Restriction map for
pFN21AB5567
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# NO CUT ENZYMES ( 122 enzymes) #
##################################
AatII Acc65I AccI AccII AclI
AgeI AhdI AscI AseI AsnI
Asp718I AspEI AspI AvaI AviII
AvrII BamHI BbeI BbrPI BbvCI
BcgI BciVI BclI BcnI BglI
BlnI BlpI Bpu1102I BseRI BsiEI
BsiWI BsoBI Bsp1407I BspDI BsrBI
BsrFI BsrGI BssHII BssSI Bst1107I
BstBI BstEII BstPI BstUI BstZ17I
CelII Cfr10I ClaI CpoI DraI
DraIII DrdI DsaI EagI Eam1105I
EclXI Eco52I EcoNI EcoO65I EcoRI
EcoT22I FauI FbaI FseI FspI
HincII HindII HindIII HpaI KasI
KpnI KspI MfeI MluI MslI
MunI MvnI NaeI NarI NciI
NcoI NgoMIV NheI NotI NruI
NsiI NspV PacI PaeR7I PflFI
PinAI PmaCI PmeI PmlI PshAI
PshBI Psp1406I PvuI RsrII SacII
SalI SapI SbfI ScaI SexAI
SfiI SfoI SfuI SgrAI SmaI
SnaBI SpeI Sse8387I SspBI SspI
SwaI Tsp45I Tth111I XbaI XhoI
XmaCI XmaI
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# ONE CUT ENZYMES ( 40 enzymes) #
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AcyI 597 GGCGTC G[GA]^CG[CT]C
AflII 1128 CTTAAG C^TTAAG
AflIII 129 ACATGT A^C[GA][CT]GT
Alw44I 926 GTGCAC G^TGCAC
Aor51HI 1616 AGCGCT AGC^GCT
ApaI 1509 GGGCCC GGGCC^C
ApaLI 926 GTGCAC G^TGCAC
Asp700I 85 GAAGCTGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
BalI 721 TGGCCA TGG^CCA
BanI 705 GGCACC G^G[CT][GA]CC
BfrI 1128 CTTAAG C^TTAAG
BglII 4 AGATCT A^GATCT
BsaAI 1324 CACGTA [CT]AC^GT[GA]
BsaBI 641 GATTAAAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaHI 597 GGCGTC G[GA]^CG[CT]C
BsgI 1694 GTGCAG GTGCAG(16/14)
BspHI 849 TCATGA T^CATGA
BspLU11I 129 ACATGT A^CATGT
BspMI 1663 GCAGGT ACCTGC(4/8)
BsrDI 1348 GCAATG GCAATG(2/0)
BstAPI 928 GCACAGCATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstXI 1949 CCAAGAGAATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
EaeI 721 TGGCCA [CT]^GGCC[GA]
Eco47III 1616 AGCGCT AGC^GCT
EcoRV 1355 GATATC GAT^ATC
HgaI 598 GCGTC GACGC(5/10)
Hin1I 597 GGCGTC G[GA]^CG[CT]C
MaeII 1325 ACGT A^CGT
MamI 641 GATTAAAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MluNI 721 TGGCCA TGG^CCA
MscI 721 TGGCCA TGG^CCA
PleI 692 GAGTC GAGTC(4/5)
PpuMI 837 GGGTCCT [GA]G^G[AT]CC[CT]
PstI 1802 CTGCAG CTGCA^G
RcaI 849 TCATGA T^CATGA
SacI 1549 GAGCTC GAGCT^C
SphI 933 GCATGC GCATG^C
TfiI 384 GATTC G^A[AT]TC
XcmI 709 CCATTGAAAGGATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmnI 85 GAAGCTGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 26 enzymes) #
###################################
AccIII 72 TCCGGA T^CCGGA
558 TCCGGA
AfaI 755 GTAC GT^AC
1487 GTAC
AlwNI 1028 CAGTTACTG CAG[ACGT][ACGT][ACGT]^CTG
1592 CAGAGACTG
AocI 1292 CCTCAGG CC^T[ACGT]AGG
1768 CCTCAGG
BfaI 727 CTAG C^TAG
998 CTAG
BsaWI 72 TCCGGA [AT]^CCGG[AT]
558 TCCGGA
BseAI 72 TCCGGA T^CCGGA
558 TCCGGA
BsmBI 599 CGTCTC CGTCTC(1/5)
792 GAGACG
BsmFI 509 GGGAC GGGAC(10/14)
694 GTCCC
BsmI 1167 GAATGC GAATGC(1/-1)
668 GCATTC
BspEI 72 TCCGGA T^CCGGA
558 TCCGGA
BstYI 4 AGATCT [GA]^GATC[CT]
552 AGATCC
Bsu36I 1292 CCTCAGG CC^T[ACGT]AGG
1768 CCTCAGG
Eco81I 1292 CCTCAGG CC^T[ACGT]AGG
1768 CCTCAGG
HaeII 775 GGCGCT [GA]GCGC^[CT]
1616 AGCGCT
HinfI 384 GATTC G^A[ACGT]TC
692 GAGTC
MaeI 727 CTAG C^TAG
998 CTAG
MflI 4 AGATCT [GA]^GATC[CT]
552 AGATCC
MroI 72 TCCGGA T^CCGGA
558 TCCGGA
NdeI 1283 CATATG CA^TATG
1910 CATATG
PflMI 36 CCATGTTATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1894 CCAGGCTATGG
RsaI 755 GTAC GT^AC
1487 GTAC
StuI 785 AGGCCT AGG^CCT
1195 AGGCCT
TspRI 924 CAGTG CA[GC]TG[ACGT][ACGT]^
1433 CAGTG
Van91I 36 CCATGTTATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1894 CCAGGCTATGG
XhoII 4 AGATCT [GA]^GATC[CT]
552 AGATCC
#########################################
# 3 OR MORE CUT ENZYMES ( 70 enzymes) #
#########################################
AciI AcsI AluI AlwI ApoI
AspHI AvaII BanII BbsI BbvI
BmyI BpmI BpuAI BsaI BsaJI
BsiHKAI BsiYI BslI BsmAI Bsp1286I
BsrI BssKI BstF5I BstNI Cac8I
CfoI Cfr13I DdeI DpnII DraII
EarI EcoO109I EcoRII EcoT14I Fnu4HI
FokI HaeIII HapII HhaI HinP1I
HpaII HphI ItaI Ksp632I MaeIII
MboI MboII MnlI MseI MspA1I
MspI MvaI MwoI NdeII NlaIII
NlaIV NspI PvuII Sau3AI Sau96I
ScrFI SfaNI SfcI SmlI StyI
TaqI Tru9I TseI Tsp509I TthHB8I