(Circular) (Six-base) MAPSORT of: pFN21AB5591.seq Check: 4670 from: 1 to: 7483 pFN21AB5591 7483 bp With 182 enzymes: SgfI * February 27, 2009 14:34 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2599 4137 4186 2599 Size: 1538 49 5896 Fragments arranged by size: 5896 1538 49 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 5293 278 Size: 53 83 186 936 3757 2468 Fragments arranged by size: 3757 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3723 4826 1937 Size: 1786 1103 4594 Fragments arranged by size: 4594 1786 1103 Acc65I G'GTAC_C Cuts at: 4804 4804 Size: 7483 AclI AA'CG_TT Cuts at: 1563 5610 5983 1563 Size: 4047 373 3063 Fragments arranged by size: 4047 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2463 3452 5546 6930 7198 1632 Size: 831 989 2094 1384 268 1917 Fragments arranged by size: 2094 1917 1384 989 831 268 AflII C'TTAA_G Cuts at: 820 1017 4736 820 Size: 197 3719 3567 Fragments arranged by size: 3719 3567 197 AflIII A'CryG_T Cuts at: 1203 2665 6382 7359 1203 Size: 1462 3717 977 1327 Fragments arranged by size: 3717 1462 1327 977 AgeI A'CCGG_T Cuts at: 6314 6314 Size: 7483 AhdI GACnn_n'nnGTC Cuts at: 6212 6212 Size: 7483 AleI CACnn'nnGTG Cuts at: 1166 3545 1166 Size: 2379 5104 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 7483 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 4327 4359 4327 Size: 32 7451 AlwNI CAG_nnn'CTG Cuts at: 2441 2578 4529 5226 6798 2441 Size: 137 1951 697 1572 3126 Fragments arranged by size: 3126 1951 1572 697 137 ApaLI G'TGCA_C Cuts at: 5540 6696 5540 Size: 1156 6327 ApoI r'AATT_y Cuts at: 1493 2101 4057 4792 5018 5170 7279 1493 Size: 608 1956 735 226 152 2109 1697 Fragments arranged by size: 2109 1956 1697 735 608 226 152 AseI AT'TA_AT Cuts at: 160 1032 6037 160 Size: 872 5005 1606 Fragments arranged by size: 5005 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 7483 AvaI C'yCGr_G Cuts at: 1870 1943 2350 3418 3956 4672 4808 1870 Size: 73 407 1068 538 716 136 4545 Fragments arranged by size: 4545 1068 716 538 407 136 73 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 3238 3271 3238 Size: 33 7450 BamHI G'GATC_C Cuts at: 2733 4576 4813 5180 2733 Size: 1843 237 367 5036 Fragments arranged by size: 5036 1843 367 237 BanI G'GyrC_C Cuts at: 618 943 1160 1787 4532 4804 6259 618 Size: 325 217 627 2745 272 1455 1842 Fragments arranged by size: 2745 1842 1455 627 325 272 217 BanII G_rGCy'C Cuts at: 1391 2357 3276 3389 3523 3966 4610 4685 4725 Size: 966 919 113 134 443 644 75 40 Cuts at: 4725 4802 1391 Size: 77 4072 Fragments arranged by size: 4072 966 919 644 443 134 113 77 75 40 BbeI G_GCGC'C Cuts at: 4536 4536 Size: 7483 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 6512 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 5674 5708 1903 Size: 34 3737 34 3678 Fragments arranged by size: 3737 3678 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2520 5375 6585 2520 Size: 2855 1210 3418 Fragments arranged by size: 3418 2855 1210 BclI T'GATC_A Cuts at: 1548 1548 Size: 7483 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 3420 3597 6094 Size: 107 122 71 631 605 1748 177 2497 Cuts at: 6094 136 Size: 1525 Fragments arranged by size: 2497 1748 1525 631 605 177 122 107 71 BglII A'GATC_T Cuts at: 2424 3351 3475 7478 2424 Size: 927 124 4003 2429 Fragments arranged by size: 4003 2429 927 124 BlpI GC'TnA_GC Cuts at: 4900 4900 Size: 7483 Bme1580I