Restriction map for
pFN21AB5562
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# NO CUT ENZYMES ( 119 enzymes) #
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AatII AccI AccII AccIII AclI
AcyI AflII AgeI AhdI Aor51HI
ApaI AscI AseI AsnI Asp700I
AspEI AspI AvaI AviII AvrII
BamHI BbeI BbrPI BbvCI BcgI
BfrI BglII BlnI BsaAI BsaHI
BsaI BseAI BsiWI BsmBI BsmI
BsoBI BspDI BspEI BspHI BssHII
BssSI Bst1107I BstBI BstEII BstPI
BstUI BstXI BstZ17I CfoI ClaI
CpoI DraI DrdI Eam1105I Eco47III
EcoNI EcoO65I EcoRV EcoT22I FseI
FspI HaeII HgaI HhaI Hin1I
HinP1I HindIII HpaI KasI KspI
MaeII MfeI MluI MroI MunI
MvnI NarI NcoI NdeI NheI
NotI NruI NsiI NspV PacI
PaeR7I PflFI PinAI PmaCI PmeI
PmlI PshAI PshBI Psp1406I PvuI
RcaI RsrII SacII SalI SapI
SbfI ScaI SexAI SfiI SfoI
SfuI SgrAI SmaI SnaBI SphI
Sse8387I SwaI TfiI Tth111I XbaI
XhoI XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 47 enzymes) #
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AflIII 2223 ACATGT A^C[GA][CT]GT
Alw44I 637 GTGCAC G^TGCAC
AocI 2260 CCTGAGG CC^T[ACGT]AGG
ApaLI 637 GTGCAC G^TGCAC
BciVI 704 GTATCC GTATCC(6/5)
BcnI 1781 CCCGG CC^[GC]GG
BglI 2172 GCCACAGTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BlpI 843 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 843 GCTGAGC GC^T[ACGT]AGC
BsgI 921 CTGCAC GTGCAG(16/14)
BsiEI 1783 CGGCCG CG[GA][CT]^CG
Bsp1407I 1051 TGTACA T^GTACA
BspLU11I 2223 ACATGT A^CATGT
BsrDI 60 CATTGC GCAATG(2/0)
BsrFI 1785 GCCGGC [GA]^CCGG[CT]
BsrGI 1051 TGTACA T^GTACA
BstAPI 998 GCAAGTTTTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
Bsu36I 2260 CCTGAGG CC^T[ACGT]AGG
CelII 843 GCTGAGC GC^T[ACGT]AGC
Cfr10I 1785 GCCGGC [GA]^CCGG[CT]
DraII 1452 AGGTCCC [GA]G^G[ACGT]CC[CT]
DraIII 904 CACTTCGTG CAC[ACGT][ACGT][ACGT]^GTG
DsaI 1411 CCACGG C^C[GA][CT]GG
EagI 1783 CGGCCG C^GGCCG
EarI 1544 GAAGAG CTCTTC(1/4)
EclXI 1783 CGGCCG C^GGCCG
Eco52I 1783 CGGCCG C^GGCCG
Eco81I 2260 CCTGAGG CC^T[ACGT]AGG
EcoO109I 1452 AGGTCCC [GA]G^G[ACGT]CC[CT]
EcoT14I 1890 CCAAGG C^C[AT][AT]GG
HincII 1073 GTCAAC GT[CT]^[GA]AC
HindII 1073 GTCAAC GT[CT]^[GA]AC
Ksp632I 1544 GAAGAG CTCTTC(1/4)
NaeI 1785 GCCGGC GCC^GGC
NciI 1781 CCCGG CC^[GC]GG
NgoMIV 1785 GCCGGC G^CCGGC
NspI 2223 ACATGT [GA]CATG^[CT]
PpuMI 1452 AGGTCCC [GA]G^G[AT]CC[CT]
PvuII 1492 CAGCTG CAG^CTG
SacI 243 GAGCTC GAGCT^C
SpeI 1036 ACTAGT A^CTAGT
SspBI 1051 TGTACA T^GTACA
SspI 1183 AATATT AAT^ATT
StuI 938 AGGCCT AGG^CCT
StyI 1890 CCAAGG C^C[AT][AT]GG
TaqI 369 TCGA T^CGA
TthHB8I 369 TCGA T^CGA
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# TWO CUT ENZYMES ( 21 enzymes) #
###################################
Acc65I 345 GGTACC G^GTACC
1536 GGTACC
Asp718I 345 GGTACC G^GTACC
1536 GGTACC
AspHI 243 GAGCTC G[AT]GC[AT]^C
637 GTGCAC
BpmI 1719 CTCCAG CTGGAG(16/14)
1474 CTGGAG
BsaBI 187 GATGATGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
1654 GATAGAGATC
BsaWI 1280 TCCGGT [AT]^CCGG[AT]
1286 TCCGGT
BseRI 1716 CTCCTC GAGGAG(10/8)
98 GAGGAG
BsiHKAI 243 GAGCTC G[AT]GC[AT]^C
637 GTGCAC
BsrBI 1923 CCGCTC CCGCTC(-3/-3)
214 GAGCGG
HphI 1131 TCACC GGTGA(8/7)
2084 TCACC
KpnI 345 GGTACC GGTAC^C
1536 GGTACC
MamI 187 GATGATGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
1654 GATAGAGATC
MseI 122 TTAA T^TAA
1252 TTAA
MspA1I 1134 CCGCTG C[AC]G^C[GT]G
1492 CAGCTG
PflMI 979 CCAGAGGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1941 CCAGCAGGTGG
PstI 1844 CTGCAG CTGCA^G
2126 CTGCAG
SmlI 774 CTCAAG C^T[CT][GA]AG
1624 CTTGAG
Tru9I 122 TTAA T^TAA
1252 TTAA
Tsp45I 577 GTGAC ^GT[GC]AC
1591 GTGAC
Van91I 979 CCAGAGGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1941 CCAGCAGGTGG
XcmI 389 CCATCTTACGCATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
598 CCACAGTCTGAATGG
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# 3 OR MORE CUT ENZYMES ( 71 enzymes) #
#########################################
AciI AcsI AfaI AluI AlwI
AlwNI ApoI AvaII BalI BanI
BanII BbsI BbvI BclI BfaI
BmyI BpuAI BsaJI BsiYI BslI
BsmAI BsmFI Bsp1286I BspMI BsrI
BssKI BstF5I BstNI BstYI Cac8I
Cfr13I DdeI DpnII EaeI EcoRI
EcoRII FauI FbaI Fnu4HI FokI
HaeIII HapII HinfI HpaII ItaI
MaeI MaeIII MboI MboII MflI
MluNI MnlI MscI MslI MspI
MvaI MwoI NdeII NlaIII NlaIV
PleI RsaI Sau3AI Sau96I ScrFI
SfaNI SfcI TseI Tsp509I TspRI
XhoII