Restriction map for
pFN21AB5346
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# NO CUT ENZYMES ( 104 enzymes) #
##################################
Acc65I AccI AflII AgeI AocI
Aor51HI ApaI AscI AseI AsnI
Asp700I Asp718I AvrII BamHI BanI
BbeI BbrPI BbvCI BciVI BclI
BfaI BfrI BglI BlnI BlpI
Bpu1102I BsaAI BsaBI BsiEI BsiWI
BspDI BspHI BsrBI BsrDI BsrFI
BssHII Bst1107I BstBI BstEII BstPI
BstXI BstZ17I Bsu36I CelII Cfr10I
ClaI CpoI DraIII DrdI EagI
EclXI Eco47III Eco52I Eco81I EcoO65I
EcoRI EcoRV FbaI FseI HincII
HindII HpaI KasI KpnI MaeI
MamI MfeI MluI MunI NaeI
NarI NcoI NdeI NgoMIV NheI
NotI NruI NspV PacI PinAI
PmaCI PmeI PmlI PshAI PshBI
PvuI PvuII RcaI RsrII SalI
SapI SbfI ScaI SfiI SfoI
SfuI SgrAI SnaBI SpeI Sse8387I
StuI SwaI XbaI XmnI
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# ONE CUT ENZYMES ( 55 enzymes) #
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AatII 1424 GACGTC GACGT^C
AccIII 215 TCCGGA T^CCGGA
AclI 1452 AACGTT AA^CGTT
AcsI 1435 AAATTT [GA]^AATT[CT]
AflIII 11 ACATGT A^C[GA][CT]GT
AhdI 7 GACAACATGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Alw44I 1229 GTGCAC G^TGCAC
ApaLI 1229 GTGCAC G^TGCAC
ApoI 1435 AAATTT [GA]^AATT[CT]
AspEI 7 GACAACATGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspI 1582 GACGTGGTC GAC[ACGT]^[ACGT][ACGT]GTC
AviII 321 TGCGCA TGC^GCA
BcgI 324 GCAGGTTCGTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BglII 1100 AGATCT A^GATCT
BsaI 1475 GGTCTC GGTCTC(1/5)
BsaWI 215 TCCGGA [AT]^CCGG[AT]
BseAI 215 TCCGGA T^CCGGA
BsgI 590 GTGCAG GTGCAG(16/14)
BsmBI 1784 CGTCTC CGTCTC(1/5)
BspEI 215 TCCGGA T^CCGGA
BspLU11I 11 ACATGT A^CATGT
BspMI 324 GCAGGT ACCTGC(4/8)
DraI 1665 TTTAAA TTT^AAA
DsaI 596 CCGCGG C^C[GA][CT]GG
Eam1105I 7 GACAACATGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EarI 396 GAAGAG CTCTTC(1/4)
EcoT22I 1282 ATGCAT ATGCA^T
FauI 628 CCCGC CCCGC(4/6)
FspI 321 TGCGCA TGC^GCA
HaeII 680 GGCGCT [GA]GCGC^[CT]
Ksp632I 396 GAAGAG CTCTTC(1/4)
KspI 596 CCGCGG CCGC^GG
MroI 215 TCCGGA T^CCGGA
MspA1I 596 CCGCGG C[AC]G^C[GT]G
NsiI 1282 ATGCAT ATGCA^T
PaeR7I 1096 CTCGAG C^TCGAG
PflFI 1582 GACGTGGTC GAC[ACGT]^[ACGT][ACGT]GTC
PflMI 648 CCACAACCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PleI 298 GAGTC GAGTC(4/5)
Psp1406I 1452 AACGTT AA^CGTT
SacII 596 CCGCGG CCGC^GG
SexAI 653 ACCTGGT A^CC[AT]GGT
SmaI 1777 CCCGGG CCC^GGG
SmlI 1096 CTCGAG C^T[CT][GA]AG
SphI 1284 GCATGC GCATG^C
SspI 1076 AATATT AAT^ATT
TaqI 1097 TCGA T^CGA
Tsp45I 222 GTCAC ^GT[GC]AC
Tth111I 1582 GACGTGGTC GAC[ACGT]^[ACGT][ACGT]GTC
TthHB8I 1097 TCGA T^CGA
Van91I 648 CCACAACCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XcmI 515 CCAAAGAGCCTCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoI 1096 CTCGAG C^TCGAG
XmaCI 1777 CCCGGG C^CCGGG
XmaI 1777 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 19 enzymes) #
###################################
AvaI 1096 CTCGAG C^[CT]CG[GA]G
1777 CCCGGG
AvaII 723 GGACC G^G[AT]CC
1120 GGACC
BalI 371 TGGCCA TGG^CCA
761 TGGCCA
BsmFI 1119 GGGAC GGGAC(10/14)
1327 GGGAC
BsmI 288 GAATGC GAATGC(1/-1)
1303 GAATGC
BsoBI 1096 CTCGAG C^[CT]CG[GA]G
1777 CCCGGG
BssSI 1754 CACGAG CACGAG(-5/-1)
1226 CTCGTG
BstAPI 309 GCAGAAACTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
384 GCAGAAACTGC
EcoNI 246 CCTGAGTGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
1606 CCTGACGGAGG
HindIII 52 AAGCTT A^AGCTT
107 AAGCTT
MaeIII 222 GTCAC ^GT[ACGT]AC
1387 GTTAC
MluNI 371 TGGCCA TGG^CCA
761 TGGCCA
MscI 371 TGGCCA TGG^CCA
761 TGGCCA
MseI 1024 TTAA T^TAA
1666 TTAA
NspI 11 ACATGT [GA]CATG^[CT]
1284 GCATGC
PpuMI 722 AGGACCT [GA]G^G[AT]CC[CT]
1119 GGGACCT
PstI 391 CTGCAG CTGCA^G
1445 CTGCAG
SacI 238 GAGCTC GAGCT^C
965 GAGCTC
Tru9I 1024 TTAA T^TAA
1666 TTAA
#########################################
# 3 OR MORE CUT ENZYMES ( 80 enzymes) #
#########################################
AccII AciI AcyI AfaI AluI
AlwI AlwNI AspHI BanII BbsI
BbvI BcnI BmyI BpmI BpuAI
BsaHI BsaJI BseRI BsiHKAI BsiYI
BslI BsmAI Bsp1286I Bsp1407I BsrGI
BsrI BssKI BstF5I BstNI BstUI
BstYI Cac8I CfoI Cfr13I DdeI
DpnII DraII EaeI EcoO109I EcoRII
EcoT14I Fnu4HI FokI HaeIII HapII
HgaI HhaI Hin1I HinP1I HinfI
HpaII HphI ItaI MaeII MboI
MboII MflI MnlI MslI MspI
MvaI MvnI MwoI NciI NdeII
NlaIII NlaIV RsaI Sau3AI Sau96I
ScrFI SfaNI SfcI SspBI StyI
TfiI TseI Tsp509I TspRI XhoII