(Circular) (Six-base) MAPSORT of: pFN21AB5441.seq Check: 1935 from: 1 to: 5722 pFN21AB5441 5722 bp With 182 enzymes: SgfI * October 20, 2009 10:40 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3532 278 Size: 53 83 186 936 1996 2468 Fragments arranged by size: 2468 1996 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3065 1937 Size: 1128 4594 Acc65I G'GTAC_C Cuts at: 2183 3043 2183 Size: 860 4862 AclI AA'CG_TT Cuts at: 1563 3849 4222 1563 Size: 2286 373 3063 Fragments arranged by size: 3063 2286 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2258 2493 3785 5169 5437 1632 Size: 626 235 1292 1384 268 1917 Fragments arranged by size: 1917 1384 1292 626 268 235 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5525 AflIII A'CryG_T Cuts at: 1203 2809 4621 5598 1203 Size: 1606 1812 977 1327 Fragments arranged by size: 1812 1606 1327 977 AgeI A'CCGG_T Cuts at: 4553 4553 Size: 5722 AhdI GACnn_n'nnGTC Cuts at: 4451 4451 Size: 5722 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5722 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5722 AlwNI CAG_nnn'CTG Cuts at: 3465 5037 3465 Size: 1572 4150 ApaI G_GGCC'C Cuts at: 2202 2202 Size: 5722 ApaLI G'TGCA_C Cuts at: 3779 4935 3779 Size: 1156 4566 ApoI r'AATT_y Cuts at: 1493 2988 3031 3257 3409 5518 1493 Size: 1495 43 226 152 2109 1697 Fragments arranged by size: 2109 1697 1495 226 152 43 AseI AT'TA_AT Cuts at: 160 1032 4276 160 Size: 872 3244 1606 Fragments arranged by size: 3244 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5722 AvaI C'yCGr_G Cuts at: 1870 1943 3047 1870 Size: 73 1104 4545 Fragments arranged by size: 4545 1104 73 BamHI G'GATC_C Cuts at: 3052 3419 3052 Size: 367 5355 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2183 3043 4498 618 Size: 325 217 627 396 860 1455 1842 Fragments arranged by size: 1842 1455 860 627 396 325 217 BanII G_rGCy'C Cuts at: 1391 2202 2315 3041 1391 Size: 811 113 726 4072 Fragments arranged by size: 4072 811 726 113 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 4751 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3913 3947 1903 Size: 34 1976 34 3678 Fragments arranged by size: 3678 1976 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2675 3614 4824 2675 Size: 939 1210 3573 Fragments arranged by size: 3573 1210 939 BclI T'GATC_A Cuts at: 1548 1548 Size: 5722 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2339 4333 136 Size: 107 122 71 631 605 667 1994 1525 Fragments arranged by size: 1994 1525 667 631 605 122 107 71 BglII A'GATC_T Cuts at: 2631 2730 5717 2631 Size: 99 2987 2636 Fragments arranged by size: 2987 2636 99 BlpI GC'TnA_GC Cuts at: 3139 3139 Size: 5722 Bme1580I G_kGCm'C Cuts at: 1790 2139 2202 3783 4939 1790 Size: 349 63 1581 1156 2573 Fragments arranged by size: 2573 1581 1156 349 63 BmgBI CAC'GTC Cuts at: 1318 2389 2575 2812 1318 Size: 1071 186 237 4228 Fragments arranged by size: 4228 1071 237 186 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2923 3019 3453 Size: 416 53 83 399 24 1506 96 434 Cuts at: 3453 4411 442 Size: 958 1753 Fragments arranged by size: 1753 1506 958 434 416 399 96 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5722 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 4382 1239 Size: 3143 2579 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3847 4712 5010 3847 Size: 865 298 4559 Fragments arranged by size: 4559 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2298 4385 882 Size: 617 799 2087 2219 Fragments arranged by size: 2219 2087 799 617 BsaAI yAC'GTr Cuts at: 493 1204 2124 3513 493 Size: 711 920 1389 2702 Fragments arranged by size: 2702 1389 920 711 BsaBI GATnn'nnATC Cuts at: 2369 5716 2369 Size: 3347 2375 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3529 3911 4546 275 Size: 53 83 186 936 1996 382 635 1451 Fragments arranged by size: 1996 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2361 2816 4155 4553 4827 4974 5385 2361 Size: 455 1339 398 274 147 411 2698 Fragments arranged by size: 2698 1339 455 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2297 2327 2684 2714 1178 Size: 30 165 30 894 30 357 30 4186 Fragments arranged by size: 4186 894 357 165 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 1186 Size: 5722 BseYI C'CCAG_C Cuts at: 2328 2856 4925 