(Circular) (Six-base) MAPSORT of: pFN21AB5562.seq Check: 6700 from: 1 to: 7066 pFN21AB5562 7066 bp With 182 enzymes: SgfI * October 20, 2009 10:33 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2948 3940 2948 Size: 992 6074 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4876 278 Size: 53 83 186 936 3340 2468 Fragments arranged by size: 3340 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 4409 1937 Size: 2472 4594 Acc65I G'GTAC_C Cuts at: 2350 3541 4387 2350 Size: 1191 846 5029 Fragments arranged by size: 5029 1191 846 AclI AA'CG_TT Cuts at: 1563 5193 5566 1563 Size: 3630 373 3063 Fragments arranged by size: 3630 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2670 3231 4009 5129 6513 6781 1632 Size: 1038 561 778 1120 1384 268 1917 Fragments arranged by size: 1917 1384 1120 1038 778 561 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6869 AflIII A'CryG_T Cuts at: 1203 4228 5965 6942 1203 Size: 3025 1737 977 1327 Fragments arranged by size: 3025 1737 1327 977 AgeI A'CCGG_T Cuts at: 5897 5897 Size: 7066 AhdI GACnn_n'nnGTC Cuts at: 5795 5795 Size: 7066 AleI CACnn'nnGTG Cuts at: 1166 3128 3141 3392 1166 Size: 1962 13 251 4840 Fragments arranged by size: 4840 1962 251 13 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2268 1184 Size: 1084 5982 AlwNI CAG_nnn'CTG Cuts at: 2494 2837 2990 3848 4809 6381 2494 Size: 343 153 858 961 1572 3179 Fragments arranged by size: 3179 1572 961 858 343 153 ApaLI G'TGCA_C Cuts at: 2642 5123 6279 2642 Size: 2481 1156 3429 Fragments arranged by size: 3429 2481 1156 ApoI r'AATT_y Cuts at: 1493 2091 2177 3197 3799 4328 4375 4601 4753 Size: 598 86 1020 602 529 47 226 152 Cuts at: 4753 6862 1493 Size: 2109 1697 Fragments arranged by size: 2109 1697 1020 602 598 529 226 152 86 47 AseI AT'TA_AT Cuts at: 160 1032 5620 160 Size: 872 4588 1606 Fragments arranged by size: 4588 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 7066 AvaI C'yCGr_G Cuts at: 1870 1943 4391 1870 Size: 73 2448 4545 Fragments arranged by size: 4545 2448 73 BamHI G'GATC_C Cuts at: 4396 4763 4396 Size: 367 6699 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2350 3502 3541 4046 4387 Size: 325 217 627 563 1152 39 505 341 Cuts at: 4387 5842 618 Size: 1455 1842 Fragments arranged by size: 1842 1455 1152 627 563 505 341 325 217 39 BanII G_rGCy'C Cuts at: 1391 2252 2780 3491 4385 1391 Size: 861 528 711 894 4072 Fragments arranged by size: 4072 894 861 711 528 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2748 3219 4264 928 Size: 971 849 471 1045 3730 Fragments arranged by size: 3730 1045 971 849 471 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 5257 5291 1903 Size: 34 3320 34 3678 Fragments arranged by size: 3678 3320 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2720 4958 6168 2720 Size: 2238 1210 3618 Fragments arranged by size: 3618 2238 1210 BclI T'GATC_A Cuts at: 1548 2197 2485 2635 1548 Size: 649 288 150 5979 Fragments arranged by size: 5979 649 288 150 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2009 4183 5677 Size: 107 122 71 631 605 337 2174 1494 Cuts at: 5677 136 Size: 1525 Fragments arranged by size: 2174 1525 1494 631 605 337 122 107 71 BglII A'GATC_T Cuts at: 7061 7061 Size: 7066 BlpI GC'TnA_GC Cuts at: 2849 4483 2849 Size: 1634 5432 Bme1580I G_kGCm'C Cuts at: 1790 2368 2646 4051 5127 6283 1790 Size: 578 278 1405 1076 1156 2573 Fragments arranged by size: 2573 1405 1156 1076 578 278 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 7066 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2063 2825 3982 Size: 416 53 83 399 24 646 762 1157 Cuts at: 3982 4797 5755 442 Size: 815 958 1753 Fragments arranged by size: 1753 1157 958 815 762 646 