(Circular) (Six-base) MAPSORT of: pFN21AB5567.seq Check: 8179 from: 1 to: 6721 pFN21AB5567 6721 bp With 182 enzymes: SgfI * February 27, 2009 14:34 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4531 278 Size: 53 83 186 936 2995 2468 Fragments arranged by size: 2995 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 4064 1937 Size: 2127 4594 Acc65I G'GTAC_C Cuts at: 4042 4042 Size: 6721 AclI AA'CG_TT Cuts at: 1563 4848 5221 1563 Size: 3285 373 3063 Fragments arranged by size: 3285 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3045 3194 4784 6168 6436 1632 Size: 1413 149 1590 1384 268 1917 Fragments arranged by size: 1917 1590 1413 1384 268 149 AfeI AGC'GCT Cuts at: 3623 3623 Size: 6721 AflII C'TTAA_G Cuts at: 820 1017 3133 820 Size: 197 2116 4408 Fragments arranged by size: 4408 2116 197 AflIII A'CryG_T Cuts at: 1203 2134 5620 6597 1203 Size: 931 3486 977 1327 Fragments arranged by size: 3486 1327 977 931 AgeI A'CCGG_T Cuts at: 5552 5552 Size: 6721 AhdI GACnn_n'nnGTC Cuts at: 5450 5450 Size: 6721 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6721 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6721 AlwNI CAG_nnn'CTG Cuts at: 3038 3602 4464 6036 3038 Size: 564 862 1572 3723 Fragments arranged by size: 3723 1572 862 564 ApaI G_GGCC'C Cuts at: 3518 3518 Size: 6721 ApaLI G'TGCA_C Cuts at: 2931 4778 5934 2931 Size: 1847 1156 3718 Fragments arranged by size: 3718 1847 1156 ApoI r'AATT_y Cuts at: 1493 3141 3469 3656 3983 4030 4256 4408 6517 Size: 1648 328 187 327 47 226 152 2109 Cuts at: 6517 1493 Size: 1697 Fragments arranged by size: 2109 1697 1648 328 327 226 187 152 47 AseI AT'TA_AT Cuts at: 160 1032 5275 160 Size: 872 4243 1606 Fragments arranged by size: 4243 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6721 AvaI C'yCGr_G Cuts at: 1870 1943 4046 1870 Size: 73 2103 4545 Fragments arranged by size: 4545 2103 73 BamHI G'GATC_C Cuts at: 4051 4418 4051 Size: 367 6354 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2710 4042 5497 618 Size: 325 217 627 923 1332 1455 1842 Fragments arranged by size: 1842 1455 1332 923 627 325 217 BanII G_rGCy'C Cuts at: 1391 2472 3518 3558 4040 1391 Size: 1081 1046 40 482 4072 Fragments arranged by size: 4072 1081 1046 482 40 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2843 3051 3247 3435 928 Size: 971 944 208 196 188 4214 Fragments arranged by size: 4214 971 944 208 196 188 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4912 4946 1903 Size: 34 2975 34 3678 Fragments arranged by size: 3678 2975 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4613 5823 4613 Size: 1210 5511 BclI T'GATC_A Cuts at: 1548 1548 Size: 6721 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 5332 136 Size: 107 122 71 631 605 3660 1525 Fragments arranged by size: 3660 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 2009 6716 2009 Size: 4707 2014 BlpI GC'TnA_GC Cuts at: 4138 4138 Size: 6721 Bme1580I G_kGCm'C Cuts at: 1790 2713 2935 3518 4782 5938 1790 Size: 923 222 583 1264 1156 2573 Fragments arranged by size: 2573 1264 1156 923 583 222 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6721 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3245 4452 5410 Size: 416 53 83 399 24 1828 1207 958 Cuts at: 5410 442 Size: 1753 Fragments arranged by size: 1828 1753 1207 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6721 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 3002 3472 3882 5381 1239 Size: 1763 470 410 1499 2579 Fragments arranged by size: 2579 1763 1499 470 410 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2815 