G_kGCm'C Cuts at: 1790 5544 6700 1790 Size: 3754 1156 2573 Fragments arranged by size: 3754 2573 1156 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 7483 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3384 3616 4622 Size: 416 53 83 399 24 1967 232 1006 Cuts at: 4622 5214 6172 442 Size: 592 958 1753 Fragments arranged by size: 1967 1753 1006 958 592 416 399 232 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 7483 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2612 2975 3290 3403 3785 4006 4415 4519 Size: 1373 363 315 113 382 221 409 104 Cuts at: 4519 4724 6143 1239 Size: 205 1419 2579 Fragments arranged by size: 2579 1419 1373 409 382 363 315 221 205 113 104 Bpu10I CC'TnA_GC Cuts at: 4679 4679 Size: 7483 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 5608 6473 6771 5608 Size: 865 298 6320 Fragments arranged by size: 6320 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 6146 882 Size: 617 4647 2219 Fragments arranged by size: 4647 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 5274 493 Size: 711 4070 2702 Fragments arranged by size: 4070 2702 711 BsaBI GATnn'nnATC Cuts at: 2561 7477 2561 Size: 4916 2567 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 4533 4617 5290 5672 Size: 53 83 186 936 3000 84 673 382 Cuts at: 5672 6307 275 Size: 635 1451 Fragments arranged by size: 3000 1451 936 673 635 382 186 84 83 53 BsaWI w'CCGG_w Cuts at: 3999 4035 4346 5916 6314 6588 6735 7146 3999 Size: 36 311 1570 398 274 147 411 4336 Fragments arranged by size: 4336 1570 411 398 311 274 147 36 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2138 2168 3283 3313 3376 Size: 30 165 30 735 30 1115 30 63 Cuts at: 3376 3406 3722 3752 4145 4175 4211 4241 1178 Size: 30 316 30 393 30 36 30 4420 Fragments arranged by size: 4420 1115 735 393 316 165 63 36 30 30 30 30 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2338 3473 3543 3881 3974 1186 Size: 1152 1135 70 338 93 4695 Fragments arranged by size: 4695 1152 1135 338 93 70 BseYI C'CCAG_C Cuts at: 3316 3447 3633 3772 3814 4289 6686 7182 3316 Size: 131 186 139 42 475 2397 496 3617 Fragments arranged by size: 3617 2397 496 475 186 139 131 42 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2512 3414 4642 4659 4678 1163 Size: 573 776 902 1228 17 19 3968 Fragments arranged by size: 3968 1228 902 776 573 19 17 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 4036 4180 4545 4933 5694 6722 Size: 275 186 2035 144 365 388 761 1028 Cuts at: 6722 1540 Size: 2301 Fragments arranged by size: 2301 2035 1028 761 388 365 275 186 144 BsiHKAI G_wGCw'C Cuts at: 3276 4610 4685 4725 5544 5629 6700 3276 Size: 1334 75 40 819 85 1071 4059 Fragments arranged by size: 4059 1334 1071 819 85 75 40 BsmI GAATG_Cn' Cuts at: 2658 4994 5087 2658 Size: 2336 93 5054 Fragments arranged by size: 5054 2336 93 BsmBI CGTCTCn'nnnn_ Cuts at: 3803 3803 Size: 7483 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2357 3276 3389 3523 3966 4610 4685 Size: 399 567 919 113 134 443 644 75 Cuts at: 4685 4725 4802 5544 5629 6700 1391 Size: 40 77 742 85 1071 2174 Fragments arranged by size: 2174 1071 919 742 644 567 443 399 134 113 85 77 75 40 BspEI T'CCGG_A Cuts at: 3999 4346 7146 3999 Size: 347 2800 4336 Fragments arranged by size: 4336 2800 347 BspHI T'CATG_A Cuts at: 5372 7102 5372 Size: 1730 5753 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2599 3009 4137 4186 4838 844 