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 7066 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2266 3112 3505 2266 Size: 846 393 5827 Fragments arranged by size: 5827 846 393 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 3500 3709 5726 1239 Size: 2261 209 2017 2579 Fragments arranged by size: 2579 2261 2017 209 Bpu10I CC'TnA_GC Cuts at: 3490 3931 3490 Size: 441 6625 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2764 3650 5191 6056 6354 2764 Size: 886 1541 865 298 3476 Fragments arranged by size: 3476 1541 886 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 5729 882 Size: 617 4230 2219 Fragments arranged by size: 4230 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 4857 493 Size: 711 3653 2702 Fragments arranged by size: 3653 2702 711 BsaBI GATnn'nnATC Cuts at: 2196 3663 7060 2196 Size: 1467 3397 2202 Fragments arranged by size: 3397 2202 1467 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 4873 5255 5890 275 Size: 53 83 186 936 3340 382 635 1451 Fragments arranged by size: 3340 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 3285 3291 5499 5897 6171 6318 6729 3285 Size: 6 2208 398 274 147 411 3622 Fragments arranged by size: 3622 2208 411 398 274 147 6 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2097 2127 3031 3061 3760 Size: 30 165 30 694 30 904 30 699 Cuts at: 3760 3790 4271 4301 1178 Size: 30 481 30 3943 Fragments arranged by size: 3943 904 699 694 481 165 30 30 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2118 3712 1186 Size: 932 1594 4540 Fragments arranged by size: 4540 1594 932 BseYI C'CCAG_C Cuts at: 2953 3945 4294 6269 6765 2953 Size: 992 349 1975 496 3254 Fragments arranged by size: 3254 1975 992 496 349 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2911 1163 Size: 573 1175 5318 Fragments arranged by size: 5318 1175 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3791 4516 5277 6305 1540 Size: 275 186 1790 725 761 1028 2301 Fragments arranged by size: 2301 1790 1028 761 725 275 186 BsiHKAI G_wGCw'C Cuts at: 2252 2646 5127 5212 6283 2252 Size: 394 2481 85 1071 3035 Fragments arranged by size: 3035 2481 1071 394 85 BsmI GAATG_Cn' Cuts at: 4577 4670 4577 Size: 93 6973 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2252 2368 2646 2780 3491 4051 4385 Size: 399 462 116 278 134 711 560 334 Cuts at: 4385 5127 5212 6283 1391 Size: 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 711 560 462 399 334 278 134 116 85 BspEI T'CCGG_A Cuts at: 6729 6729 Size: 7066 BspHI T'CATG_A Cuts at: 4955 6685 4955 Size: 1730 5336 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2948 3025 3940 4421 844 Size: 2104 77 915 481 3489 Fragments arranged by size: 3489 2104 915 481 77 BsrBI CCG'CTC Cuts at: 1394 1823 2221 3930 4953 1394 Size: 429 398 1709 1023 3507 Fragments arranged by size: 3507 1709 1023 429 398 BsrDI GCAATG_nn' Cuts at: 65 1248 2064 5561 5735 65 Size: 1183 816 3497 174 1396 Fragments arranged by size: 3497 1396 1183 816 174 BsrFI r'CCGG_y Cuts at: 1700 1810 3790 5710 5897 1700 Size: 110 1980 1920 187 2869 Fragments arranged by size: 2869 1980 1920 187 110 BsrGI T'GTAC_A Cuts at: 96 3056 96 Size: 2960 4106 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 7066 BssSI C'ACGA_G Cuts at: 5126 6138 5126 Size: 1012 6054 BstAPI GCAn_nnn'nTGC Cuts at: 794 3009 794 Size: 2215 4851 BstBI TT'CG_AA Cuts at: 5870 5870 Size: 7066 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 7066 BstYI r'GATC_y Cuts at: 1972 2716 3664 4100 4396 4763 5148 5165 6617 Size: 744 948 436 296 367 385 17 1452 Cuts at: 6617 6703 6715 7061 1972 Size: 86 12 346 1977 Fragments arranged by size: 1977 1452 948 744 436 385 367 346 296 86 17 12 Bsu36I CC'TnA_GG Cuts at: 4266 4266 Size: 7066 BtgI C'CryG_G Cuts at: 513 1065 2004 3416 513 Size: 552 939 1412 4163 Fragments arranged by size: 4163 1412 939 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 4044 487 Size: 683 56 2818 3509 Fragments arranged by size: 3509 2818 683 56 BtsI GCAGTG_nn' Cuts at: 2923 4582 5376 5396 2923 Size: 1659 794 20 4593 Fragments arranged by size: 4593 1659 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 7066 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 7031 DraI TTT'AAA Cuts at: 4370 4370 Size: 7066 DraIII CAC_nnn'GTG Cuts at: 2914 2914 Size: 7066 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 6073 809 Size: 507 4757 1802 Fragments arranged by size: 4757 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2992 3018 3561 Size: 54 1425 50 275 18 1162 26 543 Cuts at: 3561 3788 4175 4258 4513 5402 7031 8 Size: 227 387 83 255 889 1629 43 Fragments arranged by size: 1629 1425 1162 889 543 387 275 255 227 83 54 50 43 26 18 EagI C'GGCC_G Cuts at: 1537 1812 3788 4513 1537 Size: 275 1976 725 4090 Fragments arranged by size: 4090 1976 725 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3544 4996 1353 Size: 2191 1452 3423 Fragments arranged by size: 3423 2191 1452 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 5653 6027 6173 387 Size: 1064 138 4064 374 146 1280 Fragments arranged by size: 4064 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2250 2250 Size: 7066 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2670 3231 3500 3709 4009 5129 5726 Size: 393 1038 561 269 209 300 1120 597 Cuts at: 5726 6513 6781 1239 Size: 787 268 1524 Fragments arranged by size: 1524 1120 1038 787 597 561 393 300 269 268 209 EcoO109I rG'GnC_Cy Cuts at: 3458 3458 Size: 7066 EcoRI G'AATT_C Cuts at: 2091 3197 4328 4375 4753 2091 Size: 1106 1131 47 378 4404 Fragments arranged by size: 4404 1131 1106 378 47 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2285 2285 Size: 7066 FspI TGC'GCA Cuts at: 5572 5572 Size: 7066 HaeII r_GCGC'y Cuts at: 1678 6213 1678 Size: 4535 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2033 2065 2097 2129 2189 Size: 32 135 32 461 32 32 32 60 Cuts at: 2189 2221 4271 4303 5707 5739 5781 5813 1373 Size: 32 2050 32 1404 32 42 32 2626 Fragments arranged by size: 2626 2050 1404 461 135 60 42 32 32 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3080 4410 4656 669 Size: 988 281 1142 1330 246 3079 Fragments arranged by size: 3079 1330 1142 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 7066 HpaI GTT'AAC Cuts at: 4656 4656 Size: 7066 KpnI G_GTAC'C Cuts at: 2354 3545 4391 2354 Size: 1191 846 5029 Fragments arranged by size: 5029 1191 846 MfeI C'AATT_G Cuts at: 4665 4665 Size: 7066 MluI A'CGCG_T Cuts at: 6942 6942 Size: 7066 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2214 3265 3456 3909 6180 6364 1305 Size: 909 1051 191 453 2271 184 2007 Fragments arranged by size: 2271 2007 1051 909 453 191 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2994 3020 3563 4177 4260 Size: 54 1425 343 1162 26 543 614 83 Cuts at: 4260 10 Size: 2816 Fragments arranged by size: 2816 1425 1162 614 543 343 83 54 26 MslI CAynn'nnrTG Cuts at: 518 1166 2649 2765 3128 3141 3392 5024 5383 Size: 648 1483 116 363 13 251 1632 359 Cuts at: 5383 5542 518 Size: 159 2042 Fragments arranged by size: 2042 1632 1483 648 363 359 251 159 116 13 MspA1I CmG'CkG Cuts at: 1606 3141 3499 4482 5159 6307 6552 1606 Size: 1535 358 983 677 1148 245 2120 Fragments arranged by size: 2120 1535 1148 983 677 358 245 NaeI GCC'GGC Cuts at: 3792 3792 Size: 7066 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 5575 Fragments arranged by size: 5575 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 7066 NgoMIV G'CCGG_C Cuts at: 3790 3790 Size: 7066 NheI G'CTAG_C Cuts at: 1052 1052 Size: 7066 NotI GC'GGCC_GC Cuts at: 4513 4513 Size: 7066 NspI r_CATG'y Cuts at: 1132 1229 4232 4424 5969 1132 Size: 97 3003 192 1545 2229 Fragments arranged by size: 3003 2229 1545 192 97 PasI CC'CwG_GG Cuts at: 1348 3760 1348 Size: 2412 4654 PciI A'CATG_T Cuts at: 4228 5965 4228 Size: 1737 5329 PflMI CCAn_nnn'nTGG Cuts at: 1108 2990 3952 1108 Size: 1882 962 4222 Fragments arranged by size: 4222 1882 962 PfoI T'CCnGG_A Cuts at: 2712 4275 2712 Size: 1563 5503 PmeI GTTT'AAAC Cuts at: 4370 4370 Size: 7066 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3338 3370 5135 5167 6648 6680 3338 Size: 32 1765 32 1481 32 3724 Fragments arranged by size: 3724 1765 1481 32 32 32 PpuMI rG'GwC_Cy Cuts at: 3458 3458 Size: 7066 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 7066 PsiI TTA'TAA Cuts at: 4636 4636 Size: 7066 PstI C_TGCA'G Cuts at: 830 3853 4135 4418 830 Size: 3023 282 283 3478 Fragments arranged by size: 3478 3023 283 282 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 7066 PvuII CAG'CTG Cuts at: 3499 3499 Size: 7066 SacI G_AGCT'C Cuts at: 2252 2252 Size: 7066 SalI G'TCGA_C Cuts at: 1936 4408 1936 Size: 2472 4594 SbfI CC_TGCA'GG Cuts at: 4418 4418 Size: 7066 ScaI AGT'ACT Cuts at: 5314 7026 5314 Size: 1712 5354 SfcI C'TryA_G Cuts at: 826 1045 2359 3849 4131 4414 5549 6230 6421 Size: 219 1314 1490 282 283 1135 681 191 Cuts at: 6421 826 Size: 1471 Fragments arranged by size: 1490 1471 1314 1135 681 283 282 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 7066 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 7066 SmaI CCC'GGG Cuts at: 1872 4393 1872 Size: 2521 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2779 3629 5170 6071 6333 820 Size: 197 926 836 850 1541 901 262 1553 Fragments arranged by size: 1553 1541 926 901 850 836 262 197 SnaBI TAC'GTA Cuts at: 493 4857 493 Size: 4364 2702 SpeI A'CTAG_T Cuts at: 152 3041 152 Size: 2889 4177 SphI G_CATG'C Cuts at: 1132 4424 1132 Size: 3292 3774 SspI AAT'ATT Cuts at: 5 52 3190 4990 5 Size: 47 3138 1800 2081 Fragments arranged by size: 3138 2081 1800 47 StuI AGG'CCT Cuts at: 1517 2945 1517 Size: 1428 5638 StyI C'CwwG_G Cuts at: 513 1065 2004 3895 4505 513 Size: 552 939 1891 610 3074 Fragments arranged by size: 3074 1891 939 610 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2661 3520 3758 3908 4157 5093 5110 Size: 1255 1081 859 238 150 249 936 17 Cuts at: 5110 5263 5448 5893 325 Size: 153 185 445 1498 Fragments arranged by size: 1498 1255 1081 936 859 445 249 238 185 153 150 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3056 3369 5312 Size: 274 80 33 51 1443 1079 313 1943 Cuts at: 5312 7024 96 Size: 1712 138 Fragments arranged by size: 1943 1712 1443 1079 313 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2408 2440 3760 3792 7026 7058 2408 Size: 32 1320 32 3234 32 2416 Fragments arranged by size: 3234 2416 1320 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 7066 XbaI T'CTAG_A Cuts at: 4402 4402 Size: 7066 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2401 2610 2401 Size: 209 6857 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 7066 XmaI C'CCGG_G Cuts at: 1870 4391 1870 Size: 2521 4545 XmnI GAAnn'nnTTC Cuts at: 5195 5195 Size: 7066 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4874 276 Size: 53 83 186 936 3340 2468 Fragments arranged by size: 3340 2468 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PstI PvuI PvuII SacI SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI BfrBI BsiWI BsmBI BstXI BstZ17I EcoNI EcoRV FseI FspAI KasI NarI NruI NsiI PacI PmlI PspOMI PspXI PsrI RsrII SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI