Restriction map for
pFN21AB5555
(Linear) (Six-base) MAPSORT of: KIBB5555.seq Check: 5782 from: 1 to: 1356
KIBB5555.orf
With 182 enzymes: SgfI *
February 28, 2007 11:27 ..
AhdI GACnn_n'nnGTC
Cuts at: 0 45 809 1356
Size: 45 764 547
Fragments arranged by size:
764 547 45
AleI CACnn'nnGTG
Cuts at: 0 553 1327 1356
Size: 553 774 29
Fragments arranged by size:
774 553 29
AlwNI CAG_nnn'CTG
Cuts at: 0 795 959 1104 1356
Size: 795 164 145 252
Fragments arranged by size:
795 252 164 145
ApaI G_GGCC'C
Cuts at: 0 802 1014 1356
Size: 802 212 342
Fragments arranged by size:
802 342 212
ApaLI G'TGCA_C
Cuts at: 0 1051 1356
Size: 1051 305
ApoI r'AATT_y
Cuts at: 0 702 1075 1356
Size: 702 373 281
Fragments arranged by size:
702 373 281
AvaI C'yCGr_G
Cuts at: 0 108 1356
Size: 108 1248
BamHI G'GATC_C
Cuts at: 0 223 1356
Size: 223 1133
BanII G_rGCy'C
Cuts at: 0 802 1014 1356
Size: 802 212 342
Fragments arranged by size:
802 342 212
BbsI GAAGACnn'nnnn_
Cuts at: 0 659 1356
Size: 659 697
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 85 119 1356
Size: 85 34 1237
Fragments arranged by size:
1237 85 34
BlpI GC'TnA_GC
Cuts at: 0 1003 1356
Size: 1003 353
Bme1580I G_kGCm'C
Cuts at: 0 741 802 1014 1055 1356
Size: 741 61 212 41 301
Fragments arranged by size:
741 301 212 61 41
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 888 1356
Size: 888 468
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 445 883 1221 1356
Size: 445 438 338 135
Fragments arranged by size:
445 438 338 135
BsaAI yAC'GTr
Cuts at: 0 1329 1356
Size: 1329 27
BsaBI GATnn'nnATC
Cuts at: 0 457 785 1356
Size: 457 328 571
Fragments arranged by size:
571 457 328
BsaWI w'CCGG_w
Cuts at: 0 237 1356
Size: 237 1119
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 10 40 1255 1285 1356
Size: 10 30 1215 30 71
Fragments arranged by size:
1215 71 30 30 10
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 329 378 695 1356
Size: 329 49 317 661
Fragments arranged by size:
661 329 317 49
BseYI C'CCAG_C
Cuts at: 0 773 959 1356
Size: 773 186 397
Fragments arranged by size:
773 397 186
BsiEI CG_ry'CG
Cuts at: 0 1121 1356
Size: 1121 235
BsiHKAI G_wGCw'C
Cuts at: 0 1055 1356
Size: 1055 301
BsiWI C'GTAC_G
Cuts at: 0 65 1356
Size: 65 1291
Bsp1286I G_dGCh'C
Cuts at: 0 741 802 1014 1055 1356
Size: 741 61 212 41 301
Fragments arranged by size:
741 301 212 61 41
BsrBI CCG'CTC
Cuts at: 0 73 969 1356
Size: 73 896 387
Fragments arranged by size:
896 387 73
BsrDI GCAATG_nn'
Cuts at: 0 424 1091 1356
Size: 424 667 265
Fragments arranged by size:
667 424 265
BsrGI T'GTAC_A
Cuts at: 0 1133 1356
Size: 1133 223
BstXI CCAn_nnnn'nTGG
Cuts at: 0 220 1356
Size: 220 1136
BstYI r'GATC_y
Cuts at: 0 223 780 791 839 1356
Size: 223 557 11 48 517
Fragments arranged by size:
557 517 223 48 11
Bsu36I CC'TnA_GG
Cuts at: 0 580 1356
Size: 580 776
BtgI C'CryG_G
Cuts at: 0 1086 1277 1356
Size: 1086 191 79
Fragments arranged by size:
1086 191 79
BtsI GCAGTG_nn'
Cuts at: 0 592 1356
Size: 592 764
DraIII CAC_nnn'GTG
Cuts at: 0 395 1356
Size: 395 961
DrdI GACnn_nn'nnGTC
Cuts at: 0 1125 1356
Size: 1125 231
EaeI y'GGCC_r
Cuts at: 0 950 1356
Size: 950 406
EarI CTCTTCn'nnn_
Cuts at: 0 672 1356
Size: 672 684
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 293 1356
Size: 293 1063
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 445 883 1221 1356
