(Circular) (Six-base) MAPSORT of: pFN21AB5485.seq Check: 1476 from: 1 to: 6256 pFN21AB5485 6256 bp With 182 enzymes: SgfI * February 27, 2009 14:34 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 3076 3076 Size: 6256 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4066 278 Size: 53 83 186 936 2530 2468 Fragments arranged by size: 2530 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3599 1937 Size: 1662 4594 Acc65I G'GTAC_C Cuts at: 3577 3577 Size: 6256 AclI AA'CG_TT Cuts at: 1563 4383 4756 1563 Size: 2820 373 3063 Fragments arranged by size: 3063 2820 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2232 2491 3299 3534 4319 5703 5971 1632 Size: 600 259 808 235 785 1384 268 1917 Fragments arranged by size: 1917 1384 808 785 600 268 259 235 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6059 AflIII A'CryG_T Cuts at: 1203 5155 6132 1203 Size: 3952 977 1327 Fragments arranged by size: 3952 1327 977 AgeI A'CCGG_T Cuts at: 5087 5087 Size: 6256 AhdI GACnn_n'nnGTC Cuts at: 4985 4985 Size: 6256 AleI CACnn'nnGTG Cuts at: 1166 3515 1166 Size: 2349 3907 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6256 AlwNI CAG_nnn'CTG Cuts at: 3277 3999 5571 3277 Size: 722 1572 3962 Fragments arranged by size: 3962 1572 722 ApaI G_GGCC'C Cuts at: 2034 2597 2034 Size: 563 5693 ApaLI G'TGCA_C Cuts at: 4313 5469 4313 Size: 1156 5100 ApoI r'AATT_y Cuts at: 1493 2560 3565 3791 3943 6052 1493 Size: 1067 1005 226 152 2109 1697 Fragments arranged by size: 2109 1697 1067 1005 226 152 AseI AT'TA_AT Cuts at: 160 1032 4810 160 Size: 872 3778 1606 Fragments arranged by size: 3778 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6256 AvaI C'yCGr_G Cuts at: 1870 1943 2099 2253 3027 3039 3581 1870 Size: 73 156 154 774 12 542 4545 Fragments arranged by size: 4545 774 542 156 154 73 12 BamHI G'GATC_C Cuts at: 3586 3953 3586 Size: 367 5889 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2283 3577 5032 618 Size: 325 217 627 496 1294 1455 1842 Fragments arranged by size: 1842 1455 1294 627 496 325 217 BanII G_rGCy'C Cuts at: 1391 2034 2276 2597 2876 3439 3575 1391 Size: 643 242 321 279 563 136 4072 Fragments arranged by size: 4072 643 563 321 279 242 136 BbeI G_GCGC'C Cuts at: 2287 2287 Size: 6256 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2098 3340 928 Size: 971 199 1242 3844 Fragments arranged by size: 3844 1242 971 199 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3220 3254 4447 4481 1903 Size: 34 1283 34 1193 34 3678 Fragments arranged by size: 3678 1283 1193 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4148 5358 4148 Size: 1210 5046 BclI T'GATC_A Cuts at: 1548 2676 1548 Size: 1128 5128 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2072 2255 2459 Size: 107 122 71 631 605 400 183 204 Cuts at: 2459 3395 4867 136 Size: 936 1472 1525 Fragments arranged by size: 1525 1472 936 631 605 400 204 183 122 107 71 BglII A'GATC_T Cuts at: 3252 6251 3252 Size: 2999 3257 BlpI GC'TnA_GC Cuts at: 2637 3673 2637 Size: 1036 5220 Bme1580I G_kGCm'C Cuts at: 1790 2034 2597 3404 4317 5473 1790 Size: 244 563 807 913 1156 2573 Fragments arranged by size: 2573 1156 913 807 563 244 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6256 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3433 3987 4945 Size: 416 53 83 399 24 2016 554 958 Cuts at: 4945 442 Size: 1753 Fragments arranged by size: 2016 1753 958 554 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6256 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2208 3377 4916 1239 Size: 969 1169 1539 2579 Fragments arranged by size: 2579 1539 1169 969 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2825 4381 5246 5544 2825 Size: 1556 865 298 3537 Fragments arranged by size: 3537 1556 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2366 4919 882 Size: 617 867 2553 2219 Fragments arranged by size: 2553 2219 867 617 BsaAI yAC'GTr Cuts at: 493 1204 3327 4047 493 Size: 711 2123 720 2702 Fragments arranged by size: 2702 2123 720 711 BsaBI GATnn'nnATC Cuts at: 3272 6250 3272 Size: 2978 3278 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2284 4063 4445 5080 Size: 53 83 186 936 751 1779 382 635 Cuts at: 5080 275 Size: 1451 Fragments arranged by size: 1779 1451 936 751 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2349 2442 4689 5087 5361 5508 5919 2349 Size: 93 2247 398 274 147 411 2686 Fragments arranged by size: 2686 2247 411 398 274 147 93 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 6031 Fragments arranged by size: 6031 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 3263 3311 1186 Size: 2077 48 4131 Fragments arranged by size: 4131 2077 48 BseYI C'CCAG_C Cuts at: 2269 2457 2590 2596 2738 2825 2860 5459 5955 Size: 188 133 6 142 87 35 2599 496 Cuts at: 5955 2269 Size: 2570 Fragments arranged by size: 2599 2570 496 188 142 133 87 35 6 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 3005 3242 1163 Size: 573 1269 237 4177 Fragments arranged by size: 4177 1269 573 237 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3706 4467 5495 1540 Size: 275 186 1705 761 1028 2301 Fragments arranged by size: 2301 1705 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 4317 4402 5473 4317 Size: 85 1071 5100 Fragments arranged by size: 5100 1071 85 BsiWI C'GTAC_G Cuts at: 3327 3327 Size: 6256 BsmI GAATG_Cn' Cuts at: 2780 3767 3860 2780 Size: 987 93 5176 Fragments arranged by size: 5176 987 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2034 2276 2597 2876 3404 3439 3575 Size: 399 244 242 321 279 528 35 136 Cuts at: 3575 4317 4402 5473 1391 Size: 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 528 399 321 279 244 242 136 85 35 BspEI T'CCGG_A Cuts at: 5919 5919 Size: 6256 BspHI T'CATG_A Cuts at: 4145 5875 4145 Size: 1730 4526 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3076 3611 844 Size: 2232 535 3489 Fragments arranged by size: 3489 2232 535 BsrBI CCG'CTC Cuts at: 1394 1823 2344 2569 3016 4143 1394 Size: 429 521 225 447 1127 3507 Fragments arranged by size: 3507 1127 521 447 429 225 BsrDI GCAATG_nn' Cuts at: 65 1248 2748 3171 4751 4925 65 Size: 1183 1500 423 1580 174 1396 Fragments arranged by size: 1580 1500 1396 1183 423 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2310 2453 2966 4900 5087 1700 Size: 110 500 143 513 1934 187 2869 Fragments arranged by size: 2869 1934 513 500 187 143 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6256 BssHII G'CGCG_C Cuts at: 1925 2011 1925 Size: 86 6170 BssSI C'ACGA_G Cuts at: 4316 5328 4316 Size: 1012 5244 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6256 BstBI TT'CG_AA Cuts at: 5060 5060 Size: 6256 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6256 BstYI r'GATC_y Cuts at: 1972 3111 3252 3273 3586 3953 4338 4355 5807 Size: 1139 141 21 313 367 385 17 1452 Cuts at: 5807 5893 5905 6251 1972 Size: 86 12 346 1977 Fragments arranged by size: 1977 1452 1139 385 367 346 313 141 86 21 17 12 BtgI C'CryG_G Cuts at: 513 1065 2473 2555 2733 2913 3184 513 Size: 552 1408 82 178 180 271 3585 Fragments arranged by size: 3585 1408 552 271 180 178 82 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 3223 487 Size: 683 56 1997 3520 Fragments arranged by size: 3520 1997 683 56 BtsI GCAGTG_nn' Cuts at: 2247 3772 4566 4586 2247 Size: 1525 794 20 3917 Fragments arranged by size: 3917 1525 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6256 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 2782 2817 3193 3228 529 Size: 35 2218 35 376 35 3557 Fragments arranged by size: 3557 2218 376 35 35 35 DraI TTT'AAA Cuts at: 3560 3560 Size: 6256 DraIII CAC_nnn'GTG Cuts at: 3408 3408 Size: 6256 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5263 809 Size: 507 3947 1802 Fragments arranged by size: 3947 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2328 2667 2803 Size: 54 1425 50 275 18 498 339 136 Cuts at: 2803 3336 3475 3703 4592 6221 8 Size: 533 139 228 889 1629 43 Fragments arranged by size: 1629 1425 889 533 498 339 275 228 139 136 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3703 1537 Size: 275 1891 4090 Fragments arranged by size: 4090 1891 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2864 4186 1353 Size: 1511 1322 3423 Fragments arranged by size: 3423 1511 1322 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4843 5217 5363 387 Size: 1064 138 3254 374 146 1280 Fragments arranged by size: 3254 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2208 2232 2491 3299 3377 3534 4319 Size: 393 576 24 259 808 78 157 785 Cuts at: 4319 4916 5703 5971 1239 Size: 597 787 268 1524 Fragments arranged by size: 1524 808 787 785 597 576 393 268 259 157 78 24 EcoNI CCTnn'n_nnAGG Cuts at: 2678 3383 2678 Size: 705 5551 EcoO109I rG'GnC_Cy Cuts at: 2031 3388 2031 Size: 1357 4899 EcoRI G'AATT_C Cuts at: 3565 3943 3565 Size: 378 5878 FspI TGC'GCA Cuts at: 4762 4762 Size: 6256 HaeII r_GCGC'y Cuts at: 1678 2287 5403 1678 Size: 609 3116 2531 Fragments arranged by size: 3116 2531 609 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2181 2213 4897 4929 4971 Size: 32 135 32 609 32 2684 32 42 Cuts at: 4971 5003 1373 Size: 32 2626 Fragments arranged by size: 2684 2626 609 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2710 3415 3600 3846 669 Size: 988 281 772 705 185 246 3079 Fragments arranged by size: 3079 988 772 705 281 246 185 HindIII A'AGCT_T Cuts at: 748 748 Size: 6256 HpaI GTT'AAC Cuts at: 3846 3846 Size: 6256 KasI G'GCGC_C Cuts at: 2283 2283 Size: 6256 KpnI G_GTAC'C Cuts at: 3581 3581 Size: 6256 MfeI C'AATT_G Cuts at: 3855 3855 Size: 6256 MluI A'CGCG_T Cuts at: 6132 6132 Size: 6256 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 5370 5554 1305 Size: 4065 184 2007 Fragments arranged by size: 4065 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2669 10 Size: 54 1425 343 837 3597 Fragments arranged by size: 3597 1425 837 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2763 2885 3515 4214 4573 4732 518 Size: 648 1597 122 630 699 359 159 2042 Fragments arranged by size: 2042 1597 699 648 630 359 159 122 MspA1I CmG'CkG Cuts at: 1606 2269 3344 3672 4349 5497 5742 1606 Size: 663 1075 328 677 1148 245 2120 Fragments arranged by size: 2120 1148 1075 677 663 328 245 NaeI GCC'GGC Cuts at: 2312 2455 2968 2312 Size: 143 513 5600 Fragments arranged by size: 5600 513 143 NarI GG'CG_CC Cuts at: 2284 2284 Size: 6256 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 5704 NdeI CA'TA_TG Cuts at: 387 387 Size: 6256 NgoMIV G'CCGG_C Cuts at: 2310 2453 2966 2310 Size: 143 513 5600 Fragments arranged by size: 5600 513 143 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6256 NotI GC'GGCC_GC Cuts at: 3703 3703 Size: 6256 NspI r_CATG'y Cuts at: 1132 1229 3614 5159 1132 Size: 97 2385 1545 2229 Fragments arranged by size: 2385 2229 1545 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6256 PciI A'CATG_T Cuts at: 5155 5155 Size: 6256 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 6256 PfoI T'CCnGG_A Cuts at: 2972 2972 Size: 6256 PmeI GTTT'AAAC Cuts at: 3560 3560 Size: 6256 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4325 4357 5838 5870 4325 Size: 32 1481 32 4711 Fragments arranged by size: 4711 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6256 PsiI TTA'TAA Cuts at: 3826 3826 Size: 6256 PspOMI G'GGCC_C Cuts at: 2030 2593 2030 Size: 563 5693 PstI C_TGCA'G Cuts at: 830 3504 3608 830 Size: 2674 104 3478 Fragments arranged by size: 3478 2674 104 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6256 PvuII CAG'CTG Cuts at: 2269 3344 2269 Size: 1075 5181 SalI G'TCGA_C Cuts at: 1936 3598 1936 Size: 1662 4594 SapI GCTCTTCn'nnn_ Cuts at: 2864 2864 Size: 6256 SbfI CC_TGCA'GG Cuts at: 3608 3608 Size: 6256 ScaI AGT'ACT Cuts at: 4504 6216 4504 Size: 1712 4544 SexAI A'CCwGG_T Cuts at: 2409 2409 Size: 6256 SfcI C'TryA_G Cuts at: 826 1045 3500 3604 4739 5420 5611 826 Size: 219 2455 104 1135 681 191 1471 Fragments arranged by size: 2455 1471 1135 681 219 191 104 SfiI GGCCn_nnn'nGGCC Cuts at: 2255 2255 Size: 6256 SfoI GGC'GCC Cuts at: 2285 2285 Size: 6256 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6256 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6256 SmaI CCC'GGG Cuts at: 1872 2255 3029 3583 1872 Size: 383 774 554 4545 Fragments arranged by size: 4545 774 554 383 SmlI C'TyrA_G Cuts at: 820 1017 1943 2840 4360 5261 5523 820 Size: 197 926 897 1520 901 262 1553 Fragments arranged by size: 1553 1520 926 901 897 262 197 SnaBI TAC'GTA Cuts at: 493 4047 493 Size: 3554 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6256 SphI G_CATG'C Cuts at: 1132 3614 1132 Size: 2482 3774 SrfI GCCC'GGGC Cuts at: 2255 3029 2255 Size: 774 5482 SspI AAT'ATT Cuts at: 5 52 4180 5 Size: 47 4128 2081 Fragments arranged by size: 4128 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6256 StyI C'CwwG_G Cuts at: 513 1065 2279 2367 2601 2853 3243 3695 513 Size: 552 1214 88 234 252 390 452 3074 Fragments arranged by size: 3074 1214 552 452 390 252 234 88 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2084 3086 4283 4300 4453 4638 5083 Size: 1255 504 1002 1197 17 153 185 445 Cuts at: 5083 325 Size: 1498 Fragments arranged by size: 1498 1255 1197 1002 504 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 4502 6214 96 Size: 274 80 33 51 1443 2525 1712 138 Fragments arranged by size: 2525 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2161 2193 6216 6248 2161 Size: 32 4023 32 2169 Fragments arranged by size: 4023 2169 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 2426 1316 Size: 1110 5146 XbaI T'CTAG_A Cuts at: 3592 3592 Size: 6256 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2822 3040 3331 3446 2822 Size: 218 291 115 5632 Fragments arranged by size: 5632 291 218 115 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6256 XmaI C'CCGG_G Cuts at: 1870 2253 3027 3581 1870 Size: 383 774 554 4545 Fragments arranged by size: 4545 774 554 383 XmnI GAAnn'nnTTC Cuts at: 2162 4385 2162 Size: 2223 4033 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4064 276 Size: 53 83 186 936 2530 2468 Fragments arranged by size: 2530 2468 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsiWI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PshAI PsiI PspOMI PstI PvuI PvuII SalI SapI SbfI ScaI SexAI SfcI SfiI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SrfI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI AscI AvrII BaeI BbvCI BfrBI BplI Bpu10I BsmBI BstXI BstZ17I Bsu36I EcoICRI EcoRV FalI FseI FspAI NruI NsiI PacI PmlI PpuMI PspXI PsrI RsrII SacI SacII SanDI SwaI