Restriction map for
pFN21AB5485
(Linear) (Six-base) MAPSORT of: KIBB5485.seq Check: 7475 from: 1 to: 1551
KIBB5485.orf
With 182 enzymes: SgfI *
February 28, 2007 11:27 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 1071 1551
Size: 1071 480
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 227 486 1294 1529 1551
Size: 227 259 808 235 22
Fragments arranged by size:
808 259 235 227 22
AleI CACnn'nnGTG
Cuts at: 0 1510 1551
Size: 1510 41
AlwNI CAG_nnn'CTG
Cuts at: 0 1272 1551
Size: 1272 279
ApaI G_GGCC'C
Cuts at: 0 29 592 1551
Size: 29 563 959
Fragments arranged by size:
959 563 29
ApoI r'AATT_y
Cuts at: 0 555 1551
Size: 555 996
AvaI C'yCGr_G
Cuts at: 0 94 248 1022 1034 1551
Size: 94 154 774 12 517
Fragments arranged by size:
774 517 154 94 12
BanI G'GyrC_C
Cuts at: 0 278 1551
Size: 278 1273
BanII G_rGCy'C
Cuts at: 0 29 271 592 871 1434 1551
Size: 29 242 321 279 563 117
Fragments arranged by size:
563 321 279 242 117 29
BbeI G_GCGC'C
Cuts at: 0 282 1551
Size: 282 1269
BbsI GAAGACnn'nnnn_
Cuts at: 0 93 1335 1551
Size: 93 1242 216
Fragments arranged by size:
1242 216 93
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1215 1249 1551
Size: 1215 34 302
Fragments arranged by size:
1215 302 34
BclI T'GATC_A
Cuts at: 0 671 1551
Size: 671 880
BglI GCCn_nnn'nGGC
Cuts at: 0 67 250 454 1390 1551
Size: 67 183 204 936 161
Fragments arranged by size:
936 204 183 161 67
BglII A'GATC_T
Cuts at: 0 1247 1551
Size: 1247 304
BlpI GC'TnA_GC
Cuts at: 0 632 1551
Size: 632 919
Bme1580I G_kGCm'C
Cuts at: 0 29 592 1399 1551
Size: 29 563 807 152
Fragments arranged by size:
807 563 152 29
BmrI ACTGGGnnnn_n'
Cuts at: 0 1428 1551
Size: 1428 123
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 203 1372 1551
Size: 203 1169 179
Fragments arranged by size:
1169 203 179
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 820 1551
Size: 820 731
BsaI GGTCTCn'nnnn_
Cuts at: 0 361 1551
Size: 361 1190
BsaAI yAC'GTr
Cuts at: 0 1322 1551
Size: 1322 229
BsaBI GATnn'nnATC
Cuts at: 0 1267 1551
Size: 1267 284
BsaHI Gr'CG_yC
Cuts at: 0 279 1551
Size: 279 1272
BsaWI w'CCGG_w
Cuts at: 0 344 437 1551
Size: 344 93 1114
Fragments arranged by size:
1114 344 93
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 1258 1306 1551
Size: 1258 48 245
Fragments arranged by size:
1258 245 48
BseYI C'CCAG_C
Cuts at: 0 264 452 585 591 733 820 855 1551
Size: 264 188 133 6 142 87 35 696
Fragments arranged by size:
696 264 188 142 133 87 35 6
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1000 1237 1551
Size: 1000 237 314
Fragments arranged by size:
1000 314 237
BsiWI C'GTAC_G
Cuts at: 0 1322 1551
Size: 1322 229
BsmI GAATG_Cn'
Cuts at: 0 775 1551
Size: 775 776
Bsp1286I G_dGCh'C
Cuts at: 0 29 271 592 871 1399 1434 1551
Size: 29 242 321 279 528 35 117
Fragments arranged by size:
528 321 279 242 117 35 29
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 1071 1551
Size: 1071 480
BsrBI CCG'CTC
Cuts at: 0 339 564 1011 1551
Size: 339 225 447 540
Fragments arranged by size:
540 447 339 225
BsrDI GCAATG_nn'
Cuts at: 0 743 1166 1551
Size: 743 423 385
Fragments arranged by size:
743 423 385
BsrFI r'CCGG_y
Cuts at: 0 305 448 961 1551
Size: 305 143 513 590
Fragments arranged by size:
590 513 305 143
BssHII G'CGCG_C
Cuts at: 0 6 1551
Size: 6 1545
BstYI r'GATC_y
Cuts at: 0 1106 1247 1268 1551
Size: 1106 141 21 283
Fragments arranged by size:
1106 283 141 21
BtgI C'CryG_G
Cuts at: 0 468 550 728 908 1179 1551
Size: 468 82 178 180 271 372
Fragments arranged by size:
468 372 271 180 178 82
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 1218 1551
Size: 1218 333
BtsI GCAGTG_nn'
Cuts at: 0 242 1551
Size: 242 1309
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 777 812 1188 1223 1551
Size: 777 35 376 35 328
Fragments arranged by size:
777 376 328 35 35
DraIII CAC_nnn'GTG
Cuts at: 0 1403 1551
Size: 1403 148
EaeI y'GGCC_r
Cuts at: 0 323 662 798 1331 1470 1551
Size: 323 339 136 533 139 81
Fragments arranged by size:
533 339 323 139 136 81
EarI CTCTTCn'nnn_
Cuts at: 0 859 1551
Size: 859 692
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 203 227 486 1294 1372 1529 1551
Size: 203 24 259 808 78 157 22
Fragments arranged by size:
808 259 203 157 78 24 22
EcoNI CCTnn'n_nnAGG
Cuts at: 0 673 1378 1551
Size: 673 705 173
Fragments arranged by size:
705 673 173
EcoO109I rG'GnC_Cy
Cuts at: 0 26 1383 1551
Size: 26 1357 168
Fragments arranged by size:
1357 168 26
HaeII r_GCGC'y
Cuts at: 0 282 1551
Size: 282 1269
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 176 208 1551
Size: 176 32 1343
Fragments arranged by size:
1343 176 32
HincII GTy'rAC
Cuts at: 0 705 1410 1551
Size: 705 705 141
Fragments arranged by size:
705 705 141
KasI G'GCGC_C
Cuts at: 0 278 1551
Size: 278 1273
MscI TGG'CCA
Cuts at: 0 664 1551
Size: 664 887
MslI CAynn'nnrTG
Cuts at: 0 758 880 1510 1551
Size: 758 122 630 41
Fragments arranged by size:
758 630 122 41
MspA1I CmG'CkG
Cuts at: 0 264 1339 1551
Size: 264 1075 212
Fragments arranged by size:
1075 264 212
NaeI GCC'GGC
Cuts at: 0 307 450 963 1551
Size: 307 143 513 588
Fragments arranged by size:
588 513 307 143
NarI GG'CG_CC
Cuts at: 0 279 1551
Size: 279 1272
NgoMIV G'CCGG_C
Cuts at: 0 305 448 961 1551
Size: 305 143 513 590
Fragments arranged by size:
590 513 305 143
PfoI T'CCnGG_A
Cuts at: 0 967 1551
Size: 967 584
PspOMI G'GGCC_C
Cuts at: 0 25 588 1551
Size: 25 563 963
Fragments arranged by size:
963 563 25
PstI C_TGCA'G
Cuts at: 0 1499 1551
Size: 1499 52
PvuII CAG'CTG
Cuts at: 0 264 1339 1551
Size: 264 1075 212
Fragments arranged by size:
1075 264 212
SapI GCTCTTCn'nnn_
Cuts at: 0 859 1551
Size: 859 692
SexAI A'CCwGG_T
Cuts at: 0 404 1551
Size: 404 1147
SfcI C'TryA_G
Cuts at: 0 1495 1551
Size: 1495 56
SfiI GGCCn_nnn'nGGCC
Cuts at: 0 250 1551
Size: 250 1301
SfoI GGC'GCC
Cuts at: 0 280 1551
Size: 280 1271
SmaI CCC'GGG
Cuts at: 0 250 1024 1551
Size: 250 774 527
Fragments arranged by size:
774 527 250
SmlI C'TyrA_G
Cuts at: 0 835 1551
Size: 835 716
SrfI GCCC'GGGC
Cuts at: 0 250 1024 1551
Size: 250 774 527
Fragments arranged by size:
774 527 250
StyI C'CwwG_G
Cuts at: 0 274 362 596 848 1238 1551
Size: 274 88 234 252 390 313
Fragments arranged by size:
390 313 274 252 234 88
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 79 1081 1551
Size: 79 1002 470
Fragments arranged by size:
1002 470 79
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 156 188 1551
Size: 156 32 1363
Fragments arranged by size:
1363 156 32
Tth111I GACn'n_nGTC
Cuts at: 0 421 1551
Size: 421 1130
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 817 1035 1326 1441 1551
Size: 817 218 291 115 110
Fragments arranged by size:
817 291 218 115 110
XmaI C'CCGG_G
Cuts at: 0 248 1022 1551
Size: 248 774 529
Fragments arranged by size:
774 529 248
XmnI GAAnn'nnTTC
Cuts at: 0 157 1551
Size: 157 1394
Enzymes that do cut:
AarI AcuI AleI AlwNI ApaI ApoI AvaI BanI
BanII BbeI BbsI BcgI BclI BglI BglII BlpI
Bme1580I BmrI BpmI BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BseRI BseYI BsgI BsiWI BsmI Bsp1286I BspMI
BsrBI BsrDI BsrFI BssHII BstYI BtgI BtgZI BtsI
CspCI DraIII EaeI EarI Eco57MI EcoNI EcoO109I HaeII
Hin4I HincII KasI MscI MslI MspA1I NaeI NarI
NgoMIV PfoI PspOMI PstI PvuII SapI SexAI SfcI
SfiI SfoI SmaI SmlI SrfI StyI TaqII TstI
Tth111I XcmI XmaI XmnI
Enzymes that do not cut:
AatII AccI Acc65I AclI AfeI AflII AflIII AgeI
AhdI AlfI AloI ApaLI AscI AseI AsiSI AvrII
BaeI BamHI BbvCI BciVI BfrBI BmgBI BmtI BplI
Bpu10I BsaXI BsiEI BsiHKAI BsmBI BspEI BspHI BsrGI
BssSI BstAPI BstBI BstEII BstXI BstZ17I Bsu36I ClaI
DraI DrdI EagI EciI EcoICRI EcoRI EcoRV FalI
FseI FspI FspAI HindIII HpaI KpnI MfeI MluI
MmeI NcoI NdeI NheI NotI NruI NsiI NspI
PacI PasI PciI PflMI PmeI PmlI PpiI PpuMI
PshAI PsiI PspXI PsrI PvuI RsrII SacI SacII
SalI SanDI SbfI ScaI SgfI SgrAI SnaBI SpeI
SphI SspI StuI SwaI TatI XbaI XhoI ZraI