3436 3563 4846 5711 6009 2815 Size: 621 127 1283 865 298 3527 Fragments arranged by size: 3527 1283 865 621 298 127 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2342 2865 3581 5384 882 Size: 617 843 523 716 1803 2219 Fragments arranged by size: 2219 1803 843 716 617 523 BsaAI yAC'GTr Cuts at: 493 1204 3331 4512 493 Size: 711 2127 1181 2702 Fragments arranged by size: 2702 2127 1181 711 BsaBI GATnn'nnATC Cuts at: 2650 6715 2650 Size: 4065 2656 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2603 4528 4910 5545 Size: 53 83 186 936 1070 1925 382 635 Cuts at: 5545 275 Size: 1451 Fragments arranged by size: 1925 1451 1070 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2077 2563 5154 5552 5826 5973 6384 2077 Size: 486 2591 398 274 147 411 2414 Fragments arranged by size: 2591 2414 486 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2529 2559 3710 3740 3849 Size: 30 165 30 1126 30 1151 30 109 Cuts at: 3849 3865 3879 3895 1178 Size: 16 14 16 4004 Fragments arranged by size: 4004 1151 1126 165 109 30 30 30 30 16 16 14 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 1186 Size: 6721 BseYI C'CCAG_C Cuts at: 2783 3053 5924 6420 2783 Size: 270 2871 496 3084 Fragments arranged by size: 3084 2871 496 270 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 3720 1163 Size: 573 1984 4164 Fragments arranged by size: 4164 1984 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 4171 4932 5960 1540 Size: 275 186 2170 761 1028 2301 Fragments arranged by size: 2301 2170 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2128 2935 3558 4782 4867 5938 2128 Size: 807 623 1224 85 1071 2911 Fragments arranged by size: 2911 1224 1071 807 623 85 BsmI GAATG_Cn' Cuts at: 2673 3178 4232 4325 2673 Size: 505 1054 93 5069 Fragments arranged by size: 5069 1054 505 93 BsmBI CGTCTCn'nnnn_ Cuts at: 2610 2791 2610 Size: 181 6540 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2128 2472 2713 2935 3518 3558 4040 Size: 399 338 344 241 222 583 40 482 Cuts at: 4040 4782 4867 5938 1391 Size: 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 583 482 399 344 338 241 222 85 40 BspEI T'CCGG_A Cuts at: 2077 2563 6384 2077 Size: 486 3821 2414 Fragments arranged by size: 3821 2414 486 BspHI T'CATG_A Cuts at: 2854 4610 6340 2854 Size: 1756 1730 3235 Fragments arranged by size: 3235 1756 1730 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3659 4076 844 Size: 2815 417 3489 Fragments arranged by size: 3489 2815 417 BsrBI CCG'CTC Cuts at: 1394 1823 4608 1394 Size: 429 2785 3507 Fragments arranged by size: 3507 2785 429 BsrDI GCAATG_nn' Cuts at: 65 1248 3360 5216 5390 65 Size: 1183 2112 1856 174 1396 Fragments arranged by size: 2112 1856 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 5365 5552 1700 Size: 110 3555 187 2869 Fragments arranged by size: 3555 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6721 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6721 BssSI C'ACGA_G Cuts at: 4781 5793 4781 Size: 1012 5709 BstAPI GCAn_nnn'nTGC Cuts at: 794 2939 794 Size: 2145 4576 BstBI TT'CG_AA Cuts at: 5525 5525 Size: 6721 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6721 BstXI CCAn_nnnn'nTGG Cuts at: 3961 3961 Size: 6721 BstYI r'GATC_y Cuts at: 1972 2009 2557 4051 4418 4803 4820 6272 6358 Size: 37 548 1494 367 385 17 1452 86 Cuts at: 6358 6370 6716 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1494 1452 548 385 367 346 86 37 17 12 Bsu36I CC'TnA_GG Cuts at: 3298 3774 3298 Size: 476 6245 BtgI C'CryG_G Cuts at: 513 1065 513 Size: 552 6169 