Restriction map for
pFN21AB5591
(Linear) (Six-base) MAPSORT of: KIBB5591.seq Check: 3031 from: 1 to: 2778
KIBB5591.orf
With 182 enzymes: SgfI *
February 28, 2007 11:27 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 594 2132 2181 2778
Size: 594 1538 49 597
Fragments arranged by size:
1538 597 594 49
AccI GT'mk_AC
Cuts at: 0 1718 2778
Size: 1718 1060
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 458 1447 2778
Size: 458 989 1331
Fragments arranged by size:
1331 989 458
AflII C'TTAA_G
Cuts at: 0 2731 2778
Size: 2731 47
AflIII A'CryG_T
Cuts at: 0 660 2778
Size: 660 2118
AleI CACnn'nnGTG
Cuts at: 0 1540 2778
Size: 1540 1238
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 2322 2354 2778
Size: 2322 32 424
Fragments arranged by size:
2322 424 32
AlwNI CAG_nnn'CTG
Cuts at: 0 436 573 2524 2778
Size: 436 137 1951 254
Fragments arranged by size:
1951 436 254 137
ApoI r'AATT_y
Cuts at: 0 96 2052 2778
Size: 96 1956 726
Fragments arranged by size:
1956 726 96
AvaI C'yCGr_G
Cuts at: 0 345 1413 1951 2667 2778
Size: 345 1068 538 716 111
Fragments arranged by size:
1068 716 538 345 111
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 0 1233 1266 2778
Size: 1233 33 1512
Fragments arranged by size:
1512 1233 33
BamHI G'GATC_C
Cuts at: 0 728 2571 2778
Size: 728 1843 207
Fragments arranged by size:
1843 728 207
BanI G'GyrC_C
Cuts at: 0 2527 2778
Size: 2527 251
BanII G_rGCy'C
Cuts at: 0 352 1271 1384 1518 1961 2605 2680 2720
Size: 352 919 113 134 443 644 75 40
Cuts at: 2720 2778
Size: 58
Fragments arranged by size:
919 644 443 352 134 113 75 58
40
BbeI G_GCGC'C
Cuts at: 0 2531 2778
Size: 2531 247
BciVI GTATCCnnnnn_n'
Cuts at: 0 515 2778
Size: 515 2263
BglI GCCn_nnn'nGGC
Cuts at: 0 1415 1592 2778
Size: 1415 177 1186
Fragments arranged by size:
1415 1186 177
BglII A'GATC_T
Cuts at: 0 419 1346 1470 2778
Size: 419 927 124 1308
Fragments arranged by size:
1308 927 419 124
BmrI ACTGGGnnnn_n'
Cuts at: 0 1379 1611 2617 2778
Size: 1379 232 1006 161
Fragments arranged by size:
1379 1006 232 161
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 607 970 1285 1398 1780 2001 2410 2514
Size: 607 363 315 113 382 221 409 104
Cuts at: 2514 2719 2778
Size: 205 59
Fragments arranged by size:
607 409 382 363 315 221 205 113
104 59
Bpu10I CC'TnA_GC
Cuts at: 0 2674 2778
Size: 2674 104
BsaBI GATnn'nnATC
Cuts at: 0 556 2778
Size: 556 2222
BsaHI Gr'CG_yC
Cuts at: 0 2528 2612 2778
Size: 2528 84 166
Fragments arranged by size:
2528 166 84
BsaWI w'CCGG_w
Cuts at: 0 1994 2030 2341 2778
Size: 1994 36 311 437
Fragments arranged by size:
1994 437 311 36
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 133 163 1278 1308 1371 1401 1717 1747
Size: 133 30 1115 30 63 30 316 30
Cuts at: 1747 2140 2170 2206 2236 2778
Size: 393 30 36 30 542
Fragments arranged by size:
1115 542 393 316 133 63 36 30