Size: 1755 410 1128 49 652 3489 Fragments arranged by size: 3489 1755 1128 652 410 49 BsrBI CCG'CTC Cuts at: 1394 1823 3417 5370 1394 Size: 429 1594 1953 3507 Fragments arranged by size: 3507 1953 1594 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2842 3336 5978 6152 65 Size: 1183 1594 494 2642 174 1396 Fragments arranged by size: 2642 1594 1396 1183 494 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2931 3129 3742 6127 6314 1700 Size: 110 1121 198 613 2385 187 2869 Fragments arranged by size: 2869 2385 1121 613 198 187 110 BsrGI T'GTAC_A Cuts at: 96 4566 96 Size: 4470 3013 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 7483 BssSI C'ACGA_G Cuts at: 5543 6555 5543 Size: 1012 6471 BstAPI GCAn_nnn'nTGC Cuts at: 794 4201 794 Size: 3407 4076 BstBI TT'CG_AA Cuts at: 3651 6287 3651 Size: 2636 4847 BstEII G'GTnAC_C Cuts at: 1184 2135 1184 Size: 951 6532 BstXI CCAn_nnnn'nTGG Cuts at: 3316 3316 Size: 7483 BstYI r'GATC_y Cuts at: 1972 2424 2733 3351 3475 4576 4813 5180 5565 Size: 452 309 618 124 1101 237 367 385 Cuts at: 5565 5582 7034 7120 7132 7478 1972 Size: 17 1452 86 12 346 1977 Fragments arranged by size: 1977 1452 1101 618 452 385 367 346 309 237 124 86 17 12 BstZ17I GTA'TAC Cuts at: 3724 3724 Size: 7483 Bsu36I CC'TnA_GG Cuts at: 3441 4522 3441 Size: 1081 6402 BtgI C'CryG_G Cuts at: 513 1065 2004 2938 3523 3801 4170 4180 513 Size: 552 939 934 585 278 369 10 3816 Fragments arranged by size: 3816 939 934 585 552 369 278 10 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2311 3013 4582 487 Size: 683 56 1085 702 1569 3388 Fragments arranged by size: 3388 1569 1085 702 683 56 BtsI GCAGTG_nn' Cuts at: 3206 3540 4434 4999 5793 5813 3206 Size: 334 894 565 794 20 4876 Fragments arranged by size: 4876 894 794 565 334 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 7483 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 2299 2334 2502 2537 2674 2709 3162 Size: 35 1735 35 168 35 137 35 453 Cuts at: 3162 3197 529 Size: 35 4815 Fragments arranged by size: 4815 1735 453 168 137 35 35 35 35 35 DraI TTT'AAA Cuts at: 4787 4787 Size: 7483 DraIII CAC_nnn'GTG Cuts at: 2062 3879 2062 Size: 1817 5666 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 6490 809 Size: 507 5174 1802 Fragments arranged by size: 5174 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2544 2769 2860 Size: 54 1425 50 275 18 714 225 91 Cuts at: 2860 3024 4177 4930 5819 7448 8 Size: 164 1153 753 889 1629 43 Fragments arranged by size: 1629 1425 1153 889 753 714 275 225 164 91 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 4177 4930 1537 Size: 275 2365 753 4090 Fragments arranged by size: 4090 2365 753 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3281 3367 3824 3831 3858 5413 1353 Size: 1928 86 457 7 27 1555 3423 Fragments arranged by size: 3423 1928 1555 457 86 27 7 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 3221 3346 4291 6070 6444 6590 Size: 1064 138 1632 125 945 1779 374 146 Cuts at: 6590 387 Size: 1280 Fragments arranged by size: 1779 1632 1280 1064 945 374 146 138 125 EcoICRI GAG'CTC Cuts at: 3274 4608 4683 4723 3274 Size: 1334 75 40 6034 Fragments arranged by size: 6034 1334 75 40 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2463 2612 2975 3290 3403 3452 3785 Size: 393 831 149 363 315 113 49 333 Cuts at: 3785 4006 4415 4519 4724 5546 6143 6930 7198 Size: 221 409 104 205 822 597 787 268 Cuts at: 7198 1239 Size: 1524 Fragments arranged by size: 1524 831 822 787 597 409 393 363 333 315 268 221 205 149 113 104 49 EcoO109I rG'GnC_Cy Cuts at: 3622 3630 3818 3622 Size: 8 188 7287 Fragments arranged by size: 7287 188 8 EcoRI G'AATT_C Cuts at: 2101 4792 5170 2101 Size: 2691 378 4414 Fragments arranged by size: 4414 2691 378 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2278 2797 2278 Size: 519 6964 FspI TGC'GCA Cuts at: 3569 5989 3569 Size: 2420 5063 HaeII r_GCGC'y Cuts at: 1678 4193 4536 6630 1678 Size: 2515 343 2094 2531 Fragments arranged by size: 2531 2515 2094 343 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2138 2170 2276 2308 2768 Size: 32 135 32 566 32 106 32 460 Cuts at: 2768 2800 3522 3554 3722 3754 6124 6156 6198 Size: 32 722 32 168 32 2370 32 42 Cuts at: 6198 6230 1373 Size: 32 2626 Fragments arranged by size: 2626 2370 722 566 460 168 135 106 42 32 32 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 4827 5073 669 Size: 988 281 2889 246 3079 Fragments arranged by size: 3079 2889 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 7483 HpaI GTT'AAC Cuts at: 5073 5073 Size: 7483 KasI G'GCGC_C Cuts at: 4532 4532 Size: 7483 KpnI G_GTAC'C Cuts at: 4808 4808 Size: 7483 MfeI C'AATT_G Cuts at: 3937 5082 3937 Size: 1145 6338 MluI A'CGCG_T Cuts at: 7359 7359 Size: 7483 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2406 3000 3333 3953 6597 6781 1305 Size: 1101 594 333 620 2644 184 2007 Fragments arranged by size: 2644 2007 1101 620 594 333 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2546 2771 10 Size: 54 1425 343 714 225 4722 Fragments arranged by size: 4722 1425 714 343 225 54 MslI CAynn'nnrTG Cuts at: 518 1166 2009 2759 2765 2943 3545 4601 5441 Size: 648 843 750 6 178 602 1056 840 Cuts at: 5441 5800 5959 518 Size: 359 159 2042 Fragments arranged by size: 2042 1056 843 840 750 648 602 359 178 159 6 MspA1I CmG'CkG Cuts at: 1606 3525 4182 4899 5576 6724 6969 1606 Size: 1919 657 717 677 1148 245 2120 Fragments arranged by size: 2120 1919 1148 717 677 657 245 NaeI GCC'GGC Cuts at: 2933 3744 2933 Size: 811 6672 NarI GG'CG_CC Cuts at: 4533 4533 Size: 7483 NcoI C'CATG_G Cuts at: 513 1065 2004 2938 513 Size: 552 939 934 5058 Fragments arranged by size: 5058 939 934 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 7483 NgoMIV G'CCGG_C Cuts at: 2931 3742 2931 Size: 811 6672 NheI G'CTAG_C Cuts at: 1052 1052 Size: 7483 NotI GC'GGCC_GC Cuts at: 4177 4930 4177 Size: 753 6730 NspI r_CATG'y Cuts at: 1132 1229 2669 3316 3664 4841 6386 1132 Size: 97 1440 647 348 1177 1545 2229 Fragments arranged by size: 2229 1545 1440 1177 647 348 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 7483 PciI A'CATG_T Cuts at: 2665 6382 2665 Size: 3717 3766 PflMI CCAn_nnn'nTGG Cuts at: 1108 2669 1108 Size: 1561 5922 PmeI GTTT'AAAC Cuts at: 4787 4787 Size: 7483 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 5552 5584 7065 7097 5552 Size: 32 1481 32 5938 Fragments arranged by size: 5938 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 3622 3630 3818 3622 Size: 8 188 7287 Fragments arranged by size: 7287 188 8 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 7483 PsiI TTA'TAA Cuts at: 5053 5053 Size: 7483 PspXI vC'TCGA_Gb Cuts at: 3956 3956 Size: 7483 PstI C_TGCA'G Cuts at: 830 2574 3896 4074 4197 4835 830 Size: 1744 1322 178 123 638 3478 Fragments arranged by size: 3478 1744 1322 638 178 123 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 7483 SacI G_AGCT'C Cuts at: 3276 4610 4685 4725 3276 Size: 1334 75 40 6034 Fragments arranged by size: 6034 1334 75 40 SacII CC_GC'GG Cuts at: 3526 4183 3526 Size: 657 6826 SalI G'TCGA_C Cuts at: 1936 4825 1936 Size: 2889 4594 SanDI GG'GwC_CC Cuts at: 3818 3818 Size: 7483 SapI GCTCTTCn'nnn_ Cuts at: 3281 3824 3281 Size: 543 6940 SbfI CC_TGCA'GG Cuts at: 4835 4835 Size: 7483 ScaI AGT'ACT Cuts at: 2690 5731 7443 2690 Size: 3041 1712 2730 Fragments arranged by size: 3041 2730 1712 SexAI A'CCwGG_T Cuts at: 2799 2799 Size: 7483 SfcI C'TryA_G Cuts at: 826 1045 2570 3586 3892 4070 4193 4831 5966 Size: 219 1525 1016 306 178 123 638 1135 Cuts at: 5966 6647 6838 826 Size: 681 191 1471 Fragments arranged by size: 1525 1471 1135 1016 681 638 306 219 191 178 123 SfoI GGC'GCC Cuts at: 4534 4534 Size: 7483 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 7483 SgrAI Cr'CCGG_yG Cuts at: 1700 3742 1700 Size: 2042 5441 SmaI CCC'GGG Cuts at: 1872 4810 1872 Size: 2938 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 3956 4736 5587 6488 6750 820 Size: 197 926 2013 780 851 901 262 1553 Fragments arranged by size: 2013 1553 926 901 851 780 262 197 SnaBI TAC'GTA Cuts at: 493 5274 493 Size: 4781 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 7483 SphI G_CATG'C Cuts at: 1132 4841 1132 Size: 3709 3774 SspI AAT'ATT Cuts at: 5 52 5407 5 Size: 47 5355 2081 Fragments arranged by size: 5355 2081 47 StuI AGG'CCT Cuts at: 1517 3333 4644 1517 Size: 1816 1311 4356 Fragments arranged by size: 4356 1816 1311 StyI C'CwwG_G Cuts at: 513 1065 2004 2772 2938 4922 513 Size: 552 939 768 166 1984 3074 Fragments arranged by size: 3074 1984 939 768 552 166 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3383 3502 4559 5510 5527 5680 5865 Size: 1255 1803 119 1057 951 17 153 185 Cuts at: 5865 6310 325 Size: 445 1498 Fragments arranged by size: 1803 1498 1255 1057 951 445 185 153 119 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2688 4566 5729 Size: 274 80 33 51 1443 711 1878 1163 Cuts at: 5729 7441 96 Size: 1712 138 Fragments arranged by size: 1878 1712 1443 1163 711 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3283 3315 3376 3408 3810 3842 4561 4593 4602 Size: 32 61 32 402 32 719 32 9 Cuts at: 4602 4634 7443 7475 3283 Size: 32 2809 32 3291 Fragments arranged by size: 3291 2809 719 402 61 32 32 32 32 32 32 9 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 7483 XbaI T'CTAG_A Cuts at: 4819 4819 Size: 7483 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2554 4297 4609 2554 Size: 1743 312 5428 Fragments arranged by size: 5428 1743 312 XhoI C'TCGA_G Cuts at: 1943 3956 1943 Size: 2013 5470 XmaI C'CCGG_G Cuts at: 1870 4808 1870 Size: 2938 4545 XmnI GAAnn'nnTTC Cuts at: 3615 5612 3615 Size: 1997 5486 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 5291 276 Size: 53 83 186 936 3757 2468 Fragments arranged by size: 3757 2468 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaLI ApoI AseI AsiSI AvaI BaeI BamHI BanI BanII BbeI BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BstZ17I Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI PspXI PstI PvuI SacI SacII SalI SanDI SapI SbfI ScaI SexAI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI ApaI AscI AvrII BbvCI BfrBI BplI BsiWI EcoNI EcoRV FseI FspAI NruI NsiI PacI PfoI PmlI PspOMI PsrI PvuII RsrII SfiI SrfI SwaI