Restriction map for
pFN21AB5591
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# NO CUT ENZYMES ( 81 enzymes) #
##################################
AatII Acc65I AclI AgeI AhdI
Alw44I Aor51HI ApaI ApaLI AscI
AseI AsnI Asp718I AspEI AspI
AvrII BbrPI BbsI BbvCI BcgI
BclI BlnI BlpI Bpu1102I BpuAI
BsaAI BsaI BsiWI BspDI BspHI
BssHII BssSI CelII ClaI CpoI
DraI DrdI Eam1105I Eco47III EcoNI
EcoRV EcoT22I FbaI FseI HincII
HindII HindIII HpaI KpnI MluI
NdeI NheI NruI NsiI PacI
PflFI PinAI PmaCI PmeI PmlI
PshAI PshBI Psp1406I PvuI PvuII
RcaI RsrII SalI SbfI SfiI
SmaI SnaBI SpeI SphI Sse8387I
SspI SwaI Tth111I XbaI XmaCI
XmaI
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# ONE CUT ENZYMES ( 50 enzymes) #
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AccI 1717 GTATAC GT^[AC][GT]AC
AflII 2731 CTTAAG C^TTAAG
AflIII 660 ACATGT A^C[GA][CT]GT
Asp700I 1606 GAATACATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AviII 1562 TGCGCA TGC^GCA
BanI 2527 GGCGCC G^G[CT][GA]CC
BbeI 2527 GGCGCC GGCGC^C
BciVI 504 GTATCC GTATCC(6/5)
BfaI 814 CTAG C^TAG
BfrI 2731 CTTAAG C^TTAAG
BsaBI 552 GATGGAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsmBI 1804 GAGACG CGTCTC(1/5)
BsmI 653 GCATTC GAATGC(1/-1)
Bsp1407I 2561 TGTACA T^GTACA
BspLU11I 660 ACATGT A^CATGT
BsrBI 1410 CCGCTC CCGCTC(-3/-3)
BsrGI 2561 TGTACA T^GTACA
Bst1107I 1717 GTATAC GTA^TAC
BstAPI 2190 GCAGGTGGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstBI 1645 TTCGAA TT^CGAA
BstEII 130 GGTTACC G^GT[ACGT]ACC
BstPI 130 GGTTACC G^GT[ACGT]ACC
BstXI 1304 CCAACATGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstZ17I 1717 GTATAC GTA^TAC
EagI 2172 CGGCCG C^GGCCG
EclXI 2172 CGGCCG C^GGCCG
Eco52I 2172 CGGCCG C^GGCCG
EcoO65I 130 GGTTACC G^GT[ACGT]ACC
EcoRI 96 GAATTC G^AATTC
FspI 1562 TGCGCA TGC^GCA
KasI 2527 GGCGCC G^GCGCC
MaeI 814 CTAG C^TAG
MamI 552 GATGGAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MfeI 1932 CAATTG C^AATTG
MunI 1932 CAATTG C^AATTG
NarI 2527 GGCGCC GG^CGCC
NcoI 933 CCATGG C^CATGG
NotI 2171 GCGGCCGC GC^GGCCGC
NspV 1645 TTCGAA TT^CGAA
PaeR7I 1951 CTCGAG C^TCGAG
PflMI 658 CCACATGTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
ScaI 683 AGTACT AGT^ACT
SexAI 794 ACCTGGT A^CC[AT]GGT
SfoI 2527 GGCGCC GGC^GCC
SfuI 1645 TTCGAA TT^CGAA
SgrAI 1736 CGCCGGCG C[GA]^CCGG[CT]G
SspBI 2561 TGTACA T^GTACA
Van91I 658 CCACATGTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XhoI 1951 CTCGAG C^TCGAG
XmnI 1606 GAATACATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 34 enzymes) #
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AccIII 1994 TCCGGA T^CCGGA
2341 TCCGGA
AcsI 96 GAATTC [GA]^AATT[CT]
2052 GAATTT
AcyI 2527 GGCGCC G[GA]^CG[CT]C
2611 GGCGTC
AocI 1435 CCTCAGG CC^T[ACGT]AGG
2516 CCTCAGG
ApoI 96 GAATTC [GA]^AATT[CT]
2052 GAATTT
BalI 539 TGGCCA TGG^CCA
764 TGGCCA
BamHI 728 GGATCC G^GATCC
2571 GGATCC
BglI 1409 GCCGCTCGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
1586 GCCTAAACGGC
BsaHI 2527 GGCGCC G[GA]^CG[CT]C
2611 GGCGTC
BseAI 1994 TCCGGA T^CCGGA
2341 TCCGGA
BspEI 1994 TCCGGA T^CCGGA
2341 TCCGGA
BsrDI 1332 CATTGC GCAATG(2/0)
830 GCAATG
Bsu36I 1435 CCTCAGG CC^T[ACGT]AGG
2516 CCTCAGG
DraIII 52 CACCAAGTG CAC[ACGT][ACGT][ACGT]^GTG
1869 CACAGAGTG
Eco81I 1435 CCTCAGG CC^T[ACGT]AGG
2516 CCTCAGG
EcoT14I 767 CCAAGG C^C[AT][AT]GG
933 CCATGG
HaeII 2184 GGCGCT [GA]GCGC^[CT]
2527 GGCGCC
HgaI 1806 GACGC GACGC(5/10)
2612 GCGTC
Hin1I 2527 GGCGCC G[GA]^CG[CT]C
2611 GGCGTC
KspI 1518 CCGCGG CCGC^GG
2175 CCGCGG
MaeII 2035 ACGT A^CGT
2337 ACGT
MluNI 539 TGGCCA TGG^CCA
764 TGGCCA
MroI 1994 TCCGGA T^CCGGA
2341 TCCGGA
MscI 539 TGGCCA TGG^CCA
764 TGGCCA
MseI 1006 TTAA T^TAA
2732 TTAA
MspA1I 1518 CCGCGG C[AC]G^C[GT]G
2175 CCGCGG
NaeI 926 GCCGGC GCC^GGC
1737 GCCGGC
NgoMIV 926 GCCGGC G^CCGGC
1737 GCCGGC
SacII 1518 CCGCGG CCGC^GG
2175 CCGCGG
SapI 1824 GAAGAGC GCTCTTC(1/4)
1269 GCTCTTC
SmlI 1951 CTCGAG C^T[CT][GA]AG
2731 CTTAAG
StuI 1326 AGGCCT AGG^CCT
2637 AGGCCT
StyI 767 CCAAGG C^C[AT][AT]GG
933 CCATGG
Tru9I 1006 TTAA T^TAA
2732 TTAA
#########################################
# 3 OR MORE CUT ENZYMES ( 93 enzymes) #
#########################################
AccII AciI AfaI AluI AlwI
AlwNI AspHI AvaI AvaII BanII
BbvI BcnI BglII BmyI BpmI
BsaJI BsaWI BseRI BsgI BsiEI
BsiHKAI BsiYI BslI BsmAI BsmFI
BsoBI Bsp1286I BspMI BsrFI BsrI
BssKI BstF5I BstNI BstUI BstYI
Cac8I CfoI Cfr10I Cfr13I DdeI
DpnII DraII DsaI EaeI EarI
EcoO109I EcoRII FauI Fnu4HI FokI
HaeIII HapII HhaI HinP1I HinfI
HpaII HphI ItaI Ksp632I MaeIII
MboI MboII MflI MnlI MslI
MspI MvaI MvnI MwoI NciI
NdeII NlaIII NlaIV NspI PleI
PpuMI PstI RsaI SacI Sau3AI
Sau96I ScrFI SfaNI SfcI TaqI
TfiI TseI Tsp45I Tsp509I TspRI
TthHB8I XcmI XhoII