Restriction map for
pFN21AB5346
(Circular) (Six-base) MAPSORT of: pFN21AB5346.seq Check: 8868 from: 1 to: 6559
pFN21AB5346 6559 bp
With 182 enzymes: SgfI *
February 27, 2009 14:34 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3433 4369 278
Size: 53 83 186 936 1897 936 2468
Fragments arranged by size:
2468 1897 936 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3902 1937
Size: 1965 4594
Acc65I G'GTAC_C
Cuts at: 3880 3880
Size: 6559
AclI AA'CG_TT
Cuts at: 1563 3458 3858 4686 5059 1563
Size: 1895 400 828 373 3063
Fragments arranged by size:
3063 1895 828 400 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2183 2727 3315 3764 4622 6006 6274 1632
Size: 551 544 588 449 858 1384 268 1917
Fragments arranged by size:
1917 1384 858 588 551 544 449 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6362
AflIII A'CryG_T
Cuts at: 1203 2016 5458 6435 1203
Size: 813 3442 977 1327
Fragments arranged by size:
3442 1327 977 813
AgeI A'CCGG_T
Cuts at: 5390 5390
Size: 6559
AhdI GACnn_n'nnGTC
Cuts at: 2017 5288 2017
Size: 3271 3288
AleI CACnn'nnGTG
Cuts at: 1166 2027 2164 2592 1166
Size: 861 137 428 5133
Fragments arranged by size:
5133 861 428 137
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2610 1184
Size: 1426 5133
AlwNI CAG_nnn'CTG
Cuts at: 2107 2320 2395 2722 4302 5874 2107
Size: 213 75 327 1580 1572 2792
Fragments arranged by size:
2792 1580 1572 327 213 75
ApaLI G'TGCA_C
Cuts at: 3234 4616 5772 3234
Size: 1382 1156 4021
Fragments arranged by size:
4021 1382 1156
ApoI r'AATT_y
Cuts at: 1493 3440 3868 4094 4246 6355 1493
Size: 1947 428 226 152 2109 1697
Fragments arranged by size:
2109 1947 1697 428 226 152
AseI AT'TA_AT
Cuts at: 160 1032 5113 160
Size: 872 4081 1606
Fragments arranged by size:
4081 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6559
AvaI C'yCGr_G
Cuts at: 1870 1943 3101 3782 3884 1870
Size: 73 1158 681 102 4545
Fragments arranged by size:
4545 1158 681 102 73
BamHI G'GATC_C
Cuts at: 3889 4256 3889
Size: 367 6192
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3880 5335 618
Size: 325 217 627 2093 1455 1842
Fragments arranged by size:
2093 1842 1455 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2247 2974 3100 3878 1391
Size: 856 727 126 778 4072
Fragments arranged by size:
4072 856 778 727 126
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2049 2562 2744 3849 928
Size: 971 150 513 182 1105 3638
Fragments arranged by size:
3638 1105 971 513 182 150
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2318 2352 4750 4784 1903
Size: 34 381 34 2398 34 3678
Fragments arranged by size:
3678 2398 381 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4451 5661 4451
Size: 1210 5349
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6559
BfrBI ATG'CAT
Cuts at: 3289 3289
Size: 6559
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 5170 136
Size: 107 122 71 631 605 3498 1525
Fragments arranged by size:
3498 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 3105 6554 3105
Size: 3449 3110
BlpI GC'TnA_GC
Cuts at: 3976 3976
Size: 6559
Bme1580I G_kGCm'C
Cuts at: 1790 2706 3238 3421 4620 5776 1790
Size: 916 532 183 1199 1156 2573
Fragments arranged by size:
2573 1199 1156 916 532 183
BmgBI CAC'GTC
Cuts at: 1318 2764 3589 1318
Size: 1446 825 4288
Fragments arranged by size:
4288 1446 825
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3131 4290 5248
Size: 416 53 83 399 24 1714 1159 958
Cuts at: 5248 442
Size: 1753
Fragments arranged by size:
1753 1714 1159 958 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6559
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2174 2252 2447 2468 2744 2753 5219 1239
Size: 935 78 195 21 276 9 2466 2579
Fragments arranged by size:
2579 2466 935 276 195 78 21 9
