Restriction map for
pFN21AB5613
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# NO CUT ENZYMES ( 121 enzymes) #
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AatII Acc65I AccI AccIII AclI
AflII AflIII AgeI AhdI Alw44I
Aor51HI ApaI ApaLI AscI Asp718I
AspEI AspI AviII AvrII BanII
BbeI BbrPI BbsI BciVI BclI
BfaI BfrI BglII BlnI BlpI
Bpu1102I BpuAI BsaAI BsaWI BseAI
BsiEI BsiWI BsmI Bsp1407I BspDI
BspEI BspHI BspLU11I BsrDI BsrFI
BsrGI BssHII BssSI Bst1107I BstBI
BstXI BstZ17I CelII Cfr10I ClaI
CpoI DraIII DrdI EagI Eam1105I
EclXI Eco47III Eco52I EcoNI EcoRI
FauI FbaI FseI FspI HindIII
KasI KpnI KspI MaeI MaeII
MfeI MluI MroI MslI MunI
NaeI NarI NgoMIV NheI NotI
NruI NspV PacI PflFI PflMI
PinAI PmaCI PmeI PmlI Psp1406I
PvuI RcaI RsrII SacI SacII
SalI ScaI SexAI SfiI SfoI
SfuI SgrAI SmaI SnaBI SpeI
SphI SspBI SspI StuI SwaI
Tth111I Van91I XbaI XcmI XmaCI
XmaI
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# ONE CUT ENZYMES ( 71 enzymes) #
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AccII 698 CGCG CG^CG
AcyI 667 GACGCC G[GA]^CG[CT]C
AocI 196 CCTAAGG CC^T[ACGT]AGG
AseI 874 ATTAAT AT^TAAT
AsnI 874 ATTAAT AT^TAAT
Asp700I 680 GAAGAGATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspHI 205 GTGCTC G[AT]GC[AT]^C
AvaI 1037 CTCGAG C^[CT]CG[GA]G
BalI 821 TGGCCA TGG^CCA
BamHI 566 GGATCC G^GATCC
BbvCI 880 GCTGAGG CCTCAGC(-5/-2)
BcgI 1231 CGATACAAATGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BcnI 563 CCCGG CC^[GC]GG
BglI 93 GCCACCTGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BmyI 205 GTGCTC G[AGT]GC[ACT]^C
BsaBI 1246 GATATGCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaHI 667 GACGCC G[GA]^CG[CT]C
BsaI 1148 GAGACC GGTCTC(1/5)
BsgI 112 GTGCAG GTGCAG(16/14)
BsiHKAI 205 GTGCTC G[AT]GC[AT]^C
BsmBI 1034 CGTCTC CGTCTC(1/5)
BsoBI 1037 CTCGAG C^[CT]CG[GA]G
Bsp1286I 205 GTGCTC G[AGT]GC[ACT]^C
BspMI 44 ACCTGC ACCTGC(4/8)
BsrBI 925 GAGCGG CCGCTC(-3/-3)
BstAPI 409 GCAGCTACTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstF5I 1278 CATCC GGATG(2/0)
BstUI 698 CGCG CG^CG
BstYI 566 GGATCC [GA]^GATC[CT]
Bsu36I 196 CCTAAGG CC^T[ACGT]AGG
CfoI 560 GCGC GCG^C
DraI 661 TTTAAA TTT^AAA
DraII 260 GGGTCCT [GA]G^G[ACGT]CC[CT]
DsaI 818 CCATGG C^C[GA][CT]GG
EaeI 821 TGGCCA [CT]^GGCC[GA]
Eco81I 196 CCTAAGG CC^T[ACGT]AGG
EcoO109I 260 GGGTCCT [GA]G^G[ACGT]CC[CT]
EcoRV 423 GATATC GAT^ATC
EcoT14I 818 CCATGG C^C[AT][AT]GG
FokI 1278 CATCC GGATG(9/13)
HaeII 559 AGCGCC [GA]GCGC^[CT]
HapII 564 CCGG C^CGG
HhaI 560 GCGC GCG^C
Hin1I 667 GACGCC G[GA]^CG[CT]C
HinP1I 560 GCGC G^CGC
HincII 58 GTTAAC GT[CT]^[GA]AC
HindII 58 GTTAAC GT[CT]^[GA]AC
HpaI 58 GTTAAC GTT^AAC
HpaII 564 CCGG C^CGG
MamI 1246 GATATGCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MflI 566 GGATCC [GA]^GATC[CT]
MluNI 821 TGGCCA TGG^CCA
MscI 821 TGGCCA TGG^CCA
MspI 564 CCGG C^CGG
MvnI 698 CGCG CG^CG
NciI 563 CCCGG CC^[GC]GG
NcoI 818 CCATGG C^CATGG
NdeI 734 CATATG CA^TATG
PaeR7I 1037 CTCGAG C^TCGAG
PpuMI 260 GGGTCCT [GA]G^G[AT]CC[CT]
PshAI 1374 GACTGGAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PshBI 874 ATTAAT AT^TAAT
SapI 1137 GCTCTTC GCTCTTC(1/4)
SbfI 45 CCTGCAGG CCTGCA^GG
SfaNI 1251 GCATC GCATC(5/9)
SmlI 1037 CTCGAG C^T[CT][GA]AG
Sse8387I 45 CCTGCAGG CCTGCA^GG
StyI 818 CCATGG C^C[AT][AT]GG
XhoI 1037 CTCGAG C^TCGAG
XhoII 566 GGATCC [GA]^GATC[CT]
XmnI 680 GAAGAGATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 14 enzymes) #
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AcsI 939 AAATTT [GA]^AATT[CT]
1167 GAATTT
AlwI 567 GATCC GGATC(4/5)
566 GGATC
AlwNI 410 CAGCTACTG CAG[ACGT][ACGT][ACGT]^CTG
1208 CAGTTGCTG
ApoI 939 AAATTT [GA]^AATT[CT]
1167 GAATTT
BanI 163 GGCACC G^G[CT][GA]CC
1266 GGTGCC
BstEII 40 GGTTACC G^GT[ACGT]ACC
1045 GGTCACC
BstPI 40 GGTTACC G^GT[ACGT]ACC
1045 GGTCACC
EcoO65I 40 GGTTACC G^GT[ACGT]ACC
1045 GGTCACC
EcoT22I 731 ATGCAT ATGCA^T
1249 ATGCAT
HaeIII 822 GGCC GG^CC
858 GGCC
HphI 830 TCACC GGTGA(8/7)
1047 TCACC
NsiI 731 ATGCAT ATGCA^T
1249 ATGCAT
NspI 230 GCATGT [GA]CATG^[CT]
1300 ACATGC
PstI 46 CTGCAG CTGCA^G
407 CTGCAG
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# 3 OR MORE CUT ENZYMES ( 52 enzymes) #
#########################################
AciI AfaI AluI AvaII BbvI
BpmI BsaJI BseRI BsiYI BslI
BsmAI BsmFI BsrI BssKI BstNI
Cac8I Cfr13I DdeI DpnII EarI
EcoRII Fnu4HI HgaI HinfI ItaI
Ksp632I MaeIII MboI MboII MnlI
MseI MspA1I MvaI MwoI NdeII
NlaIII NlaIV PleI PvuII RsaI
Sau3AI Sau96I ScrFI SfcI TaqI
TfiI Tru9I TseI Tsp45I Tsp509I
TspRI TthHB8I