(Circular) (Six-base) MAPSORT of: pFN21AB5555.seq Check: 7110 from: 1 to: 6061 pFN21AB5555 6061 bp With 182 enzymes: SgfI * January 16, 2009 15:25 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3871 278 Size: 53 83 186 936 2335 2468 Fragments arranged by size: 2468 2335 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3404 1937 Size: 1467 4594 Acc65I G'GTAC_C Cuts at: 3382 3382 Size: 6061 AclI AA'CG_TT Cuts at: 1563 4188 4561 1563 Size: 2625 373 3063 Fragments arranged by size: 3063 2625 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 4124 5508 5776 1632 Size: 2492 1384 268 1917 Fragments arranged by size: 2492 1917 1384 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5864 AflIII A'CryG_T Cuts at: 1203 4960 5937 1203 Size: 3757 977 1327 Fragments arranged by size: 3757 1327 977 AgeI A'CCGG_T Cuts at: 4892 4892 Size: 6061 AhdI GACnn_n'nnGTC Cuts at: 2050 2814 4790 2050 Size: 764 1976 3321 Fragments arranged by size: 3321 1976 764 AleI CACnn'nnGTG Cuts at: 1166 2558 3332 1166 Size: 1392 774 3895 Fragments arranged by size: 3895 1392 774 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6061 AlwNI CAG_nnn'CTG Cuts at: 2800 2964 3109 3804 5376 2800 Size: 164 145 695 1572 3485 Fragments arranged by size: 3485 1572 695 164 145 ApaI G_GGCC'C Cuts at: 2807 3019 2807 Size: 212 5849 ApaLI G'TGCA_C Cuts at: 3056 4118 5274 3056 Size: 1062 1156 3843 Fragments arranged by size: 3843 1156 1062 ApoI r'AATT_y Cuts at: 1493 2707 3080 3370 3596 3748 5857 1493 Size: 1214 373 290 226 152 2109 1697 Fragments arranged by size: 2109 1697 1214 373 290 226 152 AseI AT'TA_AT Cuts at: 160 1032 4615 160 Size: 872 3583 1606 Fragments arranged by size: 3583 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6061 AvaI C'yCGr_G Cuts at: 1870 1943 2113 3386 1870 Size: 73 170 1273 4545 Fragments arranged by size: 4545 1273 170 73 BamHI G'GATC_C Cuts at: 2228 3391 3758 2228 Size: 1163 367 4531 Fragments arranged by size: 4531 1163 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3382 4837 618 Size: 325 217 627 1595 1455 1842 Fragments arranged by size: 1842 1595 1455 627 325 217 BanII G_rGCy'C Cuts at: 1391 2807 3019 3380 1391 Size: 1416 212 361 4072 Fragments arranged by size: 4072 1416 361 212 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2664 928 Size: 971 765 4325 Fragments arranged by size: 4325 971 765 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 2090 2124 4252 4286 1903 Size: 34 153 34 2128 34 3678 Fragments arranged by size: 3678 2128 153 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3953 5163 3953 Size: 1210 4851 BclI T'GATC_A Cuts at: 1548 1548 Size: 6061 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4672 136 Size: 107 122 71 631 605 3000 1525 Fragments arranged by size: 3000 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 6056 6056 Size: 6061 BlpI GC'TnA_GC Cuts at: 3008 3478 3008 Size: 470 5591 Bme1580I G_kGCm'C Cuts at: 1790 2746 2807 3019 3060 4122 5278 1790 Size: 956 61 212 41 1062 1156 2573 Fragments arranged by size: 2573 1156 1062 956 212 61 41 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6061 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3792 4750 442 Size: 416 53 83 399 24 2375 958 1753 Fragments arranged by size: 2375 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6061 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2893 2893 Size: 6061 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2450 2888 3226 4721 1239 Size: 1211 438 338 1495 2579 Fragments arranged by size: 2579 1495 1211 438 338 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4186 5051 5349 4186 Size: 865 298 4898 Fragments arranged by size: 4898 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4724 882 Size: 617 3225 2219 Fragments arranged by size: 3225 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3334 3852 493 Size: 711 2130 518 2702 Fragments arranged by size: 2702 2130 711 518 BsaBI GATnn'nnATC Cuts at: 2462 2790 6055 2462 Size: 328 3265 2468 Fragments arranged by size: 3265 2468 328 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3868 4250 4885 275 Size: 53 83 186 936 2335 382 635 1451 Fragments arranged by size: 2335 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2242 4494 4892 5166 5313 5724 2242 Size: 2252 398 274 147 411 2579 Fragments arranged by size: 2579 2252 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2015 2045 3260 3290 1178 Size: 30 165 30 612 30 1215 30 3949 Fragments arranged by size: 3949 1215 612 165 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2334 2383 2700 1186 Size: 1148 49 317 4547 Fragments arranged by size: 