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4684 5549 5847 4684
Size: 865 298 5396
Fragments arranged by size:
5396 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 3486 5222 882
Size: 617 1987 1736 2219
Fragments arranged by size:
2219 1987 1736 617
BsaAI yAC'GTr
Cuts at: 493 1204 4350 493
Size: 711 3146 2702
Fragments arranged by size:
3146 2702 711
BsaBI GATnn'nnATC
Cuts at: 6553 6553
Size: 6559
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3430 3788 3846 4366
Size: 53 83 186 936 1897 358 58 520
Cuts at: 4366 4748 5383 275
Size: 382 635 1451
Fragments arranged by size:
1897 1451 936 635 520 382 358 186
83 58 53
BsaWI w'CCGG_w
Cuts at: 2220 4992 5390 5664 5811 6222 2220
Size: 2772 398 274 147 411 2557
Fragments arranged by size:
2772 2557 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2342 2372 1178
Size: 30 165 30 939 30 5365
Fragments arranged by size:
5365 939 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2216 2444 2447 1186
Size: 1030 228 3 5298
Fragments arranged by size:
5298 1030 228 3
BseYI C'CCAG_C
Cuts at: 2610 5762 6258 2610
Size: 3152 496 2911
Fragments arranged by size:
3152 2911 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2616 1163
Size: 573 880 5106
Fragments arranged by size:
5106 880 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 4009 4770 5798 1540
Size: 275 186 2008 761 1028 2301
Fragments arranged by size:
2301 2008 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2130 2247 2974 3238 3534 4620 4705 5776 2130
Size: 117 727 264 296 1086 85 1071 2913
Fragments arranged by size:
2913 1086 1071 727 296 264 117 85
BsmI GAATG_Cn'
Cuts at: 2299 3314 4070 4163 2299
Size: 1015 756 93 4695
Fragments arranged by size:
4695 1015 756 93
BsmBI CGTCTCn'nnnn_
Cuts at: 3795 3795
Size: 6559
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2130 2247 2706 2974 3100 3238 3421
Size: 399 340 117 459 268 126 138 183
Cuts at: 3421 3534 3878 4620 4705 5776 1391
Size: 113 344 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 459 399 344 340 268
183 138 126 117 113 85
BspEI T'CCGG_A
Cuts at: 2220 6222 2220
Size: 4002 2557
BspHI T'CATG_A
Cuts at: 4448 6178 4448
Size: 1730 4829
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2320 3914 844
Size: 1476 1594 3489
Fragments arranged by size:
3489 1594 1476
BsrBI CCG'CTC
Cuts at: 1394 1823 4446 1394
Size: 429 2623 3507
Fragments arranged by size:
3507 2623 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 5054 5228 65
Size: 1183 3806 174 1396
Fragments arranged by size:
3806 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 5203 5390 1700
Size: 110 3393 187 2869
Fragments arranged by size:
3393 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 2031 2796 3213 96
Size: 1935 765 417 3442
Fragments arranged by size:
3442 1935 765 417
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6559
BssSI C'ACGA_G
Cuts at: 3231 3759 4619 5631 3231
Size: 528 860 1012 4159
Fragments arranged by size:
4159 1012 860 528
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2320 2395 794
Size: 1526 75 4958
Fragments arranged by size:
4958 1526 75
BstBI TT'CG_AA
Cuts at: 5363 5363
Size: 6559
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6559
BstYI r'GATC_y
Cuts at: 1972 2628 3105 3665 3889 4256 4641 4658 6110
Size: 656 477 560 224 367 385 17 1452
Cuts at: 6110 6196 6208 6554 1972
Size: 86 12 346 1977
Fragments arranged by size:
1977 1452 656 560 477 385 367 346
224 86 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2601 513
Size: 552 1536 4471
Fragments arranged by size:
4471 1536 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5820
Fragments arranged by size:
5820 683 56
BtsI GCAGTG_nn'
Cuts at: 2599 3685 3727 3838 4075 4869 4889 2599
Size: 1086 42 111 237 794 20 4269
