(Circular) (Six-base) MAPSORT of: pFN21AB5493.seq Check: 981 from: 1 to: 7429 pFN21AB5493 7429 bp With 182 enzymes: SgfI * October 20, 2009 10:33 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2632 3539 2632 Size: 907 6522 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 5239 278 Size: 53 83 186 936 3703 2468 Fragments arranged by size: 3703 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 4772 1937 Size: 2835 4594 Acc65I G'GTAC_C Cuts at: 4208 4750 4208 Size: 542 6887 AclI AA'CG_TT Cuts at: 1563 5556 5929 1563 Size: 3993 373 3063 Fragments arranged by size: 3993 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2163 2259 2342 2814 3605 3845 5492 6876 Size: 531 96 83 472 791 240 1647 1384 Cuts at: 6876 7144 1632 Size: 268 1917 Fragments arranged by size: 1917 1647 1384 791 531 472 268 240 96 83 AfeI AGC'GCT Cuts at: 2226 2226 Size: 7429 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 7232 AflIII A'CryG_T Cuts at: 1203 6328 7305 1203 Size: 5125 977 1327 Fragments arranged by size: 5125 1327 977 AgeI A'CCGG_T Cuts at: 6260 6260 Size: 7429 AhdI GACnn_n'nnGTC Cuts at: 6158 6158 Size: 7429 AleI CACnn'nnGTG Cuts at: 1166 2564 3785 1166 Size: 1398 1221 4810 Fragments arranged by size: 4810 1398 1221 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2707 1184 Size: 1523 5906 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3107 3139 3948 3980 3107 Size: 32 809 32 6556 Fragments arranged by size: 6556 809 32 32 AlwNI CAG_nnn'CTG Cuts at: 2137 2521 3647 3812 3838 4306 4532 5172 6744 Size: 384 1126 165 26 468 226 640 1572 Cuts at: 6744 2137 Size: 2822 Fragments arranged by size: 2822 1572 1126 640 468 384 226 165 26 ApaLI G'TGCA_C Cuts at: 5486 6642 5486 Size: 1156 6273 ApoI r'AATT_y Cuts at: 1493 2294 4139 4738 4964 5116 7225 1493 Size: 801 1845 599 226 152 2109 1697 Fragments arranged by size: 2109 1845 1697 801 599 226 152 AseI AT'TA_AT Cuts at: 160 1032 5983 160 Size: 872 4951 1606 Fragments arranged by size: 4951 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 7429 AvaI C'yCGr_G Cuts at: 1870 1943 3006 4754 1870 Size: 73 1063 1748 4545 Fragments arranged by size: 4545 1748 1063 73 BamHI G'GATC_C Cuts at: 4759 5126 4759 Size: 367 7062 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2029 3034 4208 4325 4750 Size: 325 217 627 242 1005 1174 117 425 Cuts at: 4750 6205 618 Size: 1455 1842 Fragments arranged by size: 1842 1455 1174 1005 627 425 325 242 217 117 BanII G_rGCy'C Cuts at: 1391 2444 3030 3337 3654 4748 1391 Size: 1053 586 307 317 1094 4072 Fragments arranged by size: 4072 1094 1053 586 317 307 BbeI G_GCGC'C Cuts at: 2033 2033 Size: 7429 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2847 3891 4589 928 Size: 971 948 1044 698 3768 Fragments arranged by size: 3768 1044 971 948 698 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 5620 5654 1903 Size: 34 3683 34 3678 Fragments arranged by size: 3683 3678 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2197 3652 4120 5321 6531 2197 Size: 1455 468 1201 1210 3095 Fragments arranged by size: 3095 1455 1210 1201 468 BclI T'GATC_A Cuts at: 1548 1548 Size: 7429 BfrBI ATG'CAT Cuts at: 2678 2678 Size: 7429 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 3725 4226 4572 Size: 107 122 71 631 605 2053 501 346 Cuts at: 4572 6040 136 Size: 1468 1525 Fragments arranged by size: 2053 1525 1468 631 605 501 346 122 107 71 BglII A'GATC_T Cuts at: 7424 7424 Size: 7429 BlpI GC'TnA_GC Cuts at: 4846 4846 Size: 7429 Bme1580I G_kGCm'C Cuts at: 1790 2283 2761 4328 5490 6646 1790 Size: 493 478 1567 1162 1156 2573 Fragments arranged by size: 2573 1567 1162 1156 493 478 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 7429 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3698 4610 5160 Size: 416 53 83 399 24 2281 912 550 Cuts at: 5160 6118 442 Size: 958 1753 Fragments arranged by size: 2281 1753 958 912 550 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 7429 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2037 2192 2249 2675 2939 3764 6089 1239 Size: 798 155 57 426 264 825 2325 2579 Fragments arranged by size: 2579 2325 825 798 426 264 155 57 Bpu10I CC'TnA_GC Cuts at: 2505 2727 3001 4133 2505 Size: 222 274 1132 5801 Fragments arranged by size: 5801 1132 274 222 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2408 3638 5554 6419 6717 2408 Size: 1230 1916 865 298 3120 Fragments arranged by size: 3120 1916 1230 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 3698 6092 882 Size: 617 2199 2394 2219 Fragments arranged by size: 2394 2219 2199 617 BsaAI yAC'GTr Cuts at: 493 1204 2722 5220 493 Size: 711 1518 2498 2702 Fragments arranged by size: 2702 2498 1518 711 BsaBI GATnn'nnATC Cuts at: 4155 7423 4155 Size: 3268 4161 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2030 5236 5618 6253 Size: 53 83 186 936 497 3206 382 635 Cuts at: 6253 275 Size: 1451 Fragments arranged by size: 3206 1451 936 635 497 382 186 83 53 BsaWI w'CCGG_w Cuts at: 3369 4062 5862 6260 6534 6681 7092 3369 Size: 693 1800 398 274 147 411 3706 Fragments arranged by size: 3706 1800 693 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2366 2396 2402 2432 3107 Size: 30 165 30 963 30 6 30 675 Cuts at: 3107 3137 3290 3320 3737 3767 3851 3881 3948 Size: 30 153 30 417 30 84 30 67 Cuts at: 3948 3978 1178 Size: 30 4629 Fragments arranged by size: 4629 963 675 417 165 153 84 67 30 30 30 30 30 30 30 30 30 6 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2024 2954 3115 3298 4010 4523 4673 1186 Size: 838 930 161 183 712 513 150 3942 Fragments arranged by size: 3942 930 838 712 513 183 161 150 BseYI C'CCAG_C Cuts at: 2043 6632 7128 2043 Size: 4589 496 2344 Fragments arranged by size: 4589 2344 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2057 3252 3723 3851 4026 1163 Size: 573 321 1195 471 128 175 4566 Fragments arranged by size: 4566 1195 573 471 321 175 128 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 4357 4879 5640 6668 1540 Size: 275 186 2356 522 761 1028 2301 Fragments arranged by size: 2356 2301 1028 761 522 275 186 BsiHKAI G_wGCw'C Cuts at: 3337 3654 3949 5490 5575 6646 3337 Size: 317 295 1541 85 1071 4120 Fragments arranged by size: 4120 1541 1071 317 295 85 BsmI GAATG_Cn' Cuts at: 2888 3154 4494 4940 5033 2888 Size: 266 1340 446 93 5284 Fragments arranged by size: 5284 1340 446 266 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2283 2444 2761 3030 3337 3654 3949 Size: 399 493 161 317 269 307 317 295 Cuts at: 3949 4328 4748 5490 5575 6646 1391 Size: 379 420 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 493 420 399 379 317 317 307 295 269 161 85 BspEI T'CCGG_A Cuts at: 3369 4062 7092 3369 Size: 693 3030 3706 Fragments arranged by size: 3706 3030 693 BspHI T'CATG_A Cuts at: 2470 4147 5318 7048 2470 Size: 1677 1171 1730 2851 Fragments arranged by size: 2851 1730 1677 1171 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2631 2632 3539 4784 844 Size: 1787 1 907 1245 3489 Fragments arranged by size: 3489 1787 1245 907 1 BsrBI CCG'CTC Cuts at: 1394 1823 2091 2101 5316 1394 Size: 429 268 10 3215 3507 Fragments arranged by size: 3507 3215 429 268 10 BsrDI GCAATG_nn' Cuts at: 65 1248 3601 4239 4520 5924 6098 65 Size: 1183 2353 638 281 1404 174 1396 Fragments arranged by size: 2353 1404 1396 1183 638 281 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2032 2901 4356 6073 6260 1700 Size: 110 222 869 1455 1717 187 2869 Fragments arranged by size: 2869 1717 1455 869 222 187 110 BsrGI T'GTAC_A Cuts at: 96 2974 96 Size: 2878 4551 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 7429 BssSI C'ACGA_G Cuts at: 2180 5489 6501 2180 Size: 3309 1012 3108 Fragments arranged by size: 3309 3108 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 3838 794 Size: 3044 4385 BstBI TT'CG_AA Cuts at: 4423 6233 4423 Size: 1810 5619 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 7429 BstXI CCAn_nnnn'nTGG Cuts at: 3784 4195 3784 Size: 411 7018 BstYI r'GATC_y Cuts at: 1972 4759 5126 5511 5528 6980 7066 7078 7424 Size: 2787 367 385 17 1452 86 12 346 Cuts at: 7424 1972 Size: 1977 Fragments arranged by size: 2787 1977 1452 385 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 3217 4188 4380 4630 513 Size: 552 939 1213 971 192 250 3312 Fragments arranged by size: 3312 1213 971 939 552 250 192 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 6690 Fragments arranged by size: 6690 683 56 BtsI GCAGTG_nn' Cuts at: 3091 3395 3500 4210 4557 4945 5739 5759 3091 Size: 304 105 710 347 388 794 20 4761 Fragments arranged by size: 4761 794 710 388 347 304 105 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 7429 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 7394 DraI TTT'AAA Cuts at: 4733 4733 Size: 7429 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 6436 809 Size: 507 5120 1802 Fragments arranged by size: 5120 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2391 3160 4030 Size: 54 1425 50 275 18 561 769 870 Cuts at: 4030 4377 4517 4650 4876 5765 7394 8 Size: 347 140 133 226 889 1629 43 Fragments arranged by size: 1629 1425 889 870 769 561 347 275 226 140 133 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 4876 1537 Size: 275 3064 4090 Fragments arranged by size: 4090 3064 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2139 3342 4286 5359 1353 Size: 786 1203 944 1073 3423 Fragments arranged by size: 3423 1203 1073 944 786 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 6016 6390 6536 387 Size: 1064 138 4427 374 146 1280 Fragments arranged by size: 4427 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 3335 3652 3335 Size: 317 7112 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2037 2163 2192 2249 2259 2342 2675 Size: 393 405 126 29 57 10 83 333 Cuts at: 2675 2814 2939 3605 3764 3845 5492 6089 6876 Size: 139 125 666 159 81 1647 597 787 Cuts at: 6876 7144 1239 Size: 268 1524 Fragments arranged by size: 1647 1524 787 666 597 405 393 333 268 159 139 126 125 83 81 57 29 10 EcoNI CCTnn'n_nnAGG Cuts at: 3707 3707 Size: 7429 EcoO109I rG'GnC_Cy Cuts at: 2950 3588 4267 4303 2950 Size: 638 679 36 6076 Fragments arranged by size: 6076 679 638 36 EcoRI G'AATT_C Cuts at: 4738 5116 4738 Size: 378 7051 FspI TGC'GCA Cuts at: 5935 5935 Size: 7429 HaeII r_GCGC'y Cuts at: 1678 2033 2228 6576 1678 Size: 355 195 4348 2531 Fragments arranged by size: 4348 2531 355 195 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2366 2398 2951 2983 3515 Size: 32 135 32 794 32 553 32 532 Cuts at: 3515 3547 3678 3710 3737 3769 4104 4136 6070 Size: 32 131 32 27 32 335 32 1934 Cuts at: 6070 6102 6144 6176 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 1934 794 553 532 335 135 131 42 32 32 32 32 32 32 32 32 32 32 27 HincII GTy'rAC Cuts at: 669 1657 1938 4773 5019 669 Size: 988 281 2835 246 3079 Fragments arranged by size: 3079 2835 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 7429 HpaI GTT'AAC Cuts at: 5019 5019 Size: 7429 KasI G'GCGC_C Cuts at: 2029 2029 Size: 7429 KpnI G_GTAC'C Cuts at: 4212 4754 4212 Size: 542 6887 MfeI C'AATT_G Cuts at: 5028 5028 Size: 7429 MluI A'CGCG_T Cuts at: 7305 7305 Size: 7429 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 6543 6727 1305 Size: 5238 184 2007 Fragments arranged by size: 5238 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2393 3162 4032 4652 10 Size: 54 1425 343 561 769 870 620 2787 Fragments arranged by size: 2787 1425 870 769 620 561 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2399 2475 2564 3542 3785 4193 5387 Size: 648 1233 76 89 978 243 408 1194 Cuts at: 5387 5746 5905 518 Size: 359 159 2042 Fragments arranged by size: 2042 1233 1194 978 648 408 359 243 159 89 76 MspA1I CmG'CkG Cuts at: 1606 2057 2545 3236 3838 4845 5522 6670 6915 Size: 451 488 691 602 1007 677 1148 245 Cuts at: 6915 1606 Size: 2120 Fragments arranged by size: 2120 1148 1007 691 677 602 488 451 245 NaeI GCC'GGC Cuts at: 2903 2903 Size: 7429 NarI GG'CG_CC Cuts at: 2030 2030 Size: 7429 NcoI C'CATG_G Cuts at: 513 1065 2004 4188 513 Size: 552 939 2184 3754 Fragments arranged by size: 3754 2184 939 552 NdeI CA'TA_TG Cuts at: 387 2680 3802 387 Size: 2293 1122 4014 Fragments arranged by size: 4014 2293 1122 NgoMIV G'CCGG_C Cuts at: 2901 2901 Size: 7429 NheI G'CTAG_C Cuts at: 1052 1052 Size: 7429 NotI GC'GGCC_GC Cuts at: 4876 4876 Size: 7429 NsiI A_TGCA'T Cuts at: 2680 2680 Size: 7429 NspI r_CATG'y Cuts at: 1132 1229 2653 4787 6332 1132 Size: 97 1424 2134 1545 2229 Fragments arranged by size: 2229 2134 1545 1424 97 PasI CC'CwG_GG Cuts at: 1348 2348 2761 1348 Size: 1000 413 6016 Fragments arranged by size: 6016 1000 413 PciI A'CATG_T Cuts at: 6328 6328 Size: 7429 PflMI CCAn_nnn'nTGG Cuts at: 1108 2312 4552 1108 Size: 1204 2240 3985 Fragments arranged by size: 3985 2240 1204 PfoI T'CCnGG_A Cuts at: 2916 4122 4413 2916 Size: 1206 291 5932 Fragments arranged by size: 5932 1206 291 PmeI GTTT'AAAC Cuts at: 4733 4733 Size: 7429 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3107 3139 3427 3459 3948 3980 5498 5530 7011 Size: 32 288 32 489 32 1518 32 1481 Cuts at: 7011 7043 3107 Size: 32 3493 Fragments arranged by size: 3493 1518 1481 489 288 32 32 32 32 32 PpuMI rG'GwC_Cy Cuts at: 2950 3588 4267 2950 Size: 638 679 6112 Fragments arranged by size: 6112 679 638 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 7429 PsiI TTA'TAA Cuts at: 4999 4999 Size: 7429 PstI C_TGCA'G Cuts at: 830 4781 830 Size: 3951 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 7429 PvuII CAG'CTG Cuts at: 3236 3838 3236 Size: 602 6827 SacI G_AGCT'C Cuts at: 3337 3654 3337 Size: 317 7112 SalI G'TCGA_C Cuts at: 1936 4771 1936 Size: 2835 4594 SapI GCTCTTCn'nnn_ Cuts at: 3342 3342 Size: 7429 SbfI CC_TGCA'GG Cuts at: 4781 4781 Size: 7429 ScaI AGT'ACT Cuts at: 5677 7389 5677 Size: 1712 5717 SfcI C'TryA_G Cuts at: 826 1045 4777 5912 6593 6784 826 Size: 219 3732 1135 681 191 1471 Fragments arranged by size: 3732 1471 1135 681 219 191 SfoI GGC'GCC Cuts at: 2031 2031 Size: 7429 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 7429 SgrAI Cr'CCGG_yG Cuts at: 1700 2032 1700 Size: 332 7097 SmaI CCC'GGG Cuts at: 1872 4756 1872 Size: 2884 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2423 3653 5533 6434 6696 820 Size: 197 926 480 1230 1880 901 262 1553 Fragments arranged by size: 1880 1553 1230 926 901 480 262 197 SnaBI TAC'GTA Cuts at: 493 5220 493 Size: 4727 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 7429 SphI G_CATG'C Cuts at: 1132 4787 1132 Size: 3655 3774 SspI AAT'ATT Cuts at: 5 52 5353 5 Size: 47 5301 2081 Fragments arranged by size: 5301 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 7429 StyI C'CwwG_G Cuts at: 513 1065 2004 3030 4188 4263 4868 513 Size: 552 939 1026 1158 75 605 3074 Fragments arranged by size: 3074 1158 1026 939 605 552 75 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2753 3299 5456 5473 5626 5811 6256 Size: 1255 1173 546 2157 17 153 185 445 Cuts at: 6256 325 Size: 1498 Fragments arranged by size: 2157 1498 1255 1173 546 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2872 2974 3303 Size: 274 80 33 51 1443 895 102 329 Cuts at: 3303 5675 7387 96 Size: 2372 1712 138 Fragments arranged by size: 2372 1712 1443 895 329 274 138 102 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2402 2434 3364 3396 3851 3883 7389 7421 2402 Size: 32 930 32 455 32 3506 32 2410 Fragments arranged by size: 3506 2410 930 455 32 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 3690 1316 Size: 2374 5055 XbaI T'CTAG_A Cuts at: 4765 4765 Size: 7429 XcmI CCAnnnn_n'nnnnTGG Cuts at: 3541 3784 4186 4195 3541 Size: 243 402 9 6775 Fragments arranged by size: 6775 402 243 9 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 7429 XmaI C'CCGG_G Cuts at: 1870 4754 1870 Size: 2884 4545 XmnI GAAnn'nnTTC Cuts at: 5558 5558 Size: 7429 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 5237 276 Size: 53 83 186 936 3703 2468 Fragments arranged by size: 3703 2468 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NsiI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PstI PvuI PvuII SacI SalI SapI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: ApaI AscI AvrII BaeI BbvCI BplI BsiWI BsmBI BstZ17I Bsu36I DraIII EcoRV FalI FseI FspAI NruI PacI PmlI PspOMI PspXI PsrI RsrII SacII SanDI SexAI SfiI SrfI SwaI