Restriction map for
pFN21AB5346
(Linear) (Six-base) MAPSORT of: KIBB5346.seq Check: 3474 from: 1 to: 1854
KIBB5346.orf
With 182 enzymes: SgfI *
February 28, 2007 11:27 ..
AatII G_ACGT'C
Cuts at: 0 1428 1854
Size: 1428 426
AclI AA'CG_TT
Cuts at: 0 1453 1854
Size: 1453 401
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 178 722 1310 1759 1854
Size: 178 544 588 449 95
Fragments arranged by size:
588 544 449 178 95
AflIII A'CryG_T
Cuts at: 0 11 1854
Size: 11 1843
AhdI GACnn_n'nnGTC
Cuts at: 0 12 1854
Size: 12 1842
AleI CACnn'nnGTG
Cuts at: 0 22 159 587 1854
Size: 22 137 428 1267
Fragments arranged by size:
1267 428 137 22
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 605 1854
Size: 605 1249
AlwNI CAG_nnn'CTG
Cuts at: 0 102 315 390 717 1854
Size: 102 213 75 327 1137
Fragments arranged by size:
1137 327 213 102 75
ApaLI G'TGCA_C
Cuts at: 0 1229 1854
Size: 1229 625
ApoI r'AATT_y
Cuts at: 0 1435 1854
Size: 1435 419
AvaI C'yCGr_G
Cuts at: 0 1096 1777 1854
Size: 1096 681 77
Fragments arranged by size:
1096 681 77
BanII G_rGCy'C
Cuts at: 0 242 969 1095 1854
Size: 242 727 126 759
Fragments arranged by size:
759 727 242 126
BbsI GAAGACnn'nnnn_
Cuts at: 0 44 557 739 1844 1854
Size: 44 513 182 1105 10
Fragments arranged by size:
1105 513 182 44 10
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 313 347 1854
Size: 313 34 1507
Fragments arranged by size:
1507 313 34
BfrBI ATG'CAT
Cuts at: 0 1284 1854
Size: 1284 570
BglII A'GATC_T
Cuts at: 0 1100 1854
Size: 1100 754
Bme1580I G_kGCm'C
Cuts at: 0 701 1233 1416 1854
Size: 701 532 183 438
Fragments arranged by size:
701 532 438 183
BmgBI CAC'GTC
Cuts at: 0 759 1584 1854
Size: 759 825 270
Fragments arranged by size:
825 759 270
BmrI ACTGGGnnnn_n'
Cuts at: 0 1126 1854
Size: 1126 728
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 169 247 442 463 739 748 1854
Size: 169 78 195 21 276 9 1106
Fragments arranged by size:
1106 276 195 169 78 21 9
BsaI GGTCTCn'nnnn_
Cuts at: 0 1481 1854
Size: 1481 373
BsaHI Gr'CG_yC
Cuts at: 0 1425 1783 1841 1854
Size: 1425 358 58 13
Fragments arranged by size:
1425 358 58 13
BsaWI w'CCGG_w
Cuts at: 0 215 1854
Size: 215 1639
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 337 367 1854
Size: 337 30 1487
Fragments arranged by size:
1487 337 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 211 439 442 1854
Size: 211 228 3 1412
Fragments arranged by size:
1412 228 211 3
BseYI C'CCAG_C
Cuts at: 0 605 1854
Size: 605 1249
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 611 1854
Size: 611 1243
BsiHKAI G_wGCw'C
Cuts at: 0 125 242 969 1233 1529 1854
Size: 125 117 727 264 296 325
Fragments arranged by size:
727 325 296 264 125 117
BsmI GAATG_Cn'
Cuts at: 0 294 1309 1854
Size: 294 1015 545
Fragments arranged by size:
1015 545 294
BsmBI CGTCTCn'nnnn_
Cuts at: 0 1790 1854
Size: 1790 64
Bsp1286I G_dGCh'C
Cuts at: 0 125 242 701 969 1095 1233 1416 1529
Size: 125 117 459 268 126 138 183 113
Cuts at: 1529 1854
Size: 325
Fragments arranged by size:
459 325 268 183 138 126 125 117
113
BspEI T'CCGG_A
Cuts at: 0 215 1854
Size: 215 1639
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 315 1854
Size: 315 1539
BsrGI T'GTAC_A
Cuts at: 0 26 791 1208 1854
Size: 26 765 417 646
Fragments arranged by size:
765 646 417 26
BssSI C'ACGA_G
Cuts at: 0 1226 1754 1854
Size: 1226 528 100
Fragments arranged by size:
1226 528 100
BstAPI GCAn_nnn'nTGC
Cuts at: 0 315 390 1854
Size: 315 75 1464
Fragments arranged by size:
1464 315 75
BstYI r'GATC_y
Cuts at: 0 623 1100 1660 1854
Size: 623 477 560 194
Fragments arranged by size:
623 560 477 194
BtgI C'CryG_G
Cuts at: 0 596 1854
Size: 596 1258
BtsI GCAGTG_nn'
Cuts at: 0 594 1680 1722 1833 1854
Size: 594 1086 42 111 21
Fragments arranged by size:
1086 594 111 42 21
DraI TTT'AAA
Cuts at: 0 1667 1854
Size: 1667 187
EaeI y'GGCC_r
Cuts at: 0 371 761 1790 1854
Size: 371 390 1029 64
Fragments arranged by size:
1029 390 371 64
EarI CTCTTCn'nnn_
Cuts at: 0 391 1854
Size: 391 1463
EcoICRI GAG'CTC
Cuts at: 0 240 967 1854
Size: 240 727 887
Fragments arranged by size:
887 727 240
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 169 178 247 442 463 722 739 748
Size: 169 9 69 195 21 259 17 9
Cuts at: 748 1310 1759 1854
Size: 562 449 95
Fragments arranged by size:
562 449 259 195 169 95 69 21
17 9 9
EcoNI CCTnn'n_nnAGG
Cuts at: 0 250 1610 1854
Size: 250 1360 244
Fragments arranged by size:
1360 250 244
EcoO109I rG'GnC_Cy
Cuts at: 0 714 723 1120 1854
Size: 714 9 397 734
Fragments arranged by size:
734 714 397 9
FspI TGC'GCA
Cuts at: 0 323 1854
Size: 323 1531
HaeII r_GCGC'y
Cuts at: 0 684 1854
Size: 684 1170
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 30 187 219 1209 1241 1315 1347 1854
Size: 30 157 32 990 32 74 32 507
Fragments arranged by size:
990 507 157 74 32 32 32 30
HindIII A'AGCT_T
Cuts at: 0 52 107 1854
Size: 52 55 1747
Fragments arranged by size:
1747 55 52
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 26 215 1405 1854
Size: 26 189 1190 449
Fragments arranged by size:
1190 449 189 26
MscI TGG'CCA
Cuts at: 0 373 763 1854
Size: 373 390 1091
Fragments arranged by size:
1091 390 373
MslI CAynn'nnrTG
Cuts at: 0 22 159 287 587 1007 1279 1854
Size: 22 137 128 300 420 272 575
Fragments arranged by size:
575 420 300 272 137 128 22
MspA1I CmG'CkG
Cuts at: 0 598 1854
Size: 598 1256
NsiI A_TGCA'T
Cuts at: 0 1286 1854
Size: 1286 568
NspI r_CATG'y
Cuts at: 0 15 1288 1854
Size: 15 1273 566
Fragments arranged by size:
1273 566 15
PasI CC'CwG_GG
Cuts at: 0 559 1854
Size: 559 1295
PciI A'CATG_T
Cuts at: 0 11 1854
Size: 11 1843
PflMI CCAn_nnn'nTGG
Cuts at: 0 654 1854
Size: 654 1200
PpuMI rG'GwC_Cy
Cuts at: 0 723 1120 1854
Size: 723 397 734
Fragments arranged by size:
734 723 397
PstI C_TGCA'G
Cuts at: 0 395 1449 1854
Size: 395 1054 405
Fragments arranged by size:
1054 405 395
SacI G_AGCT'C
Cuts at: 0 242 969 1854
Size: 242 727 885
Fragments arranged by size:
885 727 242
SacII CC_GC'GG
Cuts at: 0 599 1854
Size: 599 1255
SexAI A'CCwGG_T
Cuts at: 0 653 1854
Size: 653 1201
SfcI C'TryA_G
Cuts at: 0 391 1253 1445 1854
Size: 391 862 192 409
Fragments arranged by size:
862 409 391 192
SmaI CCC'GGG
Cuts at: 0 1779 1854
Size: 1779 75
SmlI C'TyrA_G
Cuts at: 0 1096 1854
Size: 1096 758
SphI G_CATG'C
Cuts at: 0 1288 1854
Size: 1288 566
SspI AAT'ATT
Cuts at: 0 1078 1854
Size: 1078 776
StyI C'CwwG_G
Cuts at: 0 785 842 1407 1854
Size: 785 57 565 447
Fragments arranged by size:
785 565 447 57
TatI w'GTAC_w
Cuts at: 0 26 791 935 1208 1854
Size: 26 765 144 273 646
Fragments arranged by size:
765 646 273 144 26
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 337 369 1854
Size: 337 32 1485
Fragments arranged by size:
1485 337 32
Tth111I GACn'n_nGTC
Cuts at: 0 1585 1854
Size: 1585 269
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 522 1854
Size: 522 1332
XhoI C'TCGA_G
Cuts at: 0 1096 1854
Size: 1096 758
XmaI C'CCGG_G
Cuts at: 0 1777 1854
Size: 1777 77
ZraI GAC'GTC
Cuts at: 0 1426 1854
Size: 1426 428
Enzymes that do cut:
AatII AclI AcuI AflIII AhdI AleI AlfI AlwNI
ApaLI ApoI AvaI BanII BbsI BcgI BfrBI BglII
Bme1580I BmgBI BmrI BpmI BsaI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiHKAI BsmI BsmBI Bsp1286I BspEI
BspMI BsrGI BssSI BstAPI BstYI BtgI BtsI DraI
EaeI EarI EcoICRI Eco57MI EcoNI EcoO109I FspI HaeII
Hin4I HindIII MmeI MscI MslI MspA1I NsiI NspI
PasI PciI PflMI PpuMI PstI SacI SacII SexAI
SfcI SmaI SmlI SphI SspI StyI TatI TstI
Tth111I XcmI XhoI XmaI ZraI
Enzymes that do not cut:
AarI AccI Acc65I AfeI AflII AgeI AloI ApaI
AscI AseI AsiSI AvrII BaeI BamHI BanI BbeI
BbvCI BciVI BclI BglI BlpI BmtI BplI Bpu10I
BpuEI BsaAI BsaBI BsiEI BsiWI BspHI BsrBI BsrDI
BsrFI BssHII BstBI BstEII BstXI BstZ17I Bsu36I BtgZI
ClaI CspCI DraIII DrdI EagI EciI EcoRI EcoRV
FalI FseI FspAI HincII HpaI KasI KpnI MfeI
MluI NaeI NarI NcoI NdeI NgoMIV NheI NotI
NruI PacI PfoI PmeI PmlI PpiI PshAI PsiI
PspOMI PspXI PsrI PvuI PvuII RsrII SalI SanDI
SapI SbfI ScaI SfiI SfoI SgfI SgrAI SnaBI
SpeI SrfI StuI SwaI TaqII XbaI XmnI