5421 2328 Size: 528 2069 496 2629 Fragments arranged by size: 2629 2069 528 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2560 1163 Size: 573 824 4325 Fragments arranged by size: 4325 824 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3172 3933 4961 1540 Size: 275 186 1171 761 1028 2301 Fragments arranged by size: 2301 1171 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2571 3783 3868 4939 2571 Size: 1212 85 1071 3354 Fragments arranged by size: 3354 1212 1071 85 BsmI GAATG_Cn' Cuts at: 3233 3326 3233 Size: 93 5629 BsmBI CGTCTCn'nnnn_ Cuts at: 2704 2704 Size: 5722 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2139 2202 2315 2571 3041 3783 3868 Size: 399 349 63 113 256 470 742 85 Cuts at: 3868 4939 1391 Size: 1071 2174 Fragments arranged by size: 2174 1071 742 470 399 349 256 113 85 63 BspEI T'CCGG_A Cuts at: 5385 5385 Size: 5722 BspHI T'CATG_A Cuts at: 3611 5341 3611 Size: 1730 3992 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2424 3077 844 Size: 1580 653 3489 Fragments arranged by size: 3489 1580 653 BsrBI CCG'CTC Cuts at: 1394 1823 3609 1394 Size: 429 1786 3507 Fragments arranged by size: 3507 1786 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4217 4391 65 Size: 1183 2969 174 1396 Fragments arranged by size: 2969 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4366 4553 1700 Size: 110 2556 187 2869 Fragments arranged by size: 2869 2556 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5722 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5722 BssSI C'ACGA_G Cuts at: 3782 4794 3782 Size: 1012 4710 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5722 BstBI TT'CG_AA Cuts at: 4526 4526 Size: 5722 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5722 BstYI r'GATC_y Cuts at: 1972 2364 2631 2730 3052 3419 3804 3821 5273 Size: 392 267 99 322 367 385 17 1452 Cuts at: 5273 5359 5371 5717 1972 Size: 86 12 346 1977 Fragments arranged by size: 1977 1452 392 385 367 346 322 267 99 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2748 513 Size: 552 1683 3487 Fragments arranged by size: 3487 1683 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 4983 Fragments arranged by size: 4983 683 56 BtsI GCAGTG_nn' Cuts at: 3238 4032 4052 3238 Size: 794 20 4908 Fragments arranged by size: 4908 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5722 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5687 DraI TTT'AAA Cuts at: 2935 3026 2935 Size: 91 5631 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4729 809 Size: 507 3413 1802 Fragments arranged by size: 3413 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2046 2273 2724 Size: 54 1425 50 275 18 216 227 451 Cuts at: 2724 2833 3169 4058 5687 8 Size: 109 336 889 1629 43 Fragments arranged by size: 1629 1425 889 451 336 275 227 216 109 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3169 1537 Size: 275 1357 4090 Fragments arranged by size: 4090 1357 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2165 3652 1353 Size: 812 1487 3423 Fragments arranged by size: 3423 1487 812 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2423 4309 4683 4829 387 Size: 1064 138 834 1886 374 146 1280 Fragments arranged by size: 1886 1280 1064 834 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2258 2493 3785 4382 5169 5437 1239 Size: 393 626 235 1292 597 787 268 1524 Fragments arranged by size: 1524 1292 787 626 597 393 268 235 EcoO109I rG'GnC_Cy Cuts at: 2064 2233 2413 2064 Size: 169 180 5373 Fragments arranged by size: 5373 180 169 EcoRI G'AATT_C Cuts at: 2988 3031 3409 2988 Size: 43 378 5301 Fragments arranged by size: 5301 378 43 FspI TGC'GCA Cuts at: 2385 4228 2385 Size: 1843 3879 FspAI rTGC'GCAy Cuts at: 2385 2385 Size: 5722 HaeII r_GCGC'y Cuts at: 1678 4869 1678 Size: 3191 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2297 2329 4363 4395 4437 Size: 32 135 32 725 32 2034 32 42 Cuts at: 4437 4469 1373 Size: 32 2626 Fragments arranged by size: 2626 2034 725 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2098 2107 2578 3066 3312 669 Size: 988 281 160 9 471 488 246 3079 Fragments arranged by size: 3079 988 488 471 281 246 160 9 HindIII A'AGCT_T Cuts at: 748 2251 2475 748 Size: 1503 224 3995 Fragments arranged by size: 3995 1503 224 HpaI GTT'AAC Cuts