Fragments arranged by size:
4269 1086 794 237 111 42 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6559
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6524
DraI TTT'AAA
Cuts at: 3672 3863 3672
Size: 191 6368
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5566 809
Size: 507 4250 1802
Fragments arranged by size:
4250 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2376 2766 3795
Size: 54 1425 50 275 18 546 390 1029
Cuts at: 3795 4006 4895 6524 8
Size: 211 889 1629 43
Fragments arranged by size:
1629 1425 1029 889 546 390 275 211
54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 4006 1537
Size: 275 2194 4090
Fragments arranged by size:
4090 2194 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2396 4489 1353
Size: 1043 2093 3423
Fragments arranged by size:
3423 2093 1043
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 5146 5520 5666 387
Size: 1064 138 3557 374 146 1280
Fragments arranged by size:
3557 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2245 2972 2245
Size: 727 5832
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2174 2183 2252 2447 2468 2727 2744
Size: 393 542 9 69 195 21 259 17
Cuts at: 2744 2753 3315 3764 4622 5219 6006 6274 1239
Size: 9 562 449 858 597 787 268 1524
Fragments arranged by size:
1524 858 787 597 562 542 449 393
268 259 195 69 21 17 9 9
EcoNI CCTnn'n_nnAGG
Cuts at: 2255 3615 2255
Size: 1360 5199
EcoO109I rG'GnC_Cy
Cuts at: 2719 2728 3125 2719
Size: 9 397 6153
Fragments arranged by size:
6153 397 9
EcoRI G'AATT_C
Cuts at: 3868 4246 3868
Size: 378 6181
FspI TGC'GCA
Cuts at: 2328 5065 2328
Size: 2737 3822
HaeII r_GCGC'y
Cuts at: 1678 2689 5706 1678
Size: 1011 3017 2531
Fragments arranged by size:
3017 2531 1011
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2003 2035 2192 2224 3214
Size: 32 135 32 431 32 157 32 990
Cuts at: 3214 3246 3320 3352 5200 5232 5274 5306 1373
Size: 32 74 32 1848 32 42 32 2626
Fragments arranged by size:
2626 1848 990 431 157 135 74 42
32 32 32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3903 4149 669
Size: 988 281 1965 246 3079
Fragments arranged by size:
3079 1965 988 281 246
HindIII A'AGCT_T
Cuts at: 748 2057 2112 748
Size: 1309 55 5195
Fragments arranged by size:
5195 1309 55
HpaI GTT'AAC
Cuts at: 4149 4149
Size: 6559
KpnI G_GTAC'C
Cuts at: 3884 3884
Size: 6559
MfeI C'AATT_G
Cuts at: 4158 4158
Size: 6559
MluI A'CGCG_T
Cuts at: 6435 6435
Size: 6559
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2031 2220 3410 5673 5857 1305
Size: 726 189 1190 2263 184 2007
Fragments arranged by size:
2263 2007 1190 726 189 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2378 2768 10
Size: 54 1425 343 546 390 3801
Fragments arranged by size:
3801 1425 546 390 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2027 2164 2292 2592 3012 3284 4517
Size: 648 861 137 128 300 420 272 1233
Cuts at: 4517 4876 5035 518
Size: 359 159 2042
Fragments arranged by size:
2042 1233 861 648 420 359 300 272
159 137 128
MspA1I CmG'CkG
Cuts at: 1606 2603 3975 4652 5800 6045 1606
Size: 997 1372 677 1148 245 2120
Fragments arranged by size:
2120 1372 1148 997 677 245
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 6007
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6559
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6559
NotI GC'GGCC_GC
Cuts at: 4006 4006
Size: 6559
NsiI A_TGCA'T
Cuts at: 3291 3291
Size: 6559
NspI r_CATG'y
Cuts at: 1132 1229 2020 3293 3917 5462 1132
Size: 97 791 1273 624 1545 2229
Fragments arranged by size:
2229 1545 1273 791 624 97
PasI CC'CwG_GG
Cuts at: 1348 2564 1348
Size: 1216 5343
PciI A'CATG_T
Cuts at: 2016 5458 2016
Size: 3442 3117
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2659 1108
Size: 1551 5008
PmeI GTTT'AAAC
Cuts at: 3863 3863
Size: 6559
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4628 4660 6141 6173 4628
Size: 32 1481 32 5014
Fragments arranged by size:
5014 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2728 3125 2728
Size: 397 6162
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6559
PsiI TTA'TAA
Cuts at: 4129 4129
Size: 6559
PstI C_TGCA'G
Cuts at: 830 2400 3454 3911 830
Size: 1570 1054 457 3478
Fragments arranged by size:
3478 1570 1054 457
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6559
SacI G_AGCT'C
Cuts at: 2247 2974 2247
Size: 727 5832
SacII CC_GC'GG
Cuts at: 2604 2604
Size: 6559
SalI G'TCGA_C
Cuts at: 1936 3901 1936
Size: 1965 4594
SbfI CC_TGCA'GG
Cuts at: 3911 3911
Size: 6559
ScaI AGT'ACT
Cuts at: 4807 6519 4807
Size: 1712 4847
SexAI A'CCwGG_T
Cuts at: 2658 2658
Size: 6559
SfcI C'TryA_G
Cuts at: 826 1045 2396 3258 3450 3907 5042 5723 5914
Size: 219 1351 862 192 457 1135 681 191
Cuts at: 5914 826
Size: 1471
Fragments arranged by size:
1471 1351 1135 862 681 457 219 192
191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6559
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6559
SmaI CCC'GGG
Cuts at: 1872 3784 3886 1872
Size: 1912 102 4545
Fragments arranged by size:
4545 1912 102
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3101 4663 5564 5826 820
Size: 197 926 1158 1562 901 262 1553
Fragments arranged by size:
1562 1553 1158 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 4350 493
Size: 3857 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6559
SphI G_CATG'C
Cuts at: 1132 3293 3917 1132
Size: 2161 624 3774
Fragments arranged by size:
3774 2161 624
SspI AAT'ATT
Cuts at: 5 52 3083 4483 5
Size: 47 3031 1400 2081
Fragments arranged by size:
3031 2081 1400 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6559
StyI C'CwwG_G
Cuts at: 513 1065 2790 2847 3412 3998 513
Size: 552 1725 57 565 586 3074
Fragments arranged by size:
3074 1725 586 565 552 57
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 4586 4603 4756 4941 5386 325
Size: 1255 3006 17 153 185 445 1498
Fragments arranged by size:
3006 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2031 2796 2940
Size: 274 80 33 51 1443 54 765 144
Cuts at: 2940 3213 4805 6517 96
Size: 273 1592 1712 138
Fragments arranged by size:
1712 1592 1443 765 274 273 144 138
80 54 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2342 2374 6519 6551 2342
Size: 32 4145 32 2350
Fragments arranged by size:
4145 2350 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 3590 1316
Size: 2274 4285
XbaI T'CTAG_A
Cuts at: 3895 3895
Size: 6559
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2527 2527
Size: 6559
XhoI C'TCGA_G
Cuts at: 1943 3101 1943
Size: 1158 5401
XmaI C'CCGG_G
Cuts at: 1870 3782 3884 1870
Size: 1912 102 4545
Fragments arranged by size:
4545 1912 102
XmnI GAAnn'nnTTC
Cuts at: 4688 4688
Size: 6559
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3431 4367 276
Size: 53 83 186 936 1897 936 2468
Fragments arranged by size:
2468 1897 936 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbsI BcgI BciVI BclI
BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI
ClaI CspCI DraI DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I
HincII HindIII HpaI KpnI MfeI MluI MmeI MscI
MslI MspA1I NcoI NdeI NheI NotI NsiI NspI
PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI
PstI PvuI SacI SacII SalI SbfI ScaI SexAI
SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI
SspI StuI StyI TaqII TatI TstI Tth111I XbaI
XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbeI
BbvCI BplI Bpu10I BsiWI BstXI BstZ17I Bsu36I DraIII
EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV
NruI PacI PfoI PmlI PspOMI PspXI PsrI PvuII
RsrII SanDI SapI SfiI SfoI SrfI SwaI