4547 1148 317 49 BseYI C'CCAG_C Cuts at: 2778 2964 5264 5760 2778 Size: 186 2300 496 3079 Fragments arranged by size: 3079 2300 496 186 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 5488 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3126 3511 4272 5300 1540 Size: 275 186 1125 385 761 1028 2301 Fragments arranged by size: 2301 1125 1028 761 385 275 186 BsiHKAI G_wGCw'C Cuts at: 3060 4122 4207 5278 3060 Size: 1062 85 1071 3843 Fragments arranged by size: 3843 1071 1062 85 BsiWI C'GTAC_G Cuts at: 2070 2070 Size: 6061 BsmI GAATG_Cn' Cuts at: 3572 3665 3572 Size: 93 5968 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2746 2807 3019 3060 3380 4122 4207 Size: 399 956 61 212 41 320 742 85 Cuts at: 4207 5278 1391 Size: 1071 2174 Fragments arranged by size: 2174 1071 956 742 399 320 212 85 61 41 BspEI T'CCGG_A Cuts at: 5724 5724 Size: 6061 BspHI T'CATG_A Cuts at: 3950 5680 3950 Size: 1730 4331 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3416 844 Size: 2572 3489 BsrBI CCG'CTC Cuts at: 1394 1823 2078 2974 3948 1394 Size: 429 255 896 974 3507 Fragments arranged by size: 3507 974 896 429 255 BsrDI GCAATG_nn' Cuts at: 65 1248 2429 3096 4556 4730 65 Size: 1183 1181 667 1460 174 1396 Fragments arranged by size: 1460 1396 1183 1181 667 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4705 4892 1700 Size: 110 2895 187 2869 Fragments arranged by size: 2895 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 3138 96 Size: 3042 3019 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6061 BssSI C'ACGA_G Cuts at: 4121 5133 4121 Size: 1012 5049 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6061 BstBI TT'CG_AA Cuts at: 4865 4865 Size: 6061 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6061 BstXI CCAn_nnnn'nTGG Cuts at: 2225 2225 Size: 6061 BstYI r'GATC_y Cuts at: 1972 2228 2785 2796 2844 3391 3758 4143 4160 Size: 256 557 11 48 547 367 385 17 Cuts at: 4160 5612 5698 5710 6056 1972 Size: 1452 86 12 346 1977 Fragments arranged by size: 1977 1452 557 547 385 367 346 256 86 48 17 12 11 Bsu36I CC'TnA_GG Cuts at: 2585 2585 Size: 6061 BtgI C'CryG_G Cuts at: 513 1065 2004 3091 3282 513 Size: 552 939 1087 191 3292 Fragments arranged by size: 3292 1087 939 552 191 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5322 Fragments arranged by size: 5322 683 56 BtsI GCAGTG_nn' Cuts at: 2597 3577 4371 4391 2597 Size: 980 794 20 4267 Fragments arranged by size: 4267 980 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6061 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6026 DraI TTT'AAA Cuts at: 3365 3365 Size: 6061 DraIII CAC_nnn'GTG Cuts at: 2400 2400 Size: 6061 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 3130 5068 809 Size: 507 1814 1938 1802 Fragments arranged by size: 1938 1814 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2955 3508 4397 Size: 54 1425 50 275 18 1125 553 889 Cuts at: 4397 6026 8 Size: 1629 43 Fragments arranged by size: 1629 1425 1125 889 553 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3508 1537 Size: 275 1696 4090 Fragments arranged by size: 4090 1696 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2677 3991 1353 Size: 1324 1314 3423 Fragments arranged by size: 3423 1324 1314 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2298 4648 5022 5168 387 Size: 1064 138 709 2350 374 146 1280 Fragments arranged by size: 2350 1280 1064 709 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2450 2888 3226 4124 4721 5508 5776 Size: 393 818 438 338 898 597 787 268 Cuts at: 5776 1239 Size: 1524 Fragments arranged by size: 1524 898 818 787 597 438 393 338 268 EcoO109I rG'GnC_Cy Cuts at: 3016 3016 Size: 6061 EcoRI G'AATT_C Cuts at: 2707 3370 3748 2707 Size: 663 378 5020 Fragments arranged by size: 5020 663 378 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2542 2706 3090 3217 2542 Size: 164 384 127 5386 Fragments arranged by size: 5386 384 164 127 FspI TGC'GCA Cuts at: 4567 4567 Size: 6061 HaeII r_GCGC'y Cuts at: 1678 2889 2965 5208 1678 Size: 1211 76 2243 2531 Fragments arranged by size: 2531 2243 1211 76 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2015 2036 2047 2068 2364 Size: 32 135 32 443 21 11 21 296 Cuts at: 2364 2396 2621 2634 2653 2666 2800 2832 4702 Size: 32 225 13 19 13 134 32 1870 Cuts at: 4702 4734 4776 4808 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 1870 443 296 225 135 134 42 32 32 32 32 32 32 21 21 19 13 13 11 HincII GTy'rAC Cuts at: 669 1657 1938 2197 3405 3651 669 Size: 988 281 259 1208 246 3079 Fragments arranged by size: 3079 1208 988 281 259 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6061 HpaI GTT'AAC Cuts at: 3651 3651 Size: 6061 KpnI G_GTAC'C Cuts at: 3386 3386 Size: 6061 MfeI C'AATT_G Cuts at: 3660 3660 Size: 6061 MluI A'CGCG_T Cuts at: 5937 5937 Size: 6061 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2319 2904 3146 5175 5359 1305 Size: 1014 585 242 2029 184 2007 Fragments arranged by size: 2029 2007 1014 585 242 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2957 10 Size: 54 1425 343 1125 3114 Fragments arranged by size: 3114 1425 1125 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2223 2558 3332 4019 4378 4537 518 Size: 648 1057 335 774 687 359 159 2042 Fragments arranged by size: 2042 1057 774 687 648 359 335 159 MspA1I CmG'CkG Cuts at: 1606 2851 2898 3477 4154 5302 5547 1606 Size: 1245 47 579 677 1148 245 2120 Fragments arranged by size: 2120 1245 1148 677 579 245 47 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 4570 Fragments arranged by size: 4570 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 6061 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6061 NotI GC'GGCC_GC Cuts at: 3508 3508 Size: 6061 NspI r_CATG'y Cuts at: 1132 1229 2417 3169 3419 4964 1132 Size: 97 1188 752 250 1545 2229 Fragments arranged by size: 2229 1545 1188 752 250 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6061 PciI A'CATG_T Cuts at: 4960 4960 Size: 6061 PflMI CCAn_nnn'nTGG Cuts at: 1108 2400 2488 2964 1108 Size: 1292 88 476 4205 Fragments arranged by size: 4205 1292 476 88 PfoI T'CCnGG_A Cuts at: 2865 2865 Size: 6061 PmeI GTTT'AAAC Cuts at: 3365 3365 Size: 6061 PmlI CAC'GTG Cuts at: 3334 3334 Size: 6061 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4130 4162 5643 5675 4130 Size: 32 1481 32 4516 Fragments arranged by size: 4516 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6061 PsiI TTA'TAA Cuts at: 3631 3631 Size: 6061 PspOMI G'GGCC_C Cuts at: 2803 3015 2803 Size: 212 5849 PstI C_TGCA'G Cuts at: 830 2255 3114 3413 830 Size: 1425 859 299 3478 Fragments arranged by size: 3478 1425 859 299 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6061 PvuII CAG'CTG Cuts at: 2851 2898 2851 Size: 47 6014 SalI G'TCGA_C Cuts at: 1936 3403 1936 Size: 1467 4594 SbfI CC_TGCA'GG Cuts at: 3413 3413 Size: 6061 ScaI AGT'ACT Cuts at: 4309 6021 4309 Size: 1712 4349 SfcI C'TryA_G Cuts at: 826 1045 2251 3110 3409 4544 5225 5416 826 Size: 219 1206 859 299 1135 681 191 1471 Fragments arranged by size: 1471 1206 1135 859 681 299 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6061 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6061 SmaI CCC'GGG Cuts at: 1872 3388 1872 Size: 1516 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 4165 5066 5328 820 Size: 197 926 2222 901 262 1553 Fragments arranged by size: 2222 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3852 493 Size: 3359 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6061 SphI G_CATG'C Cuts at: 1132 3419 1132 Size: 2287 3774 SspI AAT'ATT Cuts at: 5 52 3985 5 Size: 47 3933 2081 Fragments arranged by size: 3933 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6061 StyI C'CwwG_G Cuts at: 513 1065 2004 2094 2734 3500 513 Size: 552 939 90 640 766 3074 Fragments arranged by size: 3074 939 766 640 552 90 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3140 3344 4088 4105 4258 4443 4888 Size: 1255 1560 204 744 17 153 185 445 Cuts at: 4888 325 Size: 1498 Fragments arranged by size: 1560 1498 1255 744 445 204 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2448 3138 4307 Size: 274 80 33 51 1443 471 690 1169 Cuts at: 4307 6019 96 Size: 1712 138 Fragments arranged by size: 1712 1443 1169 690 471 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 6021 6053 6021 Size: 32 6029 Tth111I GACn'n_nGTC Cuts at: 1316 2675 3127 1316 Size: 1359 452 4250 Fragments arranged by size: 4250 1359 452 XbaI T'CTAG_A Cuts at: 3397 3397 Size: 6061 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2169 2489 2169 Size: 320 5741 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6061 XmaI C'CCGG_G Cuts at: 1870 3386 1870 Size: 1516 4545 XmnI GAAnn'nnTTC Cuts at: 4190 4190 Size: 6061 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3869 276 Size: 53 83 186 936 2335 2468 Fragments arranged by size: 2468 2335 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsiWI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PmlI PpiI PshAI PsiI PspOMI PstI PvuI PvuII SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI AscI AvrII BaeI BbeI BbvCI BfrBI Bpu10I BsmBI BstZ17I EcoICRI EcoNI EcoRV FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PpuMI PspXI PsrI RsrII SacI SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI