(Circular) (Six-base) MAPSORT of: pFN21AB5613.seq Check: 657 from: 1 to: 6208 pFN21AB5613 6208 bp With 182 enzymes: SgfI * February 27, 2009 14:34 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4018 278 Size: 53 83 186 936 2482 2468 Fragments arranged by size: 2482 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3551 1937 Size: 1614 4594 Acc65I G'GTAC_C Cuts at: 3529 3529 Size: 6208 AclI AA'CG_TT Cuts at: 1563 4335 4708 1563 Size: 2772 373 3063 Fragments arranged by size: 3063 2772 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2144 2793 4271 5655 5923 1632 Size: 512 649 1478 1384 268 1917 Fragments arranged by size: 1917 1478 1384 649 512 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6011 AflIII A'CryG_T Cuts at: 1203 5107 6084 1203 Size: 3904 977 1327 Fragments arranged by size: 3904 1327 977 AgeI A'CCGG_T Cuts at: 5039 5039 Size: 6208 AhdI GACnn_n'nnGTC Cuts at: 4937 4937 Size: 6208 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6208 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6208 AlwNI CAG_nnn'CTG Cuts at: 2420 3218 3951 5523 2420 Size: 798 733 1572 3105 Fragments arranged by size: 3105 1572 798 733 ApaLI G'TGCA_C Cuts at: 4265 5421 4265 Size: 1156 5052 ApoI r'AATT_y Cuts at: 1493 2944 3172 3517 3743 3895 6004 1493 Size: 1451 228 345 226 152 2109 1697 Fragments arranged by size: 2109 1697 1451 345 228 226 152 AseI AT'TA_AT Cuts at: 160 1032 2880 4762 160 Size: 872 1848 1882 1606 Fragments arranged by size: 1882 1848 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6208 AvaI C'yCGr_G Cuts at: 1870 1943 3042 3533 1870 Size: 73 1099 491 4545 Fragments arranged by size: 4545 1099 491 73 BamHI G'GATC_C Cuts at: 2571 3538 3905 2571 Size: 967 367 4874 Fragments arranged by size: 4874 967 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2168 3271 3529 4984 618 Size: 325 217 627 381 1103 258 1455 1842 Fragments arranged by size: 1842 1455 1103 627 381 325 258 217 BanII G_rGCy'C Cuts at: 1391 3527 1391 Size: 2136 4072 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 5237 BbvCI CC'TCA_GC Cuts at: 2886 2886 Size: 6208 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3225 3259 4399 4433 1903 Size: 34 1288 34 1140 34 3678 Fragments arranged by size: 3678 1288 1140 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4100 5310 4100 Size: 1210 4998 BclI T'GATC_A Cuts at: 1548 1548 Size: 6208 BfrBI ATG'CAT Cuts at: 2738 3256 2738 Size: 518 5690 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2104 4819 136 Size: 107 122 71 631 605 432 2715 1525 Fragments arranged by size: 2715 1525 631 605 432 122 107 71 BglII A'GATC_T Cuts at: 6203 6203 Size: 6208 BlpI GC'TnA_GC Cuts at: 3625 3625 Size: 6208 Bme1580I G_kGCm'C Cuts at: 1790 4269 5425 1790 Size: 2479 1156 2573 Fragments arranged by size: 2573 2479 1156 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6208 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2948 3939 4897 Size: 416 53 83 399 24 1531 991 958 Cuts at: 4897 442 Size: 1753 Fragments arranged by size: 1753 1531 991 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6208 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2198 2346 2581 2649 3004 3171 3402 4868 Size: 959 148 235 68 355 167 231 1466 Cuts at: 4868 1239 Size: 2579 Fragments arranged by size: 2579 1466 959 355 235 231 167 148 68 Bpu10I CC'TnA_GC Cuts at: 2886 2886 Size: 6208 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4333 5198 5496 4333 Size: 865 298 5045 Fragments arranged by size: 5045 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 3147 4871 882 Size: 617 1648 1724 2219 Fragments arranged by size: 2219 1724 1648 617 BsaAI yAC'GTr Cuts at: 493 1204 3999 493 Size: 711 2795 2702 Fragments arranged by size: 2795 2702 711 BsaBI GATnn'nnATC Cuts at: 3255 6202 3255 Size: 2947 3261 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2673 4015 4397 5032 Size: 53 83 186 936 1140 1342 382 635 Cuts at: 5032 275 Size: 1451 Fragments arranged by size: 1451 1342 1140 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4641 5039 5313 5460 5871 4641 Size: 398 274 147 411 4978 Fragments arranged by size: 4978 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5983 Fragments arranged by size: 5983 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2387 2903 3087 1186 Size: 1201 516 184 4307 Fragments arranged by size: 4307 1201 516 184 BseYI C'CCAG_C Cuts at: 3218 3286 5411 5907 3218 Size: 68 2125 496 3519 Fragments arranged by size: 3519 2125 496 68 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2138 1163 Size: 573 402 5233 Fragments arranged by size: 5233 573 402 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3658 4419 5447 1540 Size: 275 186 1657 761 1028 2301 Fragments arranged by size: 2301 1657 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2214 4269 4354 5425 2214 Size: 2055 85 1071 2997 Fragments arranged by size: 2997 2055 1071 85 BsmI GAATG_Cn' Cuts at: 3719 3812 3719 Size: 93 6115 BsmBI CGTCTCn'nnnn_ Cuts at: 3045 3045 Size: 6208 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2214 3527 4269 4354 5425 1391 Size: 399 424 1313 742 85 1071 2174 Fragments arranged by size: 2174 1313 1071 742 424 399 85 BspEI T'CCGG_A Cuts at: 5871 5871 Size: 6208 BspHI T'CATG_A Cuts at: 4097 5827 4097 Size: 1730 4478 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2058 3563 844 Size: 1214 1505 3489 Fragments arranged by size: 3489 1505 1214 BsrBI CCG'CTC Cuts at: 1394 1823 2932 4095 1394 Size: 429 1109 1163 3507 Fragments arranged by size: 3507 1163 1109 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4703 4877 65 Size: 1183 3455 174 1396 Fragments arranged by size: 3455 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4852 5039 1700 Size: 110 3042 187 2869 Fragments arranged by size: 3042 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6208 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6208 BssSI C'ACGA_G Cuts at: 4268 5280 4268 Size: 1012 5196 BstAPI GCAn_nnn'nTGC Cuts at: 794 2420 794 Size: 1626 4582 BstBI TT'CG_AA Cuts at: 5012 5012 Size: 6208 BstEII G'GTnAC_C Cuts at: 1184 2045 3050 1184 Size: 861 1005 4342 Fragments arranged by size: 4342 1005 861 BstYI r'GATC_y Cuts at: 1972 2571 3538 3905 4290 4307 5759 5845 5857 Size: 599 967 367 385 17 1452 86 12 Cuts at: 5857 6203 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 967 599 385 367 346 86 17 12 Bsu36I CC'TnA_GG Cuts at: 2202 2202 Size: 6208 BtgI C'CryG_G Cuts at: 513 1065 2004 2823 513 Size: 552 939 819 3898 Fragments arranged by size: 3898 939 819 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5469 Fragments arranged by size: 5469 683 56 BtsI GCAGTG_nn' Cuts at: 2916 2922 3724 4518 4538 2916 Size: 6 802 794 20 4586 Fragments arranged by size: 4586 802 794 20 6 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6208 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 2892 2927 529 Size: 35 2328 35 3810 Fragments arranged by size: 3810 2328 35 35 DraI TTT'AAA Cuts at: 2668 3512 2668 Size: 844 5364 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5215 809 Size: 507 3899 1802 Fragments arranged by size: 3899 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2826 3655 4544 Size: 54 1425 50 275 18 996 829 889 Cuts at: 4544 6173 8 Size: 1629 43 Fragments arranged by size: 1629 1425 996 889 829 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3655 1537 Size: 275 1843 4090 Fragments arranged by size: 4090 1843 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2120 2680 3149 3317 4138 1353 Size: 767 560 469 168 821 3423 Fragments arranged by size: 3423 821 767 560 469 168 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4795 5169 5315 387 Size: 1064 138 3206 374 146 1280 Fragments arranged by size: 3206 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2144 2198 2346 2581 2649 2793 3004 Size: 393 512 54 148 235 68 144 211 Cuts at: 3004 3171 3402 4271 4868 5655 5923 1239 Size: 167 231 869 597 787 268 1524 Fragments arranged by size: 1524 869 787 597 512 393 268 235 231 211 167 148 144 68 54 EcoO109I rG'GnC_Cy Cuts at: 2266 2266 Size: 6208 EcoRI G'AATT_C Cuts at: 3517 3895 3517 Size: 378 5830 EcoRV GAT'ATC Cuts at: 2430 2430 Size: 6208 FspI TGC'GCA Cuts at: 4714 4714 Size: 6208 HaeII r_GCGC'y Cuts at: 1678 2568 5355 1678 Size: 890 2787 2531 Fragments arranged by size: 2787 2531 890 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2172 2204 4849 4881 4923 Size: 32 135 32 600 32 2645 32 42 Cuts at: 4923 4955 1373 Size: 32 2626 Fragments arranged by size: 2645 2626 600 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2065 3552 3798 669 Size: 988 281 127 1487 246 3079 Fragments arranged by size: 3079 1487 988 281 246 127 HindIII A'AGCT_T Cuts at: 748 748 Size: 6208 HpaI GTT'AAC Cuts at: 2065 3798 2065 Size: 1733 4475 KpnI G_GTAC'C Cuts at: 3533 3533 Size: 6208 MfeI C'AATT_G Cuts at: 3807 3807 Size: 6208 MluI A'CGCG_T Cuts at: 6084 6084 Size: 6208 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 5322 5506 1305 Size: 4017 184 2007 Fragments arranged by size: 4017 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2828 10 Size: 54 1425 343 996 3390 Fragments arranged by size: 3390 1425 996 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 4166 4525 4684 518 Size: 648 3000 359 159 2042 Fragments arranged by size: 3000 2042 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2122 2513 2559 3107 3624 4301 5449 5694 Size: 516 391 46 548 517 677 1148 245 Cuts at: 5694 1606 Size: 2120 Fragments arranged by size: 2120 1148 677 548 517 516 391 245 46 NcoI C'CATG_G Cuts at: 513 1065 2004 2823 513 Size: 552 939 819 3898 Fragments arranged by size: 3898 939 819 552 NdeI CA'TA_TG Cuts at: 387 2740 387 Size: 2353 3855 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6208 NotI GC'GGCC_GC Cuts at: 3655 3655 Size: 6208 NsiI A_TGCA'T Cuts at: 2740 3258 2740 Size: 518 5690 NspI r_CATG'y Cuts at: 1132 1229 2239 3309 3566 5111 1132 Size: 97 1010 1070 257 1545 2229 Fragments arranged by size: 2229 1545 1070 1010 257 97 PasI CC'CwG_GG Cuts at: 1348 2083 1348 Size: 735 5473 PciI A'CATG_T Cuts at: 5107 5107 Size: 6208 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 6208 PmeI GTTT'AAAC Cuts at: 3512 3512 Size: 6208 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4277 4309 5790 5822 4277 Size: 32 1481 32 4663 Fragments arranged by size: 4663 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2266 2266 Size: 6208 PshAI GACnn'nnGTC Cuts at: 1613 3383 1613 Size: 1770 4438 PsiI TTA'TAA Cuts at: 3778 3778 Size: 6208 PstI C_TGCA'G Cuts at: 830 2055 2416 3560 830 Size: 1225 361 1144 3478 Fragments arranged by size: 3478 1225 1144 361 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6208 PvuII CAG'CTG Cuts at: 2122 2513 2559 3107 2122 Size: 391 46 548 5223 Fragments arranged by size: 5223 548 391 46 SalI G'TCGA_C Cuts at: 1936 3550 1936 Size: 1614 4594 SapI GCTCTTCn'nnn_ Cuts at: 3149 3149 Size: 6208 SbfI CC_TGCA'GG Cuts at: 2055 3560 2055 Size: 1505 4703 ScaI AGT'ACT Cuts at: 4456 6168 4456 Size: 1712 4496 SfcI C'TryA_G Cuts at: 826 1045 2051 2412 2526 3556 4691 5372 5563 Size: 219 1006 361 114 1030 1135 681 191 Cuts at: 5563 826 Size: 1471 Fragments arranged by size: 1471 1135 1030 1006 681 361 219 191 114 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6208 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6208 SmaI CCC'GGG Cuts at: 1872 3535 1872 Size: 1663 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 3042 4312 5213 5475 820 Size: 197 926 1099 1270 901 262 1553 Fragments arranged by size: 1553 1270 1099 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3999 493 Size: 3506 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6208 SphI G_CATG'C Cuts at: 1132 3566 1132 Size: 2434 3774 SspI AAT'ATT Cuts at: 5 52 4132 5 Size: 47 4080 2081 Fragments arranged by size: 4080 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6208 StyI C'CwwG_G Cuts at: 513 1065 2004 2823 3647 513 Size: 552 939 819 824 3074 Fragments arranged by size: 3074 939 824 819 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2377 4235 4252 4405 4590 5035 325 Size: 1255 797 1858 17 153 185 445 1498 Fragments arranged by size: 1858 1498 1255 797 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3350 4454 6166 Size: 274 80 33 51 1443 1373 1104 1712 Cuts at: 6166 96 Size: 138 Fragments arranged by size: 1712 1443 1373 1104 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2245 2277 3267 3299 6168 6200 2245 Size: 32 990 32 2869 32 2253 Fragments arranged by size: 2869 2253 990 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6208 XbaI T'CTAG_A Cuts at: 3544 3544 Size: 6208 XhoI C'TCGA_G Cuts at: 1943 3042 1943 Size: 1099 5109 XmaI C'CCGG_G Cuts at: 1870 3533 1870 Size: 1663 4545 XmnI GAAnn'nnTTC Cuts at: 2689 4337 2689 Size: 1648 4560 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4016 276 Size: 53 83 186 936 2482 2468 Fragments arranged by size: 2482 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI EcoRV FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NsiI NspI PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI PstI PvuI PvuII SalI SapI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BaeI BbeI BplI BsiWI BstXI BstZ17I DraIII EcoICRI EcoNI FalI FseI FspAI KasI NaeI NarI NgoMIV NruI PacI PfoI PmlI PspOMI PspXI PsrI RsrII SacI SacII SanDI SexAI SfiI SfoI SrfI SwaI XcmI