(Circular) (Six-base) MAPSORT of: pFN21AB5339.seq Check: 8711 from: 1 to: 6307 pFN21AB5339 6307 bp With 182 enzymes: SgfI * February 27, 2009 14:34 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2459 2459 Size: 6307 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4117 278 Size: 53 83 186 936 2581 2468 Fragments arranged by size: 2581 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3650 1937 Size: 1713 4594 Acc65I G'GTAC_C Cuts at: 2236 3628 2236 Size: 1392 4915 AclI AA'CG_TT Cuts at: 1563 4434 4807 1563 Size: 2871 373 3063 Fragments arranged by size: 3063 2871 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2550 2757 3251 3570 4370 5754 6022 1632 Size: 918 207 494 319 800 1384 268 1917 Fragments arranged by size: 1917 1384 918 800 494 319 268 207 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6110 AflIII A'CryG_T Cuts at: 1203 2371 5206 6183 1203 Size: 1168 2835 977 1327 Fragments arranged by size: 2835 1327 1168 977 AgeI A'CCGG_T Cuts at: 5138 5138 Size: 6307 AhdI GACnn_n'nnGTC Cuts at: 5036 5036 Size: 6307 AleI CACnn'nnGTG Cuts at: 1166 2765 1166 Size: 1599 4708 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6307 AlwNI CAG_nnn'CTG Cuts at: 3145 4050 5622 3145 Size: 905 1572 3830 Fragments arranged by size: 3830 1572 905 ApaLI G'TGCA_C Cuts at: 4364 5520 4364 Size: 1156 5151 ApoI r'AATT_y Cuts at: 1493 2413 2432 2513 3071 3290 3455 3616 3842 Size: 920 19 81 558 219 165 161 226 Cuts at: 3842 3994 6103 1493 Size: 152 2109 1697 Fragments arranged by size: 2109 1697 920 558 226 219 165 161 152 81 19 AseI AT'TA_AT Cuts at: 160 1032 4861 160 Size: 872 3829 1606 Fragments arranged by size: 3829 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6307 AvaI C'yCGr_G Cuts at: 1870 1943 3632 1870 Size: 73 1689 4545 Fragments arranged by size: 4545 1689 73 BamHI G'GATC_C Cuts at: 3637 4004 3637 Size: 367 5940 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2011 2236 3628 5083 618 Size: 325 217 627 224 225 1392 1455 1842 Fragments arranged by size: 1842 1455 1392 627 325 225 224 217 BanII G_rGCy'C Cuts at: 1391 2641 3626 1391 Size: 1250 985 4072 Fragments arranged by size: 4072 1250 985 BbeI G_GCGC'C Cuts at: 2015 2015 Size: 6307 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 5336 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4498 4532 1903 Size: 34 2561 34 3678 Fragments arranged by size: 3678 2561 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4199 5409 4199 Size: 1210 5097 BclI T'GATC_A Cuts at: 1548 1548 Size: 6307 BfrBI ATG'CAT Cuts at: 2779 2779 Size: 6307 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2009 4918 136 Size: 107 122 71 631 605 337 2909 1525 Fragments arranged by size: 2909 1525 631 605 337 122 107 71 BglII A'GATC_T Cuts at: 6302 6302 Size: 6307 BlpI GC'TnA_GC Cuts at: 2329 3724 2329 Size: 1395 4912 Bme1580I G_kGCm'C Cuts at: 1790 4368 5524 1790 Size: 2578 1156 2573 Fragments arranged by size: 2578 2573 1156 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6307 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2312 4038 4996 Size: 416 53 83 399 24 895 1726 958 Cuts at: 4996 442 Size: 1753 Fragments arranged by size: 1753 1726 958 895 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 2621 1056 Size: 1565 4742 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2213 2213 Size: 6307 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 4967 1239 Size: 3728 2579 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4432 5297 5595 4432 Size: 865 298 5144 Fragments arranged by size: 5144 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4970 882 Size: 617 3471 2219 Fragments arranged by size: 3471 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 4098 493 Size: 711 2894 2702 Fragments arranged by size: 2894 2702 711 BsaBI GATnn'nnATC Cuts at: 6301 6301 Size: 6307 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2012 2027 3161 4114 Size: 53 83 186 936 479 15 1134 953 Cuts at: 4114 4496 5131 275 Size: 382 635 1451 Fragments arranged by size: 1451 1134 953 936 635 479 382 186 83 53 15 BsaWI w'CCGG_w Cuts at: 2361 4740 5138 5412 5559 5970 2361 Size: 2379 398 274 147 411 2698 Fragments arranged by size: 2698 2379 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 3195 3225 1178 Size: 30 165 30 1792 30 4260 Fragments arranged by size: 4260 1792 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2123 3331 1186 Size: 937 1208 4162 Fragments arranged by size: 4162 1208 937 BseYI C'CCAG_C Cuts at: 2674 3484 5510 6006 2674 Size: 810 2026 496 2975 Fragments arranged by size: 2975 2026 810 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 5734 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3757 4518 5546 1540 Size: 275 186 1756 761 1028 2301 Fragments arranged by size: 2301 1756 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2330 2361 4368 4453 5524 2330 Size: 31 2007 85 1071 3113 Fragments arranged by size: 3113 2007 1071 85 31 BsmI GAATG_Cn' Cuts at: 2548 2776 3818 3911 2548 Size: 228 1042 93 4944 Fragments arranged by size: 4944 1042 228 93 BsmBI CGTCTCn'nnnn_ Cuts at: 2018 2018 Size: 6307 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2330 2361 2641 3626 4368 4453 5524 Size: 399 540 31 280 985 742 85 1071 Cuts at: 5524 1391 Size: 2174 Fragments arranged by size: 2174 1071 985 742 540 399 280 85 31 BspEI T'CCGG_A Cuts at: 5970 5970 Size: 6307 BspHI T'CATG_A Cuts at: 4196 5926 4196 Size: 1730 4577 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2459 3662 844 Size: 1615 1203 3489 Fragments arranged by size: 3489 1615 1203 BsrBI CCG'CTC Cuts at: 1394 1823 4194 1394 Size: 429 2371 3507 Fragments arranged by size: 3507 2371 429 BsrDI GCAATG_nn' Cuts at: 65 1248 3489 4802 4976 65 Size: 1183 2241 1313 174 1396 Fragments arranged by size: 2241 1396 1313 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4951 5138 1700 Size: 110 3141 187 2869 Fragments arranged by size: 3141 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6307 BssHII G'CGCG_C Cuts at: 1925 2080 1925 Size: 155 6152 BssSI C'ACGA_G Cuts at: 4367 5379 4367 Size: 1012 5295 BstAPI GCAn_nnn'nTGC Cuts at: 794 2588 794 Size: 1794 4513 BstBI TT'CG_AA Cuts at: 2511 5111 2511 Size: 2600 3707 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6307 BstYI r'GATC_y Cuts at: 1972 3637 4004 4389 4406 5858 5944 5956 6302 Size: 1665 367 385 17 1452 86 12 346 Cuts at: 6302 1972 Size: 1977 Fragments arranged by size: 1977 1665 1452 385 367 346 86 17 12 Bsu36I CC'TnA_GG Cuts at: 3423 3423 Size: 6307 BtgI C'CryG_G Cuts at: 513 1065 2004 2019 513 Size: 552 939 15 4801 Fragments arranged by size: 4801 939 552 15 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2475 2789 487 Size: 683 56 1249 314 4005 Fragments arranged by size: 4005 1249 683 314 56 BtsI GCAGTG_nn' Cuts at: 3130 3286 3352 3823 4617 4637 3130 Size: 156 66 471 794 20 4800 Fragments arranged by size: 4800 794 471 156 66 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6307 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6272 DraI TTT'AAA Cuts at: 2518 3069 3580 3611 2518 Size: 551 511 31 5214 Fragments arranged by size: 5214 551 511 31 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5314 809 Size: 507 3998 1802 Fragments arranged by size: 3998 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 3127 3754 4643 Size: 54 1425 50 275 18 1297 627 889 Cuts at: 4643 6272 8 Size: 1629 43 Fragments arranged by size: 1629 1425 1297 889 627 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3754 1537 Size: 275 1942 4090 Fragments arranged by size: 4090 1942 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2733 3366 4237 1353 Size: 1380 633 871 3423 Fragments arranged by size: 3423 1380 871 633 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4894 5268 5414 387 Size: 1064 138 3305 374 146 1280 Fragments arranged by size: 3305 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2550 2757 3251 3570 4370 4967 5754 Size: 393 918 207 494 319 800 597 787 Cuts at: 5754 6022 1239 Size: 268 1524 Fragments arranged by size: 1524 918 800 787 597 494 393 319 268 207 EcoRI G'AATT_C Cuts at: 2432 3616 3994 2432 Size: 1184 378 4745 Fragments arranged by size: 4745 1184 378 FspI TGC'GCA Cuts at: 4813 4813 Size: 6307 HaeII r_GCGC'y Cuts at: 1678 2015 5454 1678 Size: 337 3439 2531 Fragments arranged by size: 3439 2531 337 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2204 2236 2588 2620 2714 Size: 32 135 32 632 32 352 32 94 Cuts at: 2714 2746 3195 3227 4948 4980 5022 5054 1373 Size: 32 449 32 1721 32 42 32 2626 Fragments arranged by size: 2626 1721 632 449 352 135 94 42 32 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2482 3651 3897 669 Size: 988 281 544 1169 246 3079 Fragments arranged by size: 3079 1169 988 544 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6307 HpaI GTT'AAC Cuts at: 3897 3897 Size: 6307 KasI G'GCGC_C Cuts at: 2011 2011 Size: 6307 KpnI G_GTAC'C Cuts at: 2240 3632 2240 Size: 1392 4915 MfeI C'AATT_G Cuts at: 3906 3906 Size: 6307 MluI A'CGCG_T Cuts at: 6183 6183 Size: 6307 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2962 3519 5421 5605 1305 Size: 1657 557 1902 184 2007 Fragments arranged by size: 2007 1902 1657 557 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 3129 10 Size: 54 1425 343 1297 3188 Fragments arranged by size: 3188 1425 1297 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2283 2765 2814 2898 4265 4624 4783 Size: 648 1117 482 49 84 1367 359 159 Cuts at: 4783 518 Size: 2042 Fragments arranged by size: 2042 1367 1117 648 482 359 159 84 49 MspA1I CmG'CkG Cuts at: 1606 3327 3723 4400 5548 5793 1606 Size: 1721 396 677 1148 245 2120 Fragments arranged by size: 2120 1721 1148 677 396 245 NarI GG'CG_CC Cuts at: 2012 2012 Size: 6307 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 4816 Fragments arranged by size: 4816 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 6307 NheI G'CTAG_C Cuts at: 1052 2617 1052 Size: 1565 4742 NotI GC'GGCC_GC Cuts at: 3754 3754 Size: 6307 NsiI A_TGCA'T Cuts at: 2781 2781 Size: 6307 NspI r_CATG'y Cuts at: 1132 1229 2375 3665 5210 1132 Size: 97 1146 1290 1545 2229 Fragments arranged by size: 2229 1545 1290 1146 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6307 PciI A'CATG_T Cuts at: 2371 5206 2371 Size: 2835 3472 PflMI CCAn_nnn'nTGG Cuts at: 1108 2840 1108 Size: 1732 4575 PmeI GTTT'AAAC Cuts at: 3611 3611 Size: 6307 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4376 4408 5889 5921 4376 Size: 32 1481 32 4762 Fragments arranged by size: 4762 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6307 PsiI TTA'TAA Cuts at: 2226 3877 2226 Size: 1651 4656 PstI C_TGCA'G Cuts at: 830 2470 3169 3659 830 Size: 1640 699 490 3478 Fragments arranged by size: 3478 1640 699 490 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6307 PvuII CAG'CTG Cuts at: 3327 3327 Size: 6307 SalI G'TCGA_C Cuts at: 1936 3649 1936 Size: 1713 4594 SbfI CC_TGCA'GG Cuts at: 3659 3659 Size: 6307 ScaI AGT'ACT Cuts at: 4555 6267 4555 Size: 1712 4595 SfcI C'TryA_G Cuts at: 826 1045 2343 2466 3165 3655 4790 5471 5662 Size: 219 1298 123 699 490 1135 681 191 Cuts at: 5662 826 Size: 1471 Fragments arranged by size: 1471 1298 1135 699 681 490 219 191 123 SfoI GGC'GCC Cuts at: 2013 2013 Size: 6307 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6307 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6307 SmaI CCC'GGG Cuts at: 1872 3634 1872 Size: 1762 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 4411 5312 5574 820 Size: 197 926 2468 901 262 1553 Fragments arranged by size: 2468 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 4098 493 Size: 3605 2702 SpeI A'CTAG_T Cuts at: 152 3269 152 Size: 3117 3190 SphI G_CATG'C Cuts at: 1132 3665 1132 Size: 2533 3774 SspI AAT'ATT Cuts at: 5 52 4231 5 Size: 47 4179 2081 Fragments arranged by size: 4179 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6307 StyI C'CwwG_G Cuts at: 513 1065 2004 2607 3746 513 Size: 552 939 603 1139 3074 Fragments arranged by size: 3074 1139 939 603 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2666 4334 4351 4504 4689 5134 325 Size: 1255 1086 1668 17 153 185 445 1498 Fragments arranged by size: 1668 1498 1255 1086 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 4553 6265 96 Size: 274 80 33 51 1443 2576 1712 138 Fragments arranged by size: 2576 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 6267 6299 6267 Size: 32 6275 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6307 XbaI T'CTAG_A Cuts at: 3643 3643 Size: 6307 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6307 XmaI C'CCGG_G Cuts at: 1870 3632 1870 Size: 1762 4545 XmnI GAAnn'nnTTC Cuts at: 4436 4436 Size: 6307 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4115 276 Size: 53 83 186 936 2581 2468 Fragments arranged by size: 2581 2468 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoRI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NarI NcoI NdeI NheI NotI NsiI NspI PasI PciI PflMI PmeI PpiI PshAI PsiI PstI PvuI PvuII SalI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI ApaI AscI AvrII BaeI BbvCI Bpu10I BsiWI BstXI BstZ17I DraIII EcoICRI EcoNI EcoO109I EcoRV FalI FseI FspAI NaeI NgoMIV NruI PacI PfoI PmlI PpuMI PspOMI PspXI PsrI RsrII SacI SacII SanDI SapI SexAI SfiI SrfI SwaI XcmI