Size: 445 438 338 135
Fragments arranged by size:
445 438 338 135
EcoO109I rG'GnC_Cy
Cuts at: 0 1011 1356
Size: 1011 345
EcoRI G'AATT_C
Cuts at: 0 702 1356
Size: 702 654
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 537 701 1085 1212 1356
Size: 537 164 384 127 144
Fragments arranged by size:
537 384 164 144 127
HaeII r_GCGC'y
Cuts at: 0 884 960 1356
Size: 884 76 396
Fragments arranged by size:
884 396 76
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 10 31 42 63 359 391 616 629
Size: 10 21 11 21 296 32 225 13
Cuts at: 629 648 661 795 827 1356
Size: 19 13 134 32 529
Fragments arranged by size:
529 296 225 134 32 32 21 21
19 13 13 11 10
HincII GTy'rAC
Cuts at: 0 192 1356
Size: 192 1164
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 314 899 1141 1356
Size: 314 585 242 215
Fragments arranged by size:
585 314 242 215
MscI TGG'CCA
Cuts at: 0 952 1356
Size: 952 404
MslI CAynn'nnrTG
Cuts at: 0 218 553 1327 1356
Size: 218 335 774 29
Fragments arranged by size:
774 335 218 29
MspA1I CmG'CkG
Cuts at: 0 846 893 1356
Size: 846 47 463
Fragments arranged by size:
846 463 47
NspI r_CATG'y
Cuts at: 0 412 1164 1356
Size: 412 752 192
Fragments arranged by size:
752 412 192
PflMI CCAn_nnn'nTGG
Cuts at: 0 395 483 959 1356
Size: 395 88 476 397
Fragments arranged by size:
476 397 395 88
PfoI T'CCnGG_A
Cuts at: 0 860 1356
Size: 860 496
PmlI CAC'GTG
Cuts at: 0 1329 1356
Size: 1329 27
PspOMI G'GGCC_C
Cuts at: 0 798 1010 1356
Size: 798 212 346
Fragments arranged by size:
798 346 212
PstI C_TGCA'G
Cuts at: 0 250 1109 1356
Size: 250 859 247
Fragments arranged by size:
859 250 247
PvuII CAG'CTG
Cuts at: 0 846 893 1356
Size: 846 47 463
Fragments arranged by size:
846 463 47
SfcI C'TryA_G
Cuts at: 0 246 1105 1356
Size: 246 859 251
Fragments arranged by size:
859 251 246
StyI C'CwwG_G
Cuts at: 0 89 729 1356
Size: 89 640 627
Fragments arranged by size:
640 627 89
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 1135 1339 1356
Size: 1135 204 17
Fragments arranged by size:
1135 204 17
TatI w'GTAC_w
Cuts at: 0 443 1133 1356
Size: 443 690 223
Fragments arranged by size:
690 443 223
Tth111I GACn'n_nGTC
Cuts at: 0 670 1122 1356
Size: 670 452 234
Fragments arranged by size:
670 452 234
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 164 484 1356
Size: 164 320 872
Fragments arranged by size:
872 320 164
Enzymes that do cut:
AhdI AleI AlwNI ApaI ApaLI ApoI AvaI BamHI
BanII BbsI BcgI BlpI Bme1580I BplI BpmI BsaAI
BsaBI BsaWI BsaXI BseRI BseYI BsiEI BsiHKAI BsiWI
Bsp1286I BsrBI BsrDI BsrGI BstXI BstYI Bsu36I BtgI
BtsI DraIII DrdI EaeI EarI EciI Eco57MI EcoO109I
EcoRI FalI HaeII Hin4I HincII MmeI MscI MslI
MspA1I NspI PflMI PfoI PmlI PspOMI PstI PvuII
SfcI StyI TaqII TatI Tth111I XcmI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AlfI AloI AscI AseI AsiSI AvrII
BaeI BanI BbeI BbvCI BciVI BclI BfrBI BglI
BglII BmgBI BmrI BmtI Bpu10I BpuEI BsaI BsaHI
BsgI BsmI BsmBI BspEI BspHI BspMI BsrFI BssHII
BssSI BstAPI BstBI BstEII BstZ17I BtgZI ClaI CspCI
DraI EagI EcoICRI EcoNI EcoRV FseI FspI FspAI
HindIII HpaI KasI KpnI MfeI MluI NaeI NarI
NcoI NdeI NgoMIV NheI NotI NruI NsiI PacI
PasI PciI PmeI PpiI PpuMI PshAI PsiI PspXI
PsrI PvuI RsrII SacI SacII SalI SanDI SapI
SbfI ScaI SexAI SfiI SfoI SgfI SgrAI SmaI
SmlI SnaBI SpeI SphI SrfI SspI StuI SwaI
TstI XbaI XhoI XmaI XmnI ZraI