Restriction map for
pFN21AB5613
(Linear) (Six-base) MAPSORT of: KIBB5613.seq Check: 2537 from: 1 to: 1503
KIBB5613.orf
With 182 enzymes: SgfI *
February 28, 2007 11:27 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 139 788 1503
Size: 139 649 715
Fragments arranged by size:
715 649 139
AlwNI CAG_nnn'CTG
Cuts at: 0 415 1213 1503
Size: 415 798 290
Fragments arranged by size:
798 415 290
ApoI r'AATT_y
Cuts at: 0 939 1167 1503
Size: 939 228 336
Fragments arranged by size:
939 336 228
AseI AT'TA_AT
Cuts at: 0 875 1503
Size: 875 628
AvaI C'yCGr_G
Cuts at: 0 1037 1503
Size: 1037 466
BamHI G'GATC_C
Cuts at: 0 566 1503
Size: 566 937
BanI G'GyrC_C
Cuts at: 0 163 1266 1503
Size: 163 1103 237
Fragments arranged by size:
1103 237 163
BbvCI CC'TCA_GC
Cuts at: 0 881 1503
Size: 881 622
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1220 1254 1503
Size: 1220 34 249
Fragments arranged by size:
1220 249 34
BfrBI ATG'CAT
Cuts at: 0 733 1251 1503
Size: 733 518 252
Fragments arranged by size:
733 518 252
BglI GCCn_nnn'nGGC
Cuts at: 0 99 1503
Size: 99 1404
BmrI ACTGGGnnnn_n'
Cuts at: 0 943 1503
Size: 943 560
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 193 341 576 644 999 1166 1397 1503
Size: 193 148 235 68 355 167 231 106
Fragments arranged by size:
355 235 231 193 167 148 106 68
Bpu10I CC'TnA_GC
Cuts at: 0 881 1503
Size: 881 622
BsaI GGTCTCn'nnnn_
Cuts at: 0 1142 1503
Size: 1142 361
BsaBI GATnn'nnATC
Cuts at: 0 1250 1503
Size: 1250 253
BsaHI Gr'CG_yC
Cuts at: 0 668 1503
Size: 668 835
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 382 898 1082 1503
Size: 382 516 184 421
Fragments arranged by size:
516 421 382 184
BseYI C'CCAG_C
Cuts at: 0 1213 1281 1503
Size: 1213 68 222
Fragments arranged by size:
1213 222 68
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 133 1503
Size: 133 1370
BsiHKAI G_wGCw'C
Cuts at: 0 209 1503
Size: 209 1294
BsmBI CGTCTCn'nnnn_
Cuts at: 0 1040 1503
Size: 1040 463
Bsp1286I G_dGCh'C
Cuts at: 0 209 1503
Size: 209 1294
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 53 1503
Size: 53 1450
BsrBI CCG'CTC
Cuts at: 0 927 1503
Size: 927 576
BstAPI GCAn_nnn'nTGC
Cuts at: 0 415 1503
Size: 415 1088
BstEII G'GTnAC_C
Cuts at: 0 40 1045 1503
Size: 40 1005 458
Fragments arranged by size:
1005 458 40
BstYI r'GATC_y
Cuts at: 0 566 1503
Size: 566 937
Bsu36I CC'TnA_GG
Cuts at: 0 197 1503
Size: 197 1306
BtgI C'CryG_G
Cuts at: 0 818 1503
Size: 818 685
BtsI GCAGTG_nn'
Cuts at: 0 911 917 1503
Size: 911 6 586
Fragments arranged by size:
911 586 6
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 887 922 1503
Size: 887 35 581
Fragments arranged by size:
887 581 35
DraI TTT'AAA
Cuts at: 0 663 1503
Size: 663 840
EaeI y'GGCC_r
Cuts at: 0 821 1503
Size: 821 682
EarI CTCTTCn'nnn_
Cuts at: 0 115 675 1144 1312 1503
Size: 115 560 469 168 191
Fragments arranged by size:
560 469 191 168 115
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 139 193 341 576 644 788 999 1166
Size: 139 54 148 235 68 144 211 167
Cuts at: 1166 1397 1503
Size: 231 106
Fragments arranged by size:
235 231 211 167 148 144 139 106
68 54
EcoO109I rG'GnC_Cy
Cuts at: 0 261 1503
Size: 261 1242
EcoRV GAT'ATC
Cuts at: 0 425 1503
Size: 425 1078
HaeII r_GCGC'y
Cuts at: 0 563 1503
Size: 563 940
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 167 199 1503
Size: 167 32 1304
Fragments arranged by size:
1304 167 32
HincII GTy'rAC
Cuts at: 0 60 1503
Size: 60 1443
HpaI GTT'AAC
Cuts at: 0 60 1503
Size: 60 1443
MscI TGG'CCA
Cuts at: 0 823 1503
Size: 823 680
MspA1I CmG'CkG
Cuts at: 0 117 508 554 1102 1503
Size: 117 391 46 548 401
Fragments arranged by size:
548 401 391 117 46
NcoI C'CATG_G
Cuts at: 0 818 1503
Size: 818 685
NdeI CA'TA_TG
Cuts at: 0 735 1503
Size: 735 768
NsiI A_TGCA'T
Cuts at: 0 735 1253 1503
Size: 735 518 250
Fragments arranged by size:
735 518 250
NspI r_CATG'y
Cuts at: 0 234 1304 1503
Size: 234 1070 199
Fragments arranged by size:
1070 234 199
PasI CC'CwG_GG
Cuts at: 0 78 1503
Size: 78 1425
PpuMI rG'GwC_Cy
Cuts at: 0 261 1503
Size: 261 1242
PshAI GACnn'nnGTC
Cuts at: 0 1378 1503
Size: 1378 125
PstI C_TGCA'G
Cuts at: 0 50 411 1503
Size: 50 361 1092
Fragments arranged by size:
1092 361 50
PvuII CAG'CTG
Cuts at: 0 117 508 554 1102 1503
Size: 117 391 46 548 401
Fragments arranged by size:
548 401 391 117 46
SapI GCTCTTCn'nnn_
Cuts at: 0 1144 1503
Size: 1144 359
SbfI CC_TGCA'GG
Cuts at: 0 50 1503
Size: 50 1453
SfcI C'TryA_G
Cuts at: 0 46 407 521 1503
Size: 46 361 114 982
Fragments arranged by size:
982 361 114 46
SmlI C'TyrA_G
Cuts at: 0 1037 1503
Size: 1037 466
StyI C'CwwG_G
Cuts at: 0 818 1503
Size: 818 685
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 372 1503
Size: 372 1131
TatI w'GTAC_w
Cuts at: 0 1345 1503
Size: 1345 158
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 240 272 1262 1294 1503
Size: 240 32 990 32 209
Fragments arranged by size:
990 240 209 32 32
XhoI C'TCGA_G
Cuts at: 0 1037 1503
Size: 1037 466
XmnI GAAnn'nnTTC
Cuts at: 0 684 1503
Size: 684 819
Enzymes that do cut:
AcuI AlwNI ApoI AseI AvaI BamHI BanI BbvCI
BcgI BfrBI BglI BmrI BpmI Bpu10I BsaI BsaBI
BsaHI BseRI BseYI BsgI BsiHKAI BsmBI Bsp1286I BspMI
BsrBI BstAPI BstEII BstYI Bsu36I BtgI BtsI CspCI
DraI EaeI EarI Eco57MI EcoO109I EcoRV HaeII Hin4I
HincII HpaI MscI MspA1I NcoI NdeI NsiI NspI
PasI PpuMI PshAI PstI PvuII SapI SbfI SfcI
SmlI StyI TaqII TatI TstI XhoI XmnI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AfeI AflII AflIII
AgeI AhdI AleI AlfI AloI ApaI ApaLI AscI
AsiSI AvrII BaeI BanII BbeI BbsI BciVI BclI
BglII BlpI Bme1580I BmgBI BmtI BplI BpuEI BsaAI
BsaWI BsaXI BsiEI BsiWI BsmI BspEI BspHI BsrDI
BsrFI BsrGI BssHII BssSI BstBI BstXI BstZ17I BtgZI
ClaI DraIII DrdI EagI EciI EcoICRI EcoNI EcoRI
FalI FseI FspI FspAI HindIII KasI KpnI MfeI
MluI MmeI MslI NaeI NarI NgoMIV NheI NotI
NruI PacI PciI PflMI PfoI PmeI PmlI PpiI
PsiI PspOMI PspXI PsrI PvuI RsrII SacI SacII
SalI SanDI ScaI SexAI SfiI SfoI SgfI SgrAI
SmaI SnaBI SpeI SphI SrfI SspI StuI SwaI
Tth111I XbaI XcmI XmaI ZraI