389 3759
Size: 532 2307 550 370
Fragments arranged by size:
2307 550 532 370
MslI CAynn'nnrTG
Cuts at: 0 476 2599 3454 3759
Size: 476 2123 855 305
Fragments arranged by size:
2123 855 476 305
MspA1I CmG'CkG
Cuts at: 0 798 2076 2599 2801 3759
Size: 798 1278 523 202 958
Fragments arranged by size:
1278 958 798 523 202
NcoI C'CATG_G
Cuts at: 0 2834 3759
Size: 2834 925
NdeI CA'TA_TG
Cuts at: 0 1074 3759
Size: 1074 2685
NspI r_CATG'y
Cuts at: 0 603 3759
Size: 603 3156
PfoI T'CCnGG_A
Cuts at: 0 3227 3759
Size: 3227 532
PshAI GACnn'nnGTC
Cuts at: 0 2084 3759
Size: 2084 1675
PsiI TTA'TAA
Cuts at: 0 2876 2924 3759
Size: 2876 48 835
Fragments arranged by size:
2876 835 48
PspXI vC'TCGA_Gb
Cuts at: 0 1801 3759
Size: 1801 1958
PstI C_TGCA'G
Cuts at: 0 1460 3759
Size: 1460 2299
PvuII CAG'CTG
Cuts at: 0 798 2076 2599 2801 3759
Size: 798 1278 523 202 958
Fragments arranged by size:
1278 958 798 523 202
SacI G_AGCT'C
Cuts at: 0 925 3759
Size: 925 2834
SapI GCTCTTCn'nnn_
Cuts at: 0 366 3759
Size: 366 3393
ScaI AGT'ACT
Cuts at: 0 721 3759
Size: 721 3038
SfcI C'TryA_G
Cuts at: 0 753 1456 3759
Size: 753 703 2303
Fragments arranged by size:
2303 753 703
SgrAI Cr'CCGG_yG
Cuts at: 0 1286 3759
Size: 1286 2473
SmlI C'TyrA_G
Cuts at: 0 417 456 1447 1801 1810 3604 3759
Size: 417 39 991 354 9 1794 155
Fragments arranged by size:
1794 991 417 354 155 39 9
SphI G_CATG'C
Cuts at: 0 603 3759
Size: 603 3156
SspI AAT'ATT
Cuts at: 0 306 3093 3396 3759
Size: 306 2787 303 363
Fragments arranged by size:
2787 363 306 303
StuI AGG'CCT
Cuts at: 0 1201 2675 2998 3759
Size: 1201 1474 323 761
Fragments arranged by size:
1474 1201 761 323
StyI C'CwwG_G
Cuts at: 0 1997 2784 2834 3759
Size: 1997 787 50 925
Fragments arranged by size:
1997 925 787 50
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 3448 3759
Size: 3448 311
TatI w'GTAC_w
Cuts at: 0 719 776 3759
Size: 719 57 2983
Fragments arranged by size:
2983 719 57
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1632 1664 2541 2573 3759
Size: 1632 32 877 32 1186
Fragments arranged by size:
1632 1186 877 32 32
Tth111I GACn'n_nGTC
Cuts at: 0 478 1343 3759
Size: 478 865 2416
Fragments arranged by size:
2416 865 478
XbaI T'CTAG_A
Cuts at: 0 2205 2278 3759
Size: 2205 73 1481
Fragments arranged by size:
2205 1481 73
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 1005 2601 3759
Size: 1005 1596 1158
Fragments arranged by size:
1596 1158 1005
XhoI C'TCGA_G
Cuts at: 0 1801 3759
Size: 1801 1958
XmnI GAAnn'nnTTC
Cuts at: 0 213 2962 3759
Size: 213 2749 797
Fragments arranged by size:
2749 797 213
Enzymes that do cut:
AccI AclI AcuI AflII AleI AlfI AloI AlwNI
ApoI AseI AvaI AvrII BanI BanII BbsI BbvCI
BcgI BciVI BclI BglII Bme1580I BmgBI BmrI BplI
BpmI Bpu10I BpuEI BsaI BsaHI BsaWI BsaXI BseRI
BseYI BsgI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI
BsrDI BsrFI BsrGI BssSI BstYI Bsu36I BtgI BtgZI
BtsI CspCI DraI EaeI EarI EcoICRI Eco57MI EcoNI
EcoO109I EcoRV FalI HaeII Hin4I HincII HindIII HpaI
MfeI MmeI MscI MslI MspA1I NcoI NdeI NspI
PfoI PshAI PsiI PspXI PstI PvuII SacI SapI
ScaI SfcI SgrAI SmlI SphI SspI StuI StyI
TaqII TatI TstI Tth111I XbaI XcmI XhoI XmnI
Enzymes that do not cut:
AarI AatII Acc65I AfeI AflIII AgeI AhdI ApaI
ApaLI AscI AsiSI BaeI BamHI BbeI BfrBI BglI
BlpI BmtI BsaAI BsaBI BsiEI BsiWI BsmBI BsrBI
BssHII BstAPI BstBI BstEII BstXI BstZ17I ClaI DraIII
DrdI EagI EciI EcoRI FseI FspI FspAI KasI
KpnI MluI NaeI NarI NgoMIV NheI NotI NruI
NsiI PacI PasI PciI PflMI PmeI PmlI PpiI
PpuMI PspOMI PsrI PvuI RsrII SacII SalI SanDI
SbfI SexAI SfiI SfoI SgfI SmaI SnaBI SpeI
SrfI SwaI XmaI ZraI