30 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 333 1468 1538 1876 1969 2778
Size: 333 1135 70 338 93 809
Fragments arranged by size:
1135 809 338 333 93 70
BseYI C'CCAG_C
Cuts at: 0 1311 1442 1628 1767 1809 2284 2778
Size: 1311 131 186 139 42 475 494
Fragments arranged by size:
1311 494 475 186 139 131 42
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 507 1409 2637 2654 2673 2778
Size: 507 902 1228 17 19 105
Fragments arranged by size:
1228 902 507 105 19 17
BsiEI CG_ry'CG
Cuts at: 0 2031 2175 2540 2778
Size: 2031 144 365 238
Fragments arranged by size:
2031 365 238 144
BsiHKAI G_wGCw'C
Cuts at: 0 1271 2605 2680 2720 2778
Size: 1271 1334 75 40 58
Fragments arranged by size:
1334 1271 75 58 40
BsmI GAATG_Cn'
Cuts at: 0 653 2778
Size: 653 2125
BsmBI CGTCTCn'nnnn_
Cuts at: 0 1798 2778
Size: 1798 980
Bsp1286I G_dGCh'C
Cuts at: 0 352 1271 1384 1518 1961 2605 2680 2720
Size: 352 919 113 134 443 644 75 40
Cuts at: 2720 2778
Size: 58
Fragments arranged by size:
919 644 443 352 134 113 75 58
40
BspEI T'CCGG_A
Cuts at: 0 1994 2341 2778
Size: 1994 347 437
Fragments arranged by size:
1994 437 347
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 594 1004 2132 2181 2778
Size: 594 410 1128 49 597
Fragments arranged by size:
1128 597 594 410 49
BsrBI CCG'CTC
Cuts at: 0 1412 2778
Size: 1412 1366
BsrDI GCAATG_nn'
Cuts at: 0 837 1331 2778
Size: 837 494 1447
Fragments arranged by size:
1447 837 494
BsrFI r'CCGG_y
Cuts at: 0 926 1124 1737 2778
Size: 926 198 613 1041
Fragments arranged by size:
1041 926 613 198
BsrGI T'GTAC_A
Cuts at: 0 2561 2778
Size: 2561 217
BstAPI GCAn_nnn'nTGC
Cuts at: 0 2196 2778
Size: 2196 582
BstBI TT'CG_AA
Cuts at: 0 1646 2778
Size: 1646 1132
BstEII G'GTnAC_C
Cuts at: 0 130 2778
Size: 130 2648
BstXI CCAn_nnnn'nTGG
Cuts at: 0 1311 2778
Size: 1311 1467
BstYI r'GATC_y
Cuts at: 0 419 728 1346 1470 2571 2778
Size: 419 309 618 124 1101 207
Fragments arranged by size:
1101 618 419 309 207 124
BstZ17I GTA'TAC
Cuts at: 0 1719 2778
Size: 1719 1059
Bsu36I CC'TnA_GG
Cuts at: 0 1436 2517 2778
Size: 1436 1081 261
Fragments arranged by size:
1436 1081 261
BtgI C'CryG_G
Cuts at: 0 933 1518 1796 2165 2175 2778
Size: 933 585 278 369 10 603
Fragments arranged by size:
933 603 585 369 278 10
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 306 1008 2577 2778
Size: 306 702 1569 201
Fragments arranged by size:
1569 702 306 201
BtsI GCAGTG_nn'
Cuts at: 0 1201 1535 2429 2778
Size: 1201 334 894 349
Fragments arranged by size:
1201 894 349 334
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 294 329 497 532 669 704 1157 1192
Size: 294 35 168 35 137 35 453 35
Cuts at: 1192 2778
Size: 1586
Fragments arranged by size:
1586 453 294 168 137 35 35 35
35
DraIII CAC_nnn'GTG
Cuts at: 0 57 1874 2778
Size: 57 1817 904
Fragments arranged by size:
1817 904 57
EaeI y'GGCC_r
Cuts at: 0 539 764 855 1019 2172 2778
Size: 539 225 91 164 1153 606
Fragments arranged by size:
1153 606 539 225 164 91
EagI C'GGCC_G
Cuts at: 0 2172 2778
Size: 2172 606
EarI CTCTTCn'nnn_
Cuts at: 0 1276 1362 1819 1826 1853 2778
Size: 1276 86 457 7 27 925
Fragments arranged by size:
1276 925 457 86 27 7
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 1216 1341 2286 2778
Size: 1216 125 945 492
Fragments arranged by size:
1216 945 492 125
EcoICRI GAG'CTC
Cuts at: 0 1269 2603 2678 2718 2778
Size: 1269 1334 75 40 60
Fragments arranged by size:
1334 1269 75 60 40
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 458 607 970 1285 1398 1447 1780 2001
Size: 458 149 363 315 113 49 333 221
Cuts at: 2001 2410 2514 2719 2778
Size: 409 104 205 59
Fragments arranged by size:
458 409 363 333 315 221 205 149
113 104 59 49
EcoO109I rG'GnC_Cy
Cuts at: 0 1617 1625 1813 2778
Size: 1617 8 188 965
Fragments arranged by size:
1617 965 188 8
EcoRI G'AATT_C
Cuts at: 0 96 2778
Size: 96 2682
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 273 792 2778
Size: 273 519 1986
Fragments arranged by size:
1986 519 273
FspI TGC'GCA
Cuts at: 0 1564 2778
Size: 1564 1214
HaeII r_GCGC'y
Cuts at: 0 2188 2531 2778
Size: 2188 343 247
Fragments arranged by size:
2188 343 247
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 133 165 271 303 763 795 1517 1549
Size: 133 32 106 32 460 32 722 32
Cuts at: 1549 1717 1749 2778
Size: 168 32 1029
Fragments arranged by size:
1029 722 460 168 133 106 32 32
32 32 32
KasI G'GCGC_C
Cuts at: 0 2527 2778
Size: 2527 251
MfeI C'AATT_G
Cuts at: 0 1932 2778
Size: 1932 846
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 401 995 1328 1948 2778
Size: 401 594 333 620 830
Fragments arranged by size:
830 620 594 401 333
MscI TGG'CCA
Cuts at: 0 541 766 2778
Size: 541 225 2012
Fragments arranged by size:
2012 541 225
MslI CAynn'nnrTG
Cuts at: 0 754 760 938 1540 2596 2778
Size: 754 6 178 602 1056 182
Fragments arranged by size:
1056 754 602 182 178 6
MspA1I CmG'CkG
Cuts at: 0 1520 2177 2778
Size: 1520 657 601
Fragments arranged by size:
1520 657 601
NaeI GCC'GGC
Cuts at: 0 928 1739 2778
Size: 928 811 1039
Fragments arranged by size:
1039 928 811
NarI GG'CG_CC
Cuts at: 0 2528 2778
Size: 2528 250
NcoI C'CATG_G
Cuts at: 0 933 2778
Size: 933 1845
NgoMIV G'CCGG_C
Cuts at: 0 926 1737 2778
Size: 926 811 1041
Fragments arranged by size:
1041 926 811
NotI GC'GGCC_GC
Cuts at: 0 2172 2778
Size: 2172 606
NspI r_CATG'y
Cuts at: 0 664 1311 1659 2778
Size: 664 647 348 1119
Fragments arranged by size:
1119 664 647 348
PciI A'CATG_T
Cuts at: 0 660 2778
Size: 660 2118
PflMI CCAn_nnn'nTGG
Cuts at: 0 664 2778
Size: 664 2114
PpuMI rG'GwC_Cy
Cuts at: 0 1617 1625 1813 2778
Size: 1617 8 188 965
Fragments arranged by size:
1617 965 188 8
PspXI vC'TCGA_Gb
Cuts at: 0 1951 2778
Size: 1951 827
PstI C_TGCA'G
Cuts at: 0 569 1891 2069 2192 2778
Size: 569 1322 178 123 586
Fragments arranged by size:
1322 586 569 178 123
SacI G_AGCT'C
Cuts at: 0 1271 2605 2680 2720 2778
Size: 1271 1334 75 40 58
Fragments arranged by size:
1334 1271 75 58 40
SacII CC_GC'GG
Cuts at: 0 1521 2178 2778
Size: 1521 657 600
Fragments arranged by size:
1521 657 600
SanDI GG'GwC_CC
Cuts at: 0 1813 2778
Size: 1813 965
SapI GCTCTTCn'nnn_
Cuts at: 0 1276 1819 2778
Size: 1276 543 959
Fragments arranged by size:
1276 959 543
ScaI AGT'ACT
Cuts at: 0 685 2778
Size: 685 2093
SexAI A'CCwGG_T
Cuts at: 0 794 2778
Size: 794 1984
SfcI C'TryA_G
Cuts at: 0 565 1581 1887 2065 2188 2778
Size: 565 1016 306 178 123 590
Fragments arranged by size:
1016 590 565 306 178 123
SfoI GGC'GCC
Cuts at: 0 2529 2778
Size: 2529 249
SgrAI Cr'CCGG_yG
Cuts at: 0 1737 2778
Size: 1737 1041
SmlI C'TyrA_G
Cuts at: 0 1951 2731 2778
Size: 1951 780 47
Fragments arranged by size:
1951 780 47
StuI AGG'CCT
Cuts at: 0 1328 2639 2778
Size: 1328 1311 139
Fragments arranged by size:
1328 1311 139
StyI C'CwwG_G
Cuts at: 0 767 933 2778
Size: 767 166 1845
Fragments arranged by size:
1845 767 166
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 1378 1497 2554 2778
Size: 1378 119 1057 224
Fragments arranged by size:
1378 1057 224 119
TatI w'GTAC_w
Cuts at: 0 683 2561 2778
Size: 683 1878 217
Fragments arranged by size:
1878 683 217
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1278 1310 1371 1403 1805 1837 2556 2588
Size: 1278 32 61 32 402 32 719 32
Cuts at: 2588 2597 2629 2778
Size: 9 32 149
Fragments arranged by size:
1278 719 402 149 61 32 32 32
32 32 9
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 549 2292 2604 2778
Size: 549 1743 312 174
Fragments arranged by size:
1743 549 312 174
XhoI C'TCGA_G
Cuts at: 0 1951 2778
Size: 1951 827
XmnI GAAnn'nnTTC
Cuts at: 0 1610 2778
Size: 1610 1168
Enzymes that do cut:
AarI AccI AcuI AflII AflIII AleI AloI AlwNI
ApoI AvaI BaeI BamHI BanI BanII BbeI BciVI
BglI BglII BmrI BpmI Bpu10I BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI
Bsp1286I BspEI BspMI BsrBI BsrDI BsrFI BsrGI BstAPI
BstBI BstEII BstXI BstYI BstZ17I Bsu36I BtgI BtgZI
BtsI CspCI DraIII EaeI EagI EarI EciI EcoICRI
Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I KasI
MfeI MmeI MscI MslI MspA1I NaeI NarI NcoI
NgoMIV NotI NspI PciI PflMI PpuMI PspXI PstI
SacI SacII SanDI SapI ScaI SexAI SfcI SfoI
SgrAI SmlI StuI StyI TaqII TatI TstI XcmI
XhoI XmnI
Enzymes that do not cut:
AatII Acc65I AclI AfeI AgeI AhdI AlfI ApaI
ApaLI AscI AseI AsiSI AvrII BbsI BbvCI BcgI
BclI BfrBI BlpI Bme1580I BmgBI BmtI BplI BpuEI
BsaI BsaAI BsiWI BspHI BssHII BssSI ClaI DraI
DrdI EcoNI EcoRV FseI FspAI HincII HindIII HpaI
KpnI MluI NdeI NheI NruI NsiI PacI PasI
PfoI PmeI PmlI PpiI PshAI PsiI PspOMI PsrI
PvuI PvuII RsrII SalI SbfI SfiI SgfI SmaI
SnaBI SpeI SphI SrfI SspI SwaI Tth111I XbaI
XmaI ZraI