at: 3312 3312 Size: 5722 KpnI G_GTAC'C Cuts at: 2187 3047 2187 Size: 860 4862 MfeI C'AATT_G Cuts at: 3321 3321 Size: 5722 MluI A'CGCG_T Cuts at: 5598 5598 Size: 5722 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2064 4836 5020 1305 Size: 759 2772 184 2007 Fragments arranged by size: 2772 2007 759 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2048 2275 2835 10 Size: 54 1425 343 216 227 560 2897 Fragments arranged by size: 2897 1425 560 343 227 216 54 MslI CAynn'nnrTG Cuts at: 518 1166 2379 3680 4039 4198 518 Size: 648 1213 1301 359 159 2042 Fragments arranged by size: 2042 1301 1213 648 359 159 MspA1I CmG'CkG Cuts at: 1606 3138 3815 4963 5208 1606 Size: 1532 677 1148 245 2120 Fragments arranged by size: 2120 1532 1148 677 245 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 5170 NdeI CA'TA_TG Cuts at: 387 387 Size: 5722 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5722 NotI GC'GGCC_GC Cuts at: 3169 3169 Size: 5722 NspI r_CATG'y Cuts at: 1132 1229 2427 2440 3080 4625 1132 Size: 97 1198 13 640 1545 2229 Fragments arranged by size: 2229 1545 1198 640 97 13 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5722 PciI A'CATG_T Cuts at: 4621 4621 Size: 5722 PflMI CCAn_nnn'nTGG Cuts at: 1108 2901 1108 Size: 1793 3929 PfoI T'CCnGG_A Cuts at: 2900 2900 Size: 5722 PmeI GTTT'AAAC Cuts at: 3026 3026 Size: 5722 PmlI CAC'GTG Cuts at: 2124 2124 Size: 5722 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3791 3823 5304 5336 3791 Size: 32 1481 32 4177 Fragments arranged by size: 4177 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2233 2413 2233 Size: 180 5542 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5722 PsiI TTA'TAA Cuts at: 3292 3292 Size: 5722 PspOMI G'GGCC_C Cuts at: 2198 2198 Size: 5722 PstI C_TGCA'G Cuts at: 830 3074 830 Size: 2244 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5722 SalI G'TCGA_C Cuts at: 1936 3064 1936 Size: 1128 4594 SbfI CC_TGCA'GG Cuts at: 3074 3074 Size: 5722 ScaI AGT'ACT Cuts at: 3008 3970 5682 3008 Size: 962 1712 3048 Fragments arranged by size: 3048 1712 962 SfcI C'TryA_G Cuts at: 826 1045 2647 2692 3070 4205 4886 5077 826 Size: 219 1602 45 378 1135 681 191 1471 Fragments arranged by size: 1602 1471 1135 681 378 219 191 45 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5722 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5722 SmaI CCC'GGG Cuts at: 1872 3049 1872 Size: 1177 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 3826 4727 4989 820 Size: 197 926 1883 901 262 1553 Fragments arranged by size: 1883 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3513 493 Size: 3020 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5722 SphI G_CATG'C Cuts at: 1132 3080 1132 Size: 1948 3774 SspI AAT'ATT Cuts at: 5 52 3646 5 Size: 47 3594 2081 Fragments arranged by size: 3594 2081 47 StuI AGG'CCT Cuts at: 1517 2502 1517 Size: 985 4737 StyI C'CwwG_G Cuts at: 513 1065 3161 513 Size: 552 2096 3074 Fragments arranged by size: 3074 2096 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2183 2819 3749 3766 3919 4104 4549 Size: 1255 603 636 930 17 153 185 445 Cuts at: 4549 325 Size: 1498 Fragments arranged by size: 1498 1255 930 636 603 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3006 3968 5680 Size: 274 80 33 51 1443 1029 962 1712 Cuts at: 5680 96 Size: 138 Fragments arranged by size: 1712 1443 1029 962 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5682 5714 5682 Size: 32 5690 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5722 XbaI T'CTAG_A Cuts at: 3058 3058 Size: 5722 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2315 2848 2315 Size: 533 5189 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5722 XmaI C'CCGG_G Cuts at: 1870 3047 1870 Size: 1177 4545 XmnI GAAnn'nnTTC Cuts at: 3851 3851 Size: 5722 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3530 276 Size: 53 83 186 936 1996 2468 Fragments arranged by size: 2468 1996 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FspI FspAI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI AscI AvrII BaeI BbeI BbvCI BfrBI BplI Bpu10I BsiWI BstXI BstZ17I Bsu36I DraIII EcoICRI EcoNI EcoRV FalI FseI KasI NaeI NarI NgoMIV NruI NsiI PacI PspXI PsrI PvuII RsrII SacI SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI