(Circular) (Six-base) MAPSORT of: pFN21AB5508.seq Check: 7422 from: 1 to: 6334 pFN21AB5508 6334 bp With 182 enzymes: SgfI * February 27, 2009 14:34 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4144 278 Size: 53 83 186 936 2608 2468 Fragments arranged by size: 2608 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2700 2760 3677 1937 Size: 763 60 917 4594 Fragments arranged by size: 4594 917 763 60 Acc65I G'GTAC_C Cuts at: 3655 3655 Size: 6334 AclI AA'CG_TT Cuts at: 1563 4461 4834 1563 Size: 2898 373 3063 Fragments arranged by size: 3063 2898 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2039 2966 3146 4397 5781 6049 1632 Size: 407 927 180 1251 1384 268 1917 Fragments arranged by size: 1917 1384 1251 927 407 268 180 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6137 AflIII A'CryG_T Cuts at: 1203 5233 6210 1203 Size: 4030 977 1327 Fragments arranged by size: 4030 1327 977 AgeI A'CCGG_T Cuts at: 5165 5165 Size: 6334 AhdI GACnn_n'nnGTC Cuts at: 5063 5063 Size: 6334 AleI CACnn'nnGTG Cuts at: 1166 3221 1166 Size: 2055 4279 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2117 1184 Size: 933 5401 AlwNI CAG_nnn'CTG Cuts at: 2236 2440 2689 2857 3088 4077 5649 2236 Size: 204 249 168 231 989 1572 2921 Fragments arranged by size: 2921 1572 989 249 231 204 168 ApaI G_GGCC'C Cuts at: 2908 3266 2908 Size: 358 5976 ApaLI G'TGCA_C Cuts at: 4391 5547 4391 Size: 1156 5178 ApoI r'AATT_y Cuts at: 1493 3643 3869 4021 6130 1493 Size: 2150 226 152 2109 1697 Fragments arranged by size: 2150 2109 1697 226 152 AseI AT'TA_AT Cuts at: 160 1032 4888 160 Size: 872 3856 1606 Fragments arranged by size: 3856 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6334 AvaI C'yCGr_G Cuts at: 1870 1943 2048 2467 2744 3659 1870 Size: 73 105 419 277 915 4545 Fragments arranged by size: 4545 915 419 277 105 73 BamHI G'GATC_C Cuts at: 3664 4031 3664 Size: 367 5967 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2251 2324 2483 2808 3251 Size: 325 217 627 464 73 159 325 443 Cuts at: 3251 3385 3595 3655 5110 618 Size: 134 210 60 1455 1842 Fragments arranged by size: 1842 1455 627 464 443 325 325 217 210 159 134 73 60 BanII G_rGCy'C Cuts at: 1391 2280 2908 3266 3317 3626 3653 1391 Size: 889 628 358 51 309 27 4072 Fragments arranged by size: 4072 889 628 358 309 51 27 BbeI G_GCGC'C Cuts at: 2812 3389 2812 Size: 577 5757 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 5363 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 2086 2120 2326 2360 4525 4559 1903 Size: 34 149 34 206 34 2165 34 3678 Fragments arranged by size: 3678 2165 206 149 34 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2483 4226 5436 2483 Size: 1743 1210 3381 Fragments arranged by size: 3381 1743 1210 BclI T'GATC_A Cuts at: 1548 1548 Size: 6334 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 3621 4945 136 Size: 107 122 71 631 605 1949 1324 1525 Fragments arranged by size: 1949 1525 1324 631 605 122 107 71 BglII A'GATC_T Cuts at: 2589 3369 6329 2589 Size: 780 2960 2594 Fragments arranged by size: 2960 2594 780 BlpI GC'TnA_GC Cuts at: 3404 3479 3751 3404 Size: 75 272 5987 Fragments arranged by size: 5987 272 75 Bme1580I G_kGCm'C Cuts at: 1790 2035 2256 2327 2334 2422 2741 2799 2908 Size: 245 221 71 7 88 319 58 109 Cuts at: 2908 3254 3266 4395 5551 1790 Size: 346 12 1129 1156 2573 Fragments arranged by size: 2573 1156 1129 346 319 245 221 109 88 71 58 12 7 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6334 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 4065 5023 442 Size: 416 53 83 399 24 2648 958 1753 Fragments arranged by size: 2648 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6334 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2977 3331 2977 Size: 354 5980 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2132 2174 2261 2462 2606 2843 3161 3230 Size: 893 42 87 201 144 237 318 69 Cuts at: 3230 4994 1239 Size: 1764 2579 Fragments arranged by size: 2579 1764 893 318 237 201 144 87 69 42 Bpu10I CC'TnA_GC Cuts at: 2274 2274 Size: 6334 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2063 4459 5324 5622 2063 Size: 2396 865 298 2775 Fragments arranged by size: 2775 2396 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4997 882 Size: 617 3498 2219 Fragments arranged by size: 3498 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 4125 493 Size: 711 2921 2702 Fragments arranged by size: 2921 2702 711 BsaBI GATnn'nnATC Cuts at: 6328 6328 Size: 6334 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2809 3386 3613 4141 Size: 53 83 186 936 1276 577 227 528 Cuts at: 4141 4523 5158 275 Size: 382 635 1451 Fragments arranged by size: 1451 1276 936 635 577 528 382 227 186 83 53 BsaWI w'CCGG_w Cuts at: 4767 5165 5439 5586 5997 4767 Size: 398 274 147 411 5104 Fragments arranged by size: 5104 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2753 2783 3608 3638 1178 Size: 30 165 30 1350 30 825 30 3874 Fragments arranged by size: 3874 1350 825 165 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2129 2780 2840 3218 3323 1186 Size: 943 651 60 378 105 4197 Fragments arranged by size: 4197 943 651 378 105 60 BseYI C'CCAG_C Cuts at: 2421 2722 2781 3460 5537 6033 2421 Size: 301 59 679 2077 496 2722 Fragments arranged by size: 2722 2077 679 496 301 59 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 3251 1163 Size: 573 1515 4246 Fragments arranged by size: 4246 1515 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2523 3784 4545 5573 1540 Size: 275 186 522 1261 761 1028 2301 Fragments arranged by size: 2301 1261 1028 761 522 275 186 BsiHKAI G_wGCw'C Cuts at: 2121 2658 3218 4395 4480 5551 2121 Size: 537 560 1177 85 1071 2904 Fragments arranged by size: 2904 1177 1071 560 537 85 BsmI GAATG_Cn' Cuts at: 3845 3938 3845 Size: 93 6241 BsmBI CGTCTCn'nnnn_ Cuts at: 2672 2672 Size: 6334 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2035 2121 2256 2280 2327 2334 2422 Size: 399 245 86 135 24 47 7 88 Cuts at: 2422 2658 2741 2799 2908 3218 3254 3266 3317 Size: 236 83 58 109 310 36 12 51 Cuts at: 3317 3626 3653 4395 4480 5551 1391 Size: 309 27 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 399 310 309 245 236 135 109 88 86 85 83 58 51 47 36 27 24 12 7 BspEI T'CCGG_A Cuts at: 5997 5997 Size: 6334 BspHI T'CATG_A Cuts at: 4223 5953 4223 Size: 1730 4604 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2244 3588 3689 844 Size: 1400 1344 101 3489 Fragments arranged by size: 3489 1400 1344 101 BsrBI CCG'CTC Cuts at: 1394 1823 3277 3408 4221 1394 Size: 429 1454 131 813 3507 Fragments arranged by size: 3507 1454 813 429 131 BsrDI GCAATG_nn' Cuts at: 65 1248 4829 5003 65 Size: 1183 3581 174 1396 Fragments arranged by size: 3581 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2146 2195 2805 4978 5165 1700 Size: 110 336 49 610 2173 187 2869 Fragments arranged by size: 2869 2173 610 336 187 110 49 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6334 BssHII G'CGCG_C Cuts at: 1925 2706 1925 Size: 781 5553 BssSI C'ACGA_G Cuts at: 2792 4394 5406 2792 Size: 1602 1012 3720 Fragments arranged by size: 3720 1602 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 2251 2692 3088 794 Size: 1457 441 396 4040 Fragments arranged by size: 4040 1457 441 396 BstBI TT'CG_AA Cuts at: 5138 5138 Size: 6334 BstEII G'GTnAC_C Cuts at: 1184 2065 3340 1184 Size: 881 1275 4178 Fragments arranged by size: 4178 1275 881 BstXI CCAn_nnnn'nTGG Cuts at: 2188 2188 Size: 6334 BstYI r'GATC_y Cuts at: 1972 2589 2643 3369 3664 4031 4416 4433 5885 Size: 617 54 726 295 367 385 17 1452 Cuts at: 5885 5971 5983 6329 1972 Size: 86 12 346 1977 Fragments arranged by size: 1977 1452 726 617 385 367 346 295 86 54 17 12 BtgI C'CryG_G Cuts at: 513 1065 2181 2503 3294 3562 513 Size: 552 1116 322 791 268 3285 Fragments arranged by size: 3285 1116 791 552 322 268 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 3157 3498 487 Size: 683 56 1931 341 3323 Fragments arranged by size: 3323 1931 683 341 56 BtsI GCAGTG_nn' Cuts at: 3850 4644 4664 3850 Size: 794 20 5520 Fragments arranged by size: 5520 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6334 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6299 DraI TTT'AAA Cuts at: 3638 3638 Size: 6334 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5341 809 Size: 507 4025 1802 Fragments arranged by size: 4025 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2193 2197 2298 Size: 54 1425 50 275 18 363 4 101 Cuts at: 2298 2520 3528 3781 4670 6299 8 Size: 222 1008 253 889 1629 43 Fragments arranged by size: 1629 1425 1008 889 363 275 253 222 101 54 50 43 18 4 EagI C'GGCC_G Cuts at: 1537 1812 2520 3781 1537 Size: 275 708 1261 4090 Fragments arranged by size: 4090 1261 708 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2819 2843 2894 3510 4264 1353 Size: 1466 24 51 616 754 3423 Fragments arranged by size: 3423 1466 754 616 51 24 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 3616 4921 5295 5441 387 Size: 1064 138 2027 1305 374 146 1280 Fragments arranged by size: 2027 1305 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2039 2132 2174 2261 2462 2606 2843 Size: 393 407 93 42 87 201 144 237 Cuts at: 2843 2966 3146 3161 3230 4397 4994 5781 6049 Size: 123 180 15 69 1167 597 787 268 Cuts at: 6049 1239 Size: 1524 Fragments arranged by size: 1524 1167 787 597 407 393 268 237 201 180 144 123 93 87 69 42 15 EcoNI CCTnn'n_nnAGG Cuts at: 2948 3542 2948 Size: 594 5740 EcoO109I rG'GnC_Cy Cuts at: 2133 2270 2437 2833 2854 2904 3281 2133 Size: 137 167 396 21 50 377 5186 Fragments arranged by size: 5186 396 377 167 137 50 21 EcoRI G'AATT_C Cuts at: 3643 4021 3643 Size: 378 5956 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2239 2239 Size: 6334 FseI GG_CCGG'CC Cuts at: 2199 2199 Size: 6334 FspI TGC'GCA Cuts at: 2577 2879 4840 2577 Size: 302 1961 4071 Fragments arranged by size: 4071 1961 302 FspAI rTGC'GCAy Cuts at: 2577 2577 Size: 6334 HaeII r_GCGC'y Cuts at: 1678 2723 2812 3243 3389 5481 1678 Size: 1045 89 431 146 2092 2531 Fragments arranged by size: 2531 2092 1045 431 146 89 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2588 2620 4975 5007 5049 Size: 32 135 32 1016 32 2355 32 42 Cuts at: 5049 5081 1373 Size: 32 2626 Fragments arranged by size: 2626 2355 1016 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3678 3924 669 Size: 988 281 1740 246 3079 Fragments arranged by size: 3079 1740 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6334 HpaI GTT'AAC Cuts at: 3924 3924 Size: 6334 KasI G'GCGC_C Cuts at: 2808 3385 2808 Size: 577 5757 KpnI G_GTAC'C Cuts at: 3659 3659 Size: 6334 MfeI C'AATT_G Cuts at: 3933 3933 Size: 6334 MluI A'CGCG_T Cuts at: 6210 6210 Size: 6334 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 5448 5632 1305 Size: 4143 184 2007 Fragments arranged by size: 4143 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 3530 10 Size: 54 1425 343 1698 2814 Fragments arranged by size: 2814 1698 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 3221 3257 4292 4651 4810 518 Size: 648 2055 36 1035 359 159 2042 Fragments arranged by size: 2055 2042 1035 648 359 159 36 MspA1I CmG'CkG Cuts at: 1606 2013 2110 2505 2689 2995 3034 3750 4427 Size: 407 97 395 184 306 39 716 677 Cuts at: 4427 5575 5820 1606 Size: 1148 245 2120 Fragments arranged by size: 2120 1148 716 677 407 395 306 245 184 97 39 NaeI GCC'GGC Cuts at: 2148 2197 2148 Size: 49 6285 NarI GG'CG_CC Cuts at: 2809 3386 2809 Size: 577 5757 NcoI C'CATG_G Cuts at: 513 1065 2181 3294 513 Size: 552 1116 1113 3553 Fragments arranged by size: 3553 1116 1113 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 6334 NgoMIV G'CCGG_C Cuts at: 2146 2195 2146 Size: 49 6285 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6334 NotI GC'GGCC_GC Cuts at: 3781 3781 Size: 6334 NspI r_CATG'y Cuts at: 1132 1229 3505 3692 5237 1132 Size: 97 2276 187 1545 2229 Fragments arranged by size: 2276 2229 1545 187 97 PasI CC'CwG_GG Cuts at: 1348 3053 1348 Size: 1705 4629 PciI A'CATG_T Cuts at: 5233 5233 Size: 6334 PflMI CCAn_nnn'nTGG Cuts at: 1108 3426 1108 Size: 2318 4016 PfoI T'CCnGG_A Cuts at: 2439 2439 Size: 6334 PmeI GTTT'AAAC Cuts at: 3638 3638 Size: 6334 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4403 4435 5916 5948 4403 Size: 32 1481 32 4789 Fragments arranged by size: 4789 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2437 2833 3281 2437 Size: 396 448 5490 Fragments arranged by size: 5490 448 396 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6334 PsiI TTA'TAA Cuts at: 3904 3904 Size: 6334 PspOMI G'GGCC_C Cuts at: 2904 3262 2904 Size: 358 5976 PstI C_TGCA'G Cuts at: 830 3686 830 Size: 2856 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6334 PvuII CAG'CTG Cuts at: 2013 2689 2995 3034 2013 Size: 676 306 39 5313 Fragments arranged by size: 5313 676 306 39 SacII CC_GC'GG Cuts at: 2506 2506 Size: 6334 SalI G'TCGA_C Cuts at: 1936 3676 1936 Size: 1740 4594 SbfI CC_TGCA'GG Cuts at: 3686 3686 Size: 6334 ScaI AGT'ACT Cuts at: 4582 6294 4582 Size: 1712 4622 SexAI A'CCwGG_T Cuts at: 2892 2892 Size: 6334 SfcI C'TryA_G Cuts at: 826 1045 3682 4817 5498 5689 826 Size: 219 2637 1135 681 191 1471 Fragments arranged by size: 2637 1471 1135 681 219 191 SfoI GGC'GCC Cuts at: 2810 3387 2810 Size: 577 5757 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6334 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6334 SmaI CCC'GGG Cuts at: 1872 2469 3661 1872 Size: 597 1192 4545 Fragments arranged by size: 4545 1192 597 SmlI C'TyrA_G Cuts at: 820 1017 1943 2078 4438 5339 5601 820 Size: 197 926 135 2360 901 262 1553 Fragments arranged by size: 2360 1553 926 901 262 197 135 SnaBI TAC'GTA Cuts at: 493 4125 493 Size: 3632 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6334 SphI G_CATG'C Cuts at: 1132 3505 3692 1132 Size: 2373 187 3774 Fragments arranged by size: 3774 2373 187 SspI AAT'ATT Cuts at: 5 52 4258 5 Size: 47 4206 2081 Fragments arranged by size: 4206 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6334 StyI C'CwwG_G Cuts at: 513 1065 2181 3294 3345 3414 3773 513 Size: 552 1116 1113 51 69 359 3074 Fragments arranged by size: 3074 1116 1113 552 359 69 51 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3269 3325 4361 4378 4531 4716 5161 Size: 1255 1689 56 1036 17 153 185 445 Cuts at: 5161 325 Size: 1498 Fragments arranged by size: 1689 1498 1255 1036 445 185 153 56 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2202 4580 6292 Size: 274 80 33 51 1443 225 2378 1712 Cuts at: 6292 96 Size: 138 Fragments arranged by size: 2378 1712 1443 274 225 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3608 3640 6294 6326 3608 Size: 32 2654 32 3616 Fragments arranged by size: 3616 2654 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6334 XbaI T'CTAG_A Cuts at: 3670 3670 Size: 6334 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2188 3427 3457 2188 Size: 1239 30 5065 Fragments arranged by size: 5065 1239 30 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6334 XmaI C'CCGG_G Cuts at: 1870 2467 3659 1870 Size: 597 1192 4545 Fragments arranged by size: 4545 1192 597 XmnI GAAnn'nnTTC Cuts at: 4463 4463 Size: 6334 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4142 276 Size: 53 83 186 936 2608 2468 Fragments arranged by size: 2608 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI FalI FseI FspI FspAI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SacII SalI SbfI ScaI SexAI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI AscI AvrII BaeI BbvCI BfrBI BsiWI BstZ17I Bsu36I DraIII EcoICRI EcoRV NruI NsiI PacI PmlI PspXI PsrI RsrII SacI SanDI SapI SfiI SrfI SwaI