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5982 Fragments arranged by size: 5982 683 56 BtsI GCAGTG_nn' Cuts at: 4237 5031 5051 4237 Size: 794 20 5907 Fragments arranged by size: 5907 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6721 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6686 DraI TTT'AAA Cuts at: 4025 4025 Size: 6721 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5728 809 Size: 507 4412 1802 Fragments arranged by size: 4412 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2726 4168 5057 Size: 54 1425 50 275 18 896 1442 889 Cuts at: 5057 6686 8 Size: 1629 43 Fragments arranged by size: 1629 1442 1425 896 889 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 4168 1537 Size: 275 2356 4090 Fragments arranged by size: 4090 2356 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2400 2613 3565 3832 4651 1353 Size: 1047 213 952 267 819 3423 Fragments arranged by size: 3423 1047 952 819 267 213 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 5308 5682 5828 387 Size: 1064 138 3719 374 146 1280 Fragments arranged by size: 3719 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 3556 3556 Size: 6721 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 3002 3045 3194 3472 3882 4784 5381 Size: 393 1370 43 149 278 410 902 597 Cuts at: 5381 6168 6436 1239 Size: 787 268 1524 Fragments arranged by size: 1524 1370 902 787 597 410 393 278 268 149 43 EcoO109I rG'GnC_Cy Cuts at: 2843 3514 3515 2843 Size: 671 1 6049 Fragments arranged by size: 6049 671 1 EcoRI G'AATT_C Cuts at: 4030 4408 4030 Size: 378 6343 EcoRV GAT'ATC Cuts at: 3362 3362 Size: 6721 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2393 3876 2393 Size: 1483 5238 FspI TGC'GCA Cuts at: 5227 5227 Size: 6721 HaeII r_GCGC'y Cuts at: 1678 2784 3625 5868 1678 Size: 1106 841 2243 2531 Fragments arranged by size: 2531 2243 1106 841 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2542 2574 3175 3207 3395 Size: 32 135 32 970 32 601 32 188 Cuts at: 3395 3427 3757 3789 3865 3897 5362 5394 5436 Size: 32 330 32 76 32 1465 32 42 Cuts at: 5436 5468 1373 Size: 32 2626 Fragments arranged by size: 2626 1465 970 601 330 188 135 76 42 32 32 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 4065 4311 669 Size: 988 281 2127 246 3079 Fragments arranged by size: 3079 2127 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6721 HpaI GTT'AAC Cuts at: 4311 4311 Size: 6721 KpnI G_GTAC'C Cuts at: 4046 4046 Size: 6721 MfeI C'AATT_G Cuts at: 4320 4320 Size: 6721 MluI A'CGCG_T Cuts at: 6597 6597 Size: 6721 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2130 2222 3389 4013 5835 6019 1305 Size: 825 92 1167 624 1822 184 2007 Fragments arranged by size: 2007 1822 1167 825 624 184 92 MscI TGG'CCA Cuts at: 10 64 1489 1832 2728 10 Size: 54 1425 343 896 4003 Fragments arranged by size: 4003 1425 896 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 4679 5038 5197 518 Size: 648 3513 359 159 2042 Fragments arranged by size: 3513 2042 648 359 159 MspA1I CmG'CkG Cuts at: 1606 3800 3878 3932 4137 4814 5962 6207 1606 Size: 2194 78 54 205 677 1148 245 2120 Fragments arranged by size: 2194 2120 1148 677 245 205 78 54 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 6169 NdeI CA'TA_TG Cuts at: 387 3289 3916 387 Size: 2902 627 3192 Fragments arranged by size: 3192 2902 627 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6721 NotI GC'GGCC_GC Cuts at: 4168 4168 Size: 6721 NspI r_CATG'y Cuts at: 1132 1229 2138 2618 2942 4079 5624 1132 Size: 97 909 480 324 1137 1545 2229 Fragments arranged by size: 2229 1545 1137 909 480 324 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6721 PciI A'CATG_T Cuts at: 2134 5620 2134 Size: 3486 3235 PflMI CCAn_nnn'nTGG Cuts at: 1108 2047 3905 1108 Size: 939 1858 3924 Fragments arranged by size: 3924 1858 939 PmeI GTTT'AAAC Cuts at: 4025 4025 Size: 6721 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4790 4822 6303 6335 4790 Size: 32 1481 32 5176 Fragments arranged by size: 5176 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2843 2843 Size: 6721 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6721 PsiI TTA'TAA Cuts at: 2450 4291 2450 Size: 1841 4880 PspOMI G'GGCC_C Cuts at: 3514 3514 Size: 6721 PstI C_TGCA'G Cuts at: 830 3811 4073 830 Size: 2981 262 3478 Fragments arranged by size: 3478 2981 262 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6721 PvuII CAG'CTG Cuts at: 3800 3878 3932 3800 Size: 78 54 6589 Fragments arranged by size: 6589 78 54 SacI G_AGCT'C Cuts at: 3558 3558 Size: 6721 SalI G'TCGA_C Cuts at: 1936 4063 1936 Size: 2127 4594 SbfI CC_TGCA'GG Cuts at: 4073 4073 Size: 6721 ScaI AGT'ACT Cuts at: 4969 6681 4969 Size: 1712 5009 SfcI C'TryA_G Cuts at: 826 1045 2754 3267 3807 4069 5204 5885 6076 Size: 219 1709 513 540 262 1135 681 191 Cuts at: 6076 826 Size: 1471 Fragments arranged by size: 1709 1471 1135 681 540 513 262 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6721 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6721 SmaI CCC'GGG Cuts at: 1872 4048 1872 Size: 2176 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2794 3133 3451 3578 4825 5726 Size: 197 926 851 339 318 127 1247 901 Cuts at: 5726 5988 820 Size: 262 1553 Fragments arranged by size: 1553 1247 926 901 851 339 318 262 197 127 SnaBI TAC'GTA Cuts at: 493 4512 493 Size: 4019 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6721 SphI G_CATG'C Cuts at: 1132 2942 4079 1132 Size: 1810 1137 3774 Fragments arranged by size: 3774 1810 1137 SspI AAT'ATT Cuts at: 5 52 4645 5 Size: 47 4593 2081 Fragments arranged by size: 4593 2081 47 StuI AGG'CCT Cuts at: 1517 2792 3202 1517 Size: 1275 410 5036 Fragments arranged by size: 5036 1275 410 StyI C'CwwG_G Cuts at: 513 1065 2226 2598 3714 4160 513 Size: 552 1161 372 1116 446 3074 Fragments arranged by size: 3074 1161 1116 552 446 372 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 4748 4765 4918 5103 5548 325 Size: 1255 3168 17 153 185 445 1498 Fragments arranged by size: 3168 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 4967 6679 96 Size: 274 80 33 51 1443 2990 1712 138 Fragments arranged by size: 2990 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2236 2268 6681 6713 2236 Size: 32 4413 32 2244 Fragments arranged by size: 4413 2244 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6721 XbaI T'CTAG_A Cuts at: 4057 4057 Size: 6721 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2721 2721 Size: 6721 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6721 XmaI C'CCGG_G Cuts at: 1870 4046 1870 Size: 2176 4545 XmnI GAAnn'nnTTC Cuts at: 2094 4850 2094 Size: 2756 3965 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4529 276 Size: 53 83 186 936 2995 2468 Fragments arranged by size: 2995 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI EcoRV FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SacI SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AloI AscI AvrII BaeI BbeI BbvCI BfrBI BplI Bpu10I BsiWI BstZ17I DraIII EcoNI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PfoI PmlI PspXI